Determination method for viability of daphne giraldii seeds

A measurement method and viability technology, which is applied in the field of determination of the viability of Huang Ruixiang seeds, can solve problems such as excessive and unreasonable rooting and digging methods, difficulty in meeting production needs, and shortage of ancestral Asahara plants, and achieve easy operation and principle Reliable, reproducible results

Inactive Publication Date: 2014-04-09
甘肃凯源生物技术开发中心有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant Huang Ruixiang is currently a wild resource. It is the original plant of the traditional Chinese medicinal material Zushima. The used parts are the root bark and stem bark. Excessive and unreasonable root digging methods have seriously damaged plant resources, resulting in an extreme shortage of Zushima original plants; And because the seeds of Daphne chrysantha are hard and dormant, the germination rate is very low, which is difficult to meet the production needs; and seed viability is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The assay method for the viability of Daphne chrysanthemum seeds comprises the steps:

[0043] a) Cutting step: taking 500g of Daphne chrysanthemum seeds by cutting;

[0044] b) Clarity treatment step: removing the pericarp of the daphne japonica seeds by extrusion method, and cleaning them to obtain clean seeds;

[0045] c) Disinfection step: soak the clean seeds with 3% potassium permanganate for 5 minutes for disinfection;

[0046] d) Pre-soaking treatment step: place the sterilized clean seeds in a beaker filled with distilled water, and soak at a constant temperature of 25° C. for 12 hours under natural light;

[0047] e) The step of breaking hard seeds: Randomly extract the soaked seeds obtained in step d), polish the hard seed coat with sandpaper, and carry out several repeated experiments;

[0048] f) Seed coat treatment steps: without injuring the seed embryo, cut the seed coat longitudinally between the ventral and back of the seed with a scalpel, then gentl...

Embodiment 2

[0053] The assay method for the viability of Daphne chrysanthemum seeds comprises the steps:

[0054]a) Cutting step: taking 500g of Daphne chrysanthemum seeds by cutting;

[0055] b) Clarity treatment step: removing the pericarp of the Daphne japonica seeds by stirring method, and cleaning them to obtain clean seeds;

[0056] c) Disinfection step: the clean seeds are sterilized by soaking them in 1% potassium permanganate for 5 minutes;

[0057] d) Pre-soaking treatment step: place the sterilized clean seeds in a beaker filled with distilled water, and soak at a constant temperature of 25°C for 6 hours under natural light;

[0058] e) The step of breaking hard seeds: Randomly extract the soaked seeds obtained in step d), polish the hard seed coat with sandpaper, and carry out several repeated experiments;

[0059] f) Seed coat treatment step: without injuring the seed embryo, puncture the seed coat with a dissecting needle, and place the broken seed in a test tube;

[0060...

Embodiment 3

[0064] The assay method for the viability of Daphne chrysanthemum seeds comprises the steps:

[0065] a) Cutting step: taking 500g of Daphne chrysanthemum seeds by cutting;

[0066] b) Clarity treatment step: removing the pericarp of the daphne japonica seeds by extrusion method, and cleaning them to obtain clean seeds;

[0067] c) Disinfection step: soak the clean seeds with 5% potassium permanganate for 5 minutes for disinfection;

[0068] d) Pre-soaking treatment step: place the sterilized clean seeds in a beaker filled with distilled water, and soak at a constant temperature of 25° C. for 18 hours under natural light;

[0069] e) The step of breaking hard seeds: Randomly extract the soaked seeds obtained in step d), polish the hard seed coat with sandpaper, and carry out several repeated experiments;

[0070] f) Seed coat treatment steps: without injuring the seed embryo, cut the seed coat longitudinally between the ventral and back of the seed with a scalpel, then gentl...

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PUM

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Abstract

The invention belongs to a determination method for the viability of daphne giraldii seeds and belongs to the field of quality identification and cultivation of seeds of traditional Chinese medicinal materials. The method comprises the following steps: cutting seeds, carrying out purity treatment, disinfecting seed, pre-soaking the seeds, crushing hard seeds, and treating seed coats; dyeing; and determining the viability. According to the method provided by the invention, the viability of the daphne giraldii seeds can be rapidly and effectively determined and damages and death to the daphne giraldii seeds, caused by other determination methods, are reduced; the working dangers in an operation process are reduced; the method is simple and feasible, reasonable in principle, high in working efficiency and good in repeatability. The death to of the daphne giraldii seeds is 94.5%.

Description

technical field [0001] The invention belongs to the field of quality identification and cultivation of Chinese medicinal material seeds, in particular to a method for measuring the viability of Daphne chrysanthemum seeds. technical background [0002] Daphne giraldii Nitsche (Daphne giraldii Nitsche) belongs to Daphne giraldii Nitsche plant, also known as knotted flower and knotted incense. Distributed in my country's Shaanxi, Gansu, Qinghai, Henan and other provinces, Gansu Province is mainly distributed in Qilian Mountains and other hillside shrubs, forest margins, and valleys at an altitude of 2000-2600m. The root bark and stem bark of Huang Ruixiang are common traditional Chinese medicines for treating rheumatism and pain relief, commonly known as Zushima, which have the functions of lowering blood fat, anti-hemolytic activity, anti-tumor activity and strong analgesia, and can also be used to treat arthritis and analgesia , anti-inflammatory, surgical anesthesia, etc. ...

Claims

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Application Information

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IPC IPC(8): A01C1/02
Inventor 王勤礼闫芳毛著鸿吕彪高松许耀照
Owner 甘肃凯源生物技术开发中心有限责任公司
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