On-site rapid culture expansion method of aerobic denitrifying bacteria

A technology of aerobic denitrifying bacteria and nitrifying bacteria, applied in the direction of bacteria, etc., can solve the problems of long storage time on site, affect the quality of bacteria, increase project costs, etc., achieve small investment, avoid negative effects, save transportation costs and The effect of time cost

Pending Publication Date: 2019-11-22
沐联环境科技(重庆)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the microbial agent must maintain the effective number of viable bacteria and the activity of the bacteria when it is applied, but the project situation is changing at any time and the dosage of the microbial agent ...

Method used

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  • On-site rapid culture expansion method of aerobic denitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 Shown, a kind of on-the-spot rapid expansion culture method of aerobic denitrifying bacteria, culture condition: temperature 20 ℃; pH=7; Dissolved oxygen 4mg / L;

[0032] Including the following steps:

[0033] (1) The laboratory inoculates and expands the aerobic denitrifying bacteria liquid, as the aerobic denitrifying bacteria seed liquid for on-site expansion;

[0034] (2) Connect the microporous aeration plate to the aeration pipe, and connect the regulating valve on the aeration pipe to adjust the aeration rate, put it in a 20L primary culture tank, and use 75% concentration alcohol to treat the primary culture tank, Disinfect the aeration pan and aeration tube, and bind the mouth of the bucket with gauze and glass rope, and disinfect the gauze;

[0035] (3) Add 3L of aerobic denitrifying bacteria liquid into the primary culture tank and add the prepared medium, add water to dilute to 20L, turn on the micro aerator for oxygenation and stirring, ...

Embodiment 2

[0040] Such as figure 1 As shown, a rapid on-site expansion method of aerobic denitrifying bacteria, culture conditions: temperature 25°C; pH=7.5; dissolved oxygen 5mg / L;

[0041] Including the following steps:

[0042] (1) The laboratory inoculates and expands the aerobic denitrifying bacteria liquid, as the aerobic denitrifying bacteria seed liquid for on-site expansion;

[0043] (2) Connect the microporous aeration plate to the aeration pipe, and connect the regulating valve on the aeration pipe to adjust the aeration rate, put it in a 20L primary culture tank, and use 75% concentration alcohol to treat the primary culture tank, Disinfect the aeration pan and aeration tube, and bind the mouth of the bucket with gauze and glass rope, and disinfect the gauze;

[0044] (3) Add 3L of aerobic denitrifying bacteria liquid into the primary culture tank and add the prepared medium, add water to dilute to 20L, turn on the micro aerator for oxygenation and stirring, and cultivate ...

Embodiment 3

[0050] Such as figure 1 As shown, a rapid on-site expansion method of aerobic denitrifying bacteria, culture conditions: temperature 25 ° C; pH = 7.5; dissolved oxygen 6mg / L;

[0051] Including the following steps:

[0052] (1) The laboratory inoculates and expands the aerobic denitrifying bacteria liquid, as the aerobic denitrifying bacteria seed liquid for on-site expansion;

[0053] (2) Connect the microporous aeration plate to the aeration pipe, and connect the regulating valve on the aeration pipe to adjust the aeration rate, put it in a 20L primary culture tank, and use 75% concentration alcohol to treat the primary culture tank, Disinfect the aeration pan and aeration tube, and bind the mouth of the bucket with gauze and glass rope, and disinfect the gauze;

[0054] (3) Add 3L of aerobic denitrifying bacteria liquid into the primary culture tank and add the prepared medium, add water to dilute to 20L, turn on the micro aerator for oxygenation and stirring, and cultivat...

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Abstract

The invention belongs to the technical field of microbial culture and discloses an on-site rapid culture expansion method of aerobic denitrifying bacteria. The method comprises the following steps of(1) adding an aerobic denitrifying bacterial liquid into a first-stage culture tank, adding a culture medium, adding water for dilution, and carrying out oxygenation and stirring for culturing to obtain a bacterial liquid;(2) disinfecting two second-stage culture tanks, diluting each culture tank with water, and then performing oxygenation and stirring for culturing to obtain a bacterial liquid;(3) disinfecting two third-stage culture tanks, inoculating the bacterial liquid obtained in step (2) into one third-stage culture tank, filling the tank with a culture medium, adding water, and then performing oxygenation and stirring for culturing to obtain a bacterial liquid;(4) transferring half of the bacterial liquid obtained in step (3) into the other third-stage culture tank, adding a culture medium and water, and then performing oxygenation and stirring for culturing to obtain 2 tons of aerobic denitrifying bacterial liquid; (5) repeating steps (3) and (4) to obtain the ton-grade bacterial liquid according to demands. The culture expansion method has the advantages of simple operation, small investment and high practicability.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and relates to a rapid on-site expansion cultivation method of aerobic denitrifying bacteria. Background technique [0002] Aerobic denitrifying bacteria are a type of denitrifying bacteria that use the action of aerobic denitrifying enzymes to perform denitrification under aerobic conditions. Nitrifying bacteria mainly exist in Pseudomonas, Alcaligenes, Paracoccus and Bacillus. Since the microbial agent must maintain the effective number of viable bacteria and the activity of the bacteria when it is applied, but the project situation is changing at any time and the dosage of the microbial agent is continuous. If it is produced and packaged in the agent factory and shipped to the site, it will incur freight costs. High project cost and long on-site storage time increase the project cost and affect the quality of bacteria. Contents of the invention [0003] The technical prob...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 窦建军李雯蒋克梅刘光友
Owner 沐联环境科技(重庆)股份有限公司
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