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Culture medium prescription for the industrial production of rhamnolipid fermentation liquor

A medium formulation and a rhamnolipid technology are applied in the field of medium formulations for industrial production of rhamnolipid fermentation broth, and can solve problems such as failure to form an industrialized production scale, low rhamnolipid content, and optimized medium formulations. , to achieve the effect of shortening the fermentation cycle, increasing the amount of rhamnolipid, and shortening the cycle

Inactive Publication Date: 2008-05-07
DAQING VERTEX CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, rhamnolipids have always been in the stage of indoor research, and no industrial production scale has been formed, and there is no optimized formula for the medium that matches the production of rhamnolipids by industrial methods, and the cost of the existing medium is high, and the fermented rat low in glycolipids

Method used

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  • Culture medium prescription for the industrial production of rhamnolipid fermentation liquor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1, industrialized method produces rhamnolipid biological fermentation liquid:

[0010] Shake flask and fermenter culture: Pseudomonas aeruginosa (Pseudomonas sp.VTS-1) was cultured for 30 hours, transferred to a 5L shake flask (loaded with 2L medium), and the inoculation amount was 5% (the concentration of the bacterial solution was at OD 600 =0.8~1.0), the temperature is 32±2°C, 120rpm cultivated for 12h, transferred to the 500-liter first-level fermenter, the filling coefficient is 75%, the inoculum size is 5%, the temperature is 32±2°C, and the ventilation ratio is 1:1, pH 6.5-7.5, stirring speed 100rpm, culture for 12 hours, transfer to a 3.5-ton secondary fermentation tank, the filling coefficient is 75%, the inoculum size is 5%, the temperature is 32±2°C, and the ventilation ratio is 1 : 1. The pH is 6.5-7.5, the stirring speed is 100rpm and cultivated for 12 hours, then transferred to a 30-ton three-stage fermenter with a loading capacity of 20 tons. T...

Embodiment 2

[0012] Embodiment 2, industrialized method produces rhamnolipid biological fermentation liquid:

[0013] Shake flask and fermenter culture: Pseudomonas sp.VTS-1 was cultured for 30 hours, transferred to a 5L shake flask (loaded with 2L medium), inoculated at 5%, 32±2°C, 120rpm and cultured for 12 hours , the 500 liters of first-class fermentation tanks that are transferred into, the filling coefficient is 65%, the inoculum size is 5%, the ventilation ratio is 1:1, the pH is 6.5~7.5, the stirring speed is 100rpm, and the inoculum size is 5%, and the stirring speed is 100rpm. Connect to a 3.5-ton secondary fermentation tank, with a filling factor of 65%, an inoculum size of 5%, 32±2°C, a ventilation ratio of 1:1, a pH of 6.5-7.5, and a stirring speed of 100-150rpm for 12 hours, transfer Enter a 30-ton three-stage fermenter with a loading capacity of 20 tons. The process parameters of the three-stage fermenter are: temperature 32±2°C, pH adjusted to about 6 before sterilization, ...

Embodiment 3

[0015] Embodiment 3, industrialized method produces rhamnolipid biological fermentation liquid:

[0016] Shake flask and fermenter culture: Pseudomonas sp.VTS-1 was cultured for 30 hours, transferred to a 5L shake flask (loaded with 2L medium), inoculated at 5%, 32±2°C, 120rpm and cultured for 14 hours , transferred to a 500-liter primary fermenter with a filling factor of 65%, an inoculum size of 5%, 32±2°C, a ventilation ratio of 1:1, a pH of 6.5 to 7.5, and a stirring speed of 100 to 150 rpm for 12 hours. , transferred to 3.5 tons of secondary fermenter (filling coefficient is 65-75%), inoculum size 5%, 32 ± 2 ℃, ventilation ratio is 1: 1, pH is 6.5-7.5, stirring speed is 100rpm to cultivate 12h , transferred to a 30-ton three-stage fermenter with a loading capacity of 18 tons. The process parameters of the three-stage fermenter are: temperature 32±2°C, pH adjusted to about 6 before sterilization, and pH control with 0.185 tons of NaOH in the later stage Above 6, the venti...

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Abstract

The invention relates to a culture medium recipe for commercial production of rhamnolipid fermentation liquid, which is characterized in coin oil 2-5%, carbamide 0.2-1.5%, molasses 15-20%, potassium chloride 0.05-1.0%, KH2PO40.05-1.2%, K2HPO40.05-1.5%, yeast extract 0.005-0.1%, compound microelements 0.005-0.01% and the rest element is water; the pH value of the invention is 6.5-7.5. The invention has the advantages of low cost, high rhamnolipid content in fermentation, and is suitable for commercial production of rhamnolipid.

Description

Technical field: [0001] The invention relates to a culture medium used in the production of biosurfactants, in particular to a culture medium formula for industrial production of rhamnolipid fermentation liquid. Background technique: [0002] Rhamnolipid belongs to a kind of glycolipid surfactant, which is an extracellular metabolite produced by microorganisms growing to a certain stage in the fermentation process under suitable conditions. It is a kind of biosurfactant with better effect. Like other synthetic surfactants, its molecular structure has both hydrophilic and hydrophobic properties, and can reduce surface tension. At the same time, it has its own characteristics: high biodegradability and lower biotoxicity, Low critical micelle concentration and higher surface activity, gradual absorption, continuous activity and other characteristics. At present, rhamnolipids have always been in the stage of indoor research, and no industrial production scale has been formed, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/02C12R1/385
Inventor 金艳芳李玉梅马艳玲韩立滨孙杨陈韶军
Owner DAQING VERTEX CHEM
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