15 results about "Nostoc commune" patented technology

The invention discloses a moss-microorganism synergistic nitrogen fixation bionic regreening method and system for a drought-resistant mine rock slope, and belongs to the technical field of ecological environment restoration. The method comprises the following steps: (1) slope pretreatment: drilling anchoring holes; (2) layered construction: (1) a fixation layer: spraying mixed slurry prepared from amylase and plant fibers to form a net-shaped substrate with the thickness of 1-2cm; (2) a nutrient layer: spraying a mixture of a complex microbial inoculant and a slow release fertilizer, wherein the thickness is 4cm; the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens), and the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens). And (3) a water retention layer: sodium polyacrylate resin is spread, the particle size of the sodium polyacrylate resin is 1-0.5 mm, soil covering and compaction are performed until the thickness of the water retention layer is 1-1.5 cm, and the use amount of the sodium polyacrylate resin is 2-1.5 wt% of the weight of the soil. According to the method, the nitrogen fixation efficiency can be improved by 40-60%, and the soil moisture content is kept gt under the drought condition; the survival rate of the seedlings is increased to 85% or above, the anti-scouring capacity of the side slope is improved by 50%, and the vegetation coverage rate of 70% or above is achieved within 6 months.

This invention provides a method for extracting polysaccharides from *Nostoc commune* with immunomodulatory activity. Belonging to the field of functional food technology, the method includes the following steps: pulverizing dried *Nostoc commune* into powder; using the powder obtained in step S1 as raw material, extracting with 60% ethanol under ultrasonic assistance to obtain a crude polysaccharide extract; removing the ethanol from the crude polysaccharide extract in step S2 using a vacuum rotary evaporator, precipitating the polysaccharide from a 90% ethanol solution, and then dialyzing and freeze-drying it to obtain purified crude polysaccharide; eluting the purified crude polysaccharide using a Sephadex G-75 column, collecting the eluent in separate tubes and plotting elution curves, collecting the eluent from the tube with the peak on the elution curve and freeze-drying it to obtain a low molecular weight polysaccharide, which is NCEP.

This invention proposes a Bifida ferment lysate, its preparation method, and its application, belonging to the field of cosmetic technology. The first fermentation product is obtained by enzymatic fermentation of Ganoderma lucidum, Poria cocos, Nostoc commune, oats, lactoferrin, and ginseng. Konjac gum hydrolysate and collagen and glycosaminoglycans extracted from tilapia skin are added, and fermentation continues to obtain a second fermentation product. Modified lysozyme is added for enzymatic hydrolysis, followed by centrifugation. The supernatant is collected, and copper and calcium salts are added and stirred to obtain a Cu-Ca chelated fermentation lysate. The product is then encapsulated to obtain the Bifida ferment lysate, which has excellent whitening, moisturizing, antibacterial, soothing, skin-repairing, and pH-regulating effects on the skin. It is safe, non-toxic, non-irritating to the skin, highly absorbable by the skin, and has a long shelf life, showing broad application prospects.

The invention particularly relates to the technical field of biology, and provides a nostoc sphaeroids kutz peptide composition with anti-inflammatory activity, an anti-inflammatory active peptide and a preparation method and application of the nostoc sphaeroids kutz peptide composition and the anti-inflammatory active peptide, the nostoc sphaeroids kutz peptide composition contains the anti-inflammatory active peptide, and the molecular mass of the anti-inflammatory active peptide is smaller than 500 Da; the amino acid sequence of the anti-inflammatory active peptide is one or more of PAG, LVG, LGV and PVL; the preparation method comprises the following steps: crushing nostoc sphaeroids kutz and breaking walls, adding a phosphate buffer solution to homogenate, taking supernate, adding an ethanol solution, separating to obtain polypeptide supernate, and carrying out rotary evaporation and freeze drying to obtain polypeptide crude extract powder; and dissolving the polypeptide crude extract powder in a phosphate buffer solution, carrying out in-vitro gastrointestinal protease hydrolysis and Caco-2 cell transmembrane transport test, and freeze-drying to obtain the anti-inflammatory active peptide after transmembrane transport. The nostoc sphaeroids kutz peptide composition containing the anti-inflammatory active peptide has good anti-inflammatory activity, can inhibit secretion of inflammatory factors such as NO and TNF-alpha in macrophages, and can be used for preparing anti-inflammatory drugs, functional food or feed additives.

The utility model discloses a nostoc sphaeroids kutz processing shaping tray, and relates to the technical field of nostoc sphaeroids kutz processing. The material tray comprises a material tray body, a plurality of screening holes are formed in the bottom face of the material tray body in a penetrating mode, a blocking mechanism is arranged at the bottom of the material tray body, supporting assemblies are arranged on the front side and the rear side of the material tray body, and shaking mechanisms used for controlling the material tray body to transversely move in a reciprocating mode are installed on the surfaces of the supporting assemblies. The blocking mechanism comprises a first motor, the first motor is fixedly installed on the bottom face of the tray body, the output end of the first motor is fixedly connected with a first rotating shaft, the surface of the first rotating shaft is fixedly connected with a connecting rod, and the end of the connecting rod is fixedly connected with a mounting frame. According to the nostoc sphaeroids kutz screening device, the effect of conveniently and rapidly screening nostoc sphaeroids kutz is achieved in the using process, nostoc sphaeroids kutz of the unified specification can be conveniently shaped, meshes can be automatically cleaned after screening is completed, time and labor are saved, and the situation that nostoc sphaeroids kutz is clamped to affect subsequent screening is avoided

This invention relates to the field of genetic engineering technology, specifically to a recombinant plasmid, its construction method, and its application in enhancing the synthesis of pseudocladocerin from Nostoc flagelliforme. The recombinant plasmid includes a vector plasmid, and sgRNA target sequences, HR homologous sequences, psbA promoter sequences, and other components linked to the vector plasmid. ScyR Regulatory genes. This recombinant plasmid can be used in black moss. MAAs Overexpression of key regulatory genes at gene cluster sites significantly enhanced the pseudocranophane synthesis capacity of Nostoc commune. The Nostoc mutant obtained by transforming this recombinant plasmid into Nostoc commune exhibited a pseudocranophane content 2.4 times higher than that of wild-type Nostoc commune after 15 days of culture.

The invention discloses a culture method for improving the solid content and group perfectness ratio of nostoc sphaeroids kutz, which comprises the following steps: providing an inoculation culture medium with a specific formula, and adjusting the pH value to be greater than or equal to 7.0; a nostoc sphaeroids kutz strain is inoculated into the culture medium, and aerobic culture is carried out for more than 30 days under the conditions that the temperature is 10-32 DEG C, the illumination intensity is 20-1200 and the light wavelength is 610-680 nm. The key point of the inoculation culture medium is that the inoculation culture medium contains dipotassium phosphate, magnesium sulfate, calcium chloride, citric acid, ferric ammonium citrate, disodium ethylene diamine tetraacetate, sodium carbonate, sodium nitrate and trace elements according to a specific ratio. By optimizing the synergistic effect of the components of the culture medium and the irradiation of the red light with the specific wavelength, the mechanical strength of the colloid sheath of the nostoc sphaeroids kutz population can be remarkably enhanced, the solid content and the population perfectness ratio of the nostoc sphaeroids kutz population are effectively improved, the growth rate is promoted, and the shelf life of fresh products is greatly prolonged.

The application provides a kind of automatic cultivation equipment and method of Nostoc commune, comprising: the upper part of base is provided with multiple groups of culture barrels along the first direction and is spaced side by side, each group of culture barrels is provided with a plurality of culture barrels along the second direction, the first drain groove and the second drain groove along the second direction are provided on the base, the bottom of culture barrel is provided with valve port, one side of base is provided with collection barrel communicated with the second drain groove;Mounting bracket includes fixed frame extending along the second direction;Liquid adding assembly and cleaning device are movably arranged on fixed frame along the second direction;Cleaning device includes brush and driving device for driving brush to rotate and move up and down;First horizontal moving device is arranged on fixed frame, for driving liquid adding assembly and cleaning device to move.This device can realize automatic production of culture Nostoc commune and automatic cleaning of culture barrel, which not only saves manpower, reduces cost, but also improves production efficiency, reduces waste of culture solution, and improves injection accuracy of culture solution.

This invention provides a feeding device for processing Nostoc commune (Ge Xian Mi) food products, relating to the field of food processing technology. The feeding device includes a feeding box, with a shell fixedly installed at the center of the front end of the feeding box. First transmission rollers are rotatably mounted on both sides inside the feeding box, and a transverse transmission belt is sleeved between the two first transmission rollers. A support base is fixedly installed inside the shell and within the center of the transverse transmission belt. Second transmission rollers are rotatably mounted at both ends of the support base, and a longitudinal conveyor belt is sleeved between the two second transmission rollers. First protective shells are fixedly installed at both ends of the feeding box and on one side of the shell. Two protective shells are provided at the rear end, located on opposite sides of the rear end of the feeding box. This feeding device can screen Nostoc commune of different sizes during the feeding process and also has a drying function, improving the functionality of the feeding device.

The invention relates to the technical field of food processing equipment, and discloses a nostoc sphaeroids kutz porridge raw material pipe chain type conveying device which comprises an annular chain used for conveying nostoc sphaeroids kutz porridge raw materials; the annular chain comprises a plurality of discs; the chain wheel is used for driving the annular chain to rotate; the first bevel gear is connected with the disc; the second bevel gear is rotationally mounted on the chain wheel and meshed with the first bevel gear; the driving structure is used for driving the second bevel gear to rotate; the driving structure enables the second bevel gear to drive the first bevel gear to rotate, then the disc passing through the chain wheel rotates, the state of the disc is changed when the disc passes through the chain wheel to rotate, different positions of the disc make contact with the inner side of the bent pipeline when the disc enters the conveying pipe, and therefore continuous abrasion of the single side of the disc is avoided, and the service life of the disc is prolonged. And the service life of the disc is prolonged, the replacement period of the disc is prolonged, the shutdown loss is reduced, the production efficiency is improved, and the economic benefit is improved.

The invention discloses a preparation method of a targeted biological bacterium and desert microalgae coupled sand stabilization composition. The preparation method comprises the following preparation steps: preparing a culture medium; the biomass of the fermentation liquor is larger than or equal to 2.0 * 10 < 9 > CFU.mL < 1 >, and the EPS yield of the fermentation liquor is larger than or equal to 10 g.L < 1 >. Mixing the targeted biological agent with the nostoc solution according to a volume ratio of 1: 1 to form a mixture; a synergist is added into the mixture; the invention relates to the technical field of desertification control and ecological restoration, bacteria and algae are coupled to form an initial biological membrane in a short time and then form a stable biological crust, so that wind erosion is effectively resisted; the microbial inoculum still keeps high activity under the conditions of pH 8.5 and high temperature, and is especially suitable for northwest desert environment; the organic soil conditioner in the composition can effectively reduce the soil salinity and alkalinity, form a granular structure and increase the soil organic matter content, industrial-grade raw materials and agricultural wastes are adopted, the culture medium cost is low, and the economic feasibility of large-scale application and popularization is achieved.

This application discloses the application of Nostoc commune extract in cigarettes, particularly the application of extracts prepared from Nostoc commune via the Maillard reaction. The Nostoc commune extract obtained through the Maillard reaction overcomes the unpleasant odors of extracts obtained by other processes and extracts the grassy aroma of Nostoc commune, making it suitable as a flavoring agent in cigarettes and enriching the smoking experience.

The invention discloses composite fungal gel for preventing and treating monochamus alternatus hope as well as a preparation method and application of the composite fungal gel. The preparation method of the composite fungal gel comprises the following steps: forming a crosslinkable oligomer from polyvinyl alcohol (PVA) and sodium alginate (SA); microcoleus and nostoc are added, polycoleus secretes various cyanobacteria polysaccharides, and nostoc is filamentous and forms a biological protection layer; adding a metarhizium anisopliae and beauveria bassiana spore suspension to infect monochamus alternatus; longhorn beetle pheromone is added to attract monochamus alternatus to gather; the curing agent is added to trigger curing, the humectant is added to ensure the activity and the continuous state of the gel, the long-acting killing effect is kept, the gel can be sprayed in a large range through an unmanned aerial vehicle, the operation range is greatly expanded, and the labor cost expenditure is reduced.

This invention relates to the field of food processing equipment technology and discloses a chain conveyor device for raw materials of Ge Xian rice porridge, comprising: a ring chain for conveying raw materials of Ge Xian rice porridge; the ring chain includes several discs; a sprocket for driving the ring chain to rotate; a first bevel gear connected to the discs; a second bevel gear rotatably mounted on the sprocket and meshing with the first bevel gear; and a drive structure for driving the second bevel gear to rotate. The drive structure of this invention causes the second bevel gear to drive the first bevel gear to rotate, thereby causing the discs passing through the sprocket to rotate. During the rotation of the discs through the sprocket, the discs simultaneously rotate, changing their state. When the discs enter the conveying pipe, different positions of the discs contact the inner side of the curved pipe, thus avoiding continuous wear on one side of the discs, helping to improve the service life of the discs, extend the disc replacement cycle, reduce downtime losses, improve production efficiency, and enhance economic benefits.

The application discloses a water stress protein WSP derived from Nostoc commune and application of the water stress protein WSP in skin protection, and belongs to the technical field of biotechnology and skin care. The amino acid sequence of the water stress protein WSP is shown as SEQ ID NO: 2, and the DNA sequence for coding the WSP protein is shown as SEQ ID NO: 1. The WSP protein of the application can significantly improve the survival rate of skin cells (such as HaCaT cells) under extreme dehydration conditions, protect the activity of key metabolic enzymes (such as lactate dehydrogenase), and effectively reduce the level of intracellular reactive oxygen species, and the comprehensive protection efficiency is superior to that of the existing moisturizing agent hyaluronic acid. The WSP protein provided by the application can be applied to skin care products such as face cream, essence and spray as an active ingredient, and is used for relieving dry skin, repairing skin barrier and antioxidation.