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Kit for rapid detection of authenticity of edible sunflower hybrid sh361

A technology of SH361 and reagent kit, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of low incidence of sclerotinia, and achieve the effects of easy operation, efficient species identification, and accurate detection results

Active Publication Date: 2019-11-08
三瑞农业科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The variety is resistant to downy mildew and moderately resistant to Verticillium wilt; the incidence of Sclerotinia is low

Method used

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  • Kit for rapid detection of authenticity of edible sunflower hybrid sh361
  • Kit for rapid detection of authenticity of edible sunflower hybrid sh361
  • Kit for rapid detection of authenticity of edible sunflower hybrid sh361

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH361 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0060] The primer in the primer solution part is primer SR-57, and the SR-57 primer is:

[0061] SR-57-F: 5'-TTCCATTTCCACCATTTTGG-3';

[0062] SR-57-R: 5'-CATTCATGGCCTAAAAGGTTC-3'.

[0063] The formula of each described kit is:

[0064] Primer solution, 0.2μM, 1μL;

[0065] Taq DNA polymerase, 2.5units, 0.5μL;

[0066] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0067] dNTPs, 2.5mM, 2μL;

[0068] wxya 2 O, 17 μL.

[0069] The method for rapidly detecting the authenticity of the edible sunflower hybrid SH361 by using the above kit comprises the following steps:

[0070] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by the edible sunflower hybrid SH361 and its parent standard sunflower seeds A436 and ...

Embodiment 2

[0093] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH361 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0094] The primer in the primer solution part is primer SR-123, and the SR-123 primer:

[0095] SR-123-F:5'-GAAAACCCATGCAGGCATAC-3';

[0096] SR-123-R: 5'-ACATCCATCACAGTCCATTTTG-3'.

[0097] The formula of each described kit is:

[0098] Primer solution, 0.2μM, 1μL;

[0099] Taq DNA polymerase, 2.5units, 0.5μL;

[0100] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0101] dNTPs, 2.5mM, 2μL;

[0102] wxya 2 O, 17 μL.

[0103] The method for rapidly detecting the authenticity of the edible sunflower hybrid SH361 by using the above kit comprises the following steps:

[0104] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by the edible sunflower hybrid SH361 and its parent standard sunflower seeds A436 and...

Embodiment 3

[0127] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH361 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0128] The primer in the primer solution part is primer SR-830, and the SR-830 ​​primer:

[0129] SR-830-F: 5'-CAAGTGCATTAGGTGGTTCTAACA-3';

[0130] SR-830-R: 5'-GCCCTCTGACTGTTGTATGACTG-3'.

[0131] The formula of each described kit is:

[0132] Primer solution, 0.2μM, 1μL;

[0133]Taq DNA polymerase, 2.5units, 0.5μL;

[0134] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0135] dNTPs, 2.5mM, 2μL;

[0136] wxya 2 O, 17 μL.

[0137] The method for quickly identifying the authenticity of the edible sunflower hybrid SH361 using the kit comprises the following steps:

[0138] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by the edible sunflower hybrid SH361 and its parent standard sunflower seeds A436 and...

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Abstract

The invention provides a kit for rapid detection of authenticity of an edible sunflower hybrid SH361. The kit includes a primer liquid, a reaction liquid and a DNA polymerase portion, which are individually wrapped, wherein the primer liquid portion comprises at least one of the following primers: a primer SR-57, a primer SR-123, a primer SR-830, a primer SR-1040 and a primer SR-1164. The prime kit can test a large number of sunflower in a short time, and the detection results are accurate, stable and reliable.

Description

technical field [0001] The invention belongs to the field of identifying the authenticity and variety purity of crop seeds, and in particular relates to a kit for quickly detecting the authenticity of sunflower hybrid SH361 established based on SSR molecular marker technology. Background technique [0002] Sunflower (Helianthus annuus L.) is a dicotyledonous plant belonging to the genus Helianthus in the family Compositae. Native to southwestern North America, sunflower is the only crop that originated and domesticated in North America and is widely cultivated around the world. Sunflower is divided into oil-use type sunflower and edible type sunflower. The chromosome base number is 17, and there are different multiple levels of two times, four times, and six times. Among them, the cultivated species are diploid and belong to insect-borne cross-pollinated crops. Sunflower is a light-loving short-day plant. The effective accumulated temperature of the whole growth period is ≥...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
Inventor 姚梅园张永平马德甯司立平万县贞
Owner 三瑞农业科技股份有限公司
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