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Preparation method of scutellaria root flavone general aglycone extract

A technology of baicalin flavonoids and total aglycones, which is applied in the field of extraction technology of baicalin flavones and total aglycones, can solve the problems of incomplete enzymatic hydrolysis reaction, high production cost, incomplete hydrolysis of baicalin, etc., and achieves simple and convenient steps and excellent production process Feasible and easy to extract and purify

Inactive Publication Date: 2007-08-01
SICHUAN INST OF CHINESE MATERIA MEDICA
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AI Technical Summary

Problems solved by technology

Its disadvantages are: (1) under the condition of normal temperature (10~30 ℃), the hydrolysis of baicalin is not complete within 24 hours, and the utilization rate of medicinal material is low; Scutellaria baicalin total aglycone extract is obtained by column chromatography, and the cost of industrial production is high
Its disadvantages are: (1) the enzymatic hydrolysis reaction is incomplete under the condition of 41-46 ℃ of heat preservation for 4.1-4.8 hours, and the utilization rate of medicinal materials is low; It is convenient to recover ethanol for recycling, and generally do not use absolute ethanol to reflux to extract Chinese herbal medicines; in addition, this method still needs to dry the medicinal materials after enzymatic hydrolysis, and then use anhydrous ethanol to reflux for extraction, which increases the production process and energy consumption. Therefore, The extraction process has high cost and poor feasibility of industrial production

Method used

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  • Preparation method of scutellaria root flavone general aglycone extract
  • Preparation method of scutellaria root flavone general aglycone extract

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Take 10 kg of Scutellaria baicalensis and crush it through a 10-mesh sieve, add 60 liters of water, heat to 60°C and keep it warm for 10 hours; filter, discard the filtrate, extract the dregs after removing the filtrate with ethanol, and add 70% concentration of 80 liters of ethanol, reflux extraction twice, each time for 1 hour; filter, combine the ethanol extracts, recover the ethanol under reduced pressure until there is no alcohol smell, let it cool, and precipitate precipitates, filter the precipitates, and dry them to obtain the total aglycone extraction of Scutellaria baicalensis 1.7 kilograms of material, wherein baicalein content is 35.4%, and total flavonoid content is 75.1%.

Embodiment 2

[0045] Take 10 kg of Scutellaria baicalensis and crush it through a 15-mesh sieve, add 50 liters of water, heat to 65°C and keep it warm for 8 hours; filter, discard the filtrate, extract the dregs after removing the filtrate with ethanol, and add 85% concentration of 60 liters of ethanol, reflux extraction twice, each time 2.5 hours; filter, combine the ethanol extracts, recover the ethanol under reduced pressure until there is no alcohol smell, let it cool, and precipitate precipitates, filter the precipitates, dry, and obtain the total aglycone extraction of Scutellaria baicalensis 1.6 kg of material, wherein the baicalein content is 35.8%, and the total flavonoid content is 76.0%.

Embodiment 3

[0047] Take 10 kg of Scutellaria baicalensis and crush it through a 10-mesh sieve, add 80 liters of water, heat to 55°C and keep it warm for 15 hours; filter, discard the filtrate, extract the dregs after removing the filtrate with ethanol, and add 90% concentration of 50 liters of ethanol, reflux extraction 3 times, 0.5 hours each time; filter, combine ethanol extracts, recover ethanol under reduced pressure until there is no alcohol smell, let it cool, and precipitate precipitates, filter and dry the precipitates to obtain the total aglycone extraction of Scutellaria baicalensis 1.5 kilograms of material, wherein baicalein content is 36.5%, and total flavonoid content is 76.3%.

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Abstract

The invention provides a method for preparing baikal skullcap root chromocor aglycone extract, comprising following steps: disintegrating baikal skullcap root, adding water with amount being 4- 10 times of baikal skullcap root, heating to 40- 70 Deg. C and thermal insulating for 5- 24 hours, enzymolyzing, filtering after reaction, throwing away filtering liquid, extracting dregs of a decoction with ethanol, recovering ethanol, depositing, filtering and drying, getting baikal skullcap root chromocor aglycone extract. The reaction time can be adjusted along with enzymolysis temperature to get optimum enzymolysis effect and increase medicinal materials availability ratio; the water soluble foreign substance can be remove buy filtering hydrolysate, which is convenient for following chromocor aglycone extraction. The invention is characterized by high productivity, simple operation, low cost and suitability for industrial production.

Description

technical field [0001] The invention relates to a preparation method of active ingredients of traditional Chinese medicines, in particular to an extraction process of total flavone aglycones of scutellaria baicalensis (or total aglycones of scutellaria baicalensis, total flavone aglycones of scutellaria baicalensis). Background technique [0002] Scutellaria baicalensis Georgi is a commonly used traditional Chinese medicine recorded in the Chinese Pharmacopoeia. It has the effects of clearing away heat, purging fire and detoxifying. Studies have shown that the main active ingredients of Scutellaria baicalensis are flavonoids such as baicalin, wogonin, baicalein (baicalein) and wogonin, and the effect of baicalein is stronger than that of baicalin (Hu Shilin et al.: "Chinese Journal of Pharmaceutical Sciences" 2001, 36 (11): 728), and is the composition with the strongest antioxidant effect among the above four flavonoids. A comparative study on the pharmacological effects o...

Claims

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Application Information

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IPC IPC(8): C12P17/06C07D311/30
Inventor 易进海刘云华陈燕黄志芳刘玉红
Owner SICHUAN INST OF CHINESE MATERIA MEDICA
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