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Edible sunflower hybrid SH363 authenticity rapid detection kit

A technology of SH363 and sunflower, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of no evaluation standard, long time consumption, and easy to be affected by climate and cultivation conditions, etc., and achieve stable identification results and detection The effect of accurate and efficient variety identification

Active Publication Date: 2016-05-11
三瑞农业科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are following defects in above-mentioned identification method: (1) be limited by experience, do not have completely consistent judging standard; (2) identification in sunflower growth period is also easily affected by climate and cultivation condition, causes error or misjudgment; (3) ) need to invest a lot of manpower and material resources, high cost, time-consuming, poor timeliness, the guidance of the market and production is not timely, it is difficult to avoid the loss of production
The variety has clean foliage at maturity and is resistant to downy mildew and Verticillium wilt; very low incidence of Sclerotinia

Method used

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  • Edible sunflower hybrid SH363 authenticity rapid detection kit
  • Edible sunflower hybrid SH363 authenticity rapid detection kit
  • Edible sunflower hybrid SH363 authenticity rapid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH363 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0058] The primer in the primer liquid part is primer SR-366, and the SR-366 primer is:

[0059] SR-366-F: 5'-AACCAACTGAGCATTCTTGTGA-3',

[0060] SR-366-R: 5'-GCGCTAGGTTAAAGAGGACAAA-3'.

[0061] The formula of each described kit is:

[0062] Primer solution, 0.2μM, 1μL;

[0063] TaqDNA polymerase, 2.5units, 0.5μL;

[0064] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0065] dNTPs, 2.5mM, 2μL;

[0066] wxya 2 O, 17 μL.

[0067] The method for rapidly detecting the authenticity of the edible sunflower hybrid SH363 by using the above kit comprises the following steps:

[0068] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by the edible sunflower hybrid SH363 and its parent standard sunflower seeds A436 ...

Embodiment 2

[0091] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH363 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0092] The primer in the primer liquid part is primer SR-495, and the SR-495 primer:

[0093] SR-495-F: 5'-CCAGGATTAGGTAGCTTAGTTCG-3',

[0094] SR-495-R: 5'-GCGATCTGAGGTTGACTCGT-3'.

[0095] The formula of each described kit is:

[0096] Primer solution, 0.2μM, 1μL;

[0097] TaqDNA polymerase, 2.5units, 0.5μL;

[0098] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0099] dNTPs, 2.5mM, 2μL;

[0100] wxya 2 O, 17 μL.

[0101] The method for rapidly detecting the authenticity of the edible sunflower hybrid SH363 by using the above kit comprises the following steps:

[0102] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by the edible sunflower hybrid SH363 and its parent standard sunflower seeds A436 and ...

Embodiment 3

[0125] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH363 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0126] The primer in the primer solution part is primer SR-1067, and the SR-1067 primer:

[0127] SR-1067-F: 5'-CGTCAACTAATGCTGTCCTGATG-3',

[0128] SR-1067-R: 5'-TCGGATATGATGCTGCTAAAGGT-3'.

[0129] The formula of each described kit is:

[0130] Primer solution, 0.2μM, 1μL;

[0131] TaqDNA polymerase, 2.5units, 0.5μL;

[0132] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0133] dNTPs, 2.5mM, 2μL;

[0134] wxya 2 O, 17 μL.

[0135] The method for quickly identifying the authenticity of the edible sunflower hybrid SH363 using the kit comprises the following steps:

[0136] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by the edible sunflower hybrid SH363 and its parent standard sunflower seeds A436 and ...

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Abstract

The present invention provides an edible sunflower hybrid SH363 authenticity rapid detection kit which includes a primer liquid portion, a reaction liquid portion and a DNA polymerase portion which are each individually wrapped, and the primer liquid portion comprises the at least one primer of primer SR-366, primer SR-495, primer SR-1067 and primer SR-1079. The kit can detect a large number of to-be-tested sunflowers in a short time, and the detection results are accurate, stable and reliable.

Description

technical field [0001] The invention belongs to the field of identifying the authenticity and variety purity of crop seeds, and in particular relates to a kit for quickly detecting the authenticity of sunflower hybrid SH363 established based on SSR molecular marker technology. Background technique [0002] Sunflower (Helianthus annuus L.) is a dicotyledonous plant of the genus Helianthus in the Compositae family (Composicae). It is characterized by strong resistance, rich oil content, and high linoleic acid content in oil. It is the second largest oil crop in the world. There are many varieties of sunflower, among which edible sunflower has high content of unsaturated fatty acids, especially linoleic acid and α-wheat germol (a kind of vitamin E), which is not only rich in nutrition, but also effective in preventing and treating cardiovascular diseases. People love it. Edible sunflowers are plentiful, with hundreds of landraces roaming the country. With the continuous deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 司立平张永平马德甯万县贞姚梅园
Owner 三瑞农业科技股份有限公司
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