37results about How to "Identification results are stable" patented technology

The invention relates to a method for identifying an aphid-resistant transgenic cotton. The method comprises the following steps: planting control varieties and checked varieties in a flower pot with a cover at room temperature; carrying out artificial aphid inoculation on each control variety and each checked variety; investigating the number of cotton aphids on each plant of the cotton the third day after the artificial aphid inoculation is carried out; and taking the blade of the cotton plate most severely damaged as a standard leaf to investigate aphid harm magnitude of the control varieties and the check varieties the seventh day after the artificial aphid inoculation is carried out, and identifying the transgenic cotton variety resistant to the aphids according to the aphid hazard index and aphid hazard index deceasing rate. The method provided by the invention features good practicability and operability and stable and reliable identification result.

The invention provides a specific primer for identifying donkey-sourced components in colla corii asini, a kit and an identifying method. A large quantity of primers are designed on the basis of CYT Band COX-1 genes of donkey; through screening and research on the primers, a primer with species specificity is screened; the primer with species specificity can be used for identifying donkey-sourcedcomponents in colla corii asini and accurately separating donkey from other adulterants; a PCR amplification product thereof is only 70-120 bp and molecular weight is small; identification for a large quantity of donkey-sourced components in colla corii asini in a short time can be guaranteed; an identification result is accurate, stable, reliable and highly sensitive.

The invention discloses a molecular marking primer and method for identifying Chinese coastal platycephalus indicus species indeterminate (sp) and platycephalus cultellatus. The sequence of the molecular marking primer Fish F1 is 5'-TCAACCAACCACAAAGACATTGGCAC-3', and the sequence of the molecular marking primer Fish R2 is 5'-ACTTCAGGGTGACCGAAGAATCAGAA-3'. The molecular marking method includes: extracting DNA of platycephalus indicus species indeterminate (sp) and platycephalus cultellatus, and taking the DNA of the two fish as templates for PCR amplification, performing purification and sequencing to the amplification results, and comparing the sequencing results. The molecular marking primer is proper in length, is closely complementary to the sequences of the templates, and is beneficialfor an amplification reaction. The molecular marking method is simple, the identification result is stable, and the repeatability is good. The amplification result of Chinese coastal platycephalus indicus species indeterminate (sp) is consistent, so that the primer and method show high consistency.

The invention belongs to the technical field of maize aspergillus flavus ear rot resistance, and discloses an indoor rapid identification method for maize disease resistance, wherein the method includes the steps: A, preparing an aspergillus flavus spore suspension; B, preparing samples of sterilized test maize varieties; C, inoculating each grain of each sterilized maize sample with the aspergillus flavus spore suspension, and culturing at constant temperature to obtain maize varieties infected with disease; and D, taking the disease-infected variety with the disease grade of 9 as control, determining the average disease grade of the maize varieties infected with the disease, and classifying the resistance according to the average disease grade, so as to identify the maize aspergillus flavus ear rot resistance. The method has the advantages of short identification period, low identification cost and accurate results.

The invention provides a molecular marker for identifying a common threewingnut root medicinal material, a primer pair, a PCR reagent, a kit and application of the molecular marker in identification of the common threewingnut root medicinal material. The molecular marker is in a nucleotide sequence shown in SEQ ID NO.3, and the primer pair can amplify the nucleotide sequence shown in SEQ ID NO.3. The invention further provides an identification method of the common threewingnut root medicinal material. Through the molecular marker, the primer pair, the PCR reagent, the PCR kit and the identification method, whether or not the molecular marker is amplified is detected so that the common threewingnut root medicinal material can be identified, the repeatability is high, and the defects of high subjectivity and poor repeatability of a traditional identification method are overcome.

The invention provides a specific primer, a probe, a kit and a method for detecting barley component. A large number of primers are designed based on FDH, GLU2a, RBCL, ACPS and UDPg genes of barley, and primers and probes with species specificity are screened out through a large number of primer screening researches. When the primers and the probes are used for detecting barley component in a sample to be detected, barley and other species adulterants can be accurately separated, the absolute detection sensitivity reaches 0.2 copies/microliter, the relative sensitivity can reach 0.5%, and theidentification result is accurate, stable and reliable.

The invention provides a method for identifying a fertilization mode of an ictalurus punctatus culture pond, which comprises the following steps of: extracting total DNA (deoxyribonucleic acid) of intestinal microorganisms of the ictalurus punctatus in the culture pond, designing a primer aiming at a DNA sequence of 16S rDNA (ribosomal deoxyribonucleic acid) gene of a Lactobacillus population by taking the total DNA as a template, performing PCR (polymerase chain reaction) amplification, and judging that inorganic fertilizer is used in the culture pond if an amplification product does not have a 1054bp band. According to the method for identifying the fertilization mode of the ictalurus punctatus culture pond, the channel catfish pond fertilization mode can be rapidly, simply, accurately and effectively identified, the operation is simple, the cost is low, the accuracy is high, the identification result is stable and reliable, and the problem that the channel catfish fertilization mode is difficult to identify can be solved.

The invention provides a specific primer, a probe, a test kit and a method for detecting sugarcane components. According to the invention, a large number of primers are designed on the basis of the ITS gene, rbcl gene, nsLTP gene and NDR1/HIN1 gene of sugarcane; meanwhile, through screening research on the large number of primers, a primer and a probe with species specificity are screened out; andthe above-mentioned primer and probe can be used for detecting the sugarcane components in a to-be-detected sample, can accurately separate sugarcane from species, can reach an absolute detection sensitivity of 0.4 copies/[mu]L and a relative detection sensitivity of 0.01%, and have accurate, stable and reliable identification results.