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DNA (deoxyribonucleic acid)-bar-code-based identification method of aquatic insects in Chironomidae two genera

An aquatic insect, barcode technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as difficulty in morphological identification of Chironomidae and Chironomyces oligohorn, save time and cost, shorten time The effect of low cycle and professional knowledge requirements

Inactive Publication Date: 2016-06-29
HUAZHONG AGRI UNIV
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Problems solved by technology

[0006] The object of the present invention is to provide a DNA barcode-based identification method for aquatic insects Chironomus genus Copachironus and Chironomus oligogonum, which solves the problem of difficult morphological identification of Chironomus genus and Chironomus oligogonum, The method is scientific, effective, fast and accurate

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  • DNA (deoxyribonucleic acid)-bar-code-based identification method of aquatic insects in Chironomidae two genera
  • DNA (deoxyribonucleic acid)-bar-code-based identification method of aquatic insects in Chironomidae two genera
  • DNA (deoxyribonucleic acid)-bar-code-based identification method of aquatic insects in Chironomidae two genera

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Embodiment 1

[0020] The present invention will be further described below in conjunction with specific embodiments:

[0021] A DNA barcode-based identification method for aquatic insects Chironomidae and Chironomus oligohorne includes the following steps:

[0022] (1) Sample collection, identification and storage: use Sauber net (area 0.3m×0.3m, aperture 0.5mm) and 1 / 16m 2 The Peterson mud picker was used to collect samples from the Yili River and the Irtysh River in Xinjiang. The collected samples were cleaned through a 60-mesh sieve and poured into a white porcelain dish to select the genus Pachironomis and Oligocera for the experiment. Mosquitoes are insects, and samples are preserved with absolute ethanol. Keep replacing the absolute ethanol (before the solution turns yellow), use a portable refrigerator to bring it back to the laboratory for analysis, and distinguish the types according to the Lowe's apparatus of the sample under the microscope. The Lowe's apparatus of the genus Oligochiro...

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Abstract

The invention discloses a DNA barcode-based identification method for aquatic insect chironomids of the genus Copachironus and the genus oligogonal chironomus. Firstly, the total genome DNA of the insects of the genus Copa and Chironomus oligogonum to be identified was extracted, and used as a PCR template to amplify the sequence of about 650 bp in the front part of the mitochondrial COI using the COI universal primers, and Recover and sequence, and finally identify the species according to the nucleotide characteristic variation sites. The method of the present invention can identify different biotypes and larvae or incomplete individuals of Chironomus genus and Chironomus oligogonum, and has the characteristics of fast, efficient, accurate and convenient. The method has relatively low requirements on the instruments and equipment used, and general It can be carried out in molecular biology laboratories and does not require high professional knowledge of the identification personnel. It is more accurate and reliable than the traditional method of identification relying on the morphological characteristics of adults, which is of great significance.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for identifying two genera of aquatic insects Chironomidae based on DNA barcodes, which can quickly identify insects of the genus Chironomidae and the genus Chironomidae. Background technique [0002] Aquatic insects are one of the most important biological groups in aquatic ecosystems. As an important basic bait resource for fish, aquatic insects play an important role and function in the process of material circulation and energy flow in water bodies. In addition, aquatic insects play an important role in water quality evaluation. In recent years, water environment assessment based on various biological indexes and diversity indexes of aquatic insects has been widely used at home and abroad. The prerequisite is the accurate classification and identification of aquatic insects. Traditional classification methods are mainly based on morphology to identify species. Th...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 周琼郑圆圆谢从新张秀杰王军葛奕豪
Owner HUAZHONG AGRI UNIV
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