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Culture medium for promoting synthesis of antibacterial polypeptide gramicidin and culture method thereof

A gramicidin and antibacterial peptide technology, applied in the biological field, can solve problems such as complex culture methods, achieve the effects of improving synthesis, promoting extraction, purification, development and application, and promoting expression

Pending Publication Date: 2019-09-24
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the culture medium and culture conditions that promote the synthesis of the polypeptide antibiotic linear gramicidin (linear Gramicidin) in Bacillus brevus, and there are deficiencies such as complex culture methods. It is urgent to screen suitable culture media and improve culture methods to improve Its synthetic yield promotes its extraction, purification, development and application

Method used

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  • Culture medium for promoting synthesis of antibacterial polypeptide gramicidin and culture method thereof
  • Culture medium for promoting synthesis of antibacterial polypeptide gramicidin and culture method thereof
  • Culture medium for promoting synthesis of antibacterial polypeptide gramicidin and culture method thereof

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Effect test

Embodiment 1

[0033] The present embodiment prepares 1L SAM liquid culture medium, preparation method is as follows:

[0034] Step 1, weigh 15g of sucrose, 2g of asparagine, K 2 HPO 4 Dissolve 1g in 800ml double-distilled water, mix and dissolve completely, shake well, and prepare solution A;

[0035] Step 2, take the MgSO of required weight portion 4 ·7H 2 O 1g, dissolved in 200ml double-distilled water, dissolved completely, shaken well, and prepared solution B;

[0036] Step 3: Autoclave solution A and solution B at 121°C for 25 minutes respectively; after the sterilization, cool down to room temperature naturally, mix the two evenly, and adjust the pH value to 7.0 to obtain the finished product SAM liquid medium.

Embodiment 2

[0038] The present embodiment prepares 1L SAM liquid culture medium, preparation method is as follows:

[0039] Step 1. Weigh 25g of glycerol, 3g of proline, K 2 HPO 4 Dissolve 1g in 800ml double-distilled water, mix and dissolve completely, shake well to prepare solution A;

[0040] Step 2, take the MgSO of required weight portion 4 ·7H 2 O 2g, dissolved in 200ml double-distilled water, dissolved completely, shaken well, prepared solution B;

[0041] Step 3: Autoclave solution A and solution B at 123°C for 22 minutes respectively; after sterilization, cool down to room temperature naturally, mix the two evenly, and adjust the pH value to 7.0 to obtain the finished product SAM liquid medium.

Embodiment 3

[0043] The present embodiment prepares 1L SAM liquid culture medium, preparation method is as follows:

[0044] Step 1, weigh 20g of sucrose, 2g of proline, K 2 HPO 4 Dissolve 2g in 800ml double-distilled water, mix and dissolve completely, shake well to prepare solution A;

[0045] Step 2, take the MgSO of required weight portion 4 ·7H 2 O 1g, dissolved in 200ml double-distilled water, dissolved completely, shaken well, and prepared solution B;

[0046] Step 3: Autoclave solution A and solution B at 118°C for 30 minutes respectively; after the sterilization, cool down to room temperature naturally, mix the two evenly, and adjust the pH value to 7.0 to obtain the finished product SAM liquid medium.

[0047] The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any form. Any modifications and equivalents to the above embodiments that do not deviate from the content of the technical solution of ...

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Abstract

The invention relates to the technical field of biology, in particular to a culture medium for promoting the synthesis of antibacterial polypeptide gramicidin and a culture method thereof. The culture medium comprises the following raw materials in parts by weight: 15-25 parts of carbon source, 2-3 parts of nitrogen source, 1-3 parts of K2HPO4 and 1-3 parts of MgSO4.7H2O. In the aspect of promoting the synthesis of the linear short-stem peptide in the bacillus brevis, compared with conventional NA and NB culture mediums, the finished product of SAM liquid culture medium can greatly promote the expression of the linear gramicidin synthetase coding gene, and the expression amount is about 7-9 times of that of the conventional NB liquid culture medium, so that the synthesis amount of the linear gramicidin is greatly improved, and for specific performance, the inhibition zone on the pathogenic bacteria of Candida albicans is increased. The linear gramicidin cultured by the culture method not only can meet the research requirements, but also can promote the extraction, purification, development and application of the linear gramicidin.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a medium for promoting the synthesis of antibacterial polypeptide gramicidin and a culture method thereof. Background technique [0002] Brevibacillus brevis in the genus Brevibacillus can synthesize multiple polypeptide antibiotics such as linear Gramicidin and cyclopeptidetycocidine. Due to the high antibacterial activity, wide antibacterial spectrum, many types, wide range of options, and the target strains are not easy to produce resistance mutations and other reasons, polypeptide antibiotics have broad application prospects in the pharmaceutical industry. [0003] Conventionally, nutrient agar medium (NAmedium) is used for solid culture of Bacillus brevis, while nutrient broth medium (NBmedium) is used for liquid culture. The yields of linear Gramicidin and cyclopeptidetycocidine in conventional NA and NB medium were low and unstable. [0004] Numerous studies have found that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/01
CPCC12P21/02C07K7/66
Inventor 刘红美盛淼淼贾化可
Owner GUIZHOU MEDICAL UNIV
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