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Extraction method of Araceae polysaccharide

An extraction method and technology of radix, applied in the directions of cleaning methods using liquid, chemical instruments and methods, cleaning methods and utensils, etc., can solve the problems of low extraction efficiency, high content of polysaccharide impurities in radix, etc., and achieves simple operation and loss. small effect

Inactive Publication Date: 2020-05-19
西藏天虹科技股份有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polysaccharide from the pothos has anti-oxidation and moisturizing effects. The existing extraction method of the polysaccharide from the pothos is usually directly extracted with water, and then purified with an ethanol solution. The obtained polysaccharide contains many impurities and the extraction efficiency is low.

Method used

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  • Extraction method of Araceae polysaccharide
  • Extraction method of Araceae polysaccharide
  • Extraction method of Araceae polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A method for extracting polysaccharides from green dill flower, comprising the following steps:

[0025] S1. Take the pothos flower, wash and crush it to obtain a crushed product, and soak the crushed product in a sodium chloride solution with a mass fraction of 20% for 2 hours to obtain the first mixed solution, wherein the pothos flower and 20% sodium chloride solution with a mass fraction of The weight ratio of sodium solution is 1:3;

[0026] S2. The first mixed solution was frozen at -20°C for 2 hours, then ultrasonically treated at 20°C and 25 kHz for 40 minutes, heated to 85°C for 50 minutes, and then filtered to obtain a filtrate;

[0027] S3. Place the filtrate in a dialysis bag with a molecular weight cut-off of 3500 Da, and place the sealed dialysis bag in a polyethylene glycol solution with a mass fraction of 80% for 60 minutes, then take out the dialysis bag, and pour out the dialysis bag The liquid in the mixture was obtained to obtain the second mixed so...

Embodiment 2

[0030] A method for extracting polysaccharides from green dill flower, comprising the following steps:

[0031] S1. Wash and pulverize the pothos flower to obtain a pulverized product, soak the pulverized product in a sodium chloride solution with a mass fraction of 20% for 4 hours to obtain the first mixed solution, wherein the pothos flower and 20% sodium chloride solution with a mass fraction of The weight ratio of sodium solution is 1:3;

[0032] S2. Freeze the first mixed solution at -20°C for 4 hours, then place it at 40°C and 40kHz for 80 minutes of ultrasonic treatment, then heat it to 100°C and keep it for 80 minutes, then filter to obtain the filtrate;

[0033] S3. Place the filtrate in a dialysis bag with a molecular weight cut-off of 3500 Da, and place the sealed dialysis bag in a polyethylene glycol solution with a mass fraction of 80% for 80 minutes, then take out the dialysis bag, and pour out the dialysis bag The liquid in the bag is obtained as the second mix...

Embodiment 3

[0036] A method for extracting polysaccharides from green dill flower, comprising the following steps:

[0037] S1. Wash and pulverize the pothos flower to obtain a pulverized product, soak the pulverized product in a sodium chloride solution with a mass fraction of 20% for 3 hours to obtain the first mixed solution, wherein the pothos flower and 20% sodium chloride solution with a mass fraction of The weight ratio of sodium solution is 1:3;

[0038]S2. Freeze the first mixed solution at -20°C for 3 hours, then place it at 30°C and 32kHz for 60 minutes of ultrasonic treatment, then heat it to 90°C and keep it for 65 minutes, then filter to obtain the filtrate;

[0039] S3. Place the filtrate in a dialysis bag with a molecular weight cut-off of 3500 Da, and place the sealed dialysis bag in a polyethylene glycol solution with a mass fraction of 80% for 70 minutes, then take out the dialysis bag, and pour out the dialysis bag The liquid in the mixture was obtained to obtain the ...

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Abstract

The invention discloses an extraction method of Araceae polysaccharide, which comprises the following steps: soaking Araceae in a sodium chloride solution, carrying out ultrasonic treatment, treatingin a dialysis bag, pouring out liquid in the dialysis bag, carrying out distillation treatment, adding an ethanol solution, and carrying out repeated purification to obtain the Araceae polysaccharide.The operation method is simple, and cell walls of Araceae are broken by utilizing a sodium chloride solution, so that contents in cells are dissolved out; rapidly concentrating of the filtrate is carried out by using the dialysis bag to obtain a liquid containing less impurities; purifying is carried out for multiple times by utilizing an ethanol solution to obtain the high-purity Araceae polysaccharide.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction. More specifically, the present invention relates to a method for extracting polysaccharides from pothos. Background technique [0002] Green radish, also known as golden kudzu, vine taro, stone orange, and horseshoe gold, is a plant of the Araceae family and is a unique and precious plant in Tibet. Produced in the cold region of Tibet, in the Himalayas, it blooms from July to August every year, and the flowering period is about 30 days. It is slightly cold in nature. The polysaccharide from the pothos has anti-oxidation and moisturizing effects. The existing method for extracting the polysaccharide from the pothos is generally to directly extract it with water, and then purify it with an ethanol solution. The obtained polysaccharide contains many impurities and the extraction efficiency is low. Contents of the invention [0003] It is an object of the present invention to solve at lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00B08B3/08B04B13/00
CPCB04B13/00B08B3/08C08B37/0003
Inventor 张春颖
Owner 西藏天虹科技股份有限责任公司
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