Pollution controlling method in cultivating nostoc and nostoc commune

A control method, the technology of fungus, applied in the direction of botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems affecting the quality of finished products, the reduction of output, and the specific method for the pollution control of fungus has not been proposed, so as to achieve high safety , Simple equipment, guaranteed quality effect

Inactive Publication Date: 2004-11-10
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

[0003] Gexianmi and ground fungus will be threatened by various polluting organisms during the cultivation process, mainly including miscellaneous algae, zooplankton, and mosquito larvae. Pollution organisms will reduce the yield, and mosquito larvae feed on Nostoc, which is very difficult to harvest. Difficult to remove, affecting the quality of finished products; in severe cases, making cultivation impossible
Chinese patent document: A method for cultivating Gexianmi, classification number: C12N 1 / 12, application date: 1999.10.22, publication date: 2001.05.09, application number: 99120005.5, applicant: Institute of Hydrobiology, Chinese Academy of Sciences, The patent only mentions that the culture medium needs to be completely replaced and the reactor should be sterilized when the microbial contamination is serious in the cultivation of Gexianmi, and no specific operation method is proposed.
Chinese patent document: A method for cultivating ground fungus, classification number: C12N 1 / 12, application number: 03119091.X, applicant: Institute of Hydrobiology, Chinese Academy of Sciences, this patent only involves the cultivation of ground fungus when the pollution is serious. Completely replace the culture medium, sterilize the reactor or the culture pool, and no specific method for the control of the contamination of the fungus
No method for prevention and control of pollution in the culture of Gexianmi and ground fungus has been disclosed or used after searching

Method used

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Embodiment Construction

[0008] Artificially cultured Gexianmi and ground fungus are mainly spherical, and less dumbbell-shaped or oval; it takes 2-3 months to grow from algae filaments or algae segments to a finished product with a diameter of 5-7 mm; During the period when the ground fungus is cultivated into a finished product of 5-7 mm by algae silk or algae growth section, the culture solution is changed every 8-20 days. The steps of the prevention and control method of pollution in the cultivation of Gexianmi and ground fungus are as follows:

[0009] 1. Pure culture is used in the seed production stage: Erlenmeyer flasks, glass homogenizers, coating sticks, pipettes, culture solutions, water, and culture containers used in the preparation of algae filaments or algae breeding stages must be sterilized in high-pressure steam For 15 minutes, the preparation of algae filaments is carried out under sterile conditions, and sterile gas is introduced when ventilation is required; the algae filaments or...

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Abstract

The invention discloses a control method of pollution of nostoc and agaric culture mediums, firstly adopting pure cultivation in the strain production; secondly, before inoculating, washing culture tank and container; thirdly, washing or screening algae; fourthly, filtering the culture solution by gauze; fifthly, killing or eliminating planktons. It has simple and easy operation, high efficiency, convenient operation, low cost, able to effectively control the quantities of living pollutants in the culture solution within 1% during cultivating, and suitable to control production pollution in factories.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, and more specifically relates to a pollution control method in the cultivation of Gexianmi and ground fungus. Background technique [0002] Nostoc is a kind of algae group with wide distribution, complex ecological environment and various morphological structures in the cyanophyta Cyanophyta Cyanophyceae Cyanophyceae segmental algae order Hormogonales Nostocaceae. , leaf-like, filamentous, bubble-like and other shapes, hollow or solid, floating or growing. The protophyte consists of many fine filaments with a diameter of a few microns assembled into a group visible to the naked eye, and the shape of the group varies with the "species", such as Nostoc flageliforme Kütz., Nostoc commune, Ge Xianmi (Nostoc sphaeroides) are in the shape of hair, flat ears or small balls (beads) in the fresh state, and shrink to varying degrees after drying, all of which have rich nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00C12N1/12
CPCY02A40/80
Inventor 邓中洋胡征宇
Owner INST OF AQUATIC LIFE ACAD SINICA
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