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72 results about "Daphne giraldii" patented technology

Daphne giraldii is a shrub, of the family Thymelaeaceae. It is deciduous, and is found in regions of China.

Slow-release patch with daphne giraldii bark extract, and preparation method thereof

The invention belongs to the technical field of medicament, and discloses a percutaneous slow-release patch with a daphne giraldii bark extract, and a reparation method thereof. The patch with the daphne giraldii bark extract comprises a back lining layer, a drug reservoir and an antisticking layer, wherein the drug reservoir consists of the daphne giraldii bark extract, pressure sensitive adhesive and a percutaneous absorption accelerator; plasticizer, tackifier and antioxidant and the like can be added if needed; the selected pressure sensitive adhesive is silicone pressure sensitive adhesive, isobutylene pressure sensitive adhesive or acrylate pressure sensitive adhesive, and can select one or more compounds therein. The percutaneous patch has the advantages that the percutaneous patch can prevent oral administration from causing liver first-pass effect and irritating gastrointestinal tracts, overcomes deficiency that ointment, plaster, liniment, cream and other preparations are uncertain in dosage, easy to pollute clothes and the like, is constant in drug-releasing speed, ensures lasting steady therapeutic effect, and is convenient to use, good in flexibility and suitable in adhesion. The 24-hour accumulated permeation amount of daphnetin in a daphne giraldii bark patch prepared by the method is obviously higher than that of the commercially available daphne giraldii bark plaster.
Owner:SHENYANG PHARMA UNIVERSITY

Bone fracture setting medicine powder for treating traumatic injury

The invention relates to a bone connecting powder for treating traumatic injury, which is made from 5 to 15 parts of daphne giraldii nitsch, 5 to 10 parts of saffron, 5 to 15 parts of notoginseng, 5 to 15 parts of chuanxiong rhizome, 5 to 10 parts of frankincense, 5 to 10 parts of myrrh, 6 to 20 parts of dragons blood, 5 to 15 parts of sappan wood, 10 to 20 parts of angelica, 5 to 15 parts of pyrite, 5 to 10 parts of ground beetle, 3 to 8 parts of hippocampus, 5 to 10 parts of rhizoma drynariae, 3 to 8 parts of pilose deer horn, and 1 to 3 parts of musk in definite weight proportion The fifteen bulk drugs are jointly ground into fine powder, and processed to particles with a fineness of 120 meshes, and then mixed evenly and made into pulvis. The powder of the invention has the functions of promoting blood circulation to dispel blood stasis, reducing swelling and alleviating pain, and promoting reunion of the fractured bones, and has apparent analgesic effect on soft tissue fracture, comminuted fracture and old injuries, the early-stage and medium-stage osteonecrosis, compression fracture of lumbar vertebra, bone lesion, lumbar disc protrusion and the like in the bone injury treatment. The powder of the invention has the advantages of quick effect-taking, good curative effect, short treatment course, convenient use, being safe and reliable, and no side effect. The clinical effective rate of the powder for more than 1200 cases is 96 percent.
Owner:包雁仲

Anti-tumor traditional Chinese medicine composition and preparation method and application thereof

The invention discloses an anti-tumor Chinese medicine composition and a preparation method and application thereof. The Chinese medicine composition is prepared with the following raw materials in parts by weight: 10-100 parts of ligusticum wallichii, 10-200 parts of corydalis amabilis, 1-50 part(s) of prepared monkshood, 10-300 parts of daphne giraldii nitsche, 10-100 parts of gentiana macrophylla and 1-50 part(s) of venenum bufonis. The traditional Chinese medicine composition provided by the invention is prepared according to the formula of the traditional Chinese medicine based on the theory of the traditional Chinese medicine and the theory of syndrome differentiation and treatment, and the results of in vivo and in vitro experiments show that the traditional Chinese medicine composition provided by the invention has the functions of inhibiting the growth of the tumor cell and promoting the apoptosis of the tumor cell, and has a strong function of killing a variety of tumor cells. Therefore, the scope of application of the traditional Chinese medicine composition is wide. Moreover, the extract of the traditional Chinese medicine composition provided by the invention can be prepared as various preparations, can be taken conveniently in clinical treatment and has no toxic and side effect in the long-term use.
Owner:雷允上药业集团有限公司

Preparation method and content measurement method of Daphne giraldii Nitsche total coumarin extract

The invention provides a preparation method and a content measurement method of a Daphne giraldii Nitsche total coumarin extract. The preparation method comprises: performing extraction by using a Daphne giraldii Nitsche herbal medicament as a raw material and water and/or C1 to C5 lower alcohol as a solvent to obtain an extract; mixing the extract with a solid diluent, adding an organic solvent for extraction, recovering the solvent to obtain a solid extract, or adding the extract into water for suspension, adding the organic solvent for extraction and receovering the solvent to obtain the solid extract; adding the solid extract into water for suspension, performing chromatography by macroporous adsorption resin in a way of eluting by using water or the aqueous solution of the C1 to C5 lower alcohol as a flowing phase to remove impurities first, then using the aqueous solution of the lower alcohol for elution and finally collecting eluent; reclaiming the solution from a previous half part of eluent to obtain a solid which is part of the Daphne giraldii Nitsche total coumarin extract; and reclaiming the solution from the later half part of eluent to obtain a solid, adding water into the solid for suspension, extracting the solid with an organic solvent and recovering an organic layer to obtain the solid which is the other part of the Daphne giraldii Nitsche total coumarin extract.
Owner:GUANGZHOU LIXIN PHARM CO LTD

Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof

The invention relates to an effective component group of daphne giraldii nitsche for resisting rheumatoid arthritis with active ingredients of total coumarine and total saponin. The effective component group is prepared by the following methods according to a certain weight ratio: the daphne giraldii nitsche medicinal materials are ground into coarse powder which is first extracted by alcohol, and then the alcohol is recovered from the alcohol extraction liquid, water is added for scattering the alcohol extraction liquid, two organic solvents are used in sequence for extraction, recovery and concentration to first obtain a extractive matter (diterpenoid ingredient) and then obtain b extractive matter (total coumarine ingredient); a extractive matter(diterpenoid ingredient)is abandoned, macropore adsorption resin column is applied to liquid medicine obtained from the extraction of the b extractive matter, the liquid medicine is eluted by ethanol, the ethanol is recovered and concentrated to obtain c extractive matter (total saponin ingredient); finally, effective component group of daphne giraldii nitsche for resisting rheumatoid arthritis is obtained by mixing the total coumarine ingredient and the total saponin ingredient according to a certain weight ratio. The effective component group of daphne giraldii nitsche features simple preparation process, easy operation and low cost, in addition, no high temperature equipment or high voltage equipment is needed, the poisonous diterpenoid ingredient is removed, thus reducing toxicity and improving safety. The result of pharmacological tests indicates that the effective component group of the invention has significant effect on curing the rheumatoid arthritis and is superior to single ingredient total coumarin.
Owner:NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE

Effective site group of daphne giraldii nitsche leaf, preparation method, medicinal composition and application thereof

The invention relates to an effective site group of daphne giraldii nitsche leaf, a preparation method thereof, a medicinal composition containing the effective site group of the daphne giraldii nitsche leaf and application of the effective site group of the daphne giraldii nitsche leaf to the preparation of medicaments for treating diseases such as traumatic injury, arthritis and the like, and belongs to the technical field of pharmaceutics of traditional Chinese medicine. The effective site group of the daphne giraldii nitsche leaf is high in active ingredients and obvious in medicinal effect, medicinal sites do not damage vegetations and environment, the extraction process is easy and convenient, and the operability is high.
Owner:中国人民解放军第四五一医院

Mesenchymal stem cell derived cartilage cell preparation and preparation method thereof

The invention provides a mesenchymal stem cell derived cartilage cell preparation and a preparation method thereof; the mesenchymal stem cell derived cartilage cell preparation comprises a first injection, the first injection is mainly made from a raw material, auxiliary material I and cartilage cells along with injection water, the raw material is mainly made from, by weight, 10-60 parts of Daphne giraldii extract, 10-50 parts of Salvia miltiorrhiza extract, and 10-40 parts of cibot rhizome extract. The first injection provided herein is directly applicable to filling materials for facial cosmetic surgery; by injecting the cartilage cells, it is possible to reconstruct cartilage tissues and effectively decreases the rejection rate; the suitable growth environment lays the basis for the differentiation and proliferation of cartilage cells, graft survival rate of cartilage cells is increased, a patient feels less pain during rhinoplasty or craniofacial repair, and the surgical period is shortened; the mesenchymal stem cell derived cartilage cell preparation is widely applicable to the surgeries, such as craniofacial injury, cartilage scaffold reconstruction, form defect repair, laryngoplasty in otorhinolaryngology, and rhinoplasty.
Owner:华夏(青岛)生物科技有限公司

Plaster for treating osteopathy

The invention discloses a plaster for treating osteopathy. The plaster comprises the following raw materials in parts by weight: nux vomica, monkshood, radix aconiti agrestis, frankincense, myrrh, lumbricus, whole worm, notopterygium root, radix angelicae pubescentis, pawpaw, teasel root, cocklebur fruit, mulberry twig, gentian, agkistrodon, zaocys dhumnade, rhizoma typhonii, homalomena occulta, cortex acanthopanacis, photinia stems, salvia miltiorrhiza, ligusticum wallichii, radix achyranthis bidentatae, daemonorops draco, cutch, Chinese parsnip roots, pangolin, hematoxylon, akebiaquinata, schizophragma integrifolium, desmodium, concha haliotidis, gastrodia elata, pipewort, lycopodium clavatum, daphne giraldii nitsche, garden balsam stem, liquorice, cynomorium songaricum, ground beetle, castor bean, radix clematidis, herba epimedii, cistanche, gecko, sealwort, polygonum multiflorum and eucommia ulmoides. Various medicines are used together and jointly have the effects of diminishing inflammation to relieve pain, promoting blood circulation to remove blood stasis, stimulating the menstrual flow, smoothing acupuncture point, dispelling wind and eliminating dampness, dispelling coldness, softening hard mass and dissipating blood stasis, supplementing bone plasma, strengthening muscles and bones, activating blood, eliminating swelling and drawing out toxin. The plaster has an excellent effect for treating osteopathy.
Owner:穆林涛

Snow lotus herb wine

The invention discloses snow lotus herb wine and a preparation method thereof. The snow lotus herb wine is prepared from the following raw materials in parts by weight: 80-150 parts of snow lotus herb, 50-100 parts of Trametes orentalis, 15-25 parts of Corn Cervi Pantotrichum, 25-45 parts of phoenix tree leaf, 20-30 parts of rhizoma cibotii, 45-65 parts of angelica sinensis, 30-50 parts of zaocys dhumnade, 40-70 parts of red flower, 15-25 parts of root of common peony, 20-40 parts of the root of bidentate achyranthes, 40-70 parts of lucid ganoderma, 25-45 parts of Loropetalum chinense root, 40-70 parts of Rhodiola rosea, 30-50 parts of wintergreen, 45-65 parts of Daphne giraldii, 40-70 parts of lycium barbarum, 30-50 parts of Chinese waxgourd stem, 20-30 parts of garden balsam stem, 15-25 parts of rhizoma atractylodis, 10-15 parts of Stachys sieboldii Miq. and 4500-5500 parts of white spirits. The snow lotus herb wine disclosed by the invention has the effects of dispelling wind, eliminating dampness, clearing and activating the channels and collaterals, promoting blood circulation to remove blood stasis, relieving swelling and pain, strengthening tendons and bones and tonifying middle-Jiao and Qi, has obvious curative effects on rheumatoid arthritis, rheumatoid arthritis, scapulohumeral periarthritis, senile lumbocrural pain and the like, can take curative effect quickly, is wide in market prospect and does not have any toxic or side effect.
Owner:HEFEI YAOSHIYUAN AGRI TECH

Medicament for treating periodontitis and preparation method of medicament

The invention discloses a medicament for treating periodontitis, which is prepared from the following main raw materials in parts by weight: 9-16 parts of rhizoma alismatis, 7-14 parts of acanthopanax, 10-15 parts of hairy holly leaf, 8-16 parts of commelina diffusa, 6-13 parts of Chinese pear-leaved crabapple, 9-14 parts of populus alba leaf, 8-13 parts of orchis, 6-12 parts of rose hip, 7-13 parts of oblongleaf garcinia seed oil, 9-12 parts of spinach beet, 10-16 parts of dodder, 7-15 parts of Chinese usnea, 3-6 parts of daphne giraldii, 10-14 parts of dandelion and 8-12 parts of sheareria nana. The medicament disclosed by the invention has the effects of clearing away heat and purging pathogenic fire, resisting bacteria and diminishing inflammation as well as eliminating swelling and healing sores, is capable of quickly reducing an inflammatory surface and alleviating pain, has a good effect on treating periodontitis, and is free of toxic or side effect.
Owner:岳源

Method for increasing germination rate of daphne giraldii nitsche seeds

The invention discloses a method for increasing the germination rate of daphne giraldii nitsche seeds, and belongs to the fields of quality determination and cultivation of Chinese medicinal material seeds. The method comprises the following steps: (1) seed pretreatment: peeling daphne giraldii nitsche seeds, soaking the peeled daphne giraldii nitsche seeds into distilled water for 12-36 hours, and removing seeds floating on the water surface while leaving seeds submerged under water to obtain peeled seeds; (2) seed disinfection: soaking the peeled seeds into disinfectant for 10-40 minutes for disinfecting, and flushing with distilled water; (3) seed cultivation: placing the disinfected seeds into a tray loaded with a substrate for cultivating, and watering during cultivating; and (4) germination acceleration: placing the tray in which the seeds are planted into a manual climatic box to accelerate germination. The method for increasing the germination rate of the daphne giraldii nitsche seeds is simple, convenient and practicable, and has high repeatability, a good effect and high working efficiency. By using the method, the mildewing rate of the daphne giraldii nitsche seeds can be lowered, and the germination rate of the daphne giraldii nitsche seeds is increased effectively. The mildewing rate of the daphne giraldii nitsche seeds is 16.23 percent, and the germination rate is 83.77 percent.
Owner:甘肃凯源生物技术开发中心有限责任公司

Sterilization and disinfection type coupling agent and method for preparing same

The invention belongs to the technical field of medical coupling agents, and particularly relates to a sterilization and disinfection type coupling agent and a method for preparing the same. Active ingredients in the sterilization and disinfection type coupling agent are made of, by weight, 0.15-0.25 part of hydroxypropyl celluloses, 0.7-1.0 part of carbomer, 4-15 parts of propylene glycol, 82-95 parts of deionized water and 0.03-0.14 part of fungicides. The fungicides are made of, by weight, 20-30 parts of bigleaf fig bark, 15-25 parts of rhizoma anemarrhenae, 10-20 parts of fructus kochiae, 40-60 parts of bidens tripartita, 25-45 parts of aloe, 12-36 parts of sedum spectabile boreau, 10-16 parts of herba portulacae, 30-40 parts of ocimum basilicum, 15-35 parts of symplocos paniculata, 8-16 parts of herba andrographis, 16-26 parts of daphne giraldii nitsche, 20-28 parts of cactaceae, 30-40 parts of herba menthae, 14-28 parts of astragalus sinicus, 11-22 parts of cortex hibisci, 14-36 parts of rauvolfia verticillata, 16-30 parts of flos lonicerae and 15-30 parts of laminaria japonica. The sterilization and disinfection type coupling agent and the method have the advantages that the sterilization and disinfection type coupling agent can be used safely and comfortably, allergy reaction or cross infection can be prevented, and images are high in definition.
Owner:山东九尔医药生物科技有限公司

External liquid plaster for treating bone-arthrosis pain and preparation method of external liquid plaster

The invention discloses an external liquid plaster for treating bone-arthrosis pain. The external liquid plaster is prepared from the following raw materials: aconitum szechnyianum gay, aconitum taipeicum hand-mazz, fringed iris herb, Chinese umbrellaleaf rhizome and roots, arisacma consanguineum schott, raw pinellia, radix aconiti kusenzoffii, raw kusnezoff monkshood roots, raw momordica cochinchinensis kernel, garden balsam stems, Chinese azalea fruit, olibanum, myrrh, corsiopsis chinensis flowers and roots, radix clematidis, unprocessed semen strychni, centipede, vinegar-processed native copper, grub, acutangular anisodus roots, dimethyl sulfoxide, prednisone sheets, daphne giraldii nitsche liquid, borneol, medical azone, medical oleic acid, medical propylene glycol, essential balm and voltaren tablets. The invention further discloses a preparation method of the external liquid plaster for treating bone-arthrosis pain. The method comprises the steps of extracting, ethanol removal, dissolving, blending and filling. By using the characteristic of medicinal advantages of Chinese herbal medicines and a modern preparation, the external liquid plaster has the characteristics of being fast in acting on pain caused by osteoarthritis and not easy to rebound.
Owner:孙广荣

Traditional Chinese medicine preparation for treating prosopospasm

The invention discloses a traditional Chinese medicine preparation for treating prosopospasm. The preparation is prepared from the following medicinal materials in parts by weight: 15-25 parts of ligusticum wallichii, 5-12 parts of anaphalis lactea, 3-7 parts of pulvis fellis suis, 1-4 parts of trichosanthes kirilowii maxim, 5-12 parts of rabbit hearts, 3-9 parts of cornu cervi degelatinatum, 3-12 parts of polyporus umbellatus, 12-25 parts of radix phytolaccae, 5-13 parts of daphne giraldii nitsche, 3-9 parts of semen sophorae, 5-15 parts of ellpticleaf spurgentian root, 10-20 parts of rhizoma dioscoreae septemlobae, 12-24 parts of radices stellariae dichotomae and 5-10 parts of herba siegesbeckiae. The traditional Chinese medicine preparation is convenient to use, has good absorption effect and no adverse, toxic or side effect, and is simple in preparation.
Owner:宁贻伟

Acupoint injection medicine for treating rheumatic arthralgia, preparation method and application

The invention discloses an acupoint injection medicine for treating rheumatic arthralgia, a preparation method and an application, which relates to the medicine technical field of rheumatic arthralgia treatment. The invention provides the medicine for treating rheumatic arthralgia with the advantages of safe and effective performance, and the preparation method and the application. The acupoint injection medicine for treating rheumatic arthralgia comprises the following raw materials by volume ratio: 2-3 parts of Daphne giraldii Nitsche injection, 2-3 parts of gen-seng injection, 2-3 parts of saussurea involucrata injection, 2-3 parts of clematis root injection, 2-3 parts of no injection in summer and 2-3 parts of injection in winter. The prepared disinfection aqueous solution of the medicine raw materials are mixed according to volume ratio to prepare the acupoint injection medicine. The acupoint injection medicine for treating rheumatic arthralgia is used for purifying blood, treating various rheumatic arthralgia and other rheumatism illness. The application method of the medicine is acupoint injection, and the acupoint injection medicine is capable of integral adjusting, and has the functions of qi-blood interacting, nourishing liver and kidney, reinforcing bone and nourishing marrow, invigorating spleen and disinhibiting dampness, promoting blood circulation to remove blood stasis, and diminishing flammation and relieving pain. The medicine is safe and effective, and can thoroughly treat various rheumatic arthralgia and difficult illness.
Owner:李梦珂

Traditional Chinese medicine for treating cephalic wind syndrome

The invention discloses a traditional Chinese medicine for treating cephalic wind syndrome. The traditional Chinese medicine for treating cephalic wind syndrome comprises Chinese herbs: Fructus Cassiae, Herba Lythri Salicariae, Cortex Capparis Dodinteri Radicis, Radix Mahoniae, Fructus seu Herba Pittospori Glabrati, Herba Hypecoi Leptocarpi, Cauli Tinosporae Crispae, Caulis Mahoniae, Herba Thalictri Squarrosi, Flos Micheliae Yunnanensis, Radix Aristolochiae Championii, Fructus Gleditsiae, Folium Clerodendri Trichotomi, Radix Aristolochiae Heterophyllae, Herba Lycopodii, Herba Paederiae, Cortex Daphnes Giraldii Radicis seu Caulis, Herba Clematidis Intricatae, and Herba Antenori. The traditional Chinese medicine made from all-natural Chinese herbs treats both symptoms and root causes, has clear therapeutic effects without toxic and side effects and ensures rarity of relapse.
Owner:张苗

Traditional Chinese medicine composite of daphne giraldii nitsche and liquorice, preparation method of composite, and application of composite in preparation of medicine treating rheumatoid arthritis

A traditional Chinese medicine composite of daphne giraldii nitsche and liquorice is characterized in that the weight ratio of bulk drugs, namely, the weight ratio of the daphne giraldii nitsche to the liquorice ranges from 3:1 to 3:2.5, the optimized weight ratio of the daphne giraldii nitsche to the liquorice ranges from 3:1 to 3:2 or 3:1.8, and the optimal ratio of the daphne giraldii nitsche to the liquorice is 3:1.5; the concentrated water of the bulk drugs is adopted to perform decoction so as to realize the further optimization, to be specific, daphne giraldii nitsche medical material coarse powder and liquorice decoction pieces are taken, and decoction are performed for two times through adding water respectively, water with the volume 10 times as large as the volume of the daphne giraldii nitsche medical material corase powder and the liquorice decoction pieces is added for decoction for 2 hours for the first time, and water with the volume 5 times as large as the volume of the daphne giraldii nitsche medical material corase powder and the liquorice decoction pieces is added for decoction for 1 hour for the second time, and the decoction water is mixed and concentrated, so as to obtain the final product. The traditional Chinese medicine composite of the daphne giraldii nitsche and the liquorice is helpful in preventing inflammation, alleviates an AA symptom, and improves the immunologic function and a tissue structure of an AA rat; the compatibility function of the daphne giraldii nitsche and liquorice is superior to the function of the single adopted daphne giraldii nitsche, and the pesticide effect of the compatibility group with the ratio being 3:2 of the daphne giraldii nitsche to the liquorice is superior to the compatibility group with the ratio being 3:1 of the daphne giraldii nitsche to the liquorice; the capability of the traditional Chinese medicine composite for reducing the content of serum inflammation cell factors as TNF-Alpha and IL-1 Beta is likely to be one of the mechanisms for treating rheumatoid arthritis.
Owner:NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE

Anti-tumor traditional Chinese medicine composition and preparation method and application thereof

The invention discloses an anti-tumor Chinese medicine composition and a preparation method and application thereof. The Chinese medicine composition is prepared with the following raw materials in parts by weight: 10-100 parts of ligusticum wallichii, 10-200 parts of corydalis amabilis, 1-50 part(s) of prepared monkshood, 10-300 parts of daphne giraldii nitsche, 10-100 parts of gentiana macrophylla and 1-50 part(s) of venenum bufonis. The traditional Chinese medicine composition provided by the invention is prepared according to the formula of the traditional Chinese medicine based on the theory of the traditional Chinese medicine and the theory of syndrome differentiation and treatment, and the results of in vivo and in vitro experiments show that the traditional Chinese medicine composition provided by the invention has the functions of inhibiting the growth of the tumor cell and promoting the apoptosis of the tumor cell, and has a strong function of killing a variety of tumor cells. Therefore, the scope of application of the traditional Chinese medicine composition is wide. Moreover, the extract of the traditional Chinese medicine composition provided by the invention can be prepared as various preparations, can be taken conveniently in clinical treatment and has no toxic and side effect in the long-term use.
Owner:雷允上药业集团有限公司

Cauliflower seed blender

The invention mainly relates to the technical field of planting, and discloses a cauliflower seed blender; the cauliflower seed blender is prepared from linden viburnum, hemsleya amabilis, trichosanthes cucumeroides, hypericum sampsonii hance, hypericum ascyron, daphne giraldii, deutzia scabra, weigela hortensis, alchornea davidii, lycoris squamigera, hypericum attenuatum, citric acid, chitosan, and sodium selenite. The cauliflower seed blender is rich in raw material, green and environment-friendly, safe and health, safe and harmless to human body, and easy to popularize; the economic income is added by 9.6%; multiple Chinese herbal medicinal ingredients are added, and the formula is scientific; through repeat extraction, the extraction rate is improved by 22.7%; the drug effect is supplemented each other, antibacterial and pesticidal, and can kill multiple pathogenic microorganisms; the cauliflower seed blender is rich in nutrition, and can improve embryo activity, speed up the seed germination, and improve the germination rate by 93%; the citric acid, chitosan, and sodium selenite are added to adjust the pH value for seed germination, provide rich nutrition components, inhibit propagation of pathogenic microorganism, enhance stress resistance of germ, speed up the seed germination, improve the transplanting survival rate to 98%, and save 10.3% of planting cost.
Owner:张殿兴

Preparation method and content measurement method of Daphne giraldii Nitsche total coumarin extract

The invention provides a preparation method and a content measurement method of a Daphne giraldii Nitsche total coumarin extract. The preparation method comprises: performing extraction by using a Daphne giraldii Nitsche herbal medicament as a raw material and water and / or C1 to C5 lower alcohol as a solvent to obtain an extract; mixing the extract with a solid diluent, adding an organic solvent for extraction, recovering the solvent to obtain a solid extract, or adding the extract into water for suspension, adding the organic solvent for extraction and receovering the solvent to obtain the solid extract; adding the solid extract into water for suspension, performing chromatography by macroporous adsorption resin in a way of eluting by using water or the aqueous solution of the C1 to C5 lower alcohol as a flowing phase to remove impurities first, then using the aqueous solution of the lower alcohol for elution and finally collecting eluent; reclaiming the solution from a previous halfpart of eluent to obtain a solid which is part of the Daphne giraldii Nitsche total coumarin extract; and reclaiming the solution from the later half part of eluent to obtain a solid, adding water into the solid for suspension, extracting the solid with an organic solvent and recovering an organic layer to obtain the solid which is the other part of the Daphne giraldii Nitsche total coumarin extract.
Owner:GUANGZHOU LIXIN PHARM CO LTD
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