Small peptide with function of preventing and treating enteritis and application thereof

A technology for enteritis and function, applied in the application field of medicine, can solve the problem of lack of small peptides for treatment and prevention of enteritis, and achieve the effect of improving colon pathology and inflammation level, improving health level, and simple extraction and purification

Active Publication Date: 2021-10-01
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of highly efficient small peptides for treating and preventing enteritis in the prior art

Method used

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  • Small peptide with function of preventing and treating enteritis and application thereof
  • Small peptide with function of preventing and treating enteritis and application thereof
  • Small peptide with function of preventing and treating enteritis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Effect of small peptides of the present invention on the proliferation activity of normal colonic epithelial cells NCM460

[0023] Wheat germ protein is digested by simulating the gastrointestinal tract and separated by ultrafiltration to obtain several small peptides. After identification, the amino acid sequence of one of the small peptides is MDTSAPSPF (hereinafter referred to as the small peptide of the present invention, figure 1 ). The sequence of the small peptide of the present invention is shown in SEQ ID NO:1. The small peptide of the present invention was synthesized by Nanjing GenScript Biotechnology Co., Ltd. Through a lot of screening work, it is found that the small peptide of the present invention not only has good anti-inflammatory activity, but also can effectively alleviate the inflammatory injury of mouse colon.

[0024] The effect of the small peptide of the present invention on the proliferation activity of normal colonic epithelial cel...

Embodiment 2

[0027] Example 2 Effect of small peptides of the present invention on colitis model mice

[0028] 1) Modeling and administration

[0029] Six-week-old male C57BL / 6 mice were purchased from Puhe Biomedical Technology Co., Ltd. (Wuxi, China). Fifty C57BL / 6 mice were randomly divided into 5 groups, 10 in each group, which were normal group, model group, model + small peptide high-dose group, model + small peptide low-dose group, and positive control group. Except for the normal group, other groups adopted the following method to establish a colitis mouse model: the experimental mice freely drank 2.5% DSS (dextran sodium sulfate) aqueous solution for 7 days to establish a colitis mouse model.

[0030] During the establishment of the colitis mouse model, the mice in the model group were administered no other drugs. The model + small peptide high-dose group and the model + small peptide low-dose group were administered the colitis model drug DSS, and the model + small peptide high...

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PUM

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Abstract

The invention provides a small peptide with a function of preventing and treating enteritis and application thereof, and belongs to the technical field of biology. The amino acid sequence of the small peptide with the function of preventing and treating enteritis is shown as SEQ ID NO: 1, and the amino acid sequence is MDTSAPSPF. The small peptide can effectively prevent and treat enteritis related diseases. The small peptide is derived from wheat germs, and has the characteristics of safety, high efficiency and wide source. The small peptide is simple and convenient to extract and purify and has wide prospects in the fields of food, medicine and the like, so that the small peptide can be applied to preparation of medicines or functional foods for preventing and treating enteritis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a small peptide with anti-inflammatory activity and its application in the preparation of medicines for preventing and treating enteritis. Background technique [0002] Enteritis diseases are frequently-occurring and commonly encountered diseases, which seriously affect people's health. These diseases are stubborn and difficult to heal, have a long course of disease, and are prone to repeated attacks. Among them, colitis is more common in intestinal inflammatory diseases, which can be divided into two categories: acute phase and chronic phase. If patients in the acute stage are properly controlled, their symptoms can be relieved and finally cured. Otherwise, the inflammation will recur and eventually become chronic, increasing the risk of colon cancer. It is one of the most serious diseases of the gastrointestinal tract and is recognized by the World Health Organization. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P1/00
CPCC07K7/06A61P1/00A61K38/00Y02A50/30
Inventor 汪芳赖春燕沈新春陈元蓉
Owner NANJING UNIV OF FINANCE & ECONOMICS
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