Blood agar plate and preparation method thereof

A blood agar plate and agar technology are applied in the field of blood agar plate and its preparation, which can solve the problems of caking, entrapped air bubbles, and difficulty in detection, and achieve the effects of improving separation rate, promoting growth and comprehensive nutrition.

Inactive Publication Date: 2020-02-21
中秀科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nutritional content is not very comprehensive and uniform, and the ability to repair damaged bacteria is poor, so it is difficult to meet more and more stringent tests
Moreover, the existing blood ag...

Method used

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Examples

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preparation example Construction

[0029] The embodiment of the present invention also provides a kind of preparation method of above-mentioned blood agar plate, it comprises:

[0030] S1. Mix tryptone, soybean peptone, sodium chloride, yeast extract powder, beef heart extract powder, agar, and water, heat to dissolve, and obtain a culture solution.

[0031] S2. Sterilize the culture medium at 120-125°C for 20-40 min.

[0032] S3. Cool down the sterilized culture solution to 80-90°C, continue heating for 5-10 minutes, then cool to 40-45°C;

[0033] S4. Mix the preheated defibrated sheep blood with the cooled culture medium evenly, pour it into a plate for further cooling, and obtain a blood agar plate.

[0034] Further, tryptone, soybean peptone, yeast extract powder, and beef heart extract powder provide suitable carbon and nitrogen sources for microorganisms. Sodium chloride can maintain the balance of bacterial osmotic pressure. Agar is the medium coagulant. At room temperature, the dissolution of the ab...

Embodiment 1

[0042] This embodiment provides a blood agar plate, which is obtained by mixing culture fluid and defibrated sheep blood, cooling and solidifying. Wherein, in parts by weight, the culture solution includes:

[0043] 8 parts of tryptone, 10 parts of soybean peptone, 4 parts of sodium chloride, 4 parts of yeast extract powder, 1 part of beef heart extract powder, 13 parts of agar, and 1000 parts of water;

[0044] The volume ratio of culture medium and defibrated sheep blood is 1:0.06.

[0045] The preparation method of this blood agar plate is as follows:

[0046] S1. Mix tryptone, soybean peptone, sodium chloride, yeast extract powder, beef heart extract powder, agar, and water, heat to 90°C to dissolve to obtain a culture medium, and adjust the pH of the culture medium to 7.2.

[0047]S2. Sterilize the culture medium at 120°C for 40 min.

[0048] S3. Cool down the sterilized culture solution to 90°C, continue heating for 5 minutes, then cool to 40°C;

[0049] S4. Mix the ...

Embodiment 2

[0053] This embodiment provides a blood agar plate, which is obtained by mixing culture fluid and defibrated sheep blood, cooling and solidifying. Wherein, in parts by weight, the culture solution includes:

[0054] 12 parts of tryptone, 6 parts of soybean peptone, 6 parts of sodium chloride, 2 parts of yeast extract powder, 3 parts of beef heart extract powder, 18 parts of agar, and 900 parts of water;

[0055] The volume ratio of culture medium and defibrated sheep blood is 1:0.04.

[0056] The preparation method of this blood agar plate is as follows:

[0057] S1. Mix tryptone, soybean peptone, sodium chloride, yeast extract powder, beef heart extract powder, agar, and water, heat to 80°C to dissolve to obtain a culture medium, and adjust the pH of the culture medium to 7.4.

[0058] S2. Sterilize the culture medium at 125°C for 20 min.

[0059] S3. Cool down the sterilized culture solution to 80°C, continue heating for 10 minutes, then cool to 45°C;

[0060] S4. Mix th...

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Abstract

The invention provides a blood agar plate, and relates to the technical field of culture media. The blood agar plate contains a plurality of substances such as casein tryptone and soy peptone. The casein tryptone, the soy peptone, yeast extract powder and beef heart infusion provide suitable carbon and nitrogen sources for bacteria; defibrillated sheep blood is rich in nutritional factors to promote the growth of common bacteria, and at the same time the hemolysis of the bacteria can be observed; sodium chloride maintains the osmotic pressure balance of the bacteria; and agar is a culture medium coagulant. The blood agar plate has more comprehensive nutrients, can effectively promote the growth of the bacteria, and improve the isolation rate of the bacteria; according to a preparation method of the blood agar plate, after a culture solution is sterilized at high temperature, cooling is performed to 80-90 DEG C, so that full gas discharging time is given to the culture solution to avoidformation of air bubbles during rapid cooling, and at the same time, before adding the defibrillated sheep blood, full preheating is performed to avoid the problem of agar agglomeration caused by toolarge temperature difference between the defibrillated sheep blood and the culture solution; and the method has simple operation and low requirements for equipment, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of culture medium, in particular to a blood agar plate and a preparation method thereof. Background technique [0002] Pathogenic bacteria or opportunistic pathogenic bacteria exist in the infected site, leading to common clinical infections, such as respiratory tract infection, reproductive tract infection, intestinal infection, etc. In order to accurately diagnose the type of bacteria in the infected site, patients are often used clinically Samples are collected from the site for culture, and clinical examiners select suspicious strains for identification based on clinical symptoms, so as to determine the type of infected bacteria in the patient's site and provide a basis for clinical treatment. Diagnosis and culture of infected bacterial types in patient parts are currently the only feasible methods in clinical diagnosis. Other methods, such as immune antigen detection and nucleic acid detection, are not ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 孙月鹏张娟丽吕斌谭韦丽罗江卫
Owner 中秀科技股份有限公司
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