Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof

An effective component and the technology of ancestors, which are applied in the preparation of anti-rheumatoid arthritis drugs by the effective components of Zushima. The effective components of Zushima and its preparation field can solve the problem of large side effects, dependence, Treat unoptimistic problems and achieve the effect of easy operation, low cost and safe medication

Inactive Publication Date: 2009-07-08
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although more and more researchers are devoted to this field, the treatment of RA is still not optimistic
[0003] At present, the drugs for treating RA can be roughly divided into traditional Chinese medicine and chemical drugs. Clinical application proves that chemical drugs are effective in treating RA, but long-term use has large side effects and is dependent. Generally, it only plays a short-term control role in clinical practice.

Method used

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  • Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof
  • Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof
  • Effective component group of stem-bark or root-bark of Daphne giraldii as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1 The preparation method of Zushima effective component group

[0084] 10 kg of crushed Zushima medicinal materials were soaked in 15L of 10% ethanol for 2 hours, put into a percolation bucket, and 100L of percolation solution was collected and concentrated. The concentrated solution was successively extracted with petroleum ether for three times, and the amount of petroleum ether was 1L each time. The extraction part of petroleum ether was discarded, and the extracted aqueous layer was extracted three times with ethyl acetate. The ester extract was recovered under reduced pressure with ethyl acetate, and the concentrate was further concentrated and spray-dried to obtain b extract. The aqueous layer liquid extracted with ethyl acetate is adsorbed on D101 type resin, and the impurities are first washed with 10% ethanol, and then eluted with 60% ethanol. Extract. The content of total coumarin was 78% and the content of total saponin was 65% by spectrophotomet...

Embodiment 2

[0085] Embodiment 2 The preparation method of Zushima effective component group

[0086] 10 kilograms of pulverized Zushima medicinal materials were loaded into a reflux device, and extracted with 8L, 8L, and 6L of 20% ethanol, respectively, for 1.5 hours (three times in total), combined the extracts, concentrated under reduced pressure to evaporate the ethanol, and extracted with petroleum ether respectively Three times, the amount of petroleum ether was 1 L each time, and the petroleum ether extract was discarded. The mother liquor extracted with petroleum ether was extracted three times with ethyl acetate, each time the amount of ethyl acetate was 1 L, the ethyl acetate was recovered under reduced pressure, concentrated, and dried in vacuo to obtain b extract. The aqueous layer liquid extracted with ethyl acetate was adsorbed on AB-8 resin, firstly washed with 10% ethanol to remove impurities, and then with 60% ethanol. Get c extract. The total coumarin content was 85% an...

Embodiment 3

[0087] Embodiment 3 The preparation method of Zushima effective component group

[0088]10 kg of crushed Zushima medicinal materials were soaked in 10 L of 40% ethanol for 12 hours, filtered, and the residue was soaked in 8 L of 40% ethanol for 12 hours, combined with two ethanol dipping solutions, ethanol was recovered under reduced pressure, and the concentrate was added with petroleum ether Extract three times, each time using 1L of petroleum ether, discard the petroleum ether extract, and extract the aqueous layer after petroleum ether extraction with ethyl acetate three times, each time using 1L of ethyl acetate, combine the ethyl acetate extracts, reduce The ethyl acetate was recovered by pressure, concentrated, and spray-dried to obtain b extract (total coumarin). The aqueous layer liquid extracted with ethyl acetate is adsorbed on XAD-4 resin, firstly washed with 20% ethanol to remove impurities, then eluted with 60% ethanol, concentrated the eluate, spray-dried to obt...

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Abstract

The invention relates to an effective component group of daphne giraldii nitsche for resisting rheumatoid arthritis with active ingredients of total coumarine and total saponin. The effective component group is prepared by the following methods according to a certain weight ratio: the daphne giraldii nitsche medicinal materials are ground into coarse powder which is first extracted by alcohol, and then the alcohol is recovered from the alcohol extraction liquid, water is added for scattering the alcohol extraction liquid, two organic solvents are used in sequence for extraction, recovery and concentration to first obtain a extractive matter (diterpenoid ingredient) and then obtain b extractive matter (total coumarine ingredient); a extractive matter(diterpenoid ingredient)is abandoned, macropore adsorption resin column is applied to liquid medicine obtained from the extraction of the b extractive matter, the liquid medicine is eluted by ethanol, the ethanol is recovered and concentrated to obtain c extractive matter (total saponin ingredient); finally, effective component group of daphne giraldii nitsche for resisting rheumatoid arthritis is obtained by mixing the total coumarine ingredient and the total saponin ingredient according to a certain weight ratio. The effective component group of daphne giraldii nitsche features simple preparation process, easy operation and low cost, in addition, no high temperature equipment or high voltage equipment is needed, the poisonous diterpenoid ingredient is removed, thus reducing toxicity and improving safety. The result of pharmacological tests indicates that the effective component group of the invention has significant effect on curing the rheumatoid arthritis and is superior to single ingredient total coumarin.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to an active component group of Zushima and a preparation method thereof, and the application of the active component group of Zushima in preparing an anti-rheumatoid arthritis drug. Background technique [0002] Rheumatoid arthritis (RA) is one of the most common bone and joint diseases with the highest disability rate. It is a systemic autoimmune disease characterized by chronic inflammation of the joint synovium. Its lesions mainly occur in the synovium, involving articular cartilage, ligaments, tendons and systemic tissues, causing joint tumors, followed by cartilage destruction and joint space changes. Narrow, advanced joint deformity, functional mobility impairment instant monument. The disease has a global distribution, the age of onset is mostly between 25-55 years old, and female patients are about 2-3 times that of male patients. %, is on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/83A61P29/00A61P19/02
Inventor 狄留庆吴皓许惠琴单进军静芸芸杜秋张新庄毕肖林赵晓莉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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