10 results about "Microbial culture technique" patented technology

This invention relates to the field of microbial culture technology, and more specifically, to a microbial fermentation culture tank for planting culture media. The tank includes a tank body, a motor fixedly mounted on the top of the tank cover, a stirring shaft fixedly connected to the bottom of the motor drive shaft, and a stirring paddle fixedly mounted on the outer side of the middle section of the stirring shaft. The invention is characterized by: a defoaming component positioned between the outer top of the stirring shaft and the inner wall of the tank; a scraper ring component positioned at the top of the defoaming component; and a homogenizing component positioned between the bottom of the defoaming component and the bottom of the stirring shaft. This invention uses a rotating stirring shaft to drive the defoaming paddle and collision blocks to rotate, causing the collision plates to collide with the collision blocks. This, in turn, causes the scraper ring to rotate and slide, scraping away the fermentation liquid on the inner wall. Simultaneously, the bottom stirring teeth can perform horizontal rotational stirring and linear reciprocating stirring perpendicular to the stirring shaft, resulting in a more uniform mixing density of the fermentation liquid at the bottom.

This invention relates to the field of microbial culture technology, and particularly to a microbial culture device for industrial wastewater treatment. The device includes a reaction tank, an aerator slidably connected inside the reaction tank, and a stirring assembly inside the reaction tank. The stirring assembly includes a fixed plate, one side of which is fixedly connected to the top of the reaction tank. A motor is fixedly connected inside the fixed plate, and a transmission rod is fixedly connected to the output end of the motor. Symmetrical disassembly components are provided on the outer wall of the aerator. This invention uses a motor to drive the transmission rod, reducing the resistance of the stirring rod during stirring through perforations and increasing liquid flow. The movement of the transmission rod further drives the connecting rod to move synchronously, thereby achieving the effect of quickly scraping away residues on the inner wall of the reaction tank. This solves the problems of reduced effective volume, uneven mixing, and increased energy consumption caused by residue adhesion, improving wastewater treatment efficiency and equipment operational stability.

The utility model provides a kind of water quality microbiological detection culture device of precision temperature control, it is related to microbiological culture technical field, including temperature control box, the inside of temperature control box is provided with carrier box tray, the inside of carrier box tray is provided with six incubator, the outside of temperature control box is provided with oxygen regulation mechanism, oxygen regulation mechanism includes booster air pump, the output end of booster air pump is fixedly connected with gas pipe.The water quality microbiological detection culture device of precision temperature control, by the mutual cooperation of temperature control box, carrier box tray, incubator, sealing perspective door, sealing ring, oxygen sensor, connecting pipe, airtight solenoid valve, booster air pump, gas pipe, air pipe, gas pipe and button controller, each incubator can store appropriate water body sample with microorganism, and then when the batch of microorganism needs aerobic culture, operate button controller to control both airtight solenoid valves to be electrified and be communicated, and control booster air pump to be electrified and start pumping.

The application belongs to the technical field of microbial culture, and discloses a selective separation culture medium for gram-negative bacteria and application of the culture medium in rapid separation, purification and identification of gram-negative bacteria in samples such as aquatic products, food, water and soil. The selective separation culture medium takes a perylene quinone photosensitive compound as a key active component.

This invention belongs to the field of microbial culture technology. It provides a method for culturing cariogenic acid-producing anaerobic bacteria and its application. The culture medium comprises the following components in the following proportions: peptone 0.5-5%, sucrose 1-10%, sodium chloride 0.1-0.6%, bromocresol green 0.1-0.3%, bromocresol purple 0.01-0.15%, vitamin C 0.01-0.05%, Tween-80 0.01-0.06%, phenylethanol 0.01-0.25%, and deionized water, forming a liquid culture medium. Based on the specific requirements of bacterial culture, this invention is used for culturing bacteria from teeth and the oral cavity. Peptone is used as the nitrogen source, and sucrose as the carbon source. When bacteria multiply rapidly, they convert sucrose into an acidic substance, changing the pH of the culture medium. In the presence of bromocresol green / bromocresol purple, the color of the culture medium changes, allowing for the determination of bacterial content in the sample and the prediction of the risk of dental caries.

The present application relates to the technical fields of microbial culture, and particularly relates to an anaerobic bacteria culture device capable of regulating anaerobic degree and a use method thereof, comprising a vacuum pump, a CO2 gas cylinder, an H2 gas cylinder, an N2 gas cylinder and an anaerobic bottle, the vacuum pump is connected with the anaerobic bottle, each gas cylinder is connected with a proportioner to control the gas proportion in the anaerobic bottle, a culture tube is arranged in the anaerobic bottle, an opening is formed in the anaerobic bottle, a pressing plate for pressing a sealing film is hinged to the anaerobic bottle, and a driving assembly for driving the culture tube to move upward is arranged on the anaerobic bottle. The present application has simple structure, convenient operation, controllable culture environment and low price, and is suitable for small-scale enterprises and laboratory students. The strict anaerobic requirement in the experiment process is achieved through vacuum negative pressure adsorption. The gas proportion is regulated through the proportioner to meet different experimental requirements. The anaerobic degree of the environment can be further determined through the anaerobic indicator arranged in the system.

The application belongs to the technical field of microbial culture, and particularly relates to a bacillus cereus B5 and application thereof. The bacillus cereus B5 is isolated from an alpine environment of Qinghai-Tibet Plateau, and the preservation number is GDMCC No: 65417. The strain and secreted metabolites thereof can improve the mycelium formation rate of biocontrol fungi and insect resource fungi, and accelerate the formation process of conidia. Indoor detection is conducted on the influence of B5 on the growth of insect pathogenic fungi and the efficiency of killing host insects, and the results show that B5 can improve the mycelium formation rate of the tested strains, and simultaneously accelerate the growth rate of conidia, thereby providing technical support for fungal insecticides and artificial cultivation of fungal resource industry.

This invention belongs to the field of microbial culture technology, specifically relating to a culture medium formulation and corresponding culture method for efficiently inducing Cannomonas to produce perithecia. This invention provides a method for inducing Cannomonas (… Monosporascus cannonballus The culture medium for producing ascocarps: Add water to a mixture of carbon source, nitrogen source, inorganic salts, and plant tissue to a final volume of 1 L, adjust the pH to 7±0.1, add 20.0 g of agar powder, and then sterilize. This invention also provides a method for inducing ascocarp production in *Cannomonas*: Inoculate *Cannomonas* onto a culture medium plate, place the inoculated plate at 32±0.5℃, and culture using a light-dark cycle of 16 hours of light and 8 hours of darkness per day for 14-18 days. The method of this invention has technical advantages such as high sporulation efficiency and a short cycle.