52results about How to "Easy to extract and purify" patented technology

The invention discloses an armillaria luteo-virens fibrinolytic enzyme and a production method thereof. Fermentation and cultivation are made to armillaria luteo-virens ZJUQH CGMCC No.1884 to get fermented liquid and the armillaria luteo-virens fibrinolytic enzyme is separated and purified from the fermented liquid. The invention first realizes that artificial cultivated armillaria luteo-virens ZJUQH CGMCC No.1884 can be used for producing the fibrinolytic enzyme. The produced armillaria luteo-virens fibrinolytic enzyme takes agricultural and sideline products such as bean pulp, soybean flour and wheat bran with low cost as the main raw materials, thereby being safe and nonpoisonous. Being produced with a liquid fermentation method, the armillaria luteo-virens fibrinolytic enzyme is a hyper strain with relatively higher yield among the reports of epiphytes. Extraction and purification can be carried out easily and the armillaria luteo-virens is harmless, frozen-dry health products that contain thalli and enzyme liquid can be directly developed, thus providing a new thought and an evidence for developing and using novel edible fungus used in foods and medicines.

The invention provides a method for preparing baikal skullcap root chromocor aglycone extract, comprising following steps: disintegrating baikal skullcap root, adding water with amount being 4- 10 times of baikal skullcap root, heating to 40- 70 Deg. C and thermal insulating for 5- 24 hours, enzymolyzing, filtering after reaction, throwing away filtering liquid, extracting dregs of a decoction with ethanol, recovering ethanol, depositing, filtering and drying, getting baikal skullcap root chromocor aglycone extract. The reaction time can be adjusted along with enzymolysis temperature to get optimum enzymolysis effect and increase medicinal materials availability ratio; the water soluble foreign substance can be remove buy filtering hydrolysate, which is convenient for following chromocor aglycone extraction. The invention is characterized by high productivity, simple operation, low cost and suitability for industrial production.

The invention relates to a method for extracting syringin, comprising the following steps of: using Ilex rotunda as material, crushing the material to 20-80 meshes, adding 6-20 folds of water to extract for 2-3 times at 60-80 DEG C; concentrating the extraction liquid under reduced pressure, adding low-carbon alcohol to the extraction liquid for azeotropy, adding 0.5-2% of active carbon for decolorizing; adjusting the pH of decolorized liquid to 1-3, precipitating and filtering the impurity, adjusting the filtrate to be neutral by the aqueous alkali, concentrating the filtrate under reduced pressure until no alcohol exists, adding water to be saturated so as to extract n-butyl alcohol; collecting n-butyl alcohol extract liquor; recovering the n-butyl alcohol under reduced pressure, adding proper amount of water and storing the mixture to form crystal, filtering the crystal; dissolving the crystal with water and acetone solution for re-crystallization; and drying the crystal under reduced pressure to obtain the syringin product. By using the method for extracting the syringin, the one-time investment on devices for the method is less, process operation is simple and product content is high, so that the method is suitable for industrial production.

The invention relates to a method for continuously extracting flaxseed gum and secoisolariciresinol diglucoside from flaxseed meal. The method comprises the following steps: 1) separation of shells and cores of flaxseeds: pulverizing and sieving flaxseed meal, and separately collecting upper-layer flaxseed shells and lower-layer flaxseed cores which are sieved; 2) extraction of flaxseed gum: taking the flaxseed shells as raw materials, and extracting the flaxseed gum with hot water in a microwave assisted manner; 3) extraction of secoisolariciresinol diglucoside: mixing and extracting the degummed flaxseed shells and an ethanol-sodium hydroxide solution to obtain coarse secoisolariciresinol diglucoside; and 4) purification of the secoisolariciresinol diglucoside: purifying the secoisolariciresinol diglucoside coarse product by using macroporous resin to obtain high-purity secoisolariciresinol diglucoside. The flaxseed gum and the secoisolariciresinol diglucoside are continuously extracted from the flaxseed meal, a new way for fully utilizing the flaxseed meal is provided, meanwhile, a convenient and speedy method for preparing various effective constituents of the flaxseeds is provided, flaxseed oil making by-products are utilized effectively, and the production cost of target products is reduced. The method has the advantages of low energy consumption, high product extractingrate, high economic benefit, low environment pollution and the like, and is suitable for large-scale production.

The invention relates to a culture medium recipe for commercial production of rhamnolipid fermentation liquid, which is characterized in coin oil 2-5%, carbamide 0.2-1.5%, molasses 15-20%, potassium chloride 0.05-1.0%, KH2PO40.05-1.2%, K2HPO40.05-1.5%, yeast extract 0.005-0.1%, compound microelements 0.005-0.01% and the rest element is water; the pH value of the invention is 6.5-7.5. The invention has the advantages of low cost, high rhamnolipid content in fermentation, and is suitable for commercial production of rhamnolipid.

The invention discloses a genetically engineered bacterium for producing a linear malt oligosaccharide generating enzyme and application of the genetically engineered bacterium, and belongs to the technical field of microbial engineering. According to the genetically engineered bacterium, secretory expression of the linear malt oligosaccharide generating enzyme in bacillus subtilis is achieved, a method is safe and efficient, the hydrolytic activity reaches 36.6 U/mL, and the enzyme producing capacity is 10 times or above of that of an original bacterium; the obtained recombinant enzyme is an extracellular enzyme, convenience is brought for extraction and purification of large-scale production in the later period, and the product meets the food requirement. Starch is hydrolyzed by the purified recombinant linear malt oligosaccharide generating enzyme to generate linear malt oligosaccharide syrup which does not contain glucose and contains a high proportion of maltopentaose, and the linear malt oligosaccharide syrup can be directly used for producing functional food and also can be used for producing products such as high-purity linear malt oligosaccharides.

The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenient and has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.

The invention discloses a mutant NfpAB nanopore which is a protein complex composed of at least one NfpA protein mutant monomer and at least one NfpB protein mutant monomer, or a protein complex composed of at least two mutant monomers expressed by NfpA and NfpB fusion mutant genes. The nanopore probe has a structure and recognition sites different from those of known nanopores, can be used for recognition, concentration detection and sequencing of polymers such as nucleic acid, polypeptide and protein, allows capture and transfer of single-stranded DNA and distinguishes adenine (A), thymine (T), cytosine (C) and guanine (G). The NfpAB mutant is formed by combining two different monomers, the modification space in a pore channel is enriched, the pore channel has a conical pore channel structure similar to MspA, and the NfpAB mutant is an ideal pore channel for nucleic acid sequencing. The invention also discloses a test system containing the nanopore, a manufacturing method and an application, through engineering site-specific mutagenesis in the nanopore, charge distribution in a pore channel is adjusted, the speed of nucleic acid passing through the pore channel is reduced, and accurate recognition of a basic group is realized.

The invention relates to an effective site group of daphne giraldii nitsche leaf, a preparation method thereof, a medicinal composition containing the effective site group of the daphne giraldii nitsche leaf and application of the effective site group of the daphne giraldii nitsche leaf to the preparation of medicaments for treating diseases such as traumatic injury, arthritis and the like, and belongs to the technical field of pharmaceutics of traditional Chinese medicine. The effective site group of the daphne giraldii nitsche leaf is high in active ingredients and obvious in medicinal effect, medicinal sites do not damage vegetations and environment, the extraction process is easy and convenient, and the operability is high.

The invention relates to the protein expression field and discloses swine vesicular disease virus viral protein 1 (VP1) protein secretion expression recombinant plasmid. The recombinant plasmid is composed of a gene which expresses the swine vesicular disease virus VP1 protein and a melittin signal peptide gene sequence. The recombinant plasmid has the advantages of being capable of achieving secretion and expression of the swine vesicular disease virus VP1 protein outside an insect cell, thereby directly extracting the swine vesicular disease virus VP1 protein from cell culture fluid, effectively improving protein purification efficiency, reducing preparation cost of the swine vesicular disease virus VP1 protein and laying a solid foundation for a research based on the swine vesicular disease virus VP1 protein.

The invention discloses a method for dewaxing and decoloring cannabidiol extract, which is characterized by comprising the following steps: (1) pretreatment of raw materials: crushing industrial hempflower leaves, conducting baking for decarboxylation, and conducting extracting with an extracting agent at normal temperature to obtain crude extract; (2) dewaxing: dissolving the extract crude extract with ethanol to obtain an extract mixed solution, then adding a filter aid into the extract mixed solution, and carrying out freezing, cold filtration, washing, evaporation and concentration to obtain a wax-free extract crude product; and (3) decolorization: dissolving the wax-free extract crude product with ethanol to obtain a dissolved solution, adding a decolorizing agent into the dissolvedsolution, and conducting decolorizing, filtering, washing, filtering again, evaporating and concentrating to obtain golden yellow or yellowish-brown wax-free CBD extract. The method has the advantagesof simplicity in operation, lower requirement on personnel, small solvent consumption, low equipment requirement, remarkable dewaxing and decoloring effects, reasonable process, environmental protection and high efficiency.

The invention provides a method for extracting carotenoid from a microbial fermentation source, comprising the following steps of: (1) carrying out spray drying on a microbial fermentation solution containing carotenoid to obtain first bacterial powder; (2) carrying out wall breaking on the dry bacterial powder by using a wall breaking machine to obtain second bacterial powder; (3) carrying out saponification treatment on the wall-broken bacterial powder, and filtering to obtain third bacterial powder; (4) extracting the third bacterial powder, filtering, and collecting a filtrate; and (5) concentrating the filtrate to obtain a concentrated solution, carrying out low-temperature stirring crystallization, adding a small amount of absolute ethyl alcohol for rinsing, filtering to obtain crystals, and carrying out vacuum drying on the crystals to obtain pure carotenoid crystals. According to the method, the wall breaking rate reaches 99%, the extraction rate reaches 98% or above, the totalyield reaches 90% or above, the technological operation is simple, the conditions are mild, the carotenoid loss is small, the product quality is stable, the solvent recovery rate is high, the cost iseffectively reduced, and the method is suitable for industrial production.

The invention provides a method for extracting and purifying pravastatin sodium from a fermentation liquor, and belongs to the field of biological medicine preparation. According to the method for extracting and purifying pravastatin sodium from the fermentation liquor, a large amount of impurities and pigments are removed by alkalizing and acidifying the fermentation liquor, and the effective components in an acidizing liquid are enriched by an adsorbent, so that the purity of an extraction substrate is improved, the quantity of the extraction substrate is greatly reduced, and the quality ofan extraction liquid is further improved; in the whole production process, a single solvent system is adopted, the process is not complicated, the generated three wastes are low in amount, and the method is suitable for large-scale production. The content of pravastatin sodium produced by the method is more than 98.8 percent, the content of main impurity 6-epipravastatin is less than 0.15%, the content of total related substances is less than 0.4%, the product quality accords with the EP9.6 edition quality standard (the content of the pravastatin sodium is between 97.0% and 102.0%; a main impurity A, namely6-epipravastatin, is less than or equal to 0.3%; the content of the total related substances is less than or equal to 0.6%) of qualified pravastatin sodium, the total yield can reach more than 75%, and the large-scale production prospect is good.

The invention discloses an extraction method of Araceae polysaccharide, which comprises the following steps: soaking Araceae in a sodium chloride solution, carrying out ultrasonic treatment, treatingin a dialysis bag, pouring out liquid in the dialysis bag, carrying out distillation treatment, adding an ethanol solution, and carrying out repeated purification to obtain the Araceae polysaccharide.The operation method is simple, and cell walls of Araceae are broken by utilizing a sodium chloride solution, so that contents in cells are dissolved out; rapidly concentrating of the filtrate is carried out by using the dialysis bag to obtain a liquid containing less impurities; purifying is carried out for multiple times by utilizing an ethanol solution to obtain the high-purity Araceae polysaccharide.

The invention relates to an escherichia coli fermentation medium and a fermentation technology for producing succinic acid. A formula of the medium comprises 1.4-1.6g/dL corn steep liquor dry powder,0.5-2.5g/L yeast extract, 0.2-1.4g/L MgSO4, 2.5-4.5g/L (NH4)2HPO4, 0.6-2.0g/L C5H11NO2.HCl, 0.2-1.0g/L KCl, 50-60mg/L CuSO4, and 35-50mg/L FeSO4.7H2O; a pH of the medium is 6.5-6.8; and a required volume is reached by distilled water. The fermentation technology comprises the steps of taking escherichia coli as a fermentation strain, and performing aerobic enrichment in a primary seeding tank anda secondary seeding tank, and anaerobic fermentation in a fermentation tank to form succinic acid, wherein a formula of the fermentation medium is the above formula. According to the medium and the technology, the total fermentation cost can be lowered by 22%.

The invention relates to the technical field of biology, in particular to a culture medium for promoting the synthesis of antibacterial polypeptide gramicidin and a culture method thereof. The culture medium comprises the following raw materials in parts by weight: 15-25 parts of carbon source, 2-3 parts of nitrogen source, 1-3 parts of K2HPO4 and 1-3 parts of MgSO4.7H2O. In the aspect of promoting the synthesis of the linear short-stem peptide in the bacillus brevis, compared with conventional NA and NB culture mediums, the finished product of SAM liquid culture medium can greatly promote the expression of the linear gramicidin synthetase coding gene, and the expression amount is about 7-9 times of that of the conventional NB liquid culture medium, so that the synthesis amount of the linear gramicidin is greatly improved, and for specific performance, the inhibition zone on the pathogenic bacteria of Candida albicans is increased. The linear gramicidin cultured by the culture method not only can meet the research requirements, but also can promote the extraction, purification, development and application of the linear gramicidin.

The invention provides an application of soybean RNA extract in preparation of a medicine for preventing and treating enteritis, and belongs to the technical field of biology. In-vitro experiments show that the soybean RNA extract and gma-miR159a have no effect on proliferation activity of normal colonic epithelial cells, and have good safety; in-vivo experiments show that the soybean RNA can significantly improve the colon pathology and inflammation level of model mice with colitis, and improve the health level of the mice; therefore, the soybean RNA extract and gma-miR159a can effectively prevent and treat enteritis-related diseases. The soybean RNA extract and gma-miR159a are directly derived from soybeans, and have specific targeting, and the characteristics of safety, high efficiencyand wide sources; and the soybean RNA extract and gma-miR159a are easy to extract, have broad prospects in the fields of food and medicine, and can be used to prepare the medicine or functional foodsfor preventing and treating the enteritis.