Method for identifying bacterial wilt resistance of tobacco seedlings
A tobacco bacterial wilt and seedling stage technology, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of large influence of seasons, short identification period, long identification period, etc. Strong stability, short identification cycle and stable identification results
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[0016] 1. Materials and Methods
[0017] 1.1 Test material
[0018] Identification of bacterial wilt resistance in 12 tobacco cultivars. D101, Long Neck Yellow, Big Tiger Ear, Ti448A, Honghua Dajinyuan, K326, Nanjiang No. 3, Yunyan 85, Yanyan 97, K346, RG17, China Tobacco 100
[0019] 1.2 Test method
[0020] Mix the water-retaining agent and perlite at a ratio of 2:1, put them into a 6-well cell culture plate after disinfection, and sow tobacco seeds on the cell culture plate. Sow the 12 tested varieties into one hole respectively, 10-20 seeds / hole. The experiment set up 4 repetitions. The seeded cell culture plate was cultured in a sterile culture room at a temperature of 25°C, 16 hours of light per day, a light intensity of 4000 lux (lux), and a relative temperature of 70-80%. After emergence, 5 seedlings were uniformly reserved in each hole. When the seedlings grow to two true leaves (25-30 days), inoculate the suspension of R. solanacearum into each well of the cel...
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