The invention discloses a multi-scale imaging method of ultrasonic scatterer diameters based on backscatter coefficients, which is based on ultrasonic radio frequency signals, calculating backscatter coefficients, calculating parameters of ultrasonic scatterer diameters, and calculating multi-scale images of ultrasonic scatterer diameters. Slide the sliding windows of different scales on the ultrasonic radio frequency signal, and calculate the ultrasonic scattering sub-diameter parameters in each sliding window based on the backscattering coefficient, and obtain the ultrasonic scattering sub-diameter parameter value matrix at each scale. The value matrix of the scattering sub-parameters is interpolated to the size of the ultrasonic radio frequency signal, and superimposed and averaged to obtain a multi-scale ultrasonic scattering sub-diameter parameter matrix, and the multi-scale ultrasonic scattering sub-diameter parameter matrix is color-mapped to obtain a multi-scale image of the ultrasonic scattering sub-diameter. The ultrasonic scattering sub-diameter multi-scale imaging method of the present invention can be used for ultrasonic tissue characterization of breast, liver and other biological tissues.