Culture medium prescription for the industrial production of rhamnolipid fermentation liquor

A medium formulation and a rhamnolipid technology are applied in the field of medium formulations for industrial production of rhamnolipid fermentation broth, and can solve problems such as failure to form an industrialized production scale, low rhamnolipid content, and optimized medium formulations. , to achieve the effect of shortening the fermentation cycle, increasing the amount of rhamnolipid, and shortening the cycle

Inactive Publication Date: 2008-05-07
DAQING VERTEX CHEM
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, rhamnolipids have always been in the stage of indoor research, and no industrial production scale has been formed, and there is no optimized formula for

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  • Culture medium prescription for the industrial production of rhamnolipid fermentation liquor

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Example Embodiment

[0009] Example 1. Production of Rhamnolipid Biological Fermentation Liquid by Industrialized Method:

[0010] Shake flask and fermenter culture: Pseudomonas sp.VTS-1 was cultured for 30h, then transferred to 5L shake flask (filled with 2L medium), the inoculum volume was 5% (the concentration of the bacterial solution was at OD 600 =0.8~1.0), the temperature is 32±2℃, 120rpm culture for 12h, the 500-liter first-stage fermenter connected to the connection, the charging factor is 75%, the inoculum amount is 5%, the temperature is 32±2℃, and the ventilation ratio is 1:1. The pH is 6.5~7.5, the stirring speed is 100rpm, and the culture is for 12h, then it is connected to a 3.5-ton secondary fermentation tank, the charging factor is 75%, the inoculation amount is 5%, the temperature is 32±2℃, and the ventilation ratio is 1. :1. The pH is 6.5~7.5, the stirring speed is 100rpm, the culture is 12h, and it is connected to a 30-ton tertiary fermentation tank with a filling volume of 20 tons...

Example Embodiment

[0012] Example 2. Production of Rhamnolipid Biological Fermentation Broth by Industrialized Method:

[0013] Shake flask and fermenter culture: Pseudomonas sp.VTS-1 (Pseudomonas sp.VTS-1) cultured for 30h, transferred to 5L shake flask (filled with 2L medium), inoculation volume 5%, 32±2℃, 120rpm culture for 12h , Transfer to a 500-liter one-stage fermenter, with a charging factor of 65%, an inoculum of 5%, 32±2°C, a ventilation ratio of 1:1, a pH of 6.5-7.5, and a stirring speed of 100rpm. Connected to a 3.5-ton secondary fermentation tank with a charging factor of 65%, an inoculum of 5%, 32±2°C, a ventilation ratio of 1:1, a pH of 6.5 to 7.5, and a stirring speed of 100 to 150 rpm for 12 hours of cultivation, transfer Into a 30-ton tertiary fermentation tank with a filling volume of 20 tons. The process parameters of the tertiary fermentation tank are: temperature 32±2°C, pH adjusted to about 6 before sterilization, and 0.210 tons of NaOH to control the pH above 6 in the later s...

Example Embodiment

[0015] Example 3. Industrialized method to produce rhamnolipid biological fermentation broth:

[0016] Shake flask and fermenter culture: Pseudomonas sp.VTS-1 (Pseudomonas sp.VTS-1) was cultured for 30h, then transferred to 5L shake flask (filled with 2L medium), inoculation volume 5%, 32±2℃, 120rpm culture for 14h , Transfer to a 500-liter first-level fermenter with a charging factor of 65%, an inoculum of 5%, 32±2°C, a ventilation ratio of 1:1, a pH of 6.5 to 7.5, and a stirring speed of 100 to 150 rpm for 12 hours , Transfer to 3.5 tons of secondary fermentation tank (filling coefficient is 65-75%), inoculum amount is 5%, 32±2℃, ventilation ratio is 1:1, pH is 6.5-7.5, stirring speed is 100rpm and cultivated for 12h , Switch to a 30-ton tertiary fermentation tank with a filling volume of 18 tons. The process parameters of the tertiary fermentation tank are: temperature 32±2℃, pH adjusted to about 6 before sterilization, and pH control with 0.185 tons of NaOH in the later stage ...

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Abstract

The invention relates to a culture medium recipe for commercial production of rhamnolipid fermentation liquid, which is characterized in coin oil 2-5%, carbamide 0.2-1.5%, molasses 15-20%, potassium chloride 0.05-1.0%, KH2PO40.05-1.2%, K2HPO40.05-1.5%, yeast extract 0.005-0.1%, compound microelements 0.005-0.01% and the rest element is water; the pH value of the invention is 6.5-7.5. The invention has the advantages of low cost, high rhamnolipid content in fermentation, and is suitable for commercial production of rhamnolipid.

Description

Technical field: [0001] The invention relates to a culture medium used in the production of biosurfactants, in particular to a culture medium formula for industrial production of rhamnolipid fermentation liquid. Background technique: [0002] Rhamnolipid belongs to a kind of glycolipid surfactant, which is an extracellular metabolite produced by microorganisms growing to a certain stage in the fermentation process under suitable conditions. It is a kind of biosurfactant with better effect. Like other synthetic surfactants, its molecular structure has both hydrophilic and hydrophobic properties, and can reduce surface tension. At the same time, it has its own characteristics: high biodegradability and lower biotoxicity, Low critical micelle concentration and higher surface activity, gradual absorption, continuous activity and other characteristics. At present, rhamnolipids have always been in the stage of indoor research, and no industrial production scale has been formed, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/02C12R1/385
Inventor 金艳芳李玉梅马艳玲韩立滨孙杨陈韶军
Owner DAQING VERTEX CHEM
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