144 results about "Microbial culture technique" patented technology

The invention provides a method and a device for collecting microalgae through continuous culture and in-situ self-flocculation and belongs to the technical field of microbial culture. The method comprises the following steps of: culturing microalgae cells through a bubble stirring column type photobioreactor, separating the microalgae cells through an in-situ sedimentation method, and discharging concentrated microalgae slurry from the bottom of a cone at the lower end of the column; and directly supplementing in flocculated supernatant and continuously culturing the microalgae cells. The organic wastewater recycling, CO2 biological fixation and continuous microalgae culture and separation can be simultaneously realized, the cost of culturing microalgae in a large scale is reduced, and the culture and recovery efficiency is improved; the culture solution is recycled, so that the treatment quantity is greatly reduced; the microalgae culture and recovery process is simple and is convenient to operate; and moreover, the equipment is simple in structure, low in cost and wide in application range and has the potential of industrial popularization and application.

The invention relates to marine microorganism cultivation technology, specifically to a medium used for culturing Schizochytrium limacinum and a production method for DHA by using the medium. The medium provided by the invention is a medium with a low chlorine ion concentration and has a chlorine ion concentration of 2 g/L, a potassium ion concentration of higher than 0.1 g/L and a sodium ion concentration of 1 to 20 g/L. With the medium provided by the invention, the output of DHA can basically maintain same or be higher, the degrees of corrosion of the medium on a series of production equipment like fermentation equipment, oil pressing equipment and extraction equipment are reduced, the service life of the equipment is prolonged, production safety is guaranteed, and the chlorine ion concentration in discharged sewage during production is reduced, thereby further reducing production cost.

The invention relates to the technical field of environmental simulation and microorganism culture, and in particular relates to a deep sea thermal solution simulation and high-temperature high-pressure microorganism culture system comprising a gas and liquid transmission pipeline, and further comprising a high-pressure substrate batching unit for uniformly mixing high-pressure gas and high-pressure seawater to obtain a high-pressure seawater culture medium dissolved with high-concentration gas, a high-temperature high-pressure culture unit for acquiring the high-pressure seawater culture medium from the high-pressure substrate batching unit to breed microorganism, a gas supply unit for providing high-pressure gas for the high-pressure substrate batching unit and the high-temperature high-pressure culture unit, a flow control unit for controlling pressure in the high-pressure substrate batching unit and the high-temperature high-pressure culture unit as well as a flow velocity in a continuous culture process, and a gas monitoring control unit for detecting concentration of combustible gas in an environment, judging leakage of the combustible gas in the high-pressure substrate batching unit and the high-temperature high-pressure culture unit, and alarming in real time or automatically cutting off gas supply of the gas supply unit depending on a judgment result.

The invention discloses an in-situ culturing device for deep-sea microbes. The device comprises sample culturing boxes, culturing box placement cases and a placement case protection cover, wherein each sample culturing box comprises a box body, an upper locking cover and a lower locking cover; a sealing gasket, a filter membrane, a sealing gasket, the box body, a sealing gasket, a filter membrane and a sealing gasket are sequentially arranged between each upper locking cover and the corresponding lower locking cover; more than one sample culturing box can be accommodated in each culturing box placement case; more than one culturing box placement case can be accommodated in the placement case protection cover. The device has the advantages that the device can be directly arranged in deep sea, the natural environment of the deep-sea microbes can be maximally restored, and the deep-sea microbes can naturally grow; chemical substances can freely penetrate through the filter membranes, so that interaction between microbial communities can be ensured, and the culturability of the microbes is improved; the shortcoming of incapability of the conventional microbe culturing technology in simulating or completely simulating the natural conditions of existence for marine microbes is overcome, and the bottleneck in researches on nonculturable microbes is broken.

The invention relates to a biochemical culture and detection device as well as a detection method of the device, belonging to the technical field of microorganism culture. The biochemical culture and detection device comprises a bottom plate (1) and a top cover (3), wherein the bottom plate (1) comprises a culture pond (11) and a receiving region (12); bumps (122) in the receiving region are radially arranged around the culture pond (11) and used for dividing the receiving region (12) into multiple fan-shaped storage locations; culture sub-grooves (2) are like hollow wedge-shaped bodies and are arranged in the storage locations in the receiving region (12); the culture sub-grooves (2) adopt fully-opened culture sub-grooves (2a) or partially-opened culture sub-grooves (2b); the culture pond (11) is used for receiving liquid culture mediums to be detected; and the culture sub-grooves (2) are used for receiving detected culture mediums. The incubating process is finished by rocking the biochemical culture and detection device. The functions of enriched culture, coated incubation and biochemical identification are integrated, the operation is flexible, the requirement on operating environment and the detection cost are lowered, and the detection efficiency and the stability of results are improved.

The invention discloses an enzyme solution with a better enzyme activity ratio of beta-mannase to alpha-galactosidase as well as a preparation method and application of the enzyme solution, and belongs to the technical field of microbial culture. According to the preparation method, trichoderma reesei is taken as an enzyme-producing strain, microcrystalline cellulose and/or melibiose is taken as a carbon source, the enzyme solution is produced by adopting a fermentation method, the enzyme activity of the beta-mannase in the enzyme solution is not higher than 0.05 U/mL, and the enzyme solution has a better enzyme activity ratio of beta-mannase to the alpha-galactosidase. The enzyme solution can directly hydrolyze the galactomannan to prepare micromolecular galactomannan and galactomannan-oligosaccharide without purification, the yield of the micromolecular galactomannan and the galactomannan-oligosaccharide can be effectively increased, the production cost is reduced, and the enzyme solution has a very good application prospect.

The invention relates to a microorganism culture detection device in an oxygen-controllable environment and a detection method using the same, belonging to the technical field of microorganism culture. The microorganism culture detection device comprises a culture bottle (1), a detection cover (2), a diaphragm (3) and a diaphragm fixing device (4), wherein the middle part of the detection cover (2) is provided with a hollow reaction cavity (21) protruded upwards; the center of the diaphragm fixing device (4) is provided with a through hole (41) and a groove (411); the front surface of the diaphragm fixing device (4) is provided with a concave die cavity (412) and a ventilation port; a detection reagent is placed in the reaction cavity (21) of the detection cover (2); an oxygen eliminating agent or oxygen generating agent is placed in the die cavity (412); a sample to be detected is inoculated with a liquid culture medium in the culture bottle (1); and the culture bottle (1) is in threaded connection with the detection cover (2) in a separated manner. The invention has the advantages that the structure is simple, the components are convenient to replace and the oxygen-controllable environment is easy to realize, and is suitable for industrial production, storage and transportation. Besides, the invention has the characteristics of flexible operation, quick and sensitive detection and high accuracy.

The invention provides a solid state fermentation medium for paecilomyces lilacinus and a method for culturing the paecilomyces lilacinus and belongs to the technical field of microbial culture. The solid state fermentation medium comprises the following components in parts by weight: 5-40 parts of wheat bran, 1-10 parts of rice husk, 1-10 parts of corncobs, 10-70 parts of mushroom residues and 10-70 parts of fruit residues. The method comprises the following steps: 1) inoculating activated paecilomyces lilacinus into a primary liquid medium for performing primary culture so as to obtain a primary seed solution; 2) inoculating the primary seed solution into a secondary liquid medium for performing secondary culture; and 3) inoculating the secondary seed solution into the solid state fermentation medium for performing solid fermentation culture for 5-8 days. When the solid state fermentation medium provided by the invention is utilized for culturing the paecilomyces lilacinus, the fermentation time can be shortened, the fermentation cost is reduced, and the yield of the paecilomyces lilacinus is increased.

The invention relates to a sulfate reducing bacteria microbe corrosion method of magnesium alloy, for resolving the microbe corrosion problem of magnesium alloy and combining the microbe cultivation technique and microbe corrosion technique of solid culture medium to provide a novel magnesium alloy microbe corrosion method based on solid culture medium. The method comprises (1) processing and disinfecting sample, (2) arranging and disinfecting a solid culture medium, (3) cultivating and activating bacterial, (4) corroding paster. The invention optimizes the components of electrolyte to highlight the bacterial performance in the corrosion process of magnesium alloy, and compares the electrode surface corrosion morphology with bacterial and without bacterial, to provide the reference for magnesium alloy microbe corrosion mechanism research. The inventive method is suitable for magnesium alloy microbe corrosion research.

The invention relates to a multi-phase co-culture and detection method and a device thereof, and belongs to the technical field of microbe culture. According to the multi-phase co-culture and detection device, a culture tank (1.1) and a culture chamber (1.2) with a plurality of small chambers are formed on a base plate; and liquid culture media are injected into the culture tank (1.1), and various solid or semisolid culture media are injected into the small chambers of the culture chamber (1.2). The liquid culture media are coated to the solid or semisolid culture media by shaking the device to complete the inoculated culture process of bacterial colonies and obtain corresponding detection results. By the device, the solid-phase culture media, the liquid-phase culture media and the semisolid-phase culture media can be separated with each other and can contact with each other under a certain physical condition, so that the complex operation of inoculating bacteria between different culture media is avoided, a false positive result caused by microbial pollution of environment is reduced, the culture and detection of microbial samples are simplified, the stability of detection efficiency and detection results is improved, and the culture and detection can also be performed on a large scale.

The invention discloses an isolated culture method for nilaparvata lugens in-vivo yeast-like symbiotic bacteria and a special culture medium, and belongs to the technical field of microculture. The special culture medium comprises 31 nutrient substances needed by nilaparvata lugens in-vivo yeast-like symbiotic bacteria, and the culture method comprises the following steps of: feeding adult nilaparvata lugen; treating ovaries of nilaparvata lugens; preparing culture solution, performing isolated culture and the like. By employing the culture method, two yeast-like strains are isolated from the nilaparvata lugens in vivo so as to provide basis for further researching genetic background of the yeast-like symbiotic bacteria and the interavailable nutrient relation between the yeast-like symbiotic bacteria and host nilaparvata lugens, and modifying the symbiotic bacteria to control the harm of the nilaparvata lugens. Meanwhile, the special culture medium is reasonable in components and provides necessary nutrition basis for isolated subculture of the nilaparvata lugens in-vivo yeast-like symbiotic bacteria. The method also provides technical support for isolated culture of other homoptera insect in-vivo yeast-like symbiotic bacteria.

The invention belongs to the technical field of microbe culture and relates to a culture method of a cordyceps sinensis anamorphic hirsutella sinensis pure strain. The culture method comprises the following steps: A sampling: acquiring a wild cordyceps sinensis stroma of which ascospore is not ejected out, and storing; B separating: taking a strain in the cordyceps sinensis stroma, and inoculating into a flat plate under a sterile condition; C culturing, performing constant-temperature culture of the flat plate containing the strain in a culture box to obtain a hirsutella sinensis bacterial colony; D purifying, transferring the bacterial colony in the step C to a new flat plate for culture to obtain the hirsutella sinensis pure strain. By adoption of the culture method, the hirsutella sinensis pure strain can be obtained more easily, and the culture method is suitable for separating the cordyceps sinensis strain.

The invention belongs to the technical field of microbial culture, and specifically relates to a rhodopseudomonas palustris culture medium and an application thereof. The rhodopseudomonas palustris culture medium has a formula composed of citric acid, a beef extract, a yeast extract, peptone, sodium acetate, magnesium sulfate, ferrous sulfate, VB6, calcium chloride, monopotassium phosphate and a composite inorganic salt solution, wherein a solvent is water. According to the invention, through optimization of the formula, the quality of a rhodopseudomonas palustris inoculum is greatly improved,so the rhodopseudomonas palustris inoculum is high in effective viable count, pure in color, free of layering, low in infectious microbe rate, stable in quality and good in preservation effect, and the rhodopseudomonas palustris inoculum cultured by using the culture medium provided by the invention has good effect when being applied to aquaculture.

The invention provides a hirsutella sinensis expansion culture medium and hirsutella sinensis cultured by same as well as a culture method, belonging to the technical field of microorganism culture. Every 1L of the expansion culture medium is prepared from the following components: 8-12g of silkworm chrysalis extract, 15-25g of glucose, 8-12g of peptone, 3-8g of yeast powder and 0.05-0.2g of vitamin B1, and water is used for fixing the volume to 1L. Compared with the natural cordyceps sinensis, the hirsutella sinensis cultured by using the expansion culture medium provided by the invention islittle in differences, so that the hirsutella sinensis has the potential to become a substitute for the cordyceps sinensis.

The invention belongs to the technical field of microbiological culture, and particularly relates to a culture method of staphylococcus nepalensis capable of tolerating a high-salinity environment. The staphylococcus nepalensis capable of tolerating the high-salinity environment is characterized in that the staphylococcus nepalensis is preserved in China Center for Type Culture Collection on September 16, 2020, and the preservation number is CCTCC NO: M 2020505. The culture method of the staphylococcus nepalensis comprises the following steps: S1, preparing a staphylococcus nepalensis culturemedium; S2, preparing a staphylococcus nepalensis fermentation culture medium; and S3, performing fermentation culture. The staphylococcus nepalensis has the beneficial effects that the staphylococcusnepalensis is excellent in high-salinity tolerance and can adapt to treatment of wastewater in the high-salinity environment, and a novel microbiological treatment method is provided for treatment ofhigh-salinity fermentation wastewater; The strain is excellent in high-salinity wastewater treatment effect; and the content of residual sugar in the fermentation wastewater can be reduced to 0.3%-0.8%.

The invention belongs to the technical field of microbial culture, particularly relates to a culture method of Staphylococcus sciuri capable of resisting a high-salt environment, and further relates to application of the Staphylococcus sciuri in treatment of fermentation production wastewater. The application of the Staphylococcus sciuri F-E8-1 capable of resisting the high-salt environment in rapid degradation of residual sugar in the fermentation production wastewater of organic acid and/ or amino acid in the high-salt environment is the range of key protection of the invention. The specificfermentation production wastewater is treated through a microbial strain, so that the Staphylococcus sciuri F-E8-1 has the significant advantages of being low in operation cost, low in consumption, high in efficiency, high in adaptability, relatively stable in operation, convenient and fast to operate and manage and the like. The Staphylococcus sciuri provided by the invention has excellent high-salt resistance, and is good in treatment effect in the treatment process of a wastewater body with high salt content. The high-salt wastewater is treated by adopting the strain disclosed by the invention, so that the consumed time is short and the treatment effect is good.

The invention belongs to the field of microbial culture technology, relates to a process technology adopting a bacillus mucilaginosus industrial liquid to form spores, and particularly relates to a submerged fermentation culture medium of bacillus mucilaginosus applied to microbial fertilizers. The culture medium comprises the following raw materials according to the weight percentage: 0.9-1.5% of corn flour, 0.1-0.3% of soybean meal, 0.08-0.2% of dipotassium phosphate, and the balance being water. The culture medium is obtained by the steps of mixing the corn flour, the soybean meal, dipotassium phosphate and water, stirring evenly, sterilizing for 20-40 min at the temperature of 121 DEG C, and then cooling to 25-40 DEG C to obtain the culture medium. The pH value of the culture medium is in the range of about 6.0-7.5, and the spores of bacillus mucilaginosus grow well in the pH value of 6.0-8.0, so the culture medium can promote thalli growth and spore formation without using an acid or alkali to adjust pH before sterilization, simplifies operation, improves the product quality, reduces the production cost, and is more suitable for industrial mass production.

The invention discloses a temperature-adjustable microorganism culture device and method, and relates to the technical field of microorganism culture. The device comprises a culture box, a base and aculture dish, wherein the bottom of an inner cavity of the culture box is fixedly connected with the bottom of the base, the top of the base makes contact with the bottom of the culture dish, a driving box penetrates through the top of the culture box, the top of an inner cavity of the driving box is fixedly connected with a motor through a supporting plate, and the output end of the motor is fixedly connected with a threaded rod through a coupler. According to the temperature-adjustable microorganism culture device and method, external air can be prevented from directly entering the culture box, so that bacteria in the air are unlikely to pollute a culture medium in the culture box; and moreover, manual operation is not needed when a culture solution or water needs to be added, the overall culture effect is good, the culture dish can be well fixed, and the temperature in the culture box can be controlled in real time.

The invention provides a method for culturing clostridium butyricum through mixed fermentation, and belongs to the technical field of microbial culture. The method includes the following step that single-bacterium culture solutions of clostridium butyricum, bacillus subtilis, yeast and lactic acid bacteria are mixed for mixed culture, wherein the temperature of mixed culture is 29 DEG C, the pH value of mixed culture is 6.4-6.8, the time for mixed culture is 16-20 hours, the rotating speed of mixed culture is 150-170 rpm, and the mixing ratio of clostridium butyricum to yeast to bacillus subtilis to lactic acid bacteria is 1:1:3:1. The method is simple to operate, the biological activity of cultured clostridium butyricum is remarkably improved, and the biological activity is greatly improved.

The invention belongs to the technical field of microculture, and discloses a streptomycete fermentation medium. The streptomycete fermentation medium is prepared from, by weight, 7.3-9.2% of straw zymolyte, 4.2-6.8% of potato diffusion juice, 3.3-5.5% of bean pulp hydrolysate, 2.6-4.7% of fishbone meal hydrolysate, 2.3-3.7% of wheat bran, 1.5-2.6% of glucose, 0.1-0.2% of dipotassium phosphate, 0.05-0.08% of magnesium sulfate, 0.02-0.03% of sodium chloride and the balance water. The streptomycete fermentation medium is low in cost and increases enterprise profits.

The invention provides a microbionation device and belongs to the technical field of microculture. The microbionation device comprises an interface and a soft inoculation rod, the top end of the soft inoculation rod is connected to the bottom of the interface, the bottom end of the soft inoculation rod is provided with a smooth round end, the outer surface of the soft inoculation rod is provided with a groove, the groove is formed in the axial direction of the soft inoculation rod, the middle and upper portion of the soft inoculation rod is provided with an easily-bent ring, the easily-bent ring is arranged in the circumferential direction of the soft inoculation rod, and the diameter of the easily-bent ring is smaller than that of the soft inoculation rod. The invention further provides a microbionation method through the microbionation device. The microbionation device can serve as an inoculating loop for plate streaking inoculation and can also serve as a spreader for spread inoculation and is safe, sterile, convenient, simple, practical, skilled, simple in structure and low in production cost.

The invention relates to the technical field of microbial culture, and in particular to relates a complex microbial inoculant and an application thereof. The complex microbial inoculant is composed ofXiaoqu and red yeast rice in a mass ratio of 5: 5, wherein the Xiaoqu is composed of rhizopus and yeast in a mass ratio of 10: 1. The complex microbial inoculant is used for brewing wine, and is specifically used as distiller's yeast in the wine brewing process. The complex microbial inoculant is mainly used for brewing rice-flavor liquor, and can effectively improve the content of flavor substances such as total acid and total ester of rice-flavor liquor. The improved flavor substances just make up for the defects of taste and aroma in the past.

The invention relates to a culture medium suitable for clostridium difficile, and a preparation method thereof, and belongs to the technical field of microbial cultivation. The preparation method is used for solving a problem in the prior art that culturing effect of conventional culture medium on clostridium difficile is poor. The culture medium comprises composite ingredients such as peptone and dehydrated beef heart infusion, and also comprises complement components such as glycine and laminine. It is shown by experiment results that culture effect of the culture medium on clostridium difficile is excellent, and the culture medium possesses important implication in study on the biological characteristics of clostridium difficile and on treatment of clostridium difficile infection.

The present invention discloses a high temperature methane bacteria inoculating culture method, and belongs to the technical field of microbial culture. The basic process of the method is as follows: a basal culture medium is warmed to 25 DEG C, inoculated with methane bacteria, warmed to 35 DEG C for constant temperature culture, then warmed to 42 DEG Cfor constant temperature culture, then warmed to 45 DEG C, and then warmed to 55 DEG C for constant temperature culture. According to the method, a four-stage heating and three-stage constant temperature culture method is used to obtain high-temperature inoculating cultured methane bacteria; and the high temperature methane bacteria inoculating culture method helps to improve the number and activity of high-temperature methane bacteria, is directly applied to high-temperature fermentation methane manufacturing process, reduces the fermentation step and fermentation time of the high-temperature fermentation, and solves the problems of high energy consumption and low gas producing efficiency of high-temperature fermentation engineering in the prior art.