41 results about "BEEF HEART" patented technology

The invention discloses a prevotella intermedia (Pi) culture medium, which is characterized in that: a formula for preparing 1,000 ml of the culture medium contains 15g of peptone, 8g of beef heart infusion, 5 mg of protohemin, 10 mg of vitamin K1, 5.5g of sodium chloride, 15g of agar, 4 mg of doxycycline, 20 mg of metronidazole, 90 ml of sterile sheep blood and the balance being distilled water.Compared with the prior art, the Pi culture medium has the characteristics of reliability in detection and high separation rate of Pi.

The invention provides a blood agar plate, and relates to the technical field of culture media. The blood agar plate contains a plurality of substances such as casein tryptone and soy peptone. The casein tryptone, the soy peptone, yeast extract powder and beef heart infusion provide suitable carbon and nitrogen sources for bacteria; defibrillated sheep blood is rich in nutritional factors to promote the growth of common bacteria, and at the same time the hemolysis of the bacteria can be observed; sodium chloride maintains the osmotic pressure balance of the bacteria; and agar is a culture medium coagulant. The blood agar plate has more comprehensive nutrients, can effectively promote the growth of the bacteria, and improve the isolation rate of the bacteria; according to a preparation method of the blood agar plate, after a culture solution is sterilized at high temperature, cooling is performed to 80-90 DEG C, so that full gas discharging time is given to the culture solution to avoidformation of air bubbles during rapid cooling, and at the same time, before adding the defibrillated sheep blood, full preheating is performed to avoid the problem of agar agglomeration caused by toolarge temperature difference between the defibrillated sheep blood and the culture solution; and the method has simple operation and low requirements for equipment, and is suitable for industrial production.

The invention relates to an Mp (mycoplasma pneumoniae) culture and identification reagent and a preparation method thereof, belonging to the field of clinical medicine. The reagent contains a basic culture solution, standard calf serum, a yeast infusion, glucose, an Eagle culture solution, phenol red, methylene blue and penicillin, wherein the basic culture solution comprises mother liquor, NaCl, tryptone and Na2HPO4. The preparation method of the reagent comprises the steps of extracting a beef heart infusion, extracting the yeast infusion, preparing the basic culture solution, and then dissolving and mixing with other components to prepare the reagent. A rapid growth factor, an antibacterial agent and a composite indicator are added in the reagent, so that the characteristics of culture specificity, rapid propagation and high-resolution color changes of the reagent are enhanced, and the detection time is shortened to 3 hours. The Mp culture and identification reagent and the preparation method thereof, disclosed by the invention, have an important reference value and guiding significance in meeting the rapid screening and auxiliary diagnosis of suspected cases of Mp mycoplasma pneumoniae infection.

The invention discloses a biphasic media for genitourinary tract mycoplasma, and belongs to the field of microbiological detection. The biphasic media comprises a liquid medium and a solid medium, wherein the solid medium is arranged in a container and forms an inclined plane; meanwhile 1/4 to 1/2 of the inclined plane is immersed into the liquid medium; the biphasic media mainly comprises the following components: yeast extract powder, beef heart infusion, soy peptone, sodium chloride, dipotassium phosphate, manganese sulfate, cysteine hydrochloride, urea, arginine, glutamine, equinum serum,a DMEM liquid medium, sodium pyruvate, a phenol red indicator, nysfungin, penicillin and agar; the container further comprises a sealing component capable of sealing the inner space of the container and a filter screen; a carbon dioxide producing piece is arranged in the filter screen, which can absorb moisture during culturing and release carbon dioxide, so that carbon dioxide incubators are notrequired any more. The whole process is simple to operate, the specificity is high, and laboratory operation is easy.

The invention relates to a culture medium suitable for clostridium difficile, and a preparation method thereof, and belongs to the technical field of microbial cultivation. The preparation method is used for solving a problem in the prior art that culturing effect of conventional culture medium on clostridium difficile is poor. The culture medium comprises composite ingredients such as peptone and dehydrated beef heart infusion, and also comprises complement components such as glycine and laminine. It is shown by experiment results that culture effect of the culture medium on clostridium difficile is excellent, and the culture medium possesses important implication in study on the biological characteristics of clostridium difficile and on treatment of clostridium difficile infection.

Provided is a ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit. The kit can complete detection in 8-24 h, and meanwhile, drug-resistant detection of various antibiotics can be performed. The combined rapid culture kit is mainly composed of pork stomach digestive juice or lung digestive juice, a beef extracting solution or a beef heart extracting solution, cattle serum or horse serum or other animal serum, sodium chloride, peptone, yeast powder, urea, L-arginine, phenol red indicator or cresol red indicator, and penicillin or other antibiotics. The drug detection kit can detect doxycycline hyclate, roxithromycin, acetylspiramycin, gatifloxacin, clarithromycin, azithromycin, clindamycin, erythromycin, kitasamycin, ofloxacin, levofloxacin hydrochloride, sparfloxacin, ciprofloxacin, doxycycline, erythromycin cydocarbonate and various other antibiotics. By means of the detection kit, not only is the mycoplasma detection time shortened, but also drug-resistant detection can be performed at the same time so that clinical medication can be guided. The detection kit has the beneficial effects of being high in sensitivity, high in specificity, high in detection rate, easy and convenient to operate and the like.

The invention discloses a nutrient solution for detecting bacterial vaginosis. Each 100ml of nutrient solution contains the following raw materials: 0.1-1g of beef heart powder, 0.5-5g of tryptone, 0.1-2g of glucose, 0.1-1g of sodium chloride, 0.1-1g of bromocresol purple sodium, 5-20ml of horse serum, 0.0001-0.001g of neomycin sulfate and 0.0001-0.001g of nystatin. The invention further discloses a kit for detecting bacterial vaginosis and a detection method. The detection kit is stable in quality; the detection method is simple in operation and accurate in result. The kit has the functions of target cultivation and simultaneous detection of the sensitivity of a drug, the detection method is completed in one step within 6-24h, the success rate is high, the drug sensitivity result is reliable, clinical accurate diagnosis of the bacterial vaginosis and the drug sensitivity test can be met, a reference foundation is provided for a clinician in timely accurate diagnosis and reasonable use of the drug, and clinical abuse of antibiotics is avoided.

The invention discloses a famous Hakka dish Shuanjiumentou with a health function and a cooking method thereof. The cooking method comprises the following steps: finely selecting meat of most fine nine parts of cattle which is killed at the moment as nine jiumentou materials, that is, omasum, cattle belly, beef omasum, oxtongue, beef chine, beef hearts, beef kidneys, beef intestines and beef livers, strictly and finely treating, rinsing in boiled Hakka watery wine prepared from schisandraceae, sarcandrahainanensis and litsea cubeba together, fishing out the materials after the watery wine in a pot is reboiled for 0.5-1.5 minutes, putting the materials into a disc, adding smashed ginger, chopped shallot stalk, fresh domestic lard oil, salt and aginomoto into the disc, and uniformly stirring, thereby obtaining the famous Hakka dish Shuanjiumentou which is fragrant in smell, capable of prompting the appetite, fresh and smooth and crispy, long-lasting in taste, fragrant and not greasy and long-lasting in attractiveness and has the health function.

The invention discloses a culture medium capable of quickly and accurately detecting listeria monocytogenes and a detection method. The disclosed culture medium comprises the following components: soya bean lecithin, agar, Casein tryptone, glucose, soy peptone, dipotassium phosphate, yeast powder, beef heart powder, sodium chloride, lithium chloride, active carbon powder and distilled water. The detection method comprises the following steps: preparing the components into the culture medium according to a method, then selecting a sample to firstly carry out LB1 pre-enrichment and listeria monocytogenes enrichment and then inoculate the culture medium prepared in the invention, after the culture is finished, selecting bacterial colonies generating settling rings, and carrying out purification and bacterium identification according to the national standard GB4789.30-2016. The culture medium has the main advantages that the settling rings for listeria monocytogenes appear at early time, and the detection time is shortened. The detection rate, specificity and false positive rate are equivalent to those in national standard, and the listeria monocytogenes can be detected effectively. According to the detection method disclosed by the invention, the period is greatly shortened compared with the national standard, and the method is free of significant difference.

The invention relates to a mycoplasma synoviae culture medium and a preparation method thereof, and belongs to the technical field of veterinary biology. The mycoplasma synoviae culture medium is composed of a basic culture medium and an auxiliary culture medium, the basic culture medium mainly comprises Hank's concentrated solution, milk protein hydrolysate, yeast extract, beef heart extract powder and trehalose, and a high-pressure steam sterilization mode is used for sterilization; the auxiliary culture medium is prepared from arginine, sodium pyruvate, coenzyme I, penicillin and porcine serum, and all the components of the auxiliary culture medium are filtered and sterilized. The titer of the mycoplasma synoviae cultured by the culture medium disclosed by the invention reaches 1010-1011CCU/ml, the culture time is 14-24h, the content of porcine serum is as low as 5%, and the stress reaction of heterologous to chickens and the production cost of enterprises are greatly reduced. The inactivated vaccine prepared from the mycoplasma synoviae cultured by the culture medium has good safety and immunogenicity.

The invention discloses a symphysodon aequifasciata feed. Based on the weight of the feed, the prepared feed comprises 0.01-99% of grass worm powder, 0.01-50% of banana peel extract and potassium selenite. The feed preferably further comprises 3-7% of carbohydrate, 10-45% of proteins, 5-15% of fat and added vitamins and amino acids. The invention also discloses a preparation method of the symphysodon aequifasciata feed. The feed has the beneficial effects that a common beef heart hamburger feed is replaced by tylorrhynchus powder and shrimp meat powder, and grease rich in linolenic acid and linoleic acid is used as a fat source which matches with nutrient substances such as the green banana peel extract rich in 5-hydroxytryptophan, sodium selenite, amino acids, vitamins and minerals The feed can provide balanced nutrients, essential amino acids and essential fatty acids for symphysodon aequifasciata, promote gastrointestinal enzyme activity of symphysodon aequifasciata, increase appetite, and promote growth. The symphysodon aequifasciata feed can be preserved for a long time, and the complicated steps that the symphysodon aequifasciata feeds in the prior art need to be prepared onthe site and freezing storage are avoided.

The invention discloses a beef offal sausage product containing sheep tendons, and a preparation method thereof. The preparation method of the product comprises the following steps: mixing, by mass, 50 parts of beef internal organs, 2-4 parts of sheep tendons, 2-3 parts of an alfalfa slurry and 1-2 parts of a kelp slurry to obtain a main material; and adding auxiliary materials and 8 parts by massof water to the main material, performing stirring until the obtained filling is uniform, pickling the filling at 4 DEG C for 12 h to obtain a pickled material, filling a collagen casing with the pickled filling, carrying out low-temperature bonding, steaming the casing filled with the filling for 35-40 min, cooling the steamed casing filled with the filling to room temperature, brushing the casing with a layer of honey, and baking the brushed casing at 120-150 DEG C for 10 min to obtain the beef offal sausage product containing sheep tendons, wherein the beef internal organs comprises beef hearts and beef livers according to a mass ratio of (3-5):1. The beef sausage product has the characteristics of no fishy smell, good taste, matching of vegetables and meat, dietary balance and comprehensive nutrition, and can prevent osteoporosis, bone fracture and arthrosis of the elderly; and the utilization way of the beef hearts and beef livers in beef byproducts is enlarged, and the added values are improved.

The invention discloses a mycoplasma culture medium and a preparation method thereof. The invention is characterized in that the formula of preparing 1000ml of culture medium contains 15g of peptone, 8g of beef heart infusion, 5g of yeast extract powder, 20mg of phenol red, 5.2g of sodium chloride, 0.5g of L-arginine, 30mg of fluconazole, 2mg of purpureacin, 200ml of sterile sheep blood and the balance of distilled water. Compared with the prior art, the invention has the advantages of reliable detection and high mycoplasma separation rate.

The invention discloses a candida selective culture medium for a feces specimen and a preparation method thereof. The invention is characterized in that every 1000ml of culture medium contains malt extract powder, beef heart powder, magnesium sulfate, agar, gentamicin sulphate, aniline blue, calcium citrate tetrahydrate and the balance of distilled water. The preparation method comprises the following steps: dissolving the malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, gentamicin sulphate, allithiamine and glycocholic acid in an orchis saina turcz decoction, evenly mixing, diluting to the specified volume, sterilizing and subpackaging. Compared with the prior art, the invention has the characteristics of high detection reliability and high candida separation rate.

The invention provides carassius auratus feed added with astragalus polysaccharide and a preparation method thereof. The carassius auratus feed is characterized by being prepared from the following components in parts by weight: 40 to 60 parts of corn starch, 10 to 20 parts of soybean meal, 10 to 15 parts of starch, 6 to 10 parts of flour, 30 to 40 parts of fish meal, 20 to 30 parts of brine shrimp, 10 to 20 parts of water flea, 10 to 20 parts of beef heart, 6 to 10 parts of yeast powder, 8 to 15 parts of fish oil, 0.5 to 1 part of the astragalus polysaccharide and 0.005 to 0.008 part of multivitamin. According to the formula provided by the invention, the added astragalus polysaccharide can be used for improving the immunity and anti-stress capability of carassius auratus; the astragaluspolysaccharide and astaxanthin in the brine shrimps and the water fleas are mutually matched to use, so that the morbidity of the carassius auratus can be reduced, the stress resistance of the carassius auratus is increased and the economic benefits of farmers are ensured very well.

The invention discloses a candida selective culture medium for a feces specimen and a preparation method thereof. The invention is characterized in that every 1000ml of culture medium contains malt extract powder, beef heart powder, magnesium sulfate, agar, gentamicin sulphate, aniline blue, calcium citrate tetrahydrate and the balance of distilled water. The preparation method comprises the following steps: dissolving the malt extract powder, beef heart powder, magnesium sulfate, agar, aniline blue, calcium citrate tetrahydrate, gentamicin sulphate, allithiamine and glycocholic acid in an orchis saina turcz decoction, evenly mixing, diluting to the specified volume, sterilizing and subpackaging. Compared with the prior art, the invention has the characteristics of high detection reliability and high candida separation rate.

The present invention discloses a Chongqing style boiled blood curd instant dish package and a preparation method thereof, and relates to the technical field of food processing. The Chongqing style boiled blood curd instant dish package comprises the following raw materials in parts by weight: 180-220 parts of duck blood, 45-55 parts of beef heart, 35-50 parts of agaric, 28-36 parts of sliced winter bamboo shoots, 45-55 parts of beef tripe and 220-250 parts of Chongqing style boiled blood curd sauce. The instant dish package is processed by five processes of food material pretreatment, stir-frying of hairy blood hyperactivity seasoning and sauce mixing, packaging according to a proportion, sterilization treatment or quick-freezing treatment and detection. According to the invention, the prepared instant dish is spicy and fragrant, rich in juice and flavor, rich in nutrition, and has a certain health-care effect. Moreover, the instant dish package is adapted to different heating methods. After heating, the form and taste of the materials are kept intact, the blood clots are not easy to generate a honeycomb phenomenon, the duck blood flavor is elastic and smooth, winter bamboo shootsand agaric are refreshing and greasy, the beef heart is soft and glutinous in mouth, and the beef tripe is firm and chewy. The instant dish package has stable flavor and is particularly popular withpeople.

The invention discloses a poecilia reticulata feed and a preparation method thereof. The poecilia reticulata feed is prepared from the following raw materials in parts by weight: 100-150 parts of deionized water, 60-80 parts of corn flour, 10-15 parts of bean cake flour, 5-10 parts of wheat flour, 15-20 parts of bran, 5-10 parts of oat flour, 20-50 parts of fish meal, 20-60 parts of brine shrimps, 10-15 parts of threadworms, 5-10 parts of turmbicula akamushi, 30-50 parts of beef hearts, 15-30 parts of earthworms, 10-30 parts of tubifex hattai, 5-15 parts of water fleas, 20-40 parts of lettuces, 15-30 parts of garlic, 10-20 parts of calcium hydrogen phosphate, 15-30 parts of spirulina, 12-15 parts of lecithin, 15-30 parts of multivitamins, 10-30 parts of fish oil and 2-5 parts of refined salt. According to the poecilia reticulata feed and the preparation method thereof disclosed by the invention, reasonable raw materials and reasonable compounding ratios are selected, so that various nutritional requirements required for growth of poecilia reticulata are met, and the poecilia reticulata after being fed with the poecilia reticulata feed for a long term can be bright in color, high in born number and long in life.