135 results about "Microbial culture technique" patented technology

The invention relates to the technical field of microbial culture, and discloses a cell culture device, which comprises: a plate body; the culture units are arranged on the plate body at intervals in the width direction of the plate body, and each culture unit comprises a plurality of base holes which are arranged at intervals in the length direction of the plate body and are used for culturing cells and fluid channels which are arranged between every two adjacent base holes; according to the flowing direction of the culture medium along the fluid channels, the two sections of fluid channels corresponding to the two upward sides of each base hole are called the upstream fluid channel and the downstream fluid channel of the base hole; a downstream hole wall is arranged at least between the fluid channel at the downstream of the base hole and the base hole, and a plurality of downstream gaps allowing the culture medium to pass through and limiting the cells to pass through are formed in the downstream hole wall in a cutting manner.

The invention relates to the technical field of microorganism culture, and discloses a microorganism culture tank device which comprises a culture tank, a concave observation area is arranged in the center of the top of the culture tank, a central culture area surrounding the observation area is arranged in the culture tank, an oil path temperature control area wraps the culture tank, and a double-helix heat conduction oil path is integrated in the oil path temperature control area. Through the cooperation of the uniform distributor and the distributed simulated liquid conveying unit, the gas-liquid proportion and fluid distribution can be accurately regulated and controlled, the physical and chemical conditions in the deep sea or extreme environment are simulated, the constant-temperature precision of + / -0.005 DEG C is realized through the double-helix heat conduction oil way, the low-temperature environment is stably maintained, and the real thermodynamic conditions of deep-sea sediments are simulated; the device meets the sensitive requirements of different microorganisms on temperature, and has the characteristics of high practicability and capability of simulating a dynamic fluid environment of deep sea.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention provides a method for promoting generation of tea acid from Nemania sp YAFEF112, and relates to the technical field of microbial culture. According to the method, mycelia of Nemania sp YAFEF112 are inoculated into a culture medium containing pruned walnut branches for culture, the culture medium is an MM culture medium, and the specific formula of the culture medium comprises 1.52 g / L of monopotassium phosphate, 0.52 g / L of magnesium sulfate, 6 g / L of sodium nitrate and 0.52 g / L of potassium chloride. According to the method, the defects in the prior art are overcome, and good conditions are provided for the production of the tea pickled acid by optimizing the formula of the fermentation culture medium, adding pruned walnut branches into the culture medium, improving the fermentation conditions and increasing the content of the tea pickled acid in the Nemania sp YAFEF112 hyphae.

The invention discloses a microbial culture device for microbial inspection experiments, and relates to the technical field of microbial culture. The device comprises an incubator, the incubator is slidably connected with a sleeve, the sleeve is internally slidably connected with a sliding rod, the sliding rod is rotatably provided with a clamping mechanism, the clamping mechanism comprises a first disc arranged on the sliding rod, the first disc is rotatably installed on the sliding rod, the first disc is provided with an arc-shaped sliding groove, and the arc-shaped sliding groove is slidably connected with a protruding block; the protruding block is fixedly installed on a clamping plate, the clamping plate is rotationally installed on a second disc, and annular racks are fixedly installed on the first disc and the second disc correspondingly. Through the clamping mechanism and the sleeve and the sliding rod which are in sliding connection, the culture tube can be independently taken out or put in under the condition that the whole culture box is not opened, the design avoids fluctuation of the temperature and humidity in the culture box due to frequent opening and closing of the box door, and therefore it is ensured that microorganisms grow in a stable and appropriate environment.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention relates to the technical field of endoscopic microbial culture, and discloses a dynamic perfusion control system and method for an endoscopic microbial culture agent, and the system comprises a multi-channel data collection module which is used for obtaining multi-dimensional culture agent parameters including concentration distribution, perfusion rate and metabolite in real time, wherein the concentration distribution data comprises first environment distribution data generated by the multi-region perfusion device and second dynamic feedback data collected by the optical sensor array; the dynamic regulation and control module performs gradient equilibrium correction processing on the parameters and inputs the parameters into the perfusion analysis processing layer for feature modeling, the perfusion analysis processing layer comprises a data preprocessing module and an environment modeling module, and the environment modeling module comprises an environment parameter fusion layer, a gradient equilibrium layer and a perfusion decision-making layer which are connected in sequence. And finally, generating a perfusion regulation and control instruction according to an output result. According to the invention, real-time monitoring and dynamic accurate regulation and control of multi-dimensional parameters are realized, and the quality and efficiency of endoscopic microorganism culture are improved.

The invention belongs to the technical field of microbial culture, and particularly relates to a culture medium composition and application thereof in PDRN recombinant escherichia coli fermentation. The culture medium composition comprises a fermentation culture medium and a fed-batch culture medium, the fermentation culture medium consists of disodium hydrogen phosphate, monopotassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate tetrahydrate, glycerol and water; the supplemented culture medium is composed of dipotassium phosphate, sodium dihydrogen phosphate, sodium sulfate, ammonium sulfate, ammonium chloride, diammonium hydrogen citrate, glycerin and water, and the metabolic requirements of PDRN recombinant escherichia coli are accurately matched. Meanwhile, the invention provides a fermentation method using the composition, and the problems of unbalanced nutrition, low fermentation efficiency and the like of an existing culture medium are solved. Stable proliferation of thalli can be remarkably promoted, the yield of PDRN products is increased, the culture medium is easy and convenient to prepare, fermentation conditions are easy to control, and the culture medium is suitable for industrial large-scale production and has important application value.

The invention discloses a microorganism culture and storage device and a culture and storage method, and belongs to the technical field of microorganism culture. The microorganism culture and storage device comprises a box body, a clamping mechanism for clamping culture dishes is arranged in the box body, the clamping mechanism comprises a mounting plate, a plurality of clamping grooves for placing the culture dishes are formed in the mounting plate, and clamping structures are arranged on the side walls of the clamping grooves. A transmission structure for driving the clamping structure to act is arranged between the fixing plate and the mounting plate in the incubator; a stirring structure for stirring a culture solution and ventilating is arranged in the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged in the box body. By adopting the microorganism culture and storage device and the culture and storage method, the problems that metabolic substances are difficult to detect and the culture dish is unstable to fix in the microorganism culture process by adopting the culture dish can be solved.

The invention relates to the technical field of microorganism culture, in particular to a planting culture medium microorganism fermentation culture tank which comprises a tank body, a motor fixedly installed at the top end of a tank cover on the upper portion of the tank body, a stirring shaft fixedly connected to the bottom end of a motor driving shaft and a stirring paddle fixedly installed on the outer side of the middle of the stirring shaft. The device is characterized in that a defoaming assembly is arranged between the outer side of the top of the stirring shaft and the inner wall of the tank body, a scraping ring assembly is arranged at the top of the defoaming assembly, and a homogenizing assembly is arranged between the bottom end of the defoaming assembly and the bottom of the stirring shaft. The rotating stirring shaft drives the defoaming paddle and the collision block to rotate, so that the collision plate collides with the collision block, then the scraping ring is driven to rotate and slide to scrape fermentation liquor on the inner wall, and meanwhile, the stirring teeth at the bottom can perform horizontal rotary stirring and linear reciprocating stirring perpendicular to the stirring shaft; and the mixing density of bottom fermentation liquor is more uniform.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

The embodiment of the utility model discloses a microorganism expanding culture device which comprises a microorganism culture bin, a filler unit and an aeration unit, the side wall of the microorganism culture bin is provided with a feed port, a dosing port and a discharge port, and the feed port and the dosing port are respectively used for inputting reaction raw water and nutrient substances into the microorganism culture bin; the discharge port is used for discharging reacted water in the microorganism culture bin to the outside; the filler unit is arranged in the microorganism culture bin, and the filler unit is used for attachment growth of microorganisms; the aeration unit is arranged in the microorganism culture bin and is positioned at the bottom of the filler unit; the aeration unit is used for providing oxygen for microorganism breeding in the microorganism culture bin. The microorganism expanding culture device adopts a mud-film symbiotic method microorganism culture technology to promote microorganisms to be in an efficient propagation state all the time, so that continuous and efficient microorganism amplification is realized.

The invention discloses a microbial culture device, and belongs to the technical field of microbial culture, the microbial culture device comprises a support frame composed of a plurality of connecting rods, the upper side of the support frame is fixedly connected with a placement rack, the top of the support frame is rotatably connected with two symmetrical screw rods, and the two screw rods are connected through a transmission part; a driving part is mounted at one end of one lead screw, and the lead screws penetrate through the placement frame; an incubator is connected to one side, close to the driving part, of the screw rod through threads, and the incubator is located on the placement frame; a temperature control part is installed in the middle of the bottom wall of the placing frame, the incubator is of an inverted-L-shaped structure, and the bottom wall of the incubator is attached to the temperature control part; a circulation assembly is installed on the side, away from the containing frame, of the bottom of the supporting frame. The device has the advantages of local temperature supply, rapid switching to room temperature and automatic discharge of the culture solution.

The invention discloses a silicon rubber surface green algae adhesion layer cultivation method and a cultivation device, the method comprises algae concentration, surface pretreatment, spraying treatment, green algae inoculation and cultivation, the cultivation method specifically comprises the following steps: placing an inoculated silicon rubber sample in the cultivation device, controlling the temperature to be 25-30 DEG C, the humidity to be 70-90%, the illumination period to be 12 h / 12 h, the illumination intensity to be 100-200 mol.m <-2 >. S <-1 >, and the illumination time to be 12 h / 12 h; and continuously supplying the culture solution through the hanging type dropper to maintain the growth of the green algae, and continuously supplying the culture solution through the hanging type dropper to maintain the growth of the green algae. The method can simulate light, temperature and gas conditions in a natural environment, and realizes period-controllable and density-controllable green alga adhesion layer construction in a laboratory environment by combining a microbial culture technology, so that the rule of influence of green alga adhesion on the performance of the composite insulator is more accurately mastered; scientific basis and technical support are provided for prevention and treatment of external insulation biological contamination of an electric power system.

The invention provides a microorganism composite culture device and method, and belongs to the technical field of microorganism culture.The microorganism composite culture device comprises a frame, an algae pool, a microorganism culture pool and an algae-eating insect culture pool are sequentially arranged in the frame from inside to outside, a center cylinder is arranged in the middle of the algae pool, and a mounting partition plate is arranged in the lower end of the center cylinder; a water conveying device is arranged in the middle of the installation partition plate, the water pumping end of the water conveying device is located on the lower portion, the water drainage end of the water conveying device is located in the algae pond on the upper portion, the algae pond and the microorganism culture pond are separated through a first partition plate, a sunlight shed is arranged at the upper end of the first partition plate, and the microorganism culture pond and the algae-eating insect culture pond are separated through a second partition plate. A carrier frame is arranged in the microorganism culture pond; the algae pool, the microorganism culture pool and the algae-eating insect culture pool are distributed in the frame body, so that algae, bacterial microorganisms, corophium and algae-eating insects can be subjected to co-culture, and a compound microorganism culture system is constructed.

The utility model relates to the technical field of saline-alkali soil microorganism culture, in particular to a storage device for saline-alkali soil microorganism culture, which comprises a storage box, a plurality of storage grooves are embedded in the top of the storage box, a plurality of culture cups are placed in the storage grooves, and a storage cavity and a lifting groove are respectively arranged on two sides of each culture cup. A sealing sliding plate is arranged in the storage cavity in a sealing and sliding fit mode, a sliding column which is in vertical sliding fit with the culture cup and can penetrate through the lifting groove is connected to the sealing sliding plate, the outer wall of the culture cup is provided with an inner concave surface and an annular groove which are communicated with each other, and a positioning opening is formed in the annular groove; according to the saline-alkali soil microorganism extraction device, microorganisms can be conveniently extracted under the condition that the soil structure of saline-alkali soil is not damaged, and meanwhile the saline-alkali soil microorganism extraction device is convenient and fast to use.

The invention discloses a device and a method for breeding microorganisms in distiller's yeast, belongs to the technical field of microorganism culture, and aims to solve the problem that when an existing microorganism culture device promotes uniform mixing of microorganisms, a nutrient solution and oxygen through stirring, the nutrient solution on the inner wall of the device is poor in fluidity, and the contact oxygen dissolving efficiency is affected. The reciprocating assembly drives the moving rod to push the shifting disc to move up and down in the cavity of the adjusting barrel and the space above the shifting disc, after the shifting disc moves out of the top port of the adjusting barrel, a nutrient solution carrying microorganisms is poured into the adjusting barrel, the shifting disc moves downwards to press the nutrient solution in the adjusting barrel, and the nutrient solution is squeezed and pushed to be sprayed out of the liquid spraying pipe at the bottom of the adjusting barrel. The sprayed fluid drives the raised microorganisms and nutrient solution to uprush; the nutrient solution in the cultivation cavity is circularly injected into the adjusting cylinder and then is extruded and sprayed out, so that the circulating flow rate of the nutrient solution adjacent to the bottom and the inner wall of the cultivation cavity is accelerated, the nutrient solution is fully contacted and fused with oxygen sprayed out of the breather pipe, and the influence on proliferation and growth of microorganisms due to insufficient oxygen supply of the microorganisms in the nutrient solution is avoided.

The present invention discloses a microbial culture storage device and a culture storage method, belonging to the technical field of microbial culture. The microbial culture storage device includes a box body, and a clamping mechanism for clamping culture dishes is arranged inside the box body. The clamping mechanism includes a mounting plate, a plurality of card slots for placing culture dishes are arranged on the mounting plate, a clamping structure is arranged on the side wall of the card slot, and a transmission structure for driving the clamping structure to act is arranged between the fixing plate in the incubator and the mounting plate; a stirring structure for stirring the culture solution and performing ventilation is arranged inside the shell of the culture dish, and a power structure for driving the stirring structure to rotate is arranged inside the box body. By using the microbial culture storage device and the culture storage method of the present invention, the problems that it is not easy to detect metabolic substances during the process of microbial culture using culture dishes and the culture dishes are not stably fixed can be solved.

The invention relates to the technical field of microorganism culture, in particular to a ganoderma sinensis spore germination culture medium and application thereof.Ganoderma sinensis spores are collected through a fungus carrying bag or the culture medium, the optimal growth environment for ganoderma sinensis spore germination culture is obtained by optimizing the culture condition of the ganoderma sinensis spores, and the ganoderma sinensis spore germination culture medium is obtained by optimizing the culture medium formula and adding wood chip boiling liquid. A culture medium formula most suitable for ganoderma spore germination is obtained through raw material screening and orthogonal experiment optimization of boiling liquid, ganoderma spores can be effectively collected without reaching a sterile environment of a laboratory, the method is a practical and effective ganoderma sinensis spore collection and germination method, and a foundation is laid for promoting ganoderma sinensis new variety breeding research.

The utility model discloses an experimental device for ultraviolet mutagenesis of fungi, which belongs to the technical field of microbial culture and comprises an experimental cabinet main body, a movable cabinet door is rotatably connected to the outer end of the experimental cabinet main body, four uniformly distributed ultraviolet irradiation lamps are mounted at the top end in the experimental cabinet main body, and two lifting strips are fixedly connected to the left inner wall and the right inner wall of the experimental cabinet main body respectively. A through hole is formed in the outer end of the lifting block, a positioning block is slidably connected into the through hole, a connecting plate is fixedly connected into the lifting block and located on the outer side of the positioning block, a threaded rod is connected into the connecting plate in a threaded mode, and the threaded rod is located at the outer end of the positioning block and rotationally connected with the positioning block. A plurality of positioning grooves which are uniformly distributed and matched with the positioning blocks are formed in the outer end of the lifting strip, so that the experiment space layout can be flexibly adjusted by adjusting the positions of the lifting blocks on the lifting strip, and the space utilization rate is improved.

The invention discloses a microbial culture device convenient to increase dissolved oxygen, and relates to the technical field of microbial culture.The microbial culture device comprises a culture tank and a mounting shaft, a rotating pipe is fixedly arranged at the upper end of the mounting shaft, mixing frames are symmetrically arranged on the rotating pipe, a plurality of bubble nozzles are arranged at one ends of the mixing frames, and an oxygen supply assembly is arranged at the upper end of a sealing cover; a mounting pipe is rotatably arranged at one end of the mounting shaft, a conveying cylinder is fixedly arranged at one end of the mounting pipe, an auger plate is arranged on the mounting shaft, a driving assembly is arranged at the bottom end of the mounting cylinder, a protection mechanism is arranged on the mounting shaft, and a cleaning mechanism is arranged at the upper end of the culture tank. A plurality of bubble nozzles are arranged at one end of the mounting shaft, so that the bubble nozzles can generate microbubbles conveniently, dissolved oxygen of microorganisms can be increased uniformly, meanwhile, the microorganisms can flow circularly through cooperative use of a transmission cylinder and an auger plate, and floating foam can be cleaned conveniently by arranging a shifting rod at the upper end of a liquid guide ring.

The application discloses a culture dish filling device and a use method, relates to the technical field of microbial culture, and solves the problems of complex operation, high use cost and high pollution risk of the existing culture medium filling mode, reduces the operation difficulty and the pollution risk, and specifically has the following arrangement: the device comprises a filling module and a filling bag hung on the filling module, the filling module is composed of a driving unit and a supporting block connected with the driving unit through a telescopic rod, the filling bag is hung on the supporting block, a rotating part is arranged on the supporting block, the rotating part is driven by a rotating motor arranged in the supporting block, a rotating switch is arranged on a filling head at the bottom of the filling bag, and the rotating switch is hung on the rotating part.

The invention provides a probiotic culture medium and a method for preparing a probiotic preparation, and belongs to the technical field of microbial culture. According to the preparation method, the probiotic culture medium is firstly prepared, the probiotic compound is obtained by utilizing the probiotic culture medium and bifidobacteria, and then the dietary fiber compound, the quercetin compound and the liquorice-rhizoma cyperi compound are used together, so that the probiotic preparation is obtained, and the preparation can effectively improve related symptoms of intestinal tracts, stomachs and respiratory tracts.

The present invention discloses a method for rapidly enriching and isolating purple non-sulfur bacteria from sewage treatment systems. This method optimizes the PNSB enrichment medium and uses infrared anaerobic methods to rapidly enrich high concentrations of PNSB from complex sewage environments. Simultaneously, a single PNSB strain is isolated using the optimized isolation medium and a simple light anaerobic device. The method provided by the present invention enables rapid enrichment and isolation of PNSB from complex environments, resulting in higher bacterial purity and simpler isolation methods, thus possessing important practical applications in microbial culture technology.

The culture medium comprises an incubator, the top of the incubator is provided with a detection unit for detecting the number of microorganisms in the incubator, and the top of the incubator is further provided with a quantitative feeding mechanism for quantitatively feeding a culture solution; the invention relates to the technical field of microbial culture. According to the mass microorganism culture medium, through the arrangement of the quantitative feeding mechanism and the driving assembly, automatic quantitative feeding of a culture solution is achieved, the culture solution is quantitatively fed and discharged once by pressing a pressing cylinder once, meanwhile, a rotating plate rotates by 90 degrees, and the quantitatively fed culture solution falls into a culture box through a fan-shaped groove; along with culture and reproduction of microorganisms, quantitative feeding is carried out multiple times, the amount of the culture solution is increased, the concentration and the amount of the culture solution are synchronously increased when the microorganisms are gradually increased, quantitative feeding facilitates statistics of feeding times, and therefore the concentration and the amount of the culture solution are calculated, and precise culture of the microorganisms is achieved.

The invention relates to the technical field of microbial culture, in particular to an efficient tremella aurantialba liquid strain culture medium and a preparation and culture method. The feed comprises the following components in percentage by mass: 0.5% of corn flour; 3% of glucose; 0.4% of peptone; 0.2% of monopotassium phosphate; 0.1% of magnesium sulfate; 0.001% of vitamin B; and the balance of water. The method has the beneficial effects that 1, the growth efficiency is obviously improved; 2, the strain quality is stable and controllable; 3, the advantage of production cost is outstanding; operation is easy and convenient, and popularization is easy.

The invention relates to the technical field of microbial culture, in particular to a method for increasing the titer of Klebsiella pneumoniae bacteriophage, which comprises the following steps: step 1, culturing host bacteria; step 2, regulation and control of infection complex number: setting a plurality of infection complex numbers of different bacteriophage-klebsiella pneumoniae number ratios, and standing to obtain a mixed solution; and step 3, lysis culture: carrying out shake culture on the adsorbed mixed solution for 1.5-2 hours until the solution becomes clear, observing the clarity change of the solution, filtering to obtain a bacteriophage suspension, and determining the preferable infection complex number and bacteriophage titer by measuring the bacteriophage suspension titer. According to the method, by optimizing host bacterium culture conditions and infection complex number regulation and control and combining a standardized splitting decomposition judgment method of light transmittance and visual colorimetry, under the condition that centrifugal concentration or advanced equipment is not needed, the titer of the klebsiella pneumoniae bacteriophage is remarkably improved, and efficient, low-cost, broad-spectrum and safe bacteriophage preparation is achieved.

The present invention relates to the technical field of microbial culture, and discloses a cell culture device capable of synchronously replacing culture medium, comprising: a box body, comprising a plate body and a surrounding plate, wherein the surrounding plate and the upper surface of the plate body form a main pool capable of accommodating culture medium; a base hole, which is opened on the upper plate surface of the plate body, and the base holes include a plurality of base holes, which are arranged in a matrix; a filter cartridge, wherein a filter cartridge is provided above each base hole, and a downstream flow gap is provided on the cartridge wall of the filter cartridge on at least the downstream side; a sinking cavity is provided on the upper plate surface of the plate body on the upstream side of each base hole, and an upstream hole wall is defined between the sinking cavity and the base hole, and a first upstream flow gap is provided on the upstream hole wall; a water dam is provided between each two adjacent filter cartridges, and adjacent rows of water dams and filter cartridges separate the main pool into pool units, and each row of water dams enables the culture medium in the pool unit to actively flow to the sinking cavity and enter the base hole through the first upstream flow gap.

The invention discloses a culture method for improving freeze-drying stress resistance and storage stress resistance of bifidobacterium and application of the culture method, and belongs to the technical field of microorganism culture. The method comprises the following steps: firstly, establishing a freeze-drying model of the pH dynamic regulation thalli for evaluating the influence of freeze-drying and storage activity of the thalli; based on the established freeze drying model of the pH dynamic regulation thalli, better culture conditions before freeze drying and better temperature during storage of the bifidobacterium are screened. According to the culture method for improving freeze-drying stress resistance and storage stress resistance of bifidobacteria and the application of the culture method, by regulating and controlling the active state of cultured thalli, the later-stage drying and storage stress resistance of strains is improved, and a foundation is provided for precise culture of probiotics and processing of active preparations.