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Preparation method and application of poplar transformed product for improving enteritis

A technology for transformed products and poplars, applied in the field of preparation of poplar transformed products, can solve problems such as lack of core technologies for intensive and deep processing, and achieve the effects of cheap raw material sources, unique components, and rich raw material sources

Inactive Publication Date: 2021-09-07
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on poplar waste lacks both development and utilization oriented to clear active functions, and also lacks effective core technology for deep processing

Method used

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  • Preparation method and application of poplar transformed product for improving enteritis
  • Preparation method and application of poplar transformed product for improving enteritis
  • Preparation method and application of poplar transformed product for improving enteritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Using poplar wood chips (PM) and poplar leaves (PL) as raw materials, dry poplar wood chips and poplar leaves at 60°C for 8 hours, crush them through a 200-mesh sieve, and collect poplar wood chips and poplar leaves pink;

[0034] (2) Use 90% ethanol solution as solvent, extract poplar sawdust powder and poplar leaf powder under reflux at 60°C respectively, the ratio of solid to liquid is 1:5, the extraction time is 2.5h, extract 3 times, then suction filter and reduce pressure Evaporate to obtain extract-like substance;

[0035] (3) Water-soluble extract-like substance, the extract is extracted with petroleum ether and ethyl acetate in sequence, the petroleum ether extraction time is 4h, and the extraction is 3 times, and the remaining solution is extracted with ethyl acetate, the extraction time is 4h, and the extraction is 3 times Collect and evaporate under reduced pressure to obtain poplar extract;

[0036] (4) Total ethanol extract (EE), petroleum ether extr...

Embodiment 2

[0040] (1) Select 11 enzymes produced in the laboratory: xylosidase Xin-Dt from D.thermophilum (optimum temperature 75°C, optimum pH 6.0) and xylosidase TtGH39 from T. optimum temperature 50°C, optimum pH 5.5), α-rhamnosidase AMIHA from A. mulundensis (optimum temperature 65°C, optimum pH 6.0) and α-rhamnoside from D.thermophilum DSM3960 The enzyme DthRha (optimum temperature 90°C, optimum pH 6.5), and 7 kinds of β-glucosidases are Dth3 (optimum temperature 85°C, optimum pH 5.5) from D.thermophilum DSM3960, source Tpebgl1 from T.petrophlia DSM 13995 (optimum temperature 90°C, optimum pH 6.0), Sisbgl1 from S. islandicus (optimum temperature 90°C, optimum pH 5.5), Tthbgl1 from T.thermarum DSM5069T ( optimum temperature 90°C, optimum pH 4.8), Tthbgl3 from T.thermarum DSM5069T (optimum temperature 90°C, optimum pH 5.0), Tpebgl3 from T.petrophlia DSM 13995 (optimum temperature 90°C, optimum optimum pH 5.0) and Anibgl1 derived from A. niger NL-1 (optimum temperature 50°C, optimum p...

Embodiment 3

[0045] Get the ethyl acetate extract phase (EAE) of poplar sawdust and poplar leaf in 20mg embodiment 1, be dissolved in 155 μ l distilled water, add 50 μ l pH 5.5 phosphate buffer and 245 μ l β-glucosidase Dth3 (enzyme activity 40U / ml), respectively The poplar extract was catalyzed by water bath reaction at 85°C, 90°C, and 95°C for 30 minutes, and the catalyzed product was collected.

[0046] The composition and content changes of the extract before and after the catalytic reaction were compared by HPLC detection. Experimental results such as image 3 shown.

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Abstract

The invention discloses a preparation method and application of a poplar transformed product for improving enteritis. The poplar transformed product is prepared by hydrolyzing a poplar extract with beta-glucosidase. Wherein the raw material of the poplar extract is poplar sawdust and / or poplar leaves abandoned in poplar processing, and the beta-glucosidase is prepared through microbial fermentation or recombinant bacterium expression and purification. The preparation method provided by the invention has the characteristics of abundant raw material resources, low cost, simplicity, environmental protection, mild reaction, good anti-inflammatory activity of the extract and the like, and the in-vitro anti-inflammatory activity is respectively improved by 30.39% and 23.98% compared with the poplar sawdust extract and the poplar leaf extract which are obtained without enzyme hydrolysis. The extract prepared through the technology has the effect of improving animal colitis, and comprehensive utilization of poplar resources can be promoted.

Description

technical field [0001] The invention belongs to the technical field of natural substance extraction, and in particular relates to a preparation method and application of a poplar conversion product for improving enteritis. Background technique [0002] Poplar is widely distributed in eastern China and is one of the important tree species for afforestation and wood processing in my country. Poplar bark and branches and leaves are rich in plant secondary metabolites such as phenolic glycosides, flavonoids, organic acids, and tannins, which can play certain roles in anti-inflammatory, antibacterial, and anti-oxidative aspects. As a series of known glycosylated and esterified derivatives of salicyl alcohol, poplaretin has excellent biological activity. Small poplar bark has the effect of resisting Shigella, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and the bark of Populus quivering can also have good antibacterial effect on Botrytis cinerea, Penicilliu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/76A61P1/00A23K10/32A23K20/10A61K127/00A61K135/00
CPCA61K36/76A61P1/00A23K10/32A23K20/10A61K2236/19A61K2236/333A61K2236/35A61K2236/39Y02E50/10
Inventor 房仙颖王文杰赵林果
Owner NANJING FORESTRY UNIV
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