121 results about "Microbial culture technique" patented technology

The invention relates to the technical field of microorganism culture, and discloses a microorganism culture tank device which comprises a culture tank, a concave observation area is arranged in the center of the top of the culture tank, a central culture area surrounding the observation area is arranged in the culture tank, an oil path temperature control area wraps the culture tank, and a double-helix heat conduction oil path is integrated in the oil path temperature control area. Through the cooperation of the uniform distributor and the distributed simulated liquid conveying unit, the gas-liquid proportion and fluid distribution can be accurately regulated and controlled, the physical and chemical conditions in the deep sea or extreme environment are simulated, the constant-temperature precision of + / -0.005 DEG C is realized through the double-helix heat conduction oil way, the low-temperature environment is stably maintained, and the real thermodynamic conditions of deep-sea sediments are simulated; the device meets the sensitive requirements of different microorganisms on temperature, and has the characteristics of high practicability and capability of simulating a dynamic fluid environment of deep sea.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention provides a method for promoting generation of tea acid from Nemania sp YAFEF112, and relates to the technical field of microbial culture. According to the method, mycelia of Nemania sp YAFEF112 are inoculated into a culture medium containing pruned walnut branches for culture, the culture medium is an MM culture medium, and the specific formula of the culture medium comprises 1.52 g / L of monopotassium phosphate, 0.52 g / L of magnesium sulfate, 6 g / L of sodium nitrate and 0.52 g / L of potassium chloride. According to the method, the defects in the prior art are overcome, and good conditions are provided for the production of the tea pickled acid by optimizing the formula of the fermentation culture medium, adding pruned walnut branches into the culture medium, improving the fermentation conditions and increasing the content of the tea pickled acid in the Nemania sp YAFEF112 hyphae.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention relates to the technical field of endoscopic microbial culture, and discloses a dynamic perfusion control system and method for an endoscopic microbial culture agent, and the system comprises a multi-channel data collection module which is used for obtaining multi-dimensional culture agent parameters including concentration distribution, perfusion rate and metabolite in real time, wherein the concentration distribution data comprises first environment distribution data generated by the multi-region perfusion device and second dynamic feedback data collected by the optical sensor array; the dynamic regulation and control module performs gradient equilibrium correction processing on the parameters and inputs the parameters into the perfusion analysis processing layer for feature modeling, the perfusion analysis processing layer comprises a data preprocessing module and an environment modeling module, and the environment modeling module comprises an environment parameter fusion layer, a gradient equilibrium layer and a perfusion decision-making layer which are connected in sequence. And finally, generating a perfusion regulation and control instruction according to an output result. According to the invention, real-time monitoring and dynamic accurate regulation and control of multi-dimensional parameters are realized, and the quality and efficiency of endoscopic microorganism culture are improved.

The invention belongs to the technical field of microbial culture, and particularly relates to a culture medium composition and application thereof in PDRN recombinant escherichia coli fermentation. The culture medium composition comprises a fermentation culture medium and a fed-batch culture medium, the fermentation culture medium consists of disodium hydrogen phosphate, monopotassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate tetrahydrate, glycerol and water; the supplemented culture medium is composed of dipotassium phosphate, sodium dihydrogen phosphate, sodium sulfate, ammonium sulfate, ammonium chloride, diammonium hydrogen citrate, glycerin and water, and the metabolic requirements of PDRN recombinant escherichia coli are accurately matched. Meanwhile, the invention provides a fermentation method using the composition, and the problems of unbalanced nutrition, low fermentation efficiency and the like of an existing culture medium are solved. Stable proliferation of thalli can be remarkably promoted, the yield of PDRN products is increased, the culture medium is easy and convenient to prepare, fermentation conditions are easy to control, and the culture medium is suitable for industrial large-scale production and has important application value.

The invention relates to the technical field of microorganism culture, in particular to a planting culture medium microorganism fermentation culture tank which comprises a tank body, a motor fixedly installed at the top end of a tank cover on the upper portion of the tank body, a stirring shaft fixedly connected to the bottom end of a motor driving shaft and a stirring paddle fixedly installed on the outer side of the middle of the stirring shaft. The device is characterized in that a defoaming assembly is arranged between the outer side of the top of the stirring shaft and the inner wall of the tank body, a scraping ring assembly is arranged at the top of the defoaming assembly, and a homogenizing assembly is arranged between the bottom end of the defoaming assembly and the bottom of the stirring shaft. The rotating stirring shaft drives the defoaming paddle and the collision block to rotate, so that the collision plate collides with the collision block, then the scraping ring is driven to rotate and slide to scrape fermentation liquor on the inner wall, and meanwhile, the stirring teeth at the bottom can perform horizontal rotary stirring and linear reciprocating stirring perpendicular to the stirring shaft; and the mixing density of bottom fermentation liquor is more uniform.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

The embodiment of the utility model discloses a microorganism expanding culture device which comprises a microorganism culture bin, a filler unit and an aeration unit, the side wall of the microorganism culture bin is provided with a feed port, a dosing port and a discharge port, and the feed port and the dosing port are respectively used for inputting reaction raw water and nutrient substances into the microorganism culture bin; the discharge port is used for discharging reacted water in the microorganism culture bin to the outside; the filler unit is arranged in the microorganism culture bin, and the filler unit is used for attachment growth of microorganisms; the aeration unit is arranged in the microorganism culture bin and is positioned at the bottom of the filler unit; the aeration unit is used for providing oxygen for microorganism breeding in the microorganism culture bin. The microorganism expanding culture device adopts a mud-film symbiotic method microorganism culture technology to promote microorganisms to be in an efficient propagation state all the time, so that continuous and efficient microorganism amplification is realized.

The invention discloses a silicon rubber surface green algae adhesion layer cultivation method and a cultivation device, the method comprises algae concentration, surface pretreatment, spraying treatment, green algae inoculation and cultivation, the cultivation method specifically comprises the following steps: placing an inoculated silicon rubber sample in the cultivation device, controlling the temperature to be 25-30 DEG C, the humidity to be 70-90%, the illumination period to be 12 h / 12 h, the illumination intensity to be 100-200 mol.m <-2 >. S <-1 >, and the illumination time to be 12 h / 12 h; and continuously supplying the culture solution through the hanging type dropper to maintain the growth of the green algae, and continuously supplying the culture solution through the hanging type dropper to maintain the growth of the green algae. The method can simulate light, temperature and gas conditions in a natural environment, and realizes period-controllable and density-controllable green alga adhesion layer construction in a laboratory environment by combining a microbial culture technology, so that the rule of influence of green alga adhesion on the performance of the composite insulator is more accurately mastered; scientific basis and technical support are provided for prevention and treatment of external insulation biological contamination of an electric power system.

The invention provides a microorganism composite culture device and method, and belongs to the technical field of microorganism culture.The microorganism composite culture device comprises a frame, an algae pool, a microorganism culture pool and an algae-eating insect culture pool are sequentially arranged in the frame from inside to outside, a center cylinder is arranged in the middle of the algae pool, and a mounting partition plate is arranged in the lower end of the center cylinder; a water conveying device is arranged in the middle of the installation partition plate, the water pumping end of the water conveying device is located on the lower portion, the water drainage end of the water conveying device is located in the algae pond on the upper portion, the algae pond and the microorganism culture pond are separated through a first partition plate, a sunlight shed is arranged at the upper end of the first partition plate, and the microorganism culture pond and the algae-eating insect culture pond are separated through a second partition plate. A carrier frame is arranged in the microorganism culture pond; the algae pool, the microorganism culture pool and the algae-eating insect culture pool are distributed in the frame body, so that algae, bacterial microorganisms, corophium and algae-eating insects can be subjected to co-culture, and a compound microorganism culture system is constructed.

The utility model relates to the technical field of saline-alkali soil microorganism culture, in particular to a storage device for saline-alkali soil microorganism culture, which comprises a storage box, a plurality of storage grooves are embedded in the top of the storage box, a plurality of culture cups are placed in the storage grooves, and a storage cavity and a lifting groove are respectively arranged on two sides of each culture cup. A sealing sliding plate is arranged in the storage cavity in a sealing and sliding fit mode, a sliding column which is in vertical sliding fit with the culture cup and can penetrate through the lifting groove is connected to the sealing sliding plate, the outer wall of the culture cup is provided with an inner concave surface and an annular groove which are communicated with each other, and a positioning opening is formed in the annular groove; according to the saline-alkali soil microorganism extraction device, microorganisms can be conveniently extracted under the condition that the soil structure of saline-alkali soil is not damaged, and meanwhile the saline-alkali soil microorganism extraction device is convenient and fast to use.

The present invention discloses a microbial culture storage device and a culture storage method, belonging to the technical field of microbial culture. The microbial culture storage device includes a box body, and a clamping mechanism for clamping culture dishes is arranged inside the box body. The clamping mechanism includes a mounting plate, a plurality of card slots for placing culture dishes are arranged on the mounting plate, a clamping structure is arranged on the side wall of the card slot, and a transmission structure for driving the clamping structure to act is arranged between the fixing plate in the incubator and the mounting plate; a stirring structure for stirring the culture solution and performing ventilation is arranged inside the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged inside the box body. By using the microbial culture storage device and the culture storage method of the present invention, the problems that it is not easy to detect metabolic substances during the process of microbial culture using culture dishes and the culture dishes are not stably fixed can be solved.

The invention relates to the technical field of microorganism culture, in particular to a ganoderma sinensis spore germination culture medium and application thereof.Ganoderma sinensis spores are collected through a fungus carrying bag or the culture medium, the optimal growth environment for ganoderma sinensis spore germination culture is obtained by optimizing the culture condition of the ganoderma sinensis spores, and the ganoderma sinensis spore germination culture medium is obtained by optimizing the culture medium formula and adding wood chip boiling liquid. A culture medium formula most suitable for ganoderma spore germination is obtained through raw material screening and orthogonal experiment optimization of boiling liquid, ganoderma spores can be effectively collected without reaching a sterile environment of a laboratory, the method is a practical and effective ganoderma sinensis spore collection and germination method, and a foundation is laid for promoting ganoderma sinensis new variety breeding research.

The utility model discloses an experimental device for ultraviolet mutagenesis of fungi, which belongs to the technical field of microbial culture and comprises an experimental cabinet main body, a movable cabinet door is rotatably connected to the outer end of the experimental cabinet main body, four uniformly distributed ultraviolet irradiation lamps are mounted at the top end in the experimental cabinet main body, and two lifting strips are fixedly connected to the left inner wall and the right inner wall of the experimental cabinet main body respectively. A through hole is formed in the outer end of the lifting block, a positioning block is slidably connected into the through hole, a connecting plate is fixedly connected into the lifting block and located on the outer side of the positioning block, a threaded rod is connected into the connecting plate in a threaded mode, and the threaded rod is located at the outer end of the positioning block and rotationally connected with the positioning block. A plurality of positioning grooves which are uniformly distributed and matched with the positioning blocks are formed in the outer end of the lifting strip, so that the experiment space layout can be flexibly adjusted by adjusting the positions of the lifting blocks on the lifting strip, and the space utilization rate is improved.

The application discloses a culture dish filling device and a use method, relates to the technical field of microbial culture, and solves the problems of complex operation, high use cost and high pollution risk of the existing culture medium filling mode, reduces the operation difficulty and the pollution risk, and specifically has the following arrangement: the device comprises a filling module and a filling bag hung on the filling module, the filling module is composed of a driving unit and a supporting block connected with the driving unit through a telescopic rod, the filling bag is hung on the supporting block, a rotating part is arranged on the supporting block, the rotating part is driven by a rotating motor arranged in the supporting block, a rotating switch is arranged on a filling head at the bottom of the filling bag, and the rotating switch is hung on the rotating part.

The present invention discloses a method for rapidly enriching and isolating purple non-sulfur bacteria from sewage treatment systems. This method optimizes the PNSB enrichment medium and uses infrared anaerobic methods to rapidly enrich high concentrations of PNSB from complex sewage environments. Simultaneously, a single PNSB strain is isolated using the optimized isolation medium and a simple light anaerobic device. The method provided by the present invention enables rapid enrichment and isolation of PNSB from complex environments, resulting in higher bacterial purity and simpler isolation methods, thus possessing important practical applications in microbial culture technology.

The culture medium comprises an incubator, the top of the incubator is provided with a detection unit for detecting the number of microorganisms in the incubator, and the top of the incubator is further provided with a quantitative feeding mechanism for quantitatively feeding a culture solution; the invention relates to the technical field of microbial culture. According to the mass microorganism culture medium, through the arrangement of the quantitative feeding mechanism and the driving assembly, automatic quantitative feeding of a culture solution is achieved, the culture solution is quantitatively fed and discharged once by pressing a pressing cylinder once, meanwhile, a rotating plate rotates by 90 degrees, and the quantitatively fed culture solution falls into a culture box through a fan-shaped groove; along with culture and reproduction of microorganisms, quantitative feeding is carried out multiple times, the amount of the culture solution is increased, the concentration and the amount of the culture solution are synchronously increased when the microorganisms are gradually increased, quantitative feeding facilitates statistics of feeding times, and therefore the concentration and the amount of the culture solution are calculated, and precise culture of the microorganisms is achieved.

The invention relates to the technical field of microbial culture, in particular to an efficient tremella aurantialba liquid strain culture medium and a preparation and culture method. The feed comprises the following components in percentage by mass: 0.5% of corn flour; 3% of glucose; 0.4% of peptone; 0.2% of monopotassium phosphate; 0.1% of magnesium sulfate; 0.001% of vitamin B; and the balance of water. The method has the beneficial effects that 1, the growth efficiency is obviously improved; 2, the strain quality is stable and controllable; 3, the advantage of production cost is outstanding; operation is easy and convenient, and popularization is easy.

The invention relates to the technical field of microbial culture, in particular to a method for increasing the titer of Klebsiella pneumoniae bacteriophage, which comprises the following steps: step 1, culturing host bacteria; step 2, regulation and control of infection complex number: setting a plurality of infection complex numbers of different bacteriophage-klebsiella pneumoniae number ratios, and standing to obtain a mixed solution; and step 3, lysis culture: carrying out shake culture on the adsorbed mixed solution for 1.5-2 hours until the solution becomes clear, observing the clarity change of the solution, filtering to obtain a bacteriophage suspension, and determining the preferable infection complex number and bacteriophage titer by measuring the bacteriophage suspension titer. According to the method, by optimizing host bacterium culture conditions and infection complex number regulation and control and combining a standardized splitting decomposition judgment method of light transmittance and visual colorimetry, under the condition that centrifugal concentration or advanced equipment is not needed, the titer of the klebsiella pneumoniae bacteriophage is remarkably improved, and efficient, low-cost, broad-spectrum and safe bacteriophage preparation is achieved.

The present invention relates to the technical field of microbial culture, and discloses a cell culture device capable of synchronously replacing culture medium, comprising: a box body, comprising a plate body and a surrounding plate, wherein the surrounding plate and the upper surface of the plate body form a main pool capable of accommodating culture medium; a base hole, which is opened on the upper plate surface of the plate body, and the base holes include a plurality of base holes, which are arranged in a matrix; a filter cartridge, wherein a filter cartridge is provided above each base hole, and a downstream flow gap is provided on the cartridge wall of the filter cartridge on at least the downstream side; a sinking cavity is provided on the upper plate surface of the plate body on the upstream side of each base hole, and an upstream hole wall is defined between the sinking cavity and the base hole, and a first upstream flow gap is provided on the upstream hole wall; a water dam is provided between each two adjacent filter cartridges, and adjacent rows of water dams and filter cartridges separate the main pool into pool units, and each row of water dams enables the culture medium in the pool unit to actively flow to the sinking cavity and enter the base hole through the first upstream flow gap.

The invention discloses a culture method for improving freeze-drying stress resistance and storage stress resistance of bifidobacterium and application of the culture method, and belongs to the technical field of microorganism culture. The method comprises the following steps: firstly, establishing a freeze-drying model of the pH dynamic regulation thalli for evaluating the influence of freeze-drying and storage activity of the thalli; based on the established freeze drying model of the pH dynamic regulation thalli, better culture conditions before freeze drying and better temperature during storage of the bifidobacterium are screened. According to the culture method for improving freeze-drying stress resistance and storage stress resistance of bifidobacteria and the application of the culture method, by regulating and controlling the active state of cultured thalli, the later-stage drying and storage stress resistance of strains is improved, and a foundation is provided for precise culture of probiotics and processing of active preparations.

The invention relates to the technical field of microbial culture, and discloses a biological cell culture device, which comprises: a culture main body, which is provided with a plurality of columnar cavities arranged in a matrix; the top assembly comprises a transferable module and a cover plate, the transferable module comprises a supporting sheet body and a plurality of filter baskets, the supporting sheet body is provided with a plurality of first positioning holes, the filter baskets are attached to the first positioning holes, the supporting sheet body covers the upper plate surface of the culture main body, and the cover plate covers the upper plate surface of the culture main body; the cover plate is covered on the supporting sheet body; the bottom component covers the bottom of the culture main body; the bottom assembly defines a plurality of fluid supply units, the fluid supply units are communicated with the columnar cavities respectively, each fluid supply unit comprises a liquid flow channel and a gas flow channel, the far ends of the liquid flow channels and the gas flow channels are connected to the columnar cavities, and the on-off states of the liquid flow channels and the gas flow channels are controlled at the near ends.

The present invention discloses a method for rapidly separating white rot fungi infected by miscellaneous fungi, belonging to the technical field of microbial culture. This method mainly includes four steps: spore liquid preparation, isolation and purification culture, identification culture, and scale-up culture. Different from traditional methods such as streak separation and addition of inhibitors, this method mainly conducts restrictive culture on common miscellaneous fungi such as Penicillium and Trichoderma in production applications through a white rot fungi selective medium, and rejuvenates the nutrition of the target strain. This method is simple to operate, has a high success rate, effectively reduces the time cost, and further improves the production efficiency. In addition, the white rot fungi separated and purified by this method have high activity and stable quality.

The utility model provides a quantitative strain ball dropping device, which relates to the technical field of microbial culture and comprises a liquid dropping mechanism, a liquid dropping tray, a moving mechanism and a base plate, the liquid dropping mechanism is mounted on the base plate, the moving mechanism is mounted on the base plate and positioned below the liquid dropping mechanism, the liquid dropping tray is mounted on the moving mechanism, and the moving mechanism is positioned below the liquid dropping tray. The liquid dropping disc is used for containing a plurality of independent containers, the movement direction of the liquid dropping mechanism is perpendicular to the movement direction of the moving mechanism, and the liquid dropping mechanism and the moving mechanism move in a crossed mode to ensure that quantitative strains are dropped into all the independent containers in the liquid dropping disc. According to the utility model, the small balls are prevented from absorbing moisture, shrinking or adhering together in the sub-packaging process, and the beneficial effects of short sub-packaging time and convenience in sub-packaging are achieved.

The invention provides a soil microorganism culture device and method, and belongs to the technical field of microorganism culture, the soil microorganism culture device comprises: a culture medium table serving as a mounting support for biological culture, a plurality of base table through holes being formed in the culture medium table, and the base table through holes being used for discharging culture soil in a culture cylinder; a material taking opening is formed in the side wall of the culture cylinder, and a material taking mechanism is fixedly installed outside the material taking opening and used for sampling microorganisms cultured at different depths in the culture cylinder; a liquid adding mechanism is fixedly arranged on the inner wall of the side, away from the material taking mechanism, of the culture cylinder, and the liquid adding mechanism is used for adding a nutrient solution to different depths of microorganism culture soil in the culture cylinder; by arranging the material taking mechanism and the liquid adding mechanism, sampling and nutrient solution adding can be carried out on microorganisms at different depths under the condition that the soil structure is not damaged.

The utility model discloses an intestinal anaerobic microorganism separation culture device, which belongs to the technical field of microorganism culture and comprises a sealed glove box, a first air inlet structure is arranged on one side of the sealed glove box, and a first air outlet structure is correspondingly arranged at one end, far away from the first air inlet structure, of the sealed glove box; a purifying and deoxidizing tank is arranged in the sealed glove box, and a deoxidant is arranged in the purifying and deoxidizing tank. According to the utility model, the content of oxygen in the sealed glove box is further reduced through inert gas replacement and subsequent deoxygenation of a deoxidant, a better anaerobic microorganism separation culture environment is created, and growth and reproduction of anaerobic microorganisms are facilitated.

The utility model discloses a brucellosis pathogen culture device, which belongs to the technical field of microorganism culture and comprises a culture box, two symmetrically distributed connecting frames are fixedly connected to the inner walls of the two sides of the culture box, a plurality of culture dishes are arranged between the two connecting frames, a transverse plate is fixedly connected to the tops of the two connecting frames, and the transverse plate is fixedly connected to the bottom of the culture box. Through the design of the height adjusting assembly, up-down movement of the culture dish in the incubator is achieved, the problem that the culture dish is inconvenient to take due to the fact that the height of the incubator is too high is effectively solved, an experimenter does not need to frequently stand on tiptoes or bend down or use tools such as a ladder, and the height of the culture dish can be easily adjusted only through simple operation of a PLC. The operation complexity is greatly reduced, through the design of a stable transmission system and a clamping plate, the safety and stability of the culture dish in the moving process are ensured, damage to the culture dish and leakage of pathogens are effectively avoided, and the reliability and safety of experiments are improved.

This invention discloses a stirred photobioreactor with an integrated LED light source, relating to the field of photosynthetic microbial culture technology. The reactor includes a tank with a compressed air and carbon dioxide mixture inlet on the lower wall of the tank. The mixed gas is introduced into the bottom of the tank through the inlet. The top of the tank has a test port, a liquid inlet, and an exhaust port. The test port can be used to detect pH and dissolved oxygen levels. The reactor also includes a stirring assembly, a suspended lamp holder, and an LED light. This invention achieves 360° rotation by designing a suspended, integrated six-sided LED light fixture inside the tank. o The directional lighting is achieved by using a stirring impeller between the intervals of the suspended six-sided LED lamp tube bracket to reduce the amount of CO2 overflow during the air-lift stirring process. This can achieve the purpose of thorough mixing, reducing foam, preventing cell floating and adhesion to the wall, avoiding light blockage, and improving light energy utilization efficiency.