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A kind of identification method of channel catfish breeding pond fertilization method

A channel catfish and pond technology, applied in biochemical equipment and methods, microbial determination/inspection, climate change adaptation, etc., can solve problems such as labor cost differences, inability to identify fertilization methods, and achieve stable identification results and high accuracy. , the effect of low cost

Active Publication Date: 2022-07-05
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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Problems solved by technology

However, in China, the cost of fertilizers and labor costs are different from those in the United States. Therefore, there are differences in the use of inorganic fertilizers and organic fertilizers in aquaculture ponds.
At present, it is impossible to identify the method of fertilization in the breeding process of channel catfish

Method used

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  • A kind of identification method of channel catfish breeding pond fertilization method
  • A kind of identification method of channel catfish breeding pond fertilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] There are 9 test ponds of 1.5 mu, divided into 3 groups of organic fertilizer, inorganic fertilizer and no fertilization, and each group has 3 parallel groups. Before the start of pond culture, drain and sprinkle strong chlorine essence to clear the pond. After killing wild fish, add water to 1.2m. A week later, fertilization began on May 30. In the inorganic fertilizer group, urea (Jiangsu Linggu Chemical Group Co., Ltd., TN≥46%) and compound fertilizer (Henan Poly Chemical Fertilizer Co., Ltd., N:P 2 O 5:K 2 O= 20kg / ha N and 2kg / ha P) were applied. 16:16:16); the organic fertilizer group was applied with multifunctional biological type 1 aquatic special fertilizer (Jiangsu Lihua Biological Fertilizer Co., Ltd., total nutrients ≥ 5%, organic matter ≥ 54%), and 120kg per pond was applied according to the dosage instructions. After the fertilizer granules are dissolved in water, the whole pool is evenly sprinkled, and the dosage is halved in the 2nd to 4th weeks, and fe...

Embodiment 2

[0029] 20 channel catfish with organic fertilizers were collected from the ponds of Pukou and Yangzhong, Jiangsu, and 60 channel catfish with inorganic fertilizers were collected from the ponds of Dafeng, Jianhu, Xiangshui, Dongtai, Hai'an, Rudong, etc. . Before the start of breeding, fertilize according to conventional breeding management. The organic fertilizers used are bagged organic fertilizer granules produced by regular manufacturers in the market, and the inorganic fertilizers are commercial agricultural conventional bagged urea and compound fertilizers. The starting time of the sampling pond culture varies from mid-late March to mid-April, and the initial stocking weight is about 50g. The sampling time was from May to mid-July, 2-3 months after fertilization, and the average weight was 267g.

[0030] The intestinal contents of each channel catfish were collected in Tangkou, put into a centrifuge tube and quickly frozen in liquid nitrogen. After returning to the labo...

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Abstract

The invention provides a method for identifying the fertilization mode of channel catfish breeding ponds. The total DNA of the channel catfish intestinal microorganisms in the culture pond is extracted, and the total DNA is used as a template to target Lactobacillus spp. Lactobacillus The primers were designed for the DNA sequence of the 16S rDNA gene of the population, and PCR amplification was performed. The method for identifying the fertilization mode of the channel catfish pond according to the invention can quickly, simply, accurately and effectively identify the fertilization mode of the channel catfish culture pond, and has the advantages of simple operation, low cost, high accuracy, stable and reliable identification results, and can solve the problem of channel catfish cultivating ponds. Difficulty in identifying fertilization methods.

Description

technical field [0001] The invention relates to the technical field of aquatic organism identification, in particular to a method for identifying a fertilization method in a channel catfish culture pond. Background technique [0002] Channel catfish, also known as channel catfish, is native to North America and is the most important freshwater aquaculture fish in the United States. It has developed rapidly, and the annual output has exceeded 300,000 tons. Through the continuous improvement and innovation of my country's aquaculture industry, my country's channel catfish breeding scale and unit breeding output have comprehensively surpassed the United States and achieved better economic benefits. The first one in the aquaculture Bazi classics summed up by our country's aquatic science and technology workers is to emphasize water, and raising fish before raising water has become a prerequisite for aquaculture. The so-called water raising is to ensure that the pond culture wate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/686C12Q1/6888
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2565/125Y02A40/81
Inventor 钟立强王明华张世勇姜虎成陈校辉
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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