Glucomannan enzyme preparation method

A technology of glycanase and glucomannan, which is applied in the field of glucomannanase preparation, can solve the problems of limited access to glucomannan oligosaccharides, and achieve the effects of easy extraction and purification, high enzyme activity and simple process

Inactive Publication Date: 2008-05-21
GUANGDONG INST OF MICROORGANISM +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] A preparation method of glucomannanase has been disclosed in Chinese patent ZL200510034300.2, but the glucomannanase obtained by this method can only degrade konjac powder, which limits the access to glucomannan oligosaccharides

Method used

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  • Glucomannan enzyme preparation method

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Experimental program
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Effect test

Embodiment 1

[0020] Mix 6 g of bagasse, 1 g of bran, 0.5 g of konjac fine powder, 1.5 g of agar, 2 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 0.05 g of magnesium sulfate, and 100 g of water to make a test tube slant culture medium and sterilize it. Trichodermaviride CGMCC3.2942 bred by ultraviolet mutagenesis was inoculated into the above slant culture medium, cultured at 28° C. for 4 days, and 150 mL of spore suspension was obtained.

[0021] Bagasse 73g (73%), bran 15g (15%), yeast cell wall 10g (10%), konjac powder 2g (2%) are mixed to obtain a solid medium, ammonium sulfate 1.1g, potassium dihydrogen phosphate 0.11g , 0.055g of magnesium sulfate and 218.735g of water are mixed to obtain 220g of nutrient salt water. Take 100 g of the solid koji culture medium obtained by mixing the solid medium and nutrient saline (mass ratio 1:2.2), put it in a 1000 mL Erlenmeyer flask, sterilize it, add 8 mL of spore suspension, shake it well, and culture it at a constant temperatur...

Embodiment 2

[0027] Mix 6 g of bagasse, 1 g of bran, 0.5 g of konjac fine powder, 1.5 g of agar, 1 g of yeast cell wall, 2 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 0.05 g of magnesium sulfate, and 100 g of water to make a test tube slant medium, bacteria. Trichoderma viride CGMCC3.2942 selected by ultraviolet mutagenesis was inoculated into the above-mentioned slant medium, cultured at 28°C for 4 days, and 150 mL of spore suspension was obtained.

[0028] Bagasse 65g (65%), bran 20g (20%), yeast cell wall 12g (12%), konjac powder 3.0g (3%) were mixed to obtain a solid medium, ammonium sulfate 1.035g, potassium dihydrogen phosphate 0.1035 g, magnesium sulfate 0.046g, mixed with water 228.8155g to obtain 230g of nutrient salt water. Take 100 g of the solid koji culture medium obtained by mixing the solid medium and nutrient saline (mass ratio 1:2.3), put it in a 1000 mL Erlenmeyer flask, sterilize it, insert 15 mL of the spore suspension, shake it up and culture it at 3...

Embodiment 3

[0035] Bagasse 6g, bran 1g, konjac fine powder 0.5g, agar 1.5g, yeast cell wall 1g, ammonium sulfate 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.05g, mix with water 100g, make test tube slant culture medium, kill bacteria. Trichoderma viride (Trichoderma viride) CGMCC3.2942 bred by ultraviolet mutagenesis was inoculated into the slant medium, cultured at 28° C. for 4 days, and 150 mL of spore suspension was obtained.

[0036]Bagasse 66g (66%), bran 15g (15%), yeast cell wall 15g (15%), konjac powder 4g (4%) are mixed to obtain a solid medium, ammonium sulfate 1.38g, potassium dihydrogen phosphate 0.138g , magnesium sulfate 0.069g, mixed with water 228.413g to obtain 230g of nutrient salt water. Get 100 g of the solid koji culture medium obtained by mixing the solid medium and nutrient saline (mass ratio 1:2.3), put it in a 1000mL Erlenmeyer flask, sterilize it, insert 8mL of the spore suspension, shake it up and culture it at a constant temperature at 27°C for ...

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Abstract

The invention relates to a preparation method of glucomannanase, which is characterized in that Trichoderma viride (Trichoderma viride) CGMCC3.2942 selected and bred by ultraviolet mutagenesis is used as an enzyme-producing strain, and the koji is cultivated in a solid koji culture medium. The solid koji medium is composed of solid medium and nutrient saline with a mass ratio of 1:2.2-2.3, wherein the solid medium is composed of bagasse 65%-73%, bran 15%-20%, yeast cell wall 10%-15% %, 2.0% to 4.0% of konjac powder, and then through the steps of enzyme liquid extraction, enzyme sludge precipitation, purification, etc., to obtain glucomannanase. The invention has the advantages of simple process, low equipment investment, low cost, no pollution, easy product extraction and purification, and the obtained glucomannanase can efficiently degrade plant-like glucomannan to obtain glucomannan oligosaccharides, and can more effectively degrade Yeast-like cell walls obtain yeast glucomannan-oligosaccharides.

Description

technical field [0001] The present invention relates to a preparation method of glucomannanase, in particular to a preparation method of glucomannanase capable of degrading plant-like glucomannan to obtain glucomannan oligosaccharides and more effectively degrading yeast cell walls . Background technique [0002] Glucomannan oligosaccharide is a kind of oligosaccharide with great application value. It not only widely exists in konjac and other plants, but also is the main component of yeast cell wall. Glucomannan oligosaccharides can significantly promote the proliferation of probiotics in the human intestinal tract, reduce the production of toxic fermentation products and harmful bacteria, and are good immune enhancers for humans and animals. They can be used as human functional food additives or animal feed additives to improve The level of human health and the feeding value of farmed animals are of great significance, and the use of oligosaccharide derivatives as human m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24C12N1/14C12R1/885
Inventor 吴月嫦许国焕江永明孟朋邹德良刘刚
Owner GUANGDONG INST OF MICROORGANISM
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