48 results about "Bacterial types" patented technology

The present invention provides analytical methods and related kits for detecting the presence of bacteria in a sample, determining the amount of bacteria in a sample, or determining the type of bacteria in a sample. In particular, the invention relates to devices and methods suitable for the rapid detection, quantification, or identification of bacteria in a liquid sample by measuring bacterial ATP activity.

The present invention discloses a novel quinobenzoxazine self-assembly complex on DNA and on the topoisomerase II-DNA complex. The related model is used to design a new series of quinobenzoxazines, pyridobenzophenoxazines, pyrridonaphthophenoxazines, and other related compounds that may exhibit anticancer or antibiotic activity. The anticancer activity of these compounds is thought to operate via stabilization of the topoisomerase II-DNA complex and / or interaction with G-quadruplexes, while the antibiotic activity of these compounds derives from their ability to inhibit gyrase, the bacterial type II topoisomerase.

To provide a cholesterolemia treatment agent comprising, as an active ingredient, a bacterial mixture of three bacterial types consisting of lactic acid bacteria, butyric acid bacteria, and glycation bacteria which are effective in lowering serum cholesterol. And to provide a triglyceridemia treatment agent comprising, as an active ingredient, a bacterial mixture of three bacterial types consisting of lactic acid bacteria, butyric acid bacteria, and glycation bacteria which are effective in lowering triglycerides.

Methods and kits for sampling mucous from within a sinus to determine if a single sample includes one or more bacterial types indicating bacterial sinusitis.

The invention discloses a buffering type energy accumulator and relates to an energy accumulator. A capsule is arranged in a shell, one end of the capsule is connected with a heavy charging valve through a gas valve rod, a protection cap is arranged on the outer portion of the gas valve rod, the outer portion of the gas valve rod is fixed to one end of the shell through a round nut, a nameplate isfurther arranged between the round nut and the shell, the other end of the shell is fixed to a flow valve body through a supporting ring, a rubber ring, a metal check ring, an O-shaped ring, an F4 check ring, a pressing ring and a locking nut, a piston is arranged in the middle of the flow valve body in a penetrating mode, a bacterial type valve is arranged at the inner end of the piston, a spring is further arranged on the outer portion of the bacterial type valve, a disc spring is arranged on the outer portion of the piston, and the outer end of the piston is connected with the self-lockingnut through a flat gasket. The buffering type energy accumulator is reasonable in structural design, good in buffering effect, low in maintaining and replacing cost and high in practicability, and the service life of the energy accumulator is greatly prolonged.

Apparatuses for sampling mucous from within a sinus to determine if a single sample includes one or more bacterial types indicating bacterial sinusitis comprising an indicator to guide the angle at which a user inserts the apparatus relative to the patient.

The present invention relates to a method for detecting substances specifically inhibiting a type III secretion mechanism and functions of the type III secretory proteins, within short time and large amounts thereof, without depending upon animal infectious experiments. Namely it relates to the method for detection of a type III secretory mechanism inhibitor comprising mixing a bacterium having the type III secretory mechanism and an erythrocyte suspension, adding the type III secretory mechanism inhibitor thereto, and detecting changes in the thus formed hemolytic activity. The method for detecting substances can be treated large amount of samples within short time by exhibiting the substances inhibiting the type III secretion mechanism or the functions of the type III secretory proteins as numerical index of the hemolytic activity of erythrocytes. Consequently, the present invention is useful for development of drugs.

The invention discloses a fermented milk sampling infectious microbe detection device for production. The device comprises a base and a mounting plate fixed on the base, the mounting plate is provided with a liquid strain mixing part and a gas strain culture part, the mounting plate is provided with a constant temperature box for culturing a strain culture mixed solution at a constant temperature, the mounting plate is provided with a detection part used for observing the number of infectious microbe colonies, the liquid strain mixing part is used for mixing fermented milk liquid and a culture solution, the mixed culture mixed solution is subjected to constant-temperature culture in a constant-temperature box, and the gas strain culture part is used for performing constant-temperature culture on a mixed solution of gas and the culture solution, and the detection part is used for observing the colony count of the liquid strain mixed solution and the gas strain mixed solution, observing whether bacterial contamination exists or not and judging the bacterial type. The device can perform pretreatment and detection on a sample, the whole process is continuous, sampling detection of infectious microbe by experimenters is greatly facilitated, and efficiency is high.

The invention provides a method for constructing a clinical mastitis model of two bacterial types in different udder regions of dairy cows. The method comprises the following steps: selecting a Chinese Holstein dairy cow as the experimental animal, wherein the selected dairy cow is healthy, does not have disease history, is normal in body condition, has excellent breast development and is at the middle stage of lactation for the first parity, SCC is less than100000 per mL, and results of milk bacterial culture in all udder regions are negative; respectively carrying out the following treatment on four udder regions of the dairy cow: injecting 1*10<7>CFU / mL of staphylococcus aureus bacterial suspension and 1*10<6> CFU / mL of streptococcus suspension into breasts of the dairy cow via ductus papillaris by using a breast clearing needle, injecting sterile PBS in equal amount for the control group, and not carrying out treatment on a blank group; and reversely pressing the breasts from the base of each nipple from bottom to top with hands in a way that the thumbs and the index fingers are connected into a circle so that the bacterial suspensions can uniformly diffuse to lactiferous ducts and do not accumulate in gland cisterns; and attacking with bacteria for 24 hours to make sure that the dairy cow mastitis model is successfully constructed. According to the method, the influence of individual difference on tests can be completely eliminated.

Methods and antibody compositions that displace mannose from the binding pocket of fimbrial adhesin FimH of enterobacteria, including uropathogenic E. coli, Klebsiella oxytoca, or Klebsiella pneumoniae, can be used to disrupt or prevent the attachment of a single layer of bacteria to a mannose-coated surface, or to disrupt or prevent the formation of a multilayer biofilm. The antibody compositions of the invention can thus be used in methods to inhibit, prevent, or reverse the colonization of a surface with enterobacteria that express the bacterial Type 1 fimbrial adhesin FimH, to inhibit or prevent infection of a cell by enterobacteria that express the bacterial Type 1 fimbrial adhesin FimH, such as, for example, uropathogenic E. coli, to treat a bacterial infection in subject in need thereof and to treat or prevent inflammatory bowel disease (IBD), among other uses.

The invention relates to a construction method for broad spectrum bacteria host green fluorescent protein expression vector, belonging to microbiology field. The technical proposal is that an Amp promoter is connected with a green fluorescent protein structural gene by a PCR amplified ampicillin resistance gene, and a broad spectrum type prokaryotic expression vector is obtained to start the green fluorescent protein expression. The expression vector of ampicillin resistance gene is used to construct the broad spectrum type GFP expression vector, which can widely affect the bacteria. The constructed vector expression to GFP prohibits the limitation of bacterial types and species, and bacterial growing environment; once the constructed GFP expression vector is transformed into the bacteria cells, the constructed GFP expression vector plays a role and expresses GEP, which widely extends the application field.

Methods and kits for sampling mucous from within a sinus to determine if a single sample includes one or more bacterial types indicating a bacterial infection, such as bacterial sinusitis, and one or more viruses indicating a viral infection, such as influenza.

The invention discloses a 16-valent streptococcus pneumoniae conjugate vaccine composition. The vaccine composition is obtained in a manner that streptococcus pneumoniae capsular polysaccharide afterseparation and purification and carrier protein are subjected to coupling combination to be mixed, wherein the capsular polysaccharide includes 16 streptococcus pneumoniae serotypes of 1, 2, 3, 4, 5,6A, 6B, 7F, 9V, 11A, 14, 15B, 18C, 19A, 19F and 23F. The 16-valent streptococcus pneumoniae conjugate vaccine composition can cover most of common pathogenic bacterial types or drug-resistance bacterial types of our country, and has the broad-spectrum and efficient protection effect on high-risk susceptible populations such as new-born infants, old people and children under two years old.

Methods and kits for sampling mucous from within a sinus to determine if a single sample includes one or more bacterial types indicating bacterial sinusitis.

Organic compounds showing the ability to inhibit effector toxin secretion or translocation mediated by bacterial type III secretion systems are disclosed. The disclosed type III secretion system inhibitor compounds are useful for combating infections by Gram-negative bacteria such as Salmonella spp., Shigella flexneri, Pseudomonas spp., Yersinia spp., enteropathogenic and enteroinvasive Escherichia coli, and Chlamydia spp. having such type III secretion systems.

The invention provides a coenzyme Q10 fermentation process and a control strategy. Rhodobacter sphaeroides fermentation is adopted to produce coenzyme Q10, a specific operation method of coenzyme Q10 fermentation is provided, a process regulation and control standard is created, through bacterial type change in observation of the fermentation process, the bacterial type transition period is taken as a basis for judging time and amplitude for process parameter adjustment, the fermentation level of the coenzyme Q10 is greatly improved, the production level is stabilized, and the fermentation cost is reduced.

The invention relates to application of enol substances in pine needles in the aspects of regulating and controlling a panax notoginseng rhizosphere microflora structure and / or repairing a panax notoginseng continuous cropping carrier and / or improving panax notoginseng planting. What discovers is that the quantity of fungi in panax notoginseng rhizosphere soil or panax notoginseng continuous cropping soil can be reduced and the quantity of bacteria can be obviously increased by treating potted panax notoginseng or soil by using rich substance components alpha-pinene, 2, 3-butanediol and D-pinitol in a fumigation, spraying or irrigation manner, therefore, the ratio of fungi to bacteria in the soil is remarkably reduced, and the soil is converted from a fungal type into a bacterial type, so that planting and growth of panax notoginseng are facilitated.

Organic compounds showing the ability to inhibit effector toxin secretion or translocation mediated by bacterial type III secretion systems are disclosed. The disclosed type III secretion system inhibitor compounds are useful for combating infections by Gram-negative bacteria such as Salmonella spp., Shigella flexneri, Pseudomonas spp., Yersinia spp., enteropathogenic and enteroinvasive Escherichia coli, and Chlamydia spp. having such type III secretion systems.

The invention relates to the technical field of agricultural microorganisms, in particular to a compound microbial agent for preventing potato scab, which is prepared from the following raw materials in parts by weight: 5-25 parts of bacillus subtilis, 5-25 parts of paecilomyces lilacinus, 5-25 parts of trichoderma harzianum, 5-25 parts of brevibacillus laterosporus and 48-52 parts of glucose. The compound microbial agent disclosed by the invention is strong in adaptability and good in stability, is beneficial to colonization and activity maintenance of microorganisms at roots of potatoes, has a relatively strong antagonistic effect on fungal diseases in rhizosphere soil of the potatoes, and promotes enrichment of beneficial bacteria at the rhizosphere of the potatoes, so that the rhizosphere soil of the potatoes is converted from a fungal type to a bacterial type, and the incidence rate of fungal diseases of potatoes is reduced; and meanwhile, the absorption effect and the fixation effect of potato root systems can be enhanced, rich nutrient substances are provided for growth of potato plants, and the yield and the quality of potatoes are improved.

The invention discloses a Chinese herbal medicine bacteriostatic effervescent tablet containing ligusticum wallichii extract and a preparation method thereof. The effervescent tablet mainly comprises active ingredients and auxiliary materials, the active ingredients comprise 15-30 parts by mass of ligusticum wallichii, 10-20 parts by mass of fructus gardeniae, 5-15 parts by mass of szechwan chinaberry fruit and 5-15 parts by mass of fructus. The auxiliary materials comprise an acid source, an alkali source, a lubricant, lactose and tea polyphenol. The effervescent tablet disclosed by the invention has a good inhibition effect on common bacteria which are easily contacted by daily exposure of skin. The product is convenient to carry and easy to store, and exposed skin parts such as hands and the like can be soaked and cleaned at any time after the hand-washing liquid is soaked in water, so that the cleaning and antibacterial effects are achieved. Many seasonal influenza viruses are transmitted through a hand-mouth way, and the purpose of reducing epidemic disease transmission can be achieved by using the effervescent tablets disclosed by the invention.

Organic compounds showing the ability to inhibit effector toxin secretion or translocation mediated by bacterial type III secretion systems are disclosed. The disclosed type III secretion system inhibitor compounds are useful for combating infections by Gram-negative bacteria such as Salmonella spp., Shigella flexneri, Pseudomonas spp., Yersinia spp., enteropathogenic and enteroinvasive Escherichia coli, and Chlamydia spp. having such type III secretion systems.

The invention belongs to the technical field of environmental protection, and discloses a bacterial type biological adsorbent, and a preparation method and application of the adsorbent. The method comprises the following steps: (1) mixing a bacterial suspension of pseudomonas aeruginosa with a modified liquid containing iron ions and water to obtain a mixed solution; and (2) carrying out a constant-temperature oscillation reaction on the mixed solution, and performing centrifugally washing to obtain the bacterial type biological adsorbent. The preparation method provided by the invention is simple, and has low cost and a short operation period; the prepared adsorbent maintains the original reducing capacity for hexavalent chromium; the adsorption efficiency is obviously increased; the adsorption speed is obviously increased; and the adsorbent has very good removal efficiency for hexavalent chromium.

The present invention provides a method for effective extracellular secretion of a target protein, by preparing a fusion protein connecting LARDS to the target protein and having pI lowered by adjusting the overall charge of target protein, and by using ABC transporter of a bacterial type 1 secretion system (T1SS). The method can allows a protein be produced at a large amount simply and effectively without a separate purification process.

The invention relates to a simple automatic foaming and liquid discharging method for children hand sanitizer. The method comprises the steps of detecting the current emotional state of a child user when the child user is determined to exist in front of a hand sanitizer machine, recognizing whether the child user has the willingness to wash hands or not after the emotional state of the child user is determined, and when the child user is determined to need to wash hands, detecting the bacteria type, the skin type and the pollution degree of the to-be-cleaned palm part of the child user, and enabling the hand sanitizer machine to automatically output the foaming liquid which is matched with the current to-be-cleaned palm skin type of the child user and kills the bacteria type on the palm, so that the hand washing and cleaning requirements of the child user are met. Moreover, the palm skin of the child user is protected, and the damage of non-matched foaming liquid to the palm skin of the child user is avoided.

Apparatuses for sampling mucous from within a sinus to determine if a single sample includes one or more bacterial types indicating bacterial sinusitis comprising an indicator to guide the angle at which a user inserts the apparatus relative to the patient.

A device for sampling mucus from the sinuses to determine whether a single sample includes one or more types of bacteria indicative of bacterial sinusitis, the device including an indicator to guide the user at the angle at which the device is inserted relative to the patient .

The invention relates to a milling tool for steam turbine bacterial-type root blades, and solves the problem in the prior art that no special milling tool for processing the steam turbine bacterial-type blade root blades exists. A positioning block is vertically and fixedly connected with the middle part of one end of an upper end surface of a base plate in the width direction; a support screw passes through a first threaded hole and is in threaded connection with a pressing plate; two sides of the base plate, along the axial direction of a positioning threaded hole, are respectively fixedly connected with an ejection rod support and a support screw sequentially; a screw rod passes through the positioning threaded hole of the positioning block and is connected with a positioning thread; the lower ends of the bacterial-type root blades are clamped on the positioning block for positioning; the screw rod is rotated so as to butt against the bacterial-type root blades; each pressing plate is pressed on the upper surfaces of the bacterial-type root blades; adjusting studs are arranged in a manner of penetrating through guide grooves of the pressing plates; a nut is threadedly connected with the upper end of each adjusting stud so as to press the corresponding pressing plate tightly; and the threaded ejection rods are rotated so as to push two sides of the bacterial-type root blades tightly. The milling tool for the steam turbine bacterial-type root blades, provided by the invention, is used for clamping the steam turbine bacterial-type root blades.

The invention relates to a method for qualitatively and quantitatively detecting bacteria on different wounds based on machine learning, which comprises the following steps of: S1, acquiring a prepared agar plate, and acquiring a plate bacterial colony image; s2, adopting an absdiff difference algorithm of OpenCV for the plate bacterial colony image to obtain a difference result between each subsequent time point of the plate bacterial colony image and the background image, and performing preprocessing to obtain a training set; step S3, constructing and training a Desection Net binary classification detection network; and S4, inputting a plate bacterial colony image of a to-be-detected wound surface sample into the trained classification detection network, and determining different types of bacteria in the sample. The method has accurate qualitative and quantitative ability to culture early bacteria, and the detection efficiency is effectively improved.