A Rapid Identification Method of Lucilia sericata

Abstract

The invention belongs to the technical field of molecular biological identification methods, in particular to a method for quickly identifying Lucilia sericata, which includes collecting fly stumps and extracting DNA samples, designing specific primers, and performing PCR amplification on samples to be identified. The product was electrophoresed on agarose gel, and whether the sample was Lucilia sericata was judged according to the presence or absence of fragment bands. The present invention designs specific primers for Lucilia sericata, uses rapid molecular identification SS-PCR technology to quickly and accurately identify it, and can identify larvae, eggs or pupae, and even residual bodies such as heads, chests, wings, and feet Identification greatly overcomes the difficulty of morphological identification and shortens the period of forensic identification.

Description

Technical field [0001] The present invention belongs to the technical field of molecular biological identification, and more particularly to a rapid identification of a filamentous green flies. Background technique [0002] In the practice of forensic on-site work, the PostMortem Interval (PMI) is one of the important tasks of autopsy. Correctly estimate that PMI is the key to criminal case detection. The variation of benesium fly with death is present in different growth and developmental regulations, which is of great significance for the death time PMI. The flyn destination is first discovered and reached the body, and the eggs were produced on the body, so according to the developmental state of the bodies, the pmi can be inferred. [0003] However, deaths often have different benestrophic phres, eggs, or cockroaches, even for head, chest, wings, foot and other degeneration, and accurate identification of gerbera of gerberans. For the classification of larvae, it is much more...

AI Technical Summary

Problems solved by technology

[0003] However, there are often larvae, eggs or pupae of different sarcophilus flies at the death scene, and even head, thorax, wings, feet and other remnants, which make it difficult to accurately identify the species of sarcophilus flies

The classification and identification of larvae is much more difficult than that of adults. Usually, larvae are reared indoors and then identified as adults, which takes a long time, and morphological identification requires rich experience. There are only a few morphological species in my country. Experts have the ability to

In view of the diversity and complexity of flies at the crime scene, it is difficult for non-insect professionals to identify their morphological species, so it is urgent to develop technologies for the rapid identification and identification of different sarcophilus flies

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