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Exopalaemon carinicauda sex-linked marker and sex test method

A technology for the white shrimp and detection method, which is applied in the fields of biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc., can solve the problems such as sex-linked molecular markers of white shrimp that have not been seen yet, and achieve stable identification results , easy operation and low sample requirements

Active Publication Date: 2019-03-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

So far, there is no report of sex-linked molecular markers in white shrimp

Method used

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  • Exopalaemon carinicauda sex-linked marker and sex test method
  • Exopalaemon carinicauda sex-linked marker and sex test method

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Embodiment 1

[0021] 1) DNA extraction

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Abstract

The invention provides an exopalaemon carinicauda sex-linked marker and a sex test method. The process of the test method is as follows: the muscular tissue genome DNA of exopalaemon carinicauda is extracted, and with the extracted genome DNA as a template, PCR (Polymerase Chain Reaction) amplification reaction is carried out; the PCR amplification product is purified and sequenced; the sequencingresult is compared with the DNA sequence of the exopalaemon carinicauda sex-linked marker, the sex is female if the sequencing map is shown as miscellaneous peaks, and the sex is male if the sequencing map is a single peak and is consistent with the marker sequence. The test method disclosed by the invention is easy to operate, the requirement on samples is relatively low, and identification results are stable, and are not influenced by tissue specificity and environment. The invention can conveniently, efficiently and relatively accurately identify the genetic sexes of exopalaemon carinicauda. The invention has a good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal biology, and in particular relates to a sex-linked marker and a sex detection method for white prawns. Background technique [0002] Chicktail White Shrimp ( Exopalaemon carinicauda ), also known as white shrimp, five-bearded shrimp, and winter prawn, belongs to Crustacea, Decapod, Swimming suborder, Longarm Shrimpidae, and White Shrimp. It is distributed in shallow seas and low-salt waters along the coast of mainland China and the west coast of the Korean Peninsula. The Bohai Sea and the Yellow Sea have the largest output and are important small economic shrimps. Its meat is tender and delicious, and it is a high-protein, low-fat, mineral-rich food. In addition to serving fresh food, it can also be processed into sea rice, which is also known as "golden hook shrimp" because of its golden color. Its fertilized eggs can be dried to make shrimp roe, which is also an excellent dried seafood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6879C12Q1/6888C12N15/11
CPCC12Q1/6879C12Q1/6888
Inventor 吕建建李吉涛题兴斌刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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