The invention discloses anti-fatigue soybean milk and a preparation method of the soybean milk. The soybean milk is prepared from the following raw materials: soybean meal, green gram starch, sweet potato flour, stigmata maydis, toona sinensis leaf, ginseng, paniculate microcos leaf, rainbow conk, astragalus mongholicus, codonopsis pilosula, cimicifugae foetidae, gorgon euryale seed, Chinese yam, crinite pogonatherum herb, adhesive rehmannia leaf, sunflower seed, pomegranate seed, arabidopsis thaliana, dragon's tongue leaf, royal jelly, sweet potato leaf, spinach and appropriate amount of pure water. The preparation method disclosed by the invention is simple; the prepared anti-fatigue soybean milk uses the soybean meal, the green gram starch and the sweet potato flour as the raw materials, and is rich in nutrient, low in fat, and high in plant protein; a plurality of anti-fatigue Chinese medicines such as the astragalus mongholicus, the codonopsis pilosula, the cimicifugae foetidae, the gorgon euryale seed and the Chinese yam and anti-fatigue vegetables such as the spinach are added into the soybean milk; and the soybean milk is refreshing, delicious, and fragment in smell and has the effects of refreshing oneself and resisting fatigue.