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Prevotella intermedia (Pi) culture medium

A technology of Prevotella intermedia and culture medium, which is applied in the field of Prevotella intermedia culture medium, can solve the problems of difficulty in separating and affecting the growth of Prevotella intermedia, and achieves the effect of high separation rate and reliable detection.

Inactive Publication Date: 2012-07-04
刘名霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to isolate anaerobic streptococcus, Porphyrophyllum gingivalis, Clostridium spp., etc. when culturing tartar specimens with non-selective anaerobic bacteria basal medium, which affects the growth of Prevotella intermedia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1, the formula for preparing 1000ml medium contains: peptone 15g; beef heart extract powder 8g; hemin 5mg; vitamin K1 10mg; sodium chloride 5.5g; agar 15g; doxycycline 4mg; metronidazole 20mg; sterile sheep blood 90ml; add distilled water to 1000ml. The preparation method is to weigh peptone, beef heart extract powder, sodium chloride; agar; doxycycline; metronidazole, mix evenly, dissolve in distilled water, sterilize at 210°C for 15 minutes, cool to 45°C, add sterile sheep blood, Sterilized hemin, sterilized vitamin K1, mixed evenly, sterilized distilled water and distilled to 1000ml before packing.

Embodiment 2

[0013] Example 2, the formula for preparing 1000ml medium contains: peptone 15g; beef heart extract powder 8g; hemin 5mg; vitamin K1 10mg; sodium chloride 5.5g; agar 15g; doxycycline 4mg; metronidazole 20mg; sterile sheep blood 90ml; bacitracin zinc 2mg; add distilled water to 1000ml. The preparation method is to weigh peptone, beef heart extract powder, sodium chloride; agar; doxycycline; metronidazole; Bacterial sheep blood, sterilized hemin, sterilized vitamin K1, mixed evenly, sterilized distilled water and distilled to 1000ml before packing.

Embodiment 3

[0014] Example 3, the formula for preparing 1000ml medium contains: peptone 15g; beef heart extract powder 8g; hemin 5mg; vitamin K1 10mg; sodium chloride 5.5g; agar 15g; doxycycline 4mg; metronidazole 20mg; sterile sheep blood 90ml; L-glutamine 0.1g; bile salt 1.8g; add distilled water to 1000ml. The preparation method is to weigh peptone, beef heart extract powder, sodium chloride; agar; doxycycline; metronidazole; L-glutamine; At 45°C, add sterile sheep blood, sterilized hemin, and sterilized vitamin K1, mix well, dilute to 1000ml with sterilized distilled water, and dispense.

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PUM

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Abstract

The invention discloses a prevotella intermedia (Pi) culture medium, which is characterized in that: a formula for preparing 1,000 ml of the culture medium contains 15g of peptone, 8g of beef heart infusion, 5 mg of protohemin, 10 mg of vitamin K1, 5.5g of sodium chloride, 15g of agar, 4 mg of doxycycline, 20 mg of metronidazole, 90 ml of sterile sheep blood and the balance being distilled water.Compared with the prior art, the Pi culture medium has the characteristics of reliability in detection and high separation rate of Pi.

Description

technical field [0001] The invention relates to the field of culture of microorganisms in the oral cavity, in particular to an intermediate Prevotella culture medium. Background technique [0002] Periodontal disease is a common oral disease with a high incidence worldwide. Prevotella intermedia (Pi) is a melanin-producing G-anaerobic bacterium. A large number of studies have shown that Pi is closely related to infectious diseases such as periodontitis, periapical disease and pericoronitis. The current Pi test method is mainly subgingival plaque sampling for polymerase chain reaction (PCR), which requires DNA extraction and PCR primer design for the sample, and requires PCR-related instruments and equipment, which is expensive , the operation is complex. Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, et...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 刘名霞庞渤
Owner 刘名霞
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