Swine vesicular disease virus viral protein 1 (VP1) protein secretion expression recombinant plasmid and application thereof
A porcine vesicular disease virus and recombinant plasmid technology, applied in the field of protein expression, can solve the problems such as the inability of the recombinant protein to be secreted out of the cell, unfavorable for the purification of the recombinant protein, difficulty in protein purification, etc., so as to facilitate the extraction and purification, reduce the production cost, and improve the purification efficiency. Effect
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[0037] Example
[0038] 1. Materials and methods
[0039] 1.1 Strains and plasmids
[0040] Escherichia coli (E.coli) DH5α, DH10Bac (including Bacmid and Helper plasmids), and pFastBac1 plasmids are products of Invitrogen; pMD18-T plasmids are products of Dalian Bao Bioengineering Co., Ltd.; swine vesicular disease virus nucleic acid, insect cell Sf9 The passaged cells were provided by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau.
[0041] 1.2 Main reagents
[0042] Porcine vesicular disease virus positive and negative sera were provided by the Institute of Animal Health (IAH) in the United Kingdom; biotin-labeled anti-pig secondary antibodies were products of Abcom Company; SF-900Ⅱ medium and liposome Ⅱ were products of Invitrogen Company.
[0043] 1.3 Construction of recombinant transposition vector
[0044] 1.3.1 Construction of a vector with the addition of melittin signal peptide sequence
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