Mp (mycoplasma pneumoniae) culture and identification reagent and preparation method thereof

A technology of mycoplasma pneumoniae and reagents, applied in the field of clinical medicine, to achieve the effects of shortened detection time, reasonable nutritional content, and enhanced culture specificity

Inactive Publication Date: 2014-06-11
武汉菁华时间科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Mp mycoplasma rapid culture identification kit and preparation method of the present invention have a detection time of 3 hou...

Method used

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  • Mp (mycoplasma pneumoniae) culture and identification reagent and preparation method thereof
  • Mp (mycoplasma pneumoniae) culture and identification reagent and preparation method thereof
  • Mp (mycoplasma pneumoniae) culture and identification reagent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A Mycoplasma pneumoniae culture identification reagent, the mass percentage of each component in the reagent is as follows:

[0032]

[0033]

[0034] The reagent also includes 150,000 units / 100ml of penicillin.

[0035] Wherein, the mass percentage of each component in described basal culture fluid is as follows:

[0036]

Embodiment 2

[0038] A Mycoplasma pneumoniae culture identification reagent, the mass percentage of each component in the reagent is as follows:

[0039]

[0040] The reagent also includes 200,000 units / 100ml of penicillin.

[0041] Wherein, the mass percentage of each component in described basal culture fluid is as follows:

[0042]

Embodiment 3

[0044] A Mycoplasma pneumoniae culture identification reagent, the mass percentage of each component in the reagent is as follows:

[0045]

[0046] The reagent also includes 100,000 units / 100ml of penicillin.

[0047] Wherein, the mass percentage of each component in described basal culture fluid is as follows:

[0048]

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PUM

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Abstract

The invention relates to an Mp (mycoplasma pneumoniae) culture and identification reagent and a preparation method thereof, belonging to the field of clinical medicine. The reagent contains a basic culture solution, standard calf serum, a yeast infusion, glucose, an Eagle culture solution, phenol red, methylene blue and penicillin, wherein the basic culture solution comprises mother liquor, NaCl, tryptone and Na2HPO4. The preparation method of the reagent comprises the steps of extracting a beef heart infusion, extracting the yeast infusion, preparing the basic culture solution, and then dissolving and mixing with other components to prepare the reagent. A rapid growth factor, an antibacterial agent and a composite indicator are added in the reagent, so that the characteristics of culture specificity, rapid propagation and high-resolution color changes of the reagent are enhanced, and the detection time is shortened to 3 hours. The Mp culture and identification reagent and the preparation method thereof, disclosed by the invention, have an important reference value and guiding significance in meeting the rapid screening and auxiliary diagnosis of suspected cases of Mp mycoplasma pneumoniae infection.

Description

technical field [0001] The invention relates to a reagent for culturing and identifying mycoplasma pneumoniae and a preparation method thereof, belonging to the field of clinical medicine. technical background [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae Mp) is a newly discovered pathogenic microorganism. It is transmitted by air droplets from the patient's mouth and nose secretions, and it is easy to cause sporadic or regional epidemics. Its main symptom is respiratory tract infection with pneumonia, namely Mycoplasma pneumoniae pneumonia MPP. It has obvious effects on humans. pathogenicity. Mp infection can occur in all seasons, but mostly in autumn and winter. Infants and adolescents are susceptible groups. The incidence rate is 20-30% of that of children with pneumonia. It is densely populated and the epidemic season can reach 50%, and the epidemic cycle is 3-7 years. In recent years, there is a trend of shortening the epidemic cycle and increasing the inciden...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 杨会宣牛涛
Owner 武汉菁华时间科技有限公司
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