60 results about "Chironomidae" patented technology

The invention relates to the technical field of aquaculture, and specifically relates to a feed for siniperca chuatsi. The feed is prepared by the following raw materials by weight: 55-60 parts of corn, 20-22 parts of rye bran, 10-12 parts of peanuts, 20-25 parts of rapeseed meal, 6-8 parts of chironomidae larvae powder, 8-10 parts of small carp meal, 8-10 parts of loach dry powder, 6-8 parts of herba houttuyniae, 10-12 parts of white fish meal, 6-8 parts of escargot powder, 8-10 parts of dry sphagna powder, 5-6 parts of meat and bone meal, 6-8 parts of radish leaves, 0.5-1 part of rheum officinale, 1-2 parts of saponin, 1-2 parts of felwort, a proper amount of salt and 4-5 parts of a phagostimulant. In consideration of the carnivorous characteristic of the siniperca chuatsi, an abundance of raw materials such as small carp meal, loach dry powder, white fish meal and escargot powder are added in the prepared feed for siniperca chuatsi, so that enough nutrients are provided for the growth and development of the siniperca chuatsi. Traditional Chinese medicine components such as herba houttuyniae, rheum officinale and saponin are added, so that disease-resistant capacity of the siniperca chuatsi can be enhanced. Particularly, parasitic diseases can be prevented; morbidity of the siniperca chuatsi is reduced; and loss of culture benefits can be prevented.

The invention discloses a high-efficiency phagostimulant for colorful angel fish and a preparation method of the phagostimulant. The phagostimulant consists of the following raw materials in percentage by weight: 15-20% of beef heart powder, 15-20% of euphausia superba meal, 15-20% of water flea meal, 15-20% of cutterfish meal, 3-5% of chironomidae larvae powder, 15-20% of compound amino acid, 2-3% of glycine betaine and 3-5% of dimethyl-beta-dimethylpropiothetin; the preparation method comprises the following steps: crushing the beef heart powder, the euphausia superba meal, the water flea meal, the cutterfish meal and the chironomidae larvae powder, screening by using a 80-mesh sieve, putting into a mixing machine, further adding the glycine betaine, the compound amino acid and the dimethyl-beta-dimethylpropiothetin, and uniformly mixing so as to obtain the phagostimulant. By compounding multiple signal phagostimulants, the phagostimulant is good in food calling effect for the colorful angel fish; when the phagostimulant is added into a fish feed, and nutrient components necessary for the growth of the fish can be provided by the amino acid in the phagostimulant, so that the phagostimulant is safe and environmentally-friendly; meanwhile, used raw materials are stable in quality, convenient to purchase and easy to popularize.

The invention provides an ecological-like culture method of glyptosternon maculatum fries. Hatching is performed in parallel tanks, after fries appear for 10 days, microparticle compound feed and chironomidae larvae are adopted for food intake, and the fries are transferred to an ecological-like culture system for culture after being cultured for 20 days and fed with fly maggots. The ecological-like culture system comprises a reservoir, a cement tank, a net cage and a temperature control device, wherein the net cage is placed at the tank bottom of the cement tank, a shading net is set up abovethe net cage, a layer of sand is laid at the bottom of the net cage, and hollow bricks are arranged above the sand; an ultraviolet sterilization device and an aeration device are arranged in the reservoir, a water outlet of the reservoir is connected with a water inlet of the temperature control device, and a water outlet of the temperature control device is connected with a water inlet pipe of the net cage through a pipeline; and a drain pipe is arranged at the bottom of the cement tank. Ecological-like culture of glyptosternon maculatum is truly realized, and the fries have high survival rate and grow well.

The invention relates to preparation of a fermented material in a bait bio-bed for main culturing of macrobrachium nipponensis and auxiliary culturing of Penaeus vannamei Boone and an application method thereof. According to the method, a probiotic-fermented batch is poured into a pond, and a bait bio-bed with the fermented material as the main component is constructed at the bottom of the pond after rotary tillage. The fermented material in the bait bio-bed can directly be eaten by shrimps, and benthonic animals and aquatic plants such as Tubificidae, chironomidae larvae, spiral shell, waterweed and the like can be cultivated in the shrimp habitat to be used as the bait of macrobrachium nipponensis and Penaeus vannamei Boone. Food and habitat completion intensity can be reduced; survival rate and cultivation size of Penaeus vannamei Boone can be raised; and it is beneficial to raise culturing output and benifit of shrimps. To guarantee high propagation capability of demersal food organisms in the bait bio-bed in summer, dual oxygen supply of an oxygen tank and a micropore aerator is adopted. The oxygen tank and the micropore aerator are connected and are started regularly in stages every night in summer.

The invention relates to preparation of a bait bio-bed for breeding of river crabs and an application method thereof, and belongs to the field of a cultivation technology. According to the method, a probiotic-fermented batch is poured into a pond, and a bait bio-bed with the fermented material as the main component is constructed at the bottom of the pond after rotary tillage. The fermented material in the bait bio-bed can directly be eaten by river crabs, and cultivated animals and plants such as Tubificidae, chironomidae larvae, spiral shell, waterweed and the like also can be used as the bait of river crabs. Thus, it is conducive to reduce food competition intensity and raise survival rate of river crabs. By dual oxygen supply of an oxygen tank and a micropore aerator in the bait bio-bed in summer, it is conducive to raise culturing efficiency of demersal food organisms. The cultivated natural bait can be used to provide extra nutrition sources for the growth of river crabs, and it is conducive to raise culturing output and benefit of river crabs.

The invention relates to the technical field of aquaculture and particularly relates to a special loach feed for paddy field cultivation. The feed is prepared from the following raw materials in parts by weight: 20-25 parts of corn meal, 10-12 parts of barley malts, 10-12 parts of buckwheat, 8-10 parts of wheat middling, 10-12 parts of soya bean meal, 6-8 parts of juncus effusus, 4-5 parts of chironomidae larvae powder, 2-3 parts of fish meal, 1-2 parts of mountain flour, 6-8 parts of bagasse, 5-6 parts of albizia julibrissin durazz leaves, 3-4 parts of lotus flower, a suitable amount of table salt and 4-5 parts of phagostimulant. According to the prepared loach feed disclosed by the invention, raw materials, such as chironomidae larvae powder, fish meal, juncus effuses and albizia julibrissin durazz leaves, are properly added so that nutritional components needed by the rapid growth and development of loaches can be met and the digestive absorption of the loaches is accelerated; the utilization rate of the feed is increased and the growth is accelerated so that the cultivation yield is effectively increased.

The invention relates to preparation of a fermented material in a bait bio-bed for main culturing of procambarus clarkii and auxiliary culturing of river crab and an application method thereof. According to the method, a probiotic-fermented batch is poured into a pond, and a bait bio-bed with the fermented material as the main component is constructed at the bottom of the pond after rotary tillage. The fermented material in the bait bio-bed can directly be eaten by shrimps and crabs, and benthonic animals and aquatic plants such as Tubificidae, chironomidae larvae, spiral shell, waterweed and the like also can be cultivated in the shrimp and crab habitat to be used as the bait of procambarus clarkii and river crabs. Thus, food and habitat competition intensity can be reduced, and survival rate of river crabs can be raised. By dual oxygen supply of an oxygen tank and a micropore aerator in the bait bio-bed in summer, it is beneficial to raise culturing efficiency of demersal food organisms. The cultivated natural bait can be used to provide extra nutrition sources for the growth of macrobrachium nipponensis, and it is beneficial to raise culturing output and benefit of macrobrachium nipponensis.

The invention relates to a preparation method of fermentation materials in a bait biological bed for breeding Japanese macrobrachium superbum offspring seed and a use method of the fermentation materials. The method comprises the steps of feeding probiotics-fermented batch mixture into a seedling pool, carrying out rotary tillage, and building a bait biological bed which takes the fermentation materials as the main component at the bottom of the pool, wherein the fermentation materials in the bait biological bed can be directly fed to the Japanese macrobrachium superbum offspring seed, and the benthonic animals such as the tubificidae, the chironomidae larvae, the spiral shell and the aquatic plant as well as the aquatic plant can be cultivated to be taken as the bait of the Japanese macrobrachium superbum offspring seed in a habitat domain, so that the strength of the food competition can be reduced, the seedling survival rate can be improved, and the growth, the seeding yield and the benefit of the Japanese macrobrachium superbum can be improved. In order to guarantee that the benthic bait organisms in the 'bait biological bed' still has high multiplication capacity even in summer, the bait biological bed is aerated by an oxygen bottle and a microporous aerator, and the oxygen bottle and the microporous aerator are connected with each other and are started by stages at the fixed time at every night.

The invention discloses a breeding medium used for a juvenile shrimp and crab pool and a preparation method of the breeding medium.The breeding medium is prepared from, by mass, 10-30 parts of cow dung, 10-30 parts of sheep manure, 10-20 parts of distillers' grains, 5-15 parts of straw, 5-15 parts of corn flour, 5-15 parts of bean pulp, 1-6 parts of molasses, 1-7 parts of vegetable cakes and 2-6 parts of livestock excrement zymophyte.When the breeding medium is used for the breeding processes of freshwater shrimps, eriocheir sinensis and the like, a large number of fresh and good-quality aquatic organisms of water earthworms, chironomidae larvae, plankton and the like can be easily bred, good-quality bait is provided for growth of juvenile crabs, and therefore input of artificial bait is reduced, and the input cost is reduced; meanwhile, by means of the breeding medium, the purified water oxygen dissolving degree is easily increased, the water body environment is adjusted, a good ecological environment is provided for growth of shrimps and crabs, and the survival rate of shrimps and crabs is greatly increased; meanwhile, by means of the breeding medium, the water body fertility can be effectively increased, and growth of aquatic plant root systems is promoted.

The invention discloses a compound feed for feeding giant salamanders. The compound feed is prepared from the following raw materials in parts by weight: 20-25 parts of earthworms, 40-50 parts of water earthworms, 10-16 parts of chironomidae larvas, 12-15 parts of bullfrogs, 15-20 parts of perca fluviatilis, 10-15 parts of breams, 18-22 parts of mackerels, 20-25 parts of rainbow trout, 5-10 parts of mantis shrimps, 10-15 parts of freshwater shrimps, 10-15 parts of krills, 15-20 parts of razor shells, 15-20 parts of field snails, 5-10 parts of blue crabs, 2-5 parts of sea crabs, 15-18 parts of corn flour, 10-20 parts of soybean milk powder, 10-15 parts of fermented peanut meal, 12-15 parts of vitellin, 10-14 parts of whey protein, 5-8 parts of albumin, 3-6 parts of citric acid, 7-10 parts of cod-liver oil, 3-5 parts of compound vitamins, 0.6-1.2 parts of composite minerals, 1-3 parts of a probiotic preparations, 3-4 parts of sodium carboxymethyl cellulose and 2-4 parts of pectin. The invention further discloses a preparation method for the compound feed for feeding the giant salamanders.

The invention discloses a method for artificially breeding giant salamanders, and belongs to aquatic products breeding methods. The method for artificially breeding giant salamanders aims to improve giant salamander population. The method comprises mother giant salamander domesticating, antenatal breeding, parturient breeding, egg laying and hatch breeding and young giant salamander breeding. According to the method, male giant salamanders and female giant salamanders are put into domesticating pools with temperature of about 14 DEG C to be carried out isolation feeding for about three months, the male giant salamanders and the female giant salamanders are put into an identical pool to be fed in the last ten-day of July, live crucian carps or loaches are fed to the giant salamanders every day and water temperature is controlled between 18 DEG C to 22 DEG C for continuous 15 days. After the giant salamanders lay eggs and the eggs are fertilized, live crucian carps or loaches are fed to the giant salamanders every day, the flow speed of pool water is controlled at 0.1 m<3> / s, and after the newborn giant salamanders come out of egg films, the newborn giant salamanders are shifted to a feeding pool with water depth of 5cm. Chironomidae larvae are fed to the newborn giant salamanders once a week after 30 days, and the newborn giant salamanders can grow into young giant salamanders after 6 to 8 months. The method for artificially breeding the giant salamanders can achieve the synchronous growth of the sexual glands of the male giant salamanders and the sexual glands of the female giant salamanders, and improves the success rate of giant salamander breeding and the survival rate of the giant salamander, and is a method of breeding the giant salamanders commercially.

The invention relates to the technical field of aquaculture, in particular to a method for cultivating epinephelus lanceolatus fries. The method comprises the steps that seedling environment conditions are selected; seedling raising preparation work is carried out; egg laying density is arranged; feeding management is carried out; and the water quality is regulated and controlled. In the feeding management, when individuals with the body length exceeding 19 mm are found at the juvenile stage of 18-29 days old, chironomidae larvae are put at the density of 1 piece / ml, firstly, the chironomidae larvae are put at the juvenile stage, and the chironomidae larvae act in water to attract the attention of fries and prey the fries, so that the phenomenon that the same kind of fries are disable is relieved; and then, the juvenile fishes of various body types are physically isolated through screening, and the phenomenon that the juvenile fishes kill one another is further relieved.

The invention discloses a DNA barcode-based identification method for aquatic insect chironomids of the genus Copachironus and the genus oligogonal chironomus. Firstly, the total genome DNA of the insects of the genus Copa and Chironomus oligogonum to be identified was extracted, and used as a PCR template to amplify the sequence of about 650 bp in the front part of the mitochondrial COI using the COI universal primers, and Recover and sequence, and finally identify the species according to the nucleotide characteristic variation sites. The method of the present invention can identify different biotypes and larvae or incomplete individuals of Chironomus genus and Chironomus oligogonum, and has the characteristics of fast, efficient, accurate and convenient. The method has relatively low requirements on the instruments and equipment used, and general It can be carried out in molecular biology laboratories and does not require high professional knowledge of the identification personnel. It is more accurate and reliable than the traditional method of identification relying on the morphological characteristics of adults, which is of great significance.

The invention relates to methods for preparing and applying a fermented feed for a food organism bed for mainly breeding macrobrachium nipponensis and intercropping river crabs. According to the preparing method, a batch mixture which is fermented by using probiotics is fed to a pond, and through rotary tillage, a 'food organism bed' which takes the fermented feed as a main component is established at the bottom of the pond, the fermented feed in the 'food organism bed' can be directly taken by shrimps and crabs, and at the same time, benthonic animals and aquatic plants such as water earthworm, chironomidae larvae, spiral shells and aquatic plants which can be used as a feed for the macrobrachium nipponensis and the river crabs can be cultivated on a habitat area of the shrimps and the crabs, so that the intensity of food competition habitat area competition is alleviated, the survival rate of the intercropped river crabs is increased, and the improvement of the breeding yield and the benefits of the shrimps and the crabs are facilitated. To ensure that a high propagation capability of demersal food organisms in the 'food organism bed' is stilled maintained in summer, oxygen cylinders and micropore aerators are adopted for double-oxygen supply, and the oxygen cylinders are coupled with micropore aeration tubes and are switched on at regular time in different periods each night in summer.

The invention relates to preparation of a fermented material in a bait bio-bed for breeding of procambarus clarkii seedlings and an application method thereof, and belongs to the field of a cultivation technology. According to the method, a probiotic-fermented batch is poured into a seedling growing pond, and a bait bio-bed with the fermented material as the main component is constructed at the bottom of the seedling growing pond after rotary tillage. The fermented material in the bait bio-bed can directly be eaten by procambarus clarkii seedlings, and bred animals and plants such as Tubificidae, chironomidae larvae, spiral shell, waterweed and the like are high-quality baits for shrimp seedlings. Thus, food competition intensity can be reduced, and survival rate of the seedlings can be raised. By dual oxygen supply of an oxygen tank and a micropore aerator in the bait bio-bed in summer, it is conducive to raise culturing efficiency of demersal food organisms. The cultivated natural bait can be used to provide extra nutrition sources for the growth of procambarus clarkii, and it is conducive to raise seedling growing output and benefit of procambarus clarkii procambarus clarkii. In addition, the bait bio-bed is helpful for formation of waterweed communities, seedling growing ecology can be stabilized and seedling growing risk can be reduced.

The invention relates to preparation and application methods of fermented materials of a bait biological bed for mainly cultivating river crabs and intercropping procambarus clarkii and belongs to the technical field of cultivation. The method comprises the following steps: adding materials fermented by probiotics; and carrying out rotary cultivation to construct the bait biological bed which takes the fermented materials as main components at the bottom of a pond. The fermented materials can be directly supplied to the procambarus clarkii to eat; cultured animals and plants including tubificidae, chironomidae larvae, snails, aquatic plants and the like can be used as baits for shrimps and crabs; the abundant natural baits can be used for reducing the competition intensity of foods and inhabit domains; the survival rate of intercropping the procambarus clarkii is improved. The bait biological bed can dually supply oxygen in summer by an oxygen bottle and a micro-pore aerator and is good for improving the cultivation efficiency of demersal food organisms; the cultivated natural baits can be used for providing an extra nutrition source to the growth of the shrimps and the crabs, and are good for improving the cultivation yield and benefits of the shrimps and the crabs; meanwhile, a stable cultivation ecology can be formed by aquatic plant colonies in the pond and the cultivation risk is reduced.

The invention provides preparation and application methods of fermented materials for a bait biological bed for cultivating procambarus clarkii. The method comprises the following steps: adding materials fermented by probiotics; and carrying out rotary cultivation to construct the bait biological bed which takes the fermented materials as main components at the bottom of a pond. The fermented materials not only can be directly supplied to the procambarus clarkii to eat; cultured animals and plants including tubificidae, chironomidae larvae, snails, aquatic plants and the like can be used as natural baits for the procambarus clarkii, so as to be good for reducing the food competition intensity and improving the cultivation survival rate. The bait biological bed can dually supply oxygen in summer by an oxygen bottle and a micro-pore aerator and is good for improving the cultivation efficiency of food organisms; the cultivated natural baits can be used for providing an extra nutrition source to the growth of the procambarus clarkii, and are good for improving the cultivation yield and benefits of the procambarus clarkii.

The invention discloses a Perccottus glenii cage culture technique and management method, and relates to the field of aquaculture. Perccottus glenii is carnivorous fishes and is fed on live baits and mainly fed on larvae of chironomidae, fishes and fish roes in natural world, cannibalization is serious, and when the fish stomachs are dissected, small congeneric fishes often can be found in food. According to the culture technique, expanded feed is put by adopting a cage stocking technique according to the life habits of the Perccottus glenii, feed intake of Perccottus glenii fries is promoted by stocking some fishes liking eating, and the fry growing speed is increased.

The invention discloses a chironomidae intensive feeding and chemical bioactivity measurement device, which comprises a water tank and a top cover, wherein the top cover is arranged above the water tank and is provided with ventilation zones and spawning board plugging slots; a spawning board of a corresponding shape is installed in each spawning board plugging slot; the top end of each spawning board is clamped above the corresponding spawning board plugging slot through a bayonet block.

The invention discloses Chinese sturgeon feed prepared from shrimp meal. The Chinese sturgeon feed comprises, by weight, 50-80 parts of shrimp meal, 10-20 parts of crab, 10-15 parts of clam, 20-30 parts of earthworms, 10-40 parts of chironomidae larvae, 5-20 parts of leech, 15-35 parts of soybeans, 10-20 parts of bean straw, 10-20 parts of duck manure, 10-15 parts of urea, 30-50 parts of blue alga, 15-25 parts of bacillus subtilis, 8.5-15.5 parts of yeast, 6-18 parts of pomace, 10-12 parts of sodium humate, 2-10 parts of table salt, 12-24 parts of alkyl amido betaine, 11-24 parts of bamboo shoots and 11-24 parts of fishbone meal. The Chinese sturgeon feed has the advantages that the Chinese sturgeon feed prepared from the shrimp meal is free of optional side effects on animals if the animals eat the Chinese sturgeon feed for a long term, the immunity of the animals can be enhanced, the shrimp meal contains abundant proteins, the proteins can be easily absorbed by the aquatic animals, and intrinsic delicious and fragrant taste and nutritional components of shrimp can be preserved to the greatest extent.

The invention discloses a feed for promoting gain growth of a young giant salamander. The feed comprises 50-60wt% of a component A and 40-50wt% of a component B, wherein the component A comprises the following raw materials in parts by weight: 5-10 parts of whitebait, 5-10 parts of freshwater shrimp, 10-15 parts of acete chinensis, 5-10 parts of water flea, 15-20 parts of tubificidae, 10-15 parts of chironomidae larvae, 15-20 parts of phryganeid naiad, 10-15 parts of stonefly naiad, 15-20 parts of slug, 5-10 parts of silkworm chrysalis and 20-25 parts of maggot; and the component B comprises the following raw materials in parts by weight: 50-60 parts of fish meal, 5-10 parts of soy protein powder, 30-35 parts of carp meat, 10-15 parts of grass carp meat, 20-25 parts of snail meat, 2-4 parts of deep sea fish oil, 5-7 parts of corn oil, 2-3 parts of vitamin, 2-4 parts of a mineral substance, 3-5 parts of striga asiatica, 5-8 parts of malt, 5-8 parts of fructus amomi, 3-6 parts of gorgon euryale seed, 3-6 parts of endothelium corneum gigeriae galli, 5-8 parts of rhizoma atractylodis macrocephalae, 1-3 parts of quispualis indica, 2-4 parts of aloe and 3-5 parts of wormwood.

The invention discloses a method for extracting DNA of a single chironomidae pupal stage ecdysis, and belongs to the technical field of animal molecular biology. The method comprises the following steps of pretreatment, pyrolysis centrifugation, elution, enrichment, enzymolysis, extraction, centrifugation, precipitation, dissolution, amplification and electrophoresis detection. Compared with the prior art, the method has the beneficial effects that distilled water is used for treatment to remove impurities and prevent interference extraction; under the influence of slow permeation of a 0.9% NaCl solution, the cells remaining on the chironomidae pupal stage ecdysis can gradually recover physiological activity in a mild state, and gradual dissociation of cross-linked protein and DNA in the cells is promoted; and the concentration of the chironomidae pupal stage ecdysis DNA extracted by the method can reach 0.908 ng / [mu]l, and the purity can reach 1.89.

The invention relates to a preparation method of fermentation materials in a bait biological bed for mainly breeding the procambarus clarkia and cooperatively the breeding Chinese softshell turtle and a use method of the fermentation materials, belonging to the technical field of cultivation. The method comprises the steps of feeding probiotics-fermented batch mixture, carrying out rotary tillage, and building a bait biological bed which takes the fermentation materials as the main component at the bottom of the pool, wherein the fermentation materials not only can be directly fed to the procambarus clarkia, but also can cultivate the benthonic food animals such as the tubificidae, the chironomidae larvae, the spiral shell and the shellfish to be taken as the high-quality bait of the procambarus clarkia and the Chinese softshell turtle, so that the survival rate of the cultivated animals can be improved due to the abundant natural bait. The bait biological bed is aerated by an oxygen bottle and a microporous aerator in summer, so that the cultivation efficiency of the benthonic food animals can be improved, and the quick growth of aquatic plant can be promoted, and the formed aquatic plant community can be used for inhabiting the procambarus clarkia and preventing the procambarus clarkia from being attached by the predator. The cooperatively-bred Chinese softshell turtle can prey on the weak procambarus clarkia, so that the disease dispersion can be stopped.

The invention provides a mandarin fish fodder and a preparation method thereof. The mandarin fish fodder is prepared from the following raw materials: cottonseed cake flour, rye bran flour, soybean meal, rape seed cake, chironomidae larvae powder, small carp flour, chicken intestine flour, herba houttuyniae, hemiculter leucisculus flour, escargots flour, yeast powder, meat and bone meal, radish leaf, rheum officinale, saponin, felwort, calcium phosphate, inorganic salt, microecologics and phagostimulant. The preparation method comprises the following steps of (1) after uniformly mixing the cottonseed cake flour, the rye bran flour, the soybean meal and the rape seed cake according to proportion, crushing the mixture into powder; (2) cutting the herba houttuyniae and the radish leaf for later use; (3) after uniformly mixing the rheum officinale, the saponin and the felwort, adding an appropriate amount of water to decoct for 20 to 30 minutes, removing dregs and reserving juice; and (4) mixing the left components except the calcium phosphate and the inorganic salt with the mixtures obtained in the steps (1), (2) and (3), controlling water, adding the calcium phosphate and the inorganic salt, and after uniformly stirring, pelletizing. By the fodder, growth of mandarin fishes can be quickened, and the fodder utilization rate is improved.

The invention relates to a preparation method and a use method of a fermentation material of a bait biological bed for breeding macrobrachium nipponensis. The method comprises the steps of feeding a probiotics-fermented batch mixture, and carrying out rotary tillage for building the 'bait biological bed' which takes the fermentation material as a main component at the bottom of a pond. The fermentation material not only can be directly fed to the macrobrachium nipponensis, but also can be used for cultivating animals and plants such as tubificidae, chironomidae larvae, spiral shell and aquatic weeds, and the cultivated animals and plants can be taken as the bait of the macrobrachium nipponensis, so that food competition intensity can be reduced, and the survival rate of aquaculture can be improved. The 'bait biological bed' is supplied with oxygen by an oxygen bottle and a microporous aerator in summer, so that the cultivation efficiency of the benthonic food organisms can be improved; the cultured natural bait can be used for providing an extra nutrition source for the growth of the macrobrachium nipponensis, so that the aquaculture yield and the benefit of the macrobrachium nipponensis can be increased.

The invention discloses an insecticidal composition for treating parasitic chironomidae, ephydrid larva, blood worm, and rotifer during a spirulina cultivation process. The insecticidal composition iscomposed of sodium bicarbonate, ammonium bicarbonate, calcium chloride, chlorine dioxide, sodium hyposulfite, citric acid, sodium selenite, and fulvic acid according to a mass ratio being 1: 0.1-0.2:0.1-0.15: 0.1-0.15: 0.08-0.1: 0.01-0.03: 0.01-0.03: 0.1-0.2. The insecticidal composition has no toxic and side effect, has good insecticidal effect, cannot remain in cultured objects, and thereby puts an end to food chain pollution. The insecticidal composition can rapidly and efficiently process insect pest, can increase spirulina cultivation capacity by 30%, improves a traditional cultivationmethod, and reduces the high cost of traditional cultivation.

The invention relates to preparation of a fermented material in a bait bio-bed for main culturing of river crab and auxiliary culturing of Litopenaeus Vannamei and an application method, and belongs to the field of a culturing technology. A probiotic fermented material is poured into a pond, and a bait bio-bed containing the fermented material as the main component can be constructed at the bottom of the pond after rotary tillage. The fermented material can directly be eaten by shrimps and crabs, and bred animals and plants such as Tubificidae, chironomidae larvae, spiral shell, waterweed and the like. Thus, it is beneficial to reduce competition intensity for food and territory and raise survival rate and culturing size of Litopenaeus Vannamei. By an oxygen bottle and a micropore aerator for dual oxygen supply of the bait bio-bed, it is beneficial to raise culturing efficiency of demersal food organisms. The cultivated natural bait can be used to provide extra nutrition sources for the growth of shrimps and crabs, and it is beneficial to raise culturing output and benefit of shrimps and crabs.

The invention discloses a method for preparing feed with an effect of improving the immunity of loach seedlings. The method comprises the following steps: mixing beer yeast powder, buckwheat flour, soybean meal, millet flour, sunflower seed meal, coconut meal, rice bran meal, powder of astragalus sinicus, shrimp head meal and powder of chironomidae larvae, grinding the mixture, adding corncob powder, green tea dust, yttrium tartrate hemihydrate and edible salt into the mixture, and performing uniform mixing to obtain a first material; stirring cotton fibers, corncob powder, cationic guar gum and water, adjusting the moisture content, performing grinding, adding grapeseed oil into the mixture, performing heating and stirring, and performing drying and grinding to obtain a second material; and adding water into the first material, performing steaming and cooling, adding astragalus polysaccharides, mountain flour, calcium hydrophosphate, valine, leucine and trace elements, performing uniform mixing, performing granulating and enveloping, and spraying the second material into the mixture in the granulating and enveloping process to obtain the feed with the effect of improving the immunity of loach seedlings.

The invention discloses a toxicological experiment device and a method for evaluating pesticide toxicity by using the toxicological experiment device. The toxicological experiment device comprises an experiment bin, a controller, a water inlet pipe and a water outlet pipe, a plurality of beakers are arranged in the experiment bin, and an automatic electronic valve is arranged on each branch pipeline for communicating the water inlet pipe with one beaker; a drain hole with a filter screen is formed in the side wall of the lower edge of the opening end of each beaker; the drain hole of each beaker is communicated through a branch pipeline water pipe 104; an automatic electric heating device is arranged at the bottom of the experiment bin; and a temperature sensor is also arranged in the experiment cabin. According to the pesticide toxicity evaluation method, the toxicity experiment device is used as main experiment equipment, chironomidae larvae are used as experiment objects, and the fatality rates of the Bt proteins with different concentrations on the chironomidae larvae are used as main indexes to evaluate the pesticide toxicity. The device is simple in structure, and the reliability of the pesticide toxicity evaluation method is high.

The invention aims to provide an octopus variabilis larva overwintering-culture method for overwintering culture of octopus variabilis larvae. In a culture process, bait for feeding refers to chironomidae larvae, an aquaculture density of the octopus variabilis larvae is 300-400 larvae / m<2>, and an aquaculture temperature is controlled in a range of 13-18 DEG C. During aquaculture, chilled chironomidae larvae are adopted for bait conversion of the octopus variabilis larvae, and after bait conversion, the chilled chironomidae larvae are used for overwintering culture. The bait for bait conversion has advantages of high nutritive value, low cost, easiness in acquisition, freeness of external environmental limitations and the like. During overwintering culture, the survival rate and the growth rate in aquaculture of the octopus variabilis larvae are increased under the condition of shortage of living baits, and large-scale artificial generation breeding of octopus variabilis is supportedstrongly.