Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for identifying bacterial wilt resistance of tobacco seedlings

A tobacco bacterial wilt and seedling stage technology, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of large influence of seasons, short identification period, long identification period, etc. Strong stability, short identification cycle and stable identification results

Active Publication Date: 2014-11-19
GUIZHOU TOBACCO SCI RES INST
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: Aiming at the problems in the prior art that the resistance identification is unstable, the identification period is long, and it is greatly affected by soil fertility and seasons, the present invention provides a method for identifying tobacco bacterial wilt resistance at the seedling stage. It is a unique method, which has the advantages of short identification cycle, no seasonal limit and stable identification results.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Materials and Methods

[0017] 1.1 Test material

[0018] Identification of bacterial wilt resistance in 12 tobacco cultivars. D101, Long Neck Yellow, Big Tiger Ear, Ti448A, Honghua Dajinyuan, K326, Nanjiang No. 3, Yunyan 85, Yanyan 97, K346, RG17, China Tobacco 100

[0019] 1.2 Test method

[0020] Mix the water-retaining agent and perlite at a ratio of 2:1, put them into a 6-well cell culture plate after disinfection, and sow tobacco seeds on the cell culture plate. Sow the 12 tested varieties into one hole respectively, 10-20 seeds / hole. The experiment set up 4 repetitions. The seeded cell culture plate was cultured in a sterile culture room at a temperature of 25°C, 16 hours of light per day, a light intensity of 4000 lux (lux), and a relative temperature of 70-80%. After emergence, 5 seedlings were uniformly reserved in each hole. When the seedlings grow to two true leaves (25-30 days), inoculate the suspension of R. solanacearum into each well of the cel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying the bacterial wilt resistance of tobacco seedlings. A water retaining agent and pearl stone are mixed uniformly at the ratio of 2:1; the mixture is disinfected and then loaded into a 6-hole cell culture board; tobacco seeds are sowed on the cell culture board, wherein each kind of tobacco seeds is sowed in one hole, and 10-20 tobacco seeds are sowed in each hole; the cell culture board is placed in an axenic culture room to culture the tobacco seeds under the conditions that the temperature environment is 25 DEG C, the illumination time is 16 hours every day, the illumination intensity is 4,000 lux and the relative humidity is 70-80 %; after emergence of seedlings, 5 seedlings are reserved in each hole uniformly; a nutrient solution is prepared from special tobacco seedling raising fertilizers, wherein the concentration of a nitrogen fertilizer reaches 100 ppm; the nutrient solution is top-dressed into the culture board; when the seedlings send forth two true leaves, 1 ml of ralstonia solanacearum suspending liquid is vaccinated into each hole of the cell culture board through a liquid-transferring gun; investigation and recordation are carried out 15 days later. The method provided by the invention is shorter in identification period, can identify 25-day-old seedlings, is not limited by seasons and has high testing stability.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, in particular to a method for identifying tobacco bacterial wilt resistance at the seedling stage. Background technique [0002] A tobacco disease caused by bacteria has occurred in many smoking areas in my country and is seriously harmful. There is no specific control agent for bacterial wilt, and once it occurs, it is difficult to control it. In areas prone to bacterial wilt, the most important method is to use tobacco varieties resistant to bacterial wilt. The identification of bacterial wilt disease resistance of tobacco varieties mainly relies on the identification of field disease nurseries, but the identification of field disease nurseries has the disadvantages of long identification time, high cost, great influence of soil fertility and season, and unstable resistance identification. Chinese patent CN 200910094174 "A Seedling Identification Method for Tob...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18A01G7/00
Inventor 王仁刚任学良林世锋谢升东王轶
Owner GUIZHOU TOBACCO SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products