8 results about "Nostoc commune" patented technology

This invention provides a method for extracting polysaccharides from *Nostoc commune* with immunomodulatory activity. Belonging to the field of functional food technology, the method includes the following steps: pulverizing dried *Nostoc commune* into powder; using the powder obtained in step S1 as raw material, extracting with 60% ethanol under ultrasonic assistance to obtain a crude polysaccharide extract; removing the ethanol from the crude polysaccharide extract in step S2 using a vacuum rotary evaporator, precipitating the polysaccharide from a 90% ethanol solution, and then dialyzing and freeze-drying it to obtain purified crude polysaccharide; eluting the purified crude polysaccharide using a Sephadex G-75 column, collecting the eluent in separate tubes and plotting elution curves, collecting the eluent from the tube with the peak on the elution curve and freeze-drying it to obtain a low molecular weight polysaccharide, which is NCEP.

The utility model discloses a nostoc sphaeroids kutz processing shaping tray, and relates to the technical field of nostoc sphaeroids kutz processing. The material tray comprises a material tray body, a plurality of screening holes are formed in the bottom face of the material tray body in a penetrating mode, a blocking mechanism is arranged at the bottom of the material tray body, supporting assemblies are arranged on the front side and the rear side of the material tray body, and shaking mechanisms used for controlling the material tray body to transversely move in a reciprocating mode are installed on the surfaces of the supporting assemblies. The blocking mechanism comprises a first motor, the first motor is fixedly installed on the bottom face of the tray body, the output end of the first motor is fixedly connected with a first rotating shaft, the surface of the first rotating shaft is fixedly connected with a connecting rod, and the end of the connecting rod is fixedly connected with a mounting frame. According to the nostoc sphaeroids kutz screening device, the effect of conveniently and rapidly screening nostoc sphaeroids kutz is achieved in the using process, nostoc sphaeroids kutz of the unified specification can be conveniently shaped, meshes can be automatically cleaned after screening is completed, time and labor are saved, and the situation that nostoc sphaeroids kutz is clamped to affect subsequent screening is avoided

This invention relates to the field of genetic engineering technology, specifically to a recombinant plasmid, its construction method, and its application in enhancing the synthesis of pseudocladocerin from Nostoc flagelliforme. The recombinant plasmid includes a vector plasmid, and sgRNA target sequences, HR homologous sequences, psbA promoter sequences, and other components linked to the vector plasmid. ScyR Regulatory genes. This recombinant plasmid can be used in black moss. MAAs Overexpression of key regulatory genes at gene cluster sites significantly enhanced the pseudocranophane synthesis capacity of Nostoc commune. The Nostoc mutant obtained by transforming this recombinant plasmid into Nostoc commune exhibited a pseudocranophane content 2.4 times higher than that of wild-type Nostoc commune after 15 days of culture.

The invention discloses a culture method for improving the solid content and group perfectness ratio of nostoc sphaeroids kutz, which comprises the following steps: providing an inoculation culture medium with a specific formula, and adjusting the pH value to be greater than or equal to 7.0; a nostoc sphaeroids kutz strain is inoculated into the culture medium, and aerobic culture is carried out for more than 30 days under the conditions that the temperature is 10-32 DEG C, the illumination intensity is 20-1200 and the light wavelength is 610-680 nm. The key point of the inoculation culture medium is that the inoculation culture medium contains dipotassium phosphate, magnesium sulfate, calcium chloride, citric acid, ferric ammonium citrate, disodium ethylene diamine tetraacetate, sodium carbonate, sodium nitrate and trace elements according to a specific ratio. By optimizing the synergistic effect of the components of the culture medium and the irradiation of the red light with the specific wavelength, the mechanical strength of the colloid sheath of the nostoc sphaeroids kutz population can be remarkably enhanced, the solid content and the population perfectness ratio of the nostoc sphaeroids kutz population are effectively improved, the growth rate is promoted, and the shelf life of fresh products is greatly prolonged.

The application provides a kind of automatic cultivation equipment and method of Nostoc commune, comprising: the upper part of base is provided with multiple groups of culture barrels along the first direction and is spaced side by side, each group of culture barrels is provided with a plurality of culture barrels along the second direction, the first drain groove and the second drain groove along the second direction are provided on the base, the bottom of culture barrel is provided with valve port, one side of base is provided with collection barrel communicated with the second drain groove;Mounting bracket includes fixed frame extending along the second direction;Liquid adding assembly and cleaning device are movably arranged on fixed frame along the second direction;Cleaning device includes brush and driving device for driving brush to rotate and move up and down;First horizontal moving device is arranged on fixed frame, for driving liquid adding assembly and cleaning device to move.This device can realize automatic production of culture Nostoc commune and automatic cleaning of culture barrel, which not only saves manpower, reduces cost, but also improves production efficiency, reduces waste of culture solution, and improves injection accuracy of culture solution.

The invention discloses a preparation method of a targeted biological bacterium and desert microalgae coupled sand stabilization composition. The preparation method comprises the following preparation steps: preparing a culture medium; the biomass of the fermentation liquor is larger than or equal to 2.0 * 10 < 9 > CFU.mL < 1 >, and the EPS yield of the fermentation liquor is larger than or equal to 10 g.L < 1 >. Mixing the targeted biological agent with the nostoc solution according to a volume ratio of 1: 1 to form a mixture; a synergist is added into the mixture; the invention relates to the technical field of desertification control and ecological restoration, bacteria and algae are coupled to form an initial biological membrane in a short time and then form a stable biological crust, so that wind erosion is effectively resisted; the microbial inoculum still keeps high activity under the conditions of pH 8.5 and high temperature, and is especially suitable for northwest desert environment; the organic soil conditioner in the composition can effectively reduce the soil salinity and alkalinity, form a granular structure and increase the soil organic matter content, industrial-grade raw materials and agricultural wastes are adopted, the culture medium cost is low, and the economic feasibility of large-scale application and popularization is achieved.

This invention relates to the field of food processing equipment technology and discloses a chain conveyor device for raw materials of Ge Xian rice porridge, comprising: a ring chain for conveying raw materials of Ge Xian rice porridge; the ring chain includes several discs; a sprocket for driving the ring chain to rotate; a first bevel gear connected to the discs; a second bevel gear rotatably mounted on the sprocket and meshing with the first bevel gear; and a drive structure for driving the second bevel gear to rotate. The drive structure of this invention causes the second bevel gear to drive the first bevel gear to rotate, thereby causing the discs passing through the sprocket to rotate. During the rotation of the discs through the sprocket, the discs simultaneously rotate, changing their state. When the discs enter the conveying pipe, different positions of the discs contact the inner side of the curved pipe, thus avoiding continuous wear on one side of the discs, helping to improve the service life of the discs, extend the disc replacement cycle, reduce downtime losses, improve production efficiency, and enhance economic benefits.

The application discloses a water stress protein WSP derived from Nostoc commune and application of the water stress protein WSP in skin protection, and belongs to the technical field of biotechnology and skin care. The amino acid sequence of the water stress protein WSP is shown as SEQ ID NO: 2, and the DNA sequence for coding the WSP protein is shown as SEQ ID NO: 1. The WSP protein of the application can significantly improve the survival rate of skin cells (such as HaCaT cells) under extreme dehydration conditions, protect the activity of key metabolic enzymes (such as lactate dehydrogenase), and effectively reduce the level of intracellular reactive oxygen species, and the comprehensive protection efficiency is superior to that of the existing moisturizing agent hyaluronic acid. The WSP protein provided by the application can be applied to skin care products such as face cream, essence and spray as an active ingredient, and is used for relieving dry skin, repairing skin barrier and antioxidation.