Culture medium suitable for clostridium difficile, and preparation method thereof

A Clostridium difficile and culture medium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as difficult growth

Active Publication Date: 2017-08-29
广西华银医学检验所有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prior art shows that this bacterium is a strictly obligate anaerob...

Method used

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  • Culture medium suitable for clostridium difficile, and preparation method thereof
  • Culture medium suitable for clostridium difficile, and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1 The present invention is applicable to the culture medium of Clostridium difficile

[0015] A culture medium suitable for Clostridium difficile, prepared from the following components in parts by weight: 10 parts of peptone, 6 parts of dehydrated calf brain extract powder, 10 parts of dehydrated beef heart extract powder, 1.0 part of glucose, 0.1 part of magnesium sulfate 1.5 parts of laminacin, 0.01 part of natamycin, 0.5 part of sodium dihydrogen phosphate, 0.5 part of glycine, 10.3 parts of vitamin B, 800 parts of purified water, and the pH was adjusted to 7.2.

[0016] The preparation method of the culture medium is as follows: measure one-half of the prescription amount of purified water, add peptone, dehydrated calf brain extract powder, dehydrated beef heart extract powder and glucose, stir to dissolve, add the remaining amount of purified water , add magnesium sulfate and sodium dihydrogen phosphate to it, adjust its pH to 7.2, sterilize at 121°C fo...

Embodiment 2

[0017] Embodiment 2 The present invention is applicable to the culture medium of Clostridium difficile

[0018] A culture medium suitable for Clostridium difficile, prepared from the following components in parts by weight: 15 parts of peptone, 8 parts of dehydrated calf brain extract powder, 15 parts of dehydrated beef heart extract powder, 1.5 parts of glucose, 0.5 parts of magnesium sulfate 2.5 parts of laminacin, 0.03 parts of natamycin, 0.9 parts of sodium dihydrogen phosphate, 0.9 parts of glycine, 10.5 parts of vitamin B, 1000 parts of purified water, and the pH was adjusted to 7.4.

[0019] The preparation method of the culture medium is as follows: measure one-half of the prescription amount of purified water, add peptone, dehydrated calf brain extract powder, dehydrated beef heart extract powder and glucose, stir to dissolve, add the remaining amount of purified water , add magnesium sulfate and sodium dihydrogen phosphate to it, adjust its pH to 7.4, sterilize at 12...

Embodiment 3

[0020] Embodiment 3 The present invention is applicable to the culture medium of Clostridium difficile

[0021] A culture medium suitable for Clostridium difficile, prepared from the following components in parts by weight: 12.5 parts of peptone, 7 parts of dehydrated calf brain extract powder, 12.5 parts of dehydrated beef heart extract powder, 1.25 parts of glucose, 0.3 parts of magnesium sulfate 2 parts of laminacin, 0.02 parts of natamycin, 0.7 parts of sodium dihydrogen phosphate, 0.7 parts of glycine, 10.4 parts of vitamin B, 900 parts of purified water, and the pH was adjusted to 7.3.

[0022] The preparation method of the culture medium is as follows: measure one-half of the prescription amount of purified water, add peptone, dehydrated calf brain extract powder, dehydrated beef heart extract powder and glucose, stir to dissolve, add the remaining amount of purified water , add magnesium sulfate and sodium dihydrogen phosphate to it, adjust its pH to 7.3, sterilize at ...

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Abstract

The invention relates to a culture medium suitable for clostridium difficile, and a preparation method thereof, and belongs to the technical field of microbial cultivation. The preparation method is used for solving a problem in the prior art that culturing effect of conventional culture medium on clostridium difficile is poor. The culture medium comprises composite ingredients such as peptone and dehydrated beef heart infusion, and also comprises complement components such as glycine and laminine. It is shown by experiment results that culture effect of the culture medium on clostridium difficile is excellent, and the culture medium possesses important implication in study on the biological characteristics of clostridium difficile and on treatment of clostridium difficile infection.

Description

technical field [0001] The invention relates to a culture medium suitable for Clostridium difficile and a preparation method thereof, belonging to the technical field of microorganism cultivation. Background technique [0002] Clostridium difficile (Clostridium difficile) is an obligate anaerobic bacterium of the genus Clostridium, which is very sensitive to oxygen and difficult to isolate and culture, hence the name. It was discovered in 1935, but it was not taken seriously until 1977 when it was discovered that this bacteria was related to pseudomembranous colitis caused by long-term clinical use of certain antibiotics (ampicillin, cephalosporin, erythromycin, clindamycin, etc.). Clostridium difficile is widely distributed in natural habitats such as soil, hay, sand, feces of some large animals (cows, donkeys, and horses), and in the feces of dogs, cats, rodents, and humans, in addition to being abundant in Clostridium difficile is often found in the feces of infants in t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12N1/02C12R1/145
CPCC12N1/02C12N1/04C12N1/20
Inventor 陈明慧王晓丹梁丽霞石秀娟
Owner 广西华银医学检验所有限公司
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