67 results about "Microbial culture technique" patented technology

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention provides a method for promoting generation of tea acid from Nemania sp YAFEF112, and relates to the technical field of microbial culture. According to the method, mycelia of Nemania sp YAFEF112 are inoculated into a culture medium containing pruned walnut branches for culture, the culture medium is an MM culture medium, and the specific formula of the culture medium comprises 1.52 g / L of monopotassium phosphate, 0.52 g / L of magnesium sulfate, 6 g / L of sodium nitrate and 0.52 g / L of potassium chloride. According to the method, the defects in the prior art are overcome, and good conditions are provided for the production of the tea pickled acid by optimizing the formula of the fermentation culture medium, adding pruned walnut branches into the culture medium, improving the fermentation conditions and increasing the content of the tea pickled acid in the Nemania sp YAFEF112 hyphae.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention belongs to the technical field of microbial culture, and particularly relates to a culture medium composition and application thereof in PDRN recombinant escherichia coli fermentation. The culture medium composition comprises a fermentation culture medium and a fed-batch culture medium, the fermentation culture medium consists of disodium hydrogen phosphate, monopotassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate tetrahydrate, glycerol and water; the supplemented culture medium is composed of dipotassium phosphate, sodium dihydrogen phosphate, sodium sulfate, ammonium sulfate, ammonium chloride, diammonium hydrogen citrate, glycerin and water, and the metabolic requirements of PDRN recombinant escherichia coli are accurately matched. Meanwhile, the invention provides a fermentation method using the composition, and the problems of unbalanced nutrition, low fermentation efficiency and the like of an existing culture medium are solved. Stable proliferation of thalli can be remarkably promoted, the yield of PDRN products is increased, the culture medium is easy and convenient to prepare, fermentation conditions are easy to control, and the culture medium is suitable for industrial large-scale production and has important application value.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

The invention provides a microorganism composite culture device and method, and belongs to the technical field of microorganism culture.The microorganism composite culture device comprises a frame, an algae pool, a microorganism culture pool and an algae-eating insect culture pool are sequentially arranged in the frame from inside to outside, a center cylinder is arranged in the middle of the algae pool, and a mounting partition plate is arranged in the lower end of the center cylinder; a water conveying device is arranged in the middle of the installation partition plate, the water pumping end of the water conveying device is located on the lower portion, the water drainage end of the water conveying device is located in the algae pond on the upper portion, the algae pond and the microorganism culture pond are separated through a first partition plate, a sunlight shed is arranged at the upper end of the first partition plate, and the microorganism culture pond and the algae-eating insect culture pond are separated through a second partition plate. A carrier frame is arranged in the microorganism culture pond; the algae pool, the microorganism culture pool and the algae-eating insect culture pool are distributed in the frame body, so that algae, bacterial microorganisms, corophium and algae-eating insects can be subjected to co-culture, and a compound microorganism culture system is constructed.

The utility model relates to the technical field of saline-alkali soil microorganism culture, in particular to a storage device for saline-alkali soil microorganism culture, which comprises a storage box, a plurality of storage grooves are embedded in the top of the storage box, a plurality of culture cups are placed in the storage grooves, and a storage cavity and a lifting groove are respectively arranged on two sides of each culture cup. A sealing sliding plate is arranged in the storage cavity in a sealing and sliding fit mode, a sliding column which is in vertical sliding fit with the culture cup and can penetrate through the lifting groove is connected to the sealing sliding plate, the outer wall of the culture cup is provided with an inner concave surface and an annular groove which are communicated with each other, and a positioning opening is formed in the annular groove; according to the saline-alkali soil microorganism extraction device, microorganisms can be conveniently extracted under the condition that the soil structure of saline-alkali soil is not damaged, and meanwhile the saline-alkali soil microorganism extraction device is convenient and fast to use.

The utility model discloses an experimental device for ultraviolet mutagenesis of fungi, which belongs to the technical field of microbial culture and comprises an experimental cabinet main body, a movable cabinet door is rotatably connected to the outer end of the experimental cabinet main body, four uniformly distributed ultraviolet irradiation lamps are mounted at the top end in the experimental cabinet main body, and two lifting strips are fixedly connected to the left inner wall and the right inner wall of the experimental cabinet main body respectively. A through hole is formed in the outer end of the lifting block, a positioning block is slidably connected into the through hole, a connecting plate is fixedly connected into the lifting block and located on the outer side of the positioning block, a threaded rod is connected into the connecting plate in a threaded mode, and the threaded rod is located at the outer end of the positioning block and rotationally connected with the positioning block. A plurality of positioning grooves which are uniformly distributed and matched with the positioning blocks are formed in the outer end of the lifting strip, so that the experiment space layout can be flexibly adjusted by adjusting the positions of the lifting blocks on the lifting strip, and the space utilization rate is improved.

The application discloses a culture dish filling device and a use method, relates to the technical field of microbial culture, and solves the problems of complex operation, high use cost and high pollution risk of the existing culture medium filling mode, reduces the operation difficulty and the pollution risk, and specifically has the following arrangement: the device comprises a filling module and a filling bag hung on the filling module, the filling module is composed of a driving unit and a supporting block connected with the driving unit through a telescopic rod, the filling bag is hung on the supporting block, a rotating part is arranged on the supporting block, the rotating part is driven by a rotating motor arranged in the supporting block, a rotating switch is arranged on a filling head at the bottom of the filling bag, and the rotating switch is hung on the rotating part.

The invention relates to the technical field of microbial culture, in particular to an efficient tremella aurantialba liquid strain culture medium and a preparation and culture method. The feed comprises the following components in percentage by mass: 0.5% of corn flour; 3% of glucose; 0.4% of peptone; 0.2% of monopotassium phosphate; 0.1% of magnesium sulfate; 0.001% of vitamin B; and the balance of water. The method has the beneficial effects that 1, the growth efficiency is obviously improved; 2, the strain quality is stable and controllable; 3, the advantage of production cost is outstanding; operation is easy and convenient, and popularization is easy.

The utility model provides a quantitative strain ball dropping device, which relates to the technical field of microbial culture and comprises a liquid dropping mechanism, a liquid dropping tray, a moving mechanism and a base plate, the liquid dropping mechanism is mounted on the base plate, the moving mechanism is mounted on the base plate and positioned below the liquid dropping mechanism, the liquid dropping tray is mounted on the moving mechanism, and the moving mechanism is positioned below the liquid dropping tray. The liquid dropping disc is used for containing a plurality of independent containers, the movement direction of the liquid dropping mechanism is perpendicular to the movement direction of the moving mechanism, and the liquid dropping mechanism and the moving mechanism move in a crossed mode to ensure that quantitative strains are dropped into all the independent containers in the liquid dropping disc. According to the utility model, the small balls are prevented from absorbing moisture, shrinking or adhering together in the sub-packaging process, and the beneficial effects of short sub-packaging time and convenience in sub-packaging are achieved.

The invention provides a soil microorganism culture device and method, and belongs to the technical field of microorganism culture, the soil microorganism culture device comprises: a culture medium table serving as a mounting support for biological culture, a plurality of base table through holes being formed in the culture medium table, and the base table through holes being used for discharging culture soil in a culture cylinder; a material taking opening is formed in the side wall of the culture cylinder, and a material taking mechanism is fixedly installed outside the material taking opening and used for sampling microorganisms cultured at different depths in the culture cylinder; a liquid adding mechanism is fixedly arranged on the inner wall of the side, away from the material taking mechanism, of the culture cylinder, and the liquid adding mechanism is used for adding a nutrient solution to different depths of microorganism culture soil in the culture cylinder; by arranging the material taking mechanism and the liquid adding mechanism, sampling and nutrient solution adding can be carried out on microorganisms at different depths under the condition that the soil structure is not damaged.

The utility model discloses an intestinal anaerobic microorganism separation culture device, which belongs to the technical field of microorganism culture and comprises a sealed glove box, a first air inlet structure is arranged on one side of the sealed glove box, and a first air outlet structure is correspondingly arranged at one end, far away from the first air inlet structure, of the sealed glove box; a purifying and deoxidizing tank is arranged in the sealed glove box, and a deoxidant is arranged in the purifying and deoxidizing tank. According to the utility model, the content of oxygen in the sealed glove box is further reduced through inert gas replacement and subsequent deoxygenation of a deoxidant, a better anaerobic microorganism separation culture environment is created, and growth and reproduction of anaerobic microorganisms are facilitated.

This invention discloses a stirred photobioreactor with an integrated LED light source, relating to the field of photosynthetic microbial culture technology. The reactor includes a tank with a compressed air and carbon dioxide mixture inlet on the lower wall of the tank. The mixed gas is introduced into the bottom of the tank through the inlet. The top of the tank has a test port, a liquid inlet, and an exhaust port. The test port can be used to detect pH and dissolved oxygen levels. The reactor also includes a stirring assembly, a suspended lamp holder, and an LED light. This invention achieves 360° rotation by designing a suspended, integrated six-sided LED light fixture inside the tank. o The directional lighting is achieved by using a stirring impeller between the intervals of the suspended six-sided LED lamp tube bracket to reduce the amount of CO2 overflow during the air-lift stirring process. This can achieve the purpose of thorough mixing, reducing foam, preventing cell floating and adhesion to the wall, avoiding light blockage, and improving light energy utilization efficiency.

The application relates to the technical field of microbial culture, and discloses a honeycomb type microbial culture and mixing control method and device, which comprises the following steps: setting a distributed temperature sensor and a pressure sensor, collecting microbial colony image data, determining the growth size and maturity degree of the microorganisms, analyzing the growth size and maturity degree of the microorganisms, collecting the temperature of the microbial culture, judging whether the temperature of the microbial culture is abnormal, synchronously collecting the circulating water temperature in a preset period when it is judged that the temperature of the microbial culture is abnormal, judging whether the circulating water temperature for microbial culture incubation is abnormal, and adjusting the circulating water temperature when it is judged that the circulating water temperature is abnormal. The application improves the fine control requirement of the microorganisms and makes the control of the microbial culture more intelligent.

The invention relates to the technical field of microorganism culture, in particular to an efficient algae culture integrated device capable of independently adjusting light and temperature and a culture method thereof.The efficient algae culture integrated device comprises a heat conduction substrate and a culture module arranged on the top face of the heat conduction substrate, the culture module comprises a plurality of independent culture grooves distributed in a longitudinal and transverse array mode, and each culture groove forms an independent culture point location; the culture grooves are longitudinally arranged in the length direction of the heat conduction substrate, and an LED lamp strip capable of being independently controlled is embedded in the bottom of each culture groove; a heating medium circulation side and a refrigerant circulation side are arranged at the two ends of the heat conduction substrate respectively, a temperature gradient is formed by natural heat conduction from the heating medium circulation side to the refrigerant circulation side through the heat conduction substrate, and different temperature-illumination combined culture units are integrated at each culture point position. Uniformity and stability of illumination and temperature environment of each culture unit are achieved, the edge effect of illumination is eliminated, and independence, stability, accuracy and repeatability of experimental data are ensured. Meanwhile, system expansion and maintenance are facilitated, and the testing cost of a single sample is reduced.

The invention discloses a boletus aereus solid strain culture method, and relates to the technical field of microbial culture, the method comprises the following steps: preparing a special culture medium taking hard broadleaf sawdust with specific particle size and corncob as main materials, wheat bran and soybean meal as auxiliary materials, and mulberry twig fermentation dry powder as a biological activity induction component; putting the special solid culture medium into a culture bottle, sealing, sterilizing, and cooling for later use; inoculating the boletus aereus mother strain into the sterilized special solid culture medium; a micro-positive pressure intermittent ventilation culture process which is closed in the whole process and does not need punching is adopted for the culture bottle after culture and inoculation. The invention aims to solve the problems of high pollution risk, slow hypha growth and high planting failure rate caused by manual punching due to compact structure and poor air permeability of the existing solid culture gene, realizes the cooperation of high-porosity oxygen supply, precise nutrition supply and sterile environment guarantee, and remarkably improves the hypha activity and culture success rate. The method is suitable for efficient industrial production of boletus aereus solid strains.

This invention relates to the field of microbial culture technology, and more specifically, to a microbial fermentation culture tank for planting culture media. The tank includes a tank body, a motor fixedly mounted on the top of the tank cover, a stirring shaft fixedly connected to the bottom of the motor drive shaft, and a stirring paddle fixedly mounted on the outer side of the middle section of the stirring shaft. The invention is characterized by: a defoaming component positioned between the outer top of the stirring shaft and the inner wall of the tank; a scraper ring component positioned at the top of the defoaming component; and a homogenizing component positioned between the bottom of the defoaming component and the bottom of the stirring shaft. This invention uses a rotating stirring shaft to drive the defoaming paddle and collision blocks to rotate, causing the collision plates to collide with the collision blocks. This, in turn, causes the scraper ring to rotate and slide, scraping away the fermentation liquid on the inner wall. Simultaneously, the bottom stirring teeth can perform horizontal rotational stirring and linear reciprocating stirring perpendicular to the stirring shaft, resulting in a more uniform mixing density of the fermentation liquid at the bottom.

The application provides a kind of liquid culture medium for cultivating high-efficiency composite microbial system for compost and application thereof, and belongs to the technical field of microbial culture;The liquid culture medium includes microbial culture base medium and the following components: organic solid waste, self-inducer-2 (AI-2), N-acyl homoserine lactone (AHLs), vitamin B1, biotin and L-cysteine;The liquid culture medium takes organic solid waste, the target degradable substance, as carbon source;AI-2 and AHLs are used as signal molecules;Vitamin B1, biotin and L-cysteine are used as growth factors;Through the synergistic effect of characteristic carbon source, signal molecule and growth factor in microbial culture medium, the cultivation of high-efficiency composite microbial system for compost containing unculturable microorganisms in inoculum is realized, the number of available functional strains in organic solid waste composting system is greatly enriched and improved, and the interaction and degradation efficiency are strengthened.

The application discloses a fermentation culture method of Bacillus thuringiensis Bt64 strain and belongs to the technical field of microbial culture. The orthogonal test is adopted to optimize the culture medium components of the Bacillus thuringiensis Bt64 strain, high-quality and low-cost culture medium with corn powder, corn starch and potato starch as carbon sources and soybean powder and fish meal as nitrogen sources is determined, and the optimal component ratio is determined. Compared with the LB culture medium, the Bt64 strain cultured by using the culture medium is amplified fast and declines slowly. Based on the culture medium, the temperature, inoculation ratio, time, ventilation amount and rotating speed of the fermentation culture of the Bt64 strain are further optimized, and the optimal culture conditions suitable for the rapid and large-scale proliferation of the Bt64 strain are determined. The application provides a reference basis and technical support for the fermentation tank culture, industrial development and large-scale production of the Bacillus thuringiensis Bt64 strain.

The present application belongs to the technical field of microbial culture, and discloses a microbial culture device with the function of improving microbial activity; the device comprises a culture device body and a tank body, and further comprises: a rotor for uniformly distributing oxygen in the tank body, the rotor being rotatably arranged in the tank body; and a multi-element ion sterilization device for releasing multiple active ions, different active ions being capable of inhibiting non-target bacteria and promoting target bacteria to metabolize, the multi-element ion sterilization device being arranged in the rotor. The present application is provided with the multi-element ion sterilization device and a self-feedback ion grid, oxygen is input into the rotor cavity through a T-shaped pipe, is transported to an annular gap through a spiral pipe, and is distributed by centrifugal force when the rotor rotates, gas-liquid exchange membranes realize efficient oxygen dissolution, the multi-element ion sterilization device releases ions to inhibit miscellaneous bacteria and competitive bacteria, and is switched to a nutrient ion release mode to promote target bacteria to metabolize, and the ion grid adsorbs or releases ions through an electrochemical sensor to maintain concentration balance.

The invention relates to the technical field of microbial culture, in particular to a strain morphology screening system and method based on an image technology. The method comprises the following steps: diluting a to-be-screened strain to a predetermined cell concentration, and injecting the diluted strain into a micro-chamber array of a micro-fluidic chip; periodically collecting images of the micro-cavity array, measuring morphological parameters of cells in each micro-cavity, and recording a collection moment; calculating the variable quantity of each morphological parameter of the same micro-chamber between two adjacent acquisition moments, performing weighted summation on the variable quantity of each morphological parameter according to a preset weight, and dividing the sum by the time interval to obtain the morphological change speed of each micro-chamber; and when the morphological change speed of the micro-chamber exceeds a preset speed threshold, adding the micro-chamber to a key monitoring list. According to the invention, the micro-chamber array of the micro-fluidic chip is combined with morphological parameter extraction and machine learning classification, and dynamic monitoring and accurate identification are carried out on the strain morphological transformation process, so that efficient screening of target strains is realized.

The utility model discloses a microorganism culture device for bioengineering teaching, which belongs to the technical field of microorganism culture, and comprises a biological cabinet and a culture dish, a flow guide cover is arranged above the culture dish, the inner wall of the biological cabinet is connected with two water tanks, the inner sides of the water tanks are connected with drainage pipes, and the drainage pipes are connected with the biological cabinet. One end of the drainage pipe is connected with a water storage tank, the water storage tank is connected with the inner bottom wall of the biological cabinet, the flow guide cover, the water tank and the drainage pipe are matched, water vapor is blocked through the flow guide cover, the water vapor is blocked on the flow guide cover to be condensed into water drops, and the water drops are guided to flow to the water tank through the inclination angle of the flow guide cover; water drops are uniformly collected through the water tank and prevented from flowing to the outside, the water drops flow into the water storage tank through the drainage pipe in the water tank, the water drops are concentrated through the water storage tank, contact between water vapor and the culture dishes is reduced, the water drops carried when the culture dishes are taken out are reduced, and the dryness of the teaching field is improved.

The invention relates to the technical field of microbial culture, in particular to combined antibiotic adsorption resin and application thereof. The combined antibiotic adsorption resin obtained through screening can achieve a good adsorption effect on clinically common antibiotics, is good in biocompatibility, and can well adsorb antibiotics existing in a system while ensuring microbial growth.

The invention relates to the field of microbial culture, discloses a culture method for promoting growth of mucus type pseudomonas aeruginosa by using IgG, and belongs to the technical field of microbial culture. According to the method, a specific improved system is constructed aiming at the technical problems that mucus type pseudomonas aeruginosa with the genotype of ST270 is too long in growth retardation period and extremely slow in proliferation in a conventional LB or TSB culture medium. The method comprises the following specific steps: preparing a basic liquid culture medium and sterilizing; non-thermally inactivated immunoglobulin G (IgG) is added in a sterile manner, so that the final concentration of the non-thermally inactivated immunoglobulin G (IgG) is in a range of 5.0 mu g / mL to 5.0 mg / mL; and inoculating a target strain and then carrying out shake culture at 37 DEG C. By utilizing the modification effect of IgG molecules on the mucus layer on the surface of the thallus, physical steric hindrance or metabolic inhibition caused by an extracellular matrix is effectively overcome, the growth adaptation period is remarkably shortened, the biomass of the thallus is greatly improved, and rapid amplification and phenotype recovery of the rare strains difficult to culture are successfully realized.

The invention belongs to the technical field of microbe culture, and particularly relates to a far infrared heat preservation culture container which comprises a movable base and a microbe culture tank fixedly arranged at the top of the movable base through a support, an air inlet assembly is connected to the outer side of the bottom end of the microbe culture tank in a penetrating mode, and a supporting base column is fixedly arranged at the bottom of an inner cavity of the microbe culture tank. Hot air can be used for carrying out auxiliary heating on a culture solution, so that heating is more uniform, the temperature of the culture solution at different positions in the microorganism culture tank can be detected through a temperature sensor, and when the temperature of the culture solution at the corresponding area is too low and does not reach the normal temperature, the culture solution is heated. Gas to be blown out can be secondarily heated through a grating type heating plate in a heating type lifting disc, the gas is sprayed out from a first nozzle and a second nozzle after being secondarily heated, culture solutions in corresponding areas can be subjected to compensation type heating, the temperatures of the culture solutions in different positions can be made to be the same, and therefore the culture solutions can be heated more accurately. And the heating uniformity can be further improved.

The invention discloses a probiotic culture system, and relates to the technical field of microbial culture, the probiotic culture system comprises a culture tank, the bottom of the culture tank is provided with a fixed bottom plate, the top of the culture tank is in lap joint with a sealing cover, the top of the sealing cover is provided with a driving assembly, the bottom of the sealing cover is rotatably connected with a mounting disc, and the bottom of the mounting disc is provided with a movable rod; a stirring rod is mounted on the surface of the movable rod; according to the probiotic culture system, the control assembly is configured to execute the preset programmed culture process, and the arrangement of the driving assembly, the injection pipe and the discharge pipe is cooperatively controlled on the basis of feedback data of the sensor assembly, so that the system can automatically and accurately adjust the stirring strength and the material adding strategy according to the real-time culture environment (such as pH and dissolved oxygen); and harvesting is automatically triggered when conditions are met, so that closed-loop automatic control of the whole process from sterilization, culture to harvesting is realized, the consistency and repeatability of the process are improved, and errors and pollution risks caused by human intervention are reduced.

The invention discloses a selective culture medium and method for screening or separating aeromonas, and relates to the technical field of microbial culture. The selective culture medium comprises a yeast extract, a nitrogen source, a carbon source, sodium citrate, sodium deoxycholate, sodium thiosulfate, ferric citrate, sodium chloride, agar, an acid-base indicator, ampicillin and diaminopteridine. The method can be used for rapidly and efficiently separating the aeromonas, and provides reliable preposition selection for detection or identification of the aeromonas.

The invention discloses a culture medium for rapidly culturing thiobacillus denitrificans as well as a preparation method and application of the culture medium, and belongs to the technical field of microbial culture. Aiming at the chemical energy autotrophic metabolism characteristics of thiobacillus denitrificans, a highly adaptive, stable and efficient growth environment is cooperatively constructed through precise formula regulation and optimized step-by-step preparation and classified sterilization processes. The culture medium disclosed by the invention can remarkably shorten the growth delay phase of thalli, promote the thalli to quickly enter the logarithmic phase and realize high-density and high-activity stable culture, and the cultured thiobacillus denitrificans show excellent denitrification performance in sewage treatment. The culture medium provided by the invention systematically solves the core problems of long culture period, low efficiency, high cost, poor large-scale stability and the like in the prior art, and has a good application prospect.

This invention relates to the field of microbial culture technology, and particularly to a microbial culture device for industrial wastewater treatment. The device includes a reaction tank, an aerator slidably connected inside the reaction tank, and a stirring assembly inside the reaction tank. The stirring assembly includes a fixed plate, one side of which is fixedly connected to the top of the reaction tank. A motor is fixedly connected inside the fixed plate, and a transmission rod is fixedly connected to the output end of the motor. Symmetrical disassembly components are provided on the outer wall of the aerator. This invention uses a motor to drive the transmission rod, reducing the resistance of the stirring rod during stirring through perforations and increasing liquid flow. The movement of the transmission rod further drives the connecting rod to move synchronously, thereby achieving the effect of quickly scraping away residues on the inner wall of the reaction tank. This solves the problems of reduced effective volume, uneven mixing, and increased energy consumption caused by residue adhesion, improving wastewater treatment efficiency and equipment operational stability.