A method for identifying the resistance of cotton seeds to salt stress

Abstract

The invention provides a method for identifying salt-stressed resistance in cotton seeds, comprising: selecting full, neat and intact cotton seeds from different varieties of seed, sterilizing the seeds, dividing the cotton seeds into 9 salt-stressed concentration groups, transplanting respectively to aeroponic boxes, each holding a culture solution which is salt-stressed solution of corresponding concentration, controlling ultrasonic atomizers in the aeroponic boxes to operate for 1-3 min in intervals of 5-10 min by using a timing switch, and changing the culture solution in the aeroponic boxes every 20-25 hours; over a preset time, making statistics for sprouting condition of each salt-stressed concentration group, and screening more salt-tolerant varieties by analysis and comparison of sprouting condition data. The method is low in cost, easy to perform and easy to popularize and implement in various places.

Description

technical field [0001] The invention relates to a method for identifying seeds, in particular to a method for identifying the resistance of cotton seeds to salt stress. Background technique [0002] Salt stress mainly refers to the high osmotic potential effect of high salinity on plant growth and development. [0003] For a long time, the screening or identification of plant salt stress has been carried out in experimental fields for stress experiments, or some physiological and biochemical parameters have been counted indoors as a reference for comparison. This method is directly identified in the field, and the identification results are relatively straightforward, but cotton only has a specific growing season each year, that is, only a specific batch of tests can be carried out each year; in addition, the requirements for the identification site are relatively high, such as salt ponds or saline-alkali land for salt stress experiments The site cannot be changed, because ...

AI Technical Summary

Problems solved by technology

This method is directly identified in the field, and the identification results are relatively straightforward, but cotton only has a specific growing season each year, that is, only a specific batch of tests can be carried out each year; in addition, the requirements for the identification site are relatively high, such as salt ponds or saline-alkali land for salt stress experiments The site cannot be changed, because the saline-alkali land or salt pool that has passed this type of identification test cannot be used to plant other plants to prevent salt damage to other crops, which occupies a large area and the identification efficiency is low

Moreover, the impact of salt stress on cotton is in a qualitative range, and there is no specific numerical quantification. The results observed by different inspectors are quite different, and the conclusions drawn are different and unstable. The comparability of the test results between the years and between parallels poor

[0004] At present, there are many methods for identifying the resistance of cotton seeds to salt stress, but all of them are cumbersome and have a long period of time, and the detection accuracy is not high.

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