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Method for authenticating salt tolerance of cotton seeds

A technology for cotton seeds and salt tolerance, applied in the field of seed screening and evaluation, can solve the problems of difficult control of the identification technology environment, long identification period, large human error, etc., and achieve the effects of short identification period, easy promotion and implementation, and easy operation.

Inactive Publication Date: 2019-02-01
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for identifying the salt tolerance of cotton seeds, which aims to solve the shortcomings of traditional identification technology that the environment is not easy to control, the identification cycle is long, the efficiency is low, and the human error is large

Method used

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  • Method for authenticating salt tolerance of cotton seeds
  • Method for authenticating salt tolerance of cotton seeds
  • Method for authenticating salt tolerance of cotton seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Step 1. Sample preparation:

[0040] From the fully mixed net seeds, 2000 9807 cotton seeds were randomly selected.

[0041] Step 2, Stress cultivation: Set up four concentration gradients of 0.5%, 1.0%, 1.5% and 2.0%, and repeat each concentration 4 times, with 100 grains per repetition. Add 15 ml of 0.5%, 1.0% and 1.5% sodium chloride solution to every 100 g of dry sand, the pH is 6.0-7.5, stir evenly, spread 1.5 cm thick wet sand in the germination box, and put 4 repeated seeds to be tested , placed evenly on the flattened sand bed, flattened the seeds with a flat-bottomed container, making half of them sink into the sand, and covered with a layer of wet sand with a thickness of 1.0 cm, spread evenly, covered, and placed at 30 ℃ In the light incubator at ±1 °C, after the cotyledon was exposed to the sand surface, it was lighted for 8 hours during the day, and the light intensity was ≥1200 lx.

[0042] Step 3, control cultivation: 4 repetitions, 100 grains per repet...

Embodiment 2

[0045] Step 1. Sample preparation:

[0046] From the fully mixed net seeds, 2000 grains of 79 cotton seeds of China Cotton Institute.

[0047] Step 2, Stress cultivation: Set up four concentration gradients of 0.5%, 1.0%, 1.5% and 2.0%, and repeat each concentration 4 times, with 100 grains per repetition. Add 15 ml of 0.5%, 1.0% and 1.5% sodium chloride solution to every 100 g of dry sand, the pH is 6.0-7.5, stir evenly, spread 1.5 cm thick wet sand in the germination box, and put 4 repeated seeds to be tested , placed evenly on the flattened sand bed, flattened the seeds with a flat-bottomed container, making half of them sink into the sand, and covered with a layer of wet sand with a thickness of 1.0 cm, spread evenly, covered, and placed at 30 ℃ In a light incubator at ±1 °C, light for 8 h during the day was started after the cotyledons were exposed to the sand surface.

[0048] Step 3, control cultivation: 4 repetitions, 100 grains per repetition. Add 15 ml of deionize...

Embodiment 3

[0051] Step 1. Sample preparation:

[0052] From the fully mixed net seeds, randomly select 2000 J7514 cotton seeds.

[0053] Step 2, Stress cultivation: Set up four concentration gradients of 0.5%, 1.0%, 1.5% and 2.0%, and repeat each concentration 4 times, with 100 grains per repetition. Add 15 ml of 0.5%, 1.0% and 1.5% sodium chloride solution to every 100 g of dry sand, the pH is 6.0-7.5, stir evenly, spread 1.5 cm thick wet sand in the germination box, and put 4 repeated seeds to be tested , placed evenly on the flattened sand bed, flattened the seeds with a flat-bottomed container, making half of them sink into the sand, and covered with a layer of wet sand with a thickness of 1.0 cm, spread evenly, covered, and placed at 30 ℃ In a light incubator at ±1 °C, light for 8 h during the day was started after the cotyledons were exposed to the sand surface.

[0054] Step 3, control cultivation: 4 repetitions, 100 grains per repetition. Add 15 ml of deionized water to every 10...

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Abstract

The invention belongs to the technical field of seed screening and assessment in the field of agriculture and discloses a method for authenticating salt tolerance of cotton seeds. The method includes:randomly counting full cotton seeds from completely-mixed pure seeds, dividing the seeds into a sodium chloride treatment group and a contrast treatment group, cultivating in an illumination incubator for preset time, researching germinating seed amount of each treatment group, calculating salt tolerance coefficients, and authenticating and assessing salt tolerance of the cotton seeds through analytic comparison of the salt tolerance coefficients. The method is low in cost, short in period, easy to operate, convenient to popularize and implement everywhere and especially suitable for large-scale genetic resource salt tolerance screening.

Description

technical field [0001] The invention relates to a method for identifying seeds, in particular to a method for identifying the salt tolerance of cotton seeds, and belongs to the technical field of seed screening and evaluation in the field of agriculture. Background technique [0002] Soil salinization is a worldwide problem. According to statistics, the global saline-alkali land area currently exceeds 800 million hectares, accounting for 6% of the total land area. my country's existing saline-alkali land is nearly 27 million hectares, accounting for about 25% of the arable land area, ranking fourth in the world after Australia, Mexico, and Argentina. Due to the existence of a large amount of saline-alkali soil, it is difficult to use a large amount of cultivated land, which restricts the sustainable development of agriculture and poses a great threat to agricultural production safety. [0003] my country is one of the countries with the largest cotton planting scale in sal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 王延琴陆许可王俊娟金云倩匡猛马磊周大云方丹徐双娇王爽荣梦杰蔡忠民黄龙雨吴玉珍
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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