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Application of soybean RNA extract in preparation of medicine for preventing and treating enteritis

A technology of extract and enteritis, applied in the application field of medicine, can solve the problems of lack of high efficiency, safety prevention and treatment of enteritis, achieve the effect of improving colon pathology and inflammation level, wide source, simple extraction and purification

Active Publication Date: 2020-06-09
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is a lack of efficient and safe drugs for the prevention and treatment of enteritis

Method used

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  • Application of soybean RNA extract in preparation of medicine for preventing and treating enteritis
  • Application of soybean RNA extract in preparation of medicine for preventing and treating enteritis
  • Application of soybean RNA extract in preparation of medicine for preventing and treating enteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of soybean RNA extract and identification of miRNAs

[0029] Soybean RNA extracts were obtained from soybean seeds by conventional methods. The specific method is as follows: soybean seeds are quickly ground in liquid nitrogen, and the obtained soybean powder is immediately transferred to a centrifuge tube containing Trizol reagent (purchased from Invitrogen, USA), wherein the total volume of soybean powder cannot exceed 10% of the volume of Trizol used. %. Place at room temperature for 10 min, then add 1 / 5 of the volume of Trizol reagent in chloroform, cap the centrifuge tube tightly, shake vigorously for 15 s, place at room temperature for 5 min, and centrifuge at 12,000 g for 15 min at 4°C. After centrifugation, the liquid is divided into three layers (colorless liquid in the upper layer is RNA, white in the middle layer is DNA, and red in the bottom layer is protein). Carefully absorb the colorless liquid in the upper layer and transfer it int...

Embodiment 2

[0034] Example 2 Effect of soybean RNA extract and gma-miR159a on the proliferation activity of normal colonic epithelial cells NCM460

[0035] The effects of the soybean RNA extract prepared in Example 1 and the gma-miR159a prepared by chemical synthesis on the proliferation activity of normal colonic epithelial cells NCM460 were detected by MTT assay.

[0036] NCM460 cells were cultured in 96-well plates with 3 × 10 3 cells, soybean RNA group, gma-miR159a group, control group and normal group were respectively provided with 6 wells. Among them, soybean RNA extract with a final concentration of 40 μg / mL was added to each well of the soybean RNA group, gma-miR159a with a final concentration of 50 nM was added to each well of the gma-miR159a group, and gma-miR159a with a final concentration of 50 nM was added to each well of the control group. Scrambled miRNA mimics, normal group without any drug added. Another blank group was set up without cells. After 24 h, 48 h and 72 h of...

Embodiment 3

[0038] Example 3 Effect of Soybean RNA Extract and gma-miR159a on Colitis Model Mice

[0039] (1) Modeling and administration

[0040] Six-week-old male C57BL / 6 mice were purchased from Puhe Biomedical Technology Co., Ltd. (Wuxi, China). 50 mice were randomly divided into 5 groups, 10 in each group, which were normal group, model group, model+scrambled RNA group, model+RNA group and model+gma-miR159a group. In addition to the normal group, the other groups used the following method to establish a colitis mouse model: the experimental mice drank 2% DSS (dextran sodium sulfate) aqueous solution freely for 7 days; then drank normal drinking water (double distilled water) for 14 sky. The above process was repeated for three cycles to construct a colitis mouse model.

[0041] In the process of establishing the colitis mouse model, the mice in the model group were given no other drugs; the mice in the model + RNA group were given the colitis model drugs, and were given soybean RN...

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Abstract

The invention provides an application of soybean RNA extract in preparation of a medicine for preventing and treating enteritis, and belongs to the technical field of biology. In-vitro experiments show that the soybean RNA extract and gma-miR159a have no effect on proliferation activity of normal colonic epithelial cells, and have good safety; in-vivo experiments show that the soybean RNA can significantly improve the colon pathology and inflammation level of model mice with colitis, and improve the health level of the mice; therefore, the soybean RNA extract and gma-miR159a can effectively prevent and treat enteritis-related diseases. The soybean RNA extract and gma-miR159a are directly derived from soybeans, and have specific targeting, and the characteristics of safety, high efficiencyand wide sources; and the soybean RNA extract and gma-miR159a are easy to extract, have broad prospects in the fields of food and medicine, and can be used to prepare the medicine or functional foodsfor preventing and treating the enteritis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of soybean RNA extract in the preparation of medicines for preventing and treating enteritis. Background technique [0002] Enteritis diseases are frequently-occurring and commonly encountered diseases, which seriously affect people's health. These diseases are stubborn and difficult to heal, have a long course of disease, and are prone to repeated attacks. Among them, colitis is more common in intestinal inflammatory diseases, which can be divided into two categories: acute phase and chronic phase. If patients in the acute stage are properly controlled, their symptoms can be relieved and finally cured. Otherwise, the inflammation will recur and eventually become chronic, increasing the risk of colon cancer. It is one of the most serious diseases of the gastrointestinal tract and is recognized by the World Health Organization. Organizations are listed as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61P1/00A23L33/13
CPCA61K31/7088A61P1/00A23L33/13A23V2002/00A23V2200/30
Inventor 沈新春刘峻呈汪芳宋海沼黄宽晨
Owner NANJING UNIV OF FINANCE & ECONOMICS
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