The invention discloses a fertilizer additive prepared from a cell extracting solution and a preparation method of the fertilizer additive. The fertilizer additive is prepared from, by weight, acrylic acid, starch, N,N'-methylene bisacrylamide, chitin, ethephon, humic acid powder, moss, seaweed, corn flour, malt, gelatin, brown sugar, yeast powder, biogas slurry, dried potato powder, dried potato powder raw materials and a proper amount of water. Cells of moss and cells of the seaweed are cultured, organic materials can be decomposed through reproduction of the cells to generate organic matter decomposer, meanwhile, the organic matter decomposer can be subjected to culture expansion by itself, a certain amount of yeast is added, one part of cells which cannot be decomposed by the seaweed cells can be decomposed through the yeast, nutrients are provided, and finally the fermentation materials and the seaweed cells are crushed and extracted. On the one hand, organic acid ingredients beneficial to growth of crops generated after decomposition are utilized, and meanwhile intracellular products released after rupture of the cells of the moss and the seaweed cells also achieve a critical function on growth of the crops.