A kind of extraction method of biological fermentation carotenoid

A carotene and extraction method technology, applied in chemical instruments and methods, hydrocarbons, extraction purification/separation, etc., can solve the problems of increasing oxidation risk, increasing the difficulty of carotenoid extraction process, reducing purity, etc.

Active Publication Date: 2022-04-29
HEC PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Incomplete wall breaking will increase the difficulty of the carotenoid extraction process and limit its extraction yield; in addition, because carotenoids are reducing substances and are easily oxidized, exposure to unstable environments for a long time during the wall breaking process will increase its oxidation risk, reducing its purity

Method used

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  • A kind of extraction method of biological fermentation carotenoid
  • A kind of extraction method of biological fermentation carotenoid
  • A kind of extraction method of biological fermentation carotenoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] A method for extracting biologically fermented carotenoids, using Saccharomyces cerevisiae fermented liquid expressing lycopene as a raw material for extraction, specifically:

[0085] (1) Spray-dry the 20L lycopene fermented liquid after fermentation and putting it into the tank, set the inlet air temperature to 190°C, and the outlet air temperature to 90°C, spray dry to obtain 1kg of dry yeast powder;

[0086] (2) The dry yeast powder is humidified with purified water and adjusted to a water content of 10%, and then the wall is broken by a low-temperature shearing machine for 30 minutes. The cooling form is closed circulating water cooling, and the temperature is 8°C. The wall-broken bacteria powder is collected, and the wall-breaking rate is greater than 98% after testing, and the loss of lycopene content is less than 2%;

[0087] (3) Add 10 L of ethanol solution with a sodium hydroxide content of 1% to the dried bacterial powder after the wall breaking for saponific...

Embodiment 2

[0093] A method for extracting biologically fermented carotenoids, using Saccharomyces cerevisiae fermented liquid expressing β-carotene as a raw material for extraction, specifically:

[0094] (1) Spray-dry the 22L beta-carotene fermented liquid after fermentation and putting into the tank, set the air inlet temperature to 180°C, and the air outlet temperature to 80°C, spray dry to obtain 1.32kg of dry yeast powder;

[0095](2) The dry yeast powder is humidified with purified water and adjusted to a water content of 10%, and then the wall is broken by a low-temperature shearing breaker for 45 minutes. The cooling form is closed circulating water cooling, and the temperature is 4°C. Collect the broken wall bacteria powder, the broken wall rate is more than 99% after testing, and the loss of β-carotene content is less than 1.5%;

[0096] (3) Add 6.6L of ethanol solution with a sodium hydroxide content of 2% to the dried bacteria powder after the wall breaking for saponification...

Embodiment 3

[0102] A method for extracting biologically fermented carotenoids, using astaxanthin-expressing Saccharomyces cerevisiae fermentation broth as raw material for extraction, specifically:

[0103] (1) Spray-dry the 25L astaxanthin fermented liquid after fermentation and putting it into the tank, set the air inlet temperature to 190°C, and the air outlet temperature to 90°C, and spray dry to obtain 1.4kg of dry yeast powder;

[0104] (2) The dry yeast powder is humidified with purified water and adjusted to a water content of 9%, and then the wall is broken by a low-temperature shearing breaker for 60 minutes. The cooling form is closed circulating water cooling, and the temperature is 0°C. The wall-broken bacteria powder is collected, and the wall-breaking rate is greater than 99% after testing, and the loss of astaxanthin content is less than 2.5%;

[0105] (3) Add 8.4 L of ethanol solution with a sodium hydroxide content of 3% to the dried bacterial powder after the wall break...

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Abstract

The invention provides a method for extracting carotenoids derived from microbial fermentation, the method comprising: (1) spray-drying microbial fermentation liquid containing carotenoids to obtain a first bacterial powder; (2) drying the dried The bacteria powder is broken by a wall-breaking machine to obtain the second bacteria powder; (3) saponifying the broken-wall bacteria powder and filtering to obtain the third bacteria powder; (4) extracting the third bacteria powder and filtering , collecting the filtrate; (5) Concentrating the filtrate to obtain a concentrated solution, stirring and crystallizing at low temperature, adding a small amount of absolute ethanol to rinse, filtering to obtain crystals, and vacuum-drying the crystals to obtain pure carotenoid crystals. The method has a wall breaking rate of 99%, an extraction rate of over 98%, and a total yield of over 90%. The process is simple in operation, mild in conditions, less in carotenoid loss, stable in product quality, and high in solvent recovery, effectively reducing The cost is reduced, and it is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of microbial active components. More specifically, it relates to a method for extracting carotenoids from microbial fermentation. Background technique [0002] Carotenoids are a general term for a class of important natural pigments, which are commonly found in yellow, orange-red or red pigments of animals, higher plants, fungi, and algae. It is an isopentenoid polymer containing 40 carbons, namely tetraterpenoids. A typical carotenoid is formed by connecting 8 isoprene units end to end. The color of carotenoids varies with the number of conjugated double bonds. The more the number of conjugated double bonds, the more the color shifts toward red. So far, more than 700 natural carotenoids have been discovered, which can be divided into two categories according to their chemical structures. Carotene and lycopene), the other is the lutein family (with oxygen-containing function...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54C07C403/24C07C7/00C07C7/148C07C7/10C07C7/14C07C11/21
CPCC09B61/00C09B67/0096C07C403/24C07C7/005C07C7/1485C07C7/10C07C7/14C07C2601/16C07C11/21
Inventor 郭振权雷杰进马梅吴广进庞嘉颖蔡燕丰姚红涛谢文平
Owner HEC PHARM CO LTD
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