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Mycoplasma culture medium

A culture medium and mycoplasma technology, applied in the direction of microbe-based methods, microbiological determination/inspection, microbiology, etc., can solve the problems of red color, turbidity, false negatives, etc., and achieve reliable detection results

Inactive Publication Date: 2012-07-25
李建新
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many microorganisms that can decompose urea. Some bacteria can turn the color red and cause false positives, and some bacteria and fungi (especially Candida albicans) can make the medium turn red and turbid. The laboratory will generally judge negative, but if At the same time, mycoplasma grows, which will cause false negative judgment due to turbidity
The former domestic commercialized mycoplasma culture medium (including liquid and solid) is generally added with penicillin and polymyxin. Penicillin has a strong effect on most Gram-positive cocci, but has a poor effect on enterococci. Polymyxin is a polypeptide Antibiotics, strong against Gram-negative bacilli such as Pseudomonas aeruginosa, but have no effect on G-cocci and G+ bacteria, neither of which have any inhibitory effect on fungi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 liquid medium, the formula for preparing 1000ml medium contains: peptone 15g; beef heart extract powder 8g; yeast extract powder 5g; phenol red 20mg; sodium chloride 5.2g; L-arginine 0.5g; azoles 30mg; cherimin 2mg; sterile sheep blood 200ml; distilled water added to a total of 1000ml. Adding agar 15g in the same formula can be used to prepare the solid medium of Example 2.

Embodiment 3

[0012] Example 3 liquid medium, the formula for preparing 1000ml medium contains: peptone 15g; beef heart extract powder 8g; yeast extract powder 5g; phenol red 20mg; sodium chloride 5.2g; L-arginine 0.5g; azoles 30mg; custardine 2mg; genipinic acid 2mg; sterile sheep blood 200ml; distilled water added to a total of 1000ml. Adding agar 15g in the same formula can be used to prepare the solid medium of Example 4.

Embodiment 5

[0013] Example 5 liquid medium, the formula for preparing 1000ml medium contains: peptone 15g; beef heart extract powder 8g; yeast extract powder 5g; phenol red 20mg; sodium chloride 5.2g; L-arginine 0.5g; azoles 30mg; cherimoline 2mg; Gongluomu boiled liquid with a concentration of 200g / L 200ml; sterile sheep blood 200ml; distilled water added to a total of 1000ml. Adding 15g of agar in the same formula can be used to prepare the solid medium of Example 6. Example 6 The preparation method of the solid medium is as follows: take gonglao wood, add water and boil for 1 hour, filter, take the filtrate, adjust the volume to a concentration of 200g / L, and obtain the gonglao wood boiled liquid; mix peptone, beef heart extract powder, and yeast extract powder , phenol red, sodium chloride, agar, Gongluomu boiled liquid and distilled water were mixed evenly, shaken on a vortex for 15 minutes, filtered, the filtrate was sterilized at 210°C for 15 minutes, cooled to 45°C, added sterile ...

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PUM

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Abstract

The invention discloses a mycoplasma culture medium and a preparation method thereof. The invention is characterized in that the formula of preparing 1000ml of culture medium contains 15g of peptone, 8g of beef heart infusion, 5g of yeast extract powder, 20mg of phenol red, 5.2g of sodium chloride, 0.5g of L-arginine, 30mg of fluconazole, 2mg of purpureacin, 200ml of sterile sheep blood and the balance of distilled water. Compared with the prior art, the invention has the advantages of reliable detection and high mycoplasma separation rate.

Description

technical field [0001] The invention relates to the field of testing oral microorganism culture, in particular to a culture medium for mycoplasma. Background technique [0002] The proportion of nongonococcal urethritis (NGU) among sexually transmitted diseases (STD) is increasing year by year, ranking first both abroad and domestically, while ureaplasma urealyticum (Uu ) and Mycoplasma hominis (Mh) are the main pathogens of NGU. Therefore, accurate and effective detection of the presence of mycoplasma has become one of the key factors in the diagnosis of NGU. The detection of Uu and Mh is currently mostly carried out by liquid culture method in China. If the medium changes from yellow to red and the liquid is clear, it is positive, and if it does not change color or changes color but the liquid is turbid, it is negative. The principle is that Uu and Mh decompose the urea in the medium to produce alkali to increase the pH, and the color of the phenol red indicator in the m...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/35
Inventor 李建新
Owner 李建新
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