Blood agar plate and preparation method thereof

Abstract

The invention provides a blood agar plate, and relates to the technical field of culture media. The blood agar plate contains a plurality of substances such as casein tryptone and soy peptone. The casein tryptone, the soy peptone, yeast extract powder and beef heart infusion provide suitable carbon and nitrogen sources for bacteria; defibrillated sheep blood is rich in nutritional factors to promote the growth of common bacteria, and at the same time the hemolysis of the bacteria can be observed; sodium chloride maintains the osmotic pressure balance of the bacteria; and agar is a culture medium coagulant. The blood agar plate has more comprehensive nutrients, can effectively promote the growth of the bacteria, and improve the isolation rate of the bacteria; according to a preparation method of the blood agar plate, after a culture solution is sterilized at high temperature, cooling is performed to 80-90 DEG C, so that full gas discharging time is given to the culture solution to avoidformation of air bubbles during rapid cooling, and at the same time, before adding the defibrillated sheep blood, full preheating is performed to avoid the problem of agar agglomeration caused by toolarge temperature difference between the defibrillated sheep blood and the culture solution; and the method has simple operation and low requirements for equipment, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a blood agar plate and a preparation method thereof. Background technique [0002] Pathogenic bacteria or opportunistic pathogenic bacteria exist in the infected site, leading to common clinical infections, such as respiratory tract infection, reproductive tract infection, intestinal infection, etc. In order to accurately diagnose the type of bacteria in the infected site, patients are often used clinically Samples are collected from the site for culture, and clinical examiners select suspicious strains for identification based on clinical symptoms, so as to determine the type of infected bacteria in the patient's site and provide a basis for clinical treatment. Diagnosis and culture of infected bacterial types in patient parts are currently the only feasible methods in clinical diagnosis. Other methods, such as immune antigen detection and nucleic acid detection, are not ...

AI Technical Summary

Problems solved by technology

The nutritional content is not very comprehensive and uniform, and the ability to repair damaged bacteria is poor, so it is difficult to meet more and more stringent tests

Moreover, the existing blood agar preparation method is more suitable for small-scale self-production and self-use. During large-scale production, there will be agglomeration, entrained air bubbles, etc., which will affect product quality.