Nostoc commune antitumor polysaccharide as well as preparation method and application thereof

An anti-tumor and ground fungus technology, which is applied in the fields of anti-tumor drugs, applications, food preparation, etc., can solve the problems that the anti-tumor activity of ground fungus polysaccharides has not been seen, and achieve the inhibition of cancer cell proliferation, great development prospects and application value, The effect of good anticancer activity

Inactive Publication Date: 2014-12-10
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the polysaccharides of fungus fungus have antioxidant and antibacterial activities, as well as anti-fatigue, anti-virus, hypoglycemic, hypolipidemic, and anti-inflammatory functions, but no research on the anti-tumor activity of fungus polysaccharides has been seen

Method used

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  • Nostoc commune antitumor polysaccharide as well as preparation method and application thereof
  • Nostoc commune antitumor polysaccharide as well as preparation method and application thereof
  • Nostoc commune antitumor polysaccharide as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The preparation method of embodiment 1 fungus anti-tumor polysaccharide (NVPS)

[0022] (1) Dry the natural fungus at 60° C. for 2 hours, and crush it.

[0023] (2) Take 100 g of ground fungus powder, add 5 L of distilled water, heat and reflux for 3 hours, let stand, filter with double-layer gauze, take the supernatant, repeat the extraction once as above, combine the two filtrates, evaporate and concentrate to 1000 ml under reduced pressure;

[0024] (3) add 100ml potassium ferrocyanide (K 4 [Fe(CN) 6 ]·3H 2 O) (10.6%), shake well, then add 100ml zinc acetate (C 4 h 8 o 4 Zn) (21.9%), shake evenly to precipitate protein, place at room temperature for 30 minutes, centrifuge at 5000rpm for 20 minutes, take supernatant, evaporate and concentrate to 100ml under reduced pressure;

[0025] (4) The concentrated solution obtained in step (3) is dialyzed in distilled water with a dialysis bag with a cutoff of 3000, and the water is changed once every 12 hours until the c...

Embodiment 2

[0029] Example 2: Select MCF-7 cells in the logarithmic growth phase, make the cells into a cell suspension, count the cells, 4×10 cells per well 4 Inoculated into a 96-well plate at 37°C, 5% CO 2 Culture in the incubator for 24 hours and after the cells adhere to the wall, discard the old medium, add medium with different polysaccharide final concentrations (0, 25, 50, 100, 200, 300ug / ml), set 5 parallel wells, and culture The plate was further incubated for 24 hours, and 20 μL of 5.0 mg / mL MTT was added to each well, and incubated for another 4 hours. Remove the culture medium in the plate, add 150 μL of DMSO to each well to dissolve the formazan crystals produced by the living cells, shake on a shaker for 10 min, and measure the absorbance of each well at a wavelength of 570 nm with a microplate reader. A concentration was considered toxic to cells if the decrease in absorbance of wells containing polysaccharide extract was greater than 10% compared to control wells (ie, i...

Embodiment 3

[0032] Select MCF-7 cells in the logarithmic growth phase, adjust the cell concentration to 8×10 per well 3 cells / 100 μl of culture medium and added to a 96-well culture plate. 37°C, 5% CO 2 Incubate overnight in an incubator. After the cells adhered to the wall, the culture plate was taken out, and the culture medium containing different concentrations (0, 25, 50, 100, 200, 300ug / ml) of Auricularia polysaccharide was replaced, and 5 parallel wells were set. The culture plate was put back into the incubator to continue culturing for 24 hours and 36 hours, and 20 μL of 5.0 mg / mL MTT was added to each well, and the incubation was continued for 4 hours. Aspirate the culture medium in the plate, add 150 μl of DMSO to each well to dissolve the formazan crystals produced by the living cells, shake on a shaker for 10 min, and measure the absorbance of each well with a microplate reader at a wavelength of 570 nm.

[0033] The fungus polysaccharide (NVPS) obtained in the present inv...

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Abstract

The invention provides a nostoc commune antitumor polysaccharide and a preparation method thereof as well as application of the polysaccharide in preparing an antitumor drug and healthcare food. The preparation method for the nostoc commune antitumor polysaccharide comprises the following steps: drying and crushing pure natural nostoc commune, weighing proper amount of the dried and crushed nostoc commune, adding distilled water to reflux and extract the weighed nostoc commune, and combining extracting liquor; carrying out rotary evaporation and concentration on the extracting liquor to an appropriate amount, adding 10.6% potassium ferrocyanide liquor and 21.9% zinc acetate liquor in a volume ratio being 1:1 to precipitate proteins, absorbing liquid supernatant after centrifuging the mixture; carrying out rotary evaporation and concentration on the mixture under reduced pressure to a certain volume, adding absolute ethyl alcohol to separate out white flocculus after dialyzing concentrated liquor by virtue of distilled water, and freezing and drying in vacuum after centrifuging to obtain the nostoc commune polysaccharide. According to an in-vitro antitumor experiment, the polysaccharide can be used for obviously inhibiting multiplication of human breast cancer cells, is free of cell toxicity, has good antitumor activity, and provides possibility for developing and researching novel anticancer healthcare products or drugs.

Description

technical field [0001] The invention relates to the preparation and application of blue-green algae plant polysaccharides, in particular to a fungus anti-tumor polysaccharide and its preparation method, as well as its application in the preparation of anti-tumor drugs and natural health food. Background technique [0002] Ground fungus, scientific name Nostoc commune Vauch., commonly known as ground vegetable, alias lichen, ground vegetable mushroom, ground soft child, ground vegetable peel, ground ear, is a plant of the genus Nostoc of the algae Cyanobacteria Nostocaceae (nostocaceae) Sheet-shaped algae of nostoc commune vauch. It generally grows on the surface of moist sandy soil, often mixed between the stems and leaves at the base of weeds, the base of large trees and moss flora. Ground fungus is distributed almost all over the country, and wild resources are widely distributed in the Northeast, especially in the Changbai Mountains, and it also has a high yield in Shanx...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A23L1/29A23L1/30A61K8/73A23L33/10
Inventor 李卓玉郭敏郭松佳单树花李宗伟卢德赵李汉卿
Owner SHANXI UNIV
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