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Method for identifying aphid-resistant transgenic cotton

An identification method and genetically modified technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inapplicable transgenic cotton, achieve stable identification results, strong practicability and operability, and huge economic benefits Effect

Inactive Publication Date: 2011-03-02
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Therefore, the original aphid-resistant conventional cotton identification methods in my country are no longer suitable for transgenic cotton, so there is an urgent need to study aphid-resistant identification methods suitable for transgenic cotton

Method used

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  • Method for identifying aphid-resistant transgenic cotton
  • Method for identifying aphid-resistant transgenic cotton
  • Method for identifying aphid-resistant transgenic cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] experiment procedure:

[0022] (1) The experiment was carried out in a greenhouse. The aphid-sensitive variety Africa E40 provided by the Germplasm Resources Office of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences was used as the control species, and the R09-19 provided by the Biotechnology Research Office of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences was used. The transgenic cotton variety is the tested variety, and 10 pots of African E40 and R09-19 transgenic cotton were planted in flower pots with a height of 20 cm, a diameter of 8 cm at the bottom, and a diameter of 12 cm at the top, and 1 cotton seedling was left in each pot;

[0023] (2) Cotton is placed in diameter and is 30 centimetres, and height is in the cage of the belt cover of 70 centimeters, and 100 order nylon nets are attached outside the cage to prevent the invasion of pests and natural enemies;

[0024] (3) At the 3-leaf stage of cotto...

Embodiment 2-5

[0028] The test procedure is the same as that in Example 1, except that R09-20, R09-21, R09-23, and R09-24 provided by the Biotechnology Laboratory of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences were used as the tested varieties. The test results are also shown in Table 1.

[0029] test results:

[0030] a. Control species

[0031] After observation, the tooth damage grades of the control variety Africa E40 were 4, 3, 3, 3, 3, 2, 4, 3, 3, 2;

[0032] Calculated by formula I:

[0033] The aphid damage index of the control variety Africa E40 was 75.0%;

[0034]

[0035] b. Species under inspection:

[0036] After observation, the tooth damage grades of R09-19 transgenic cotton varieties are respectively 3, 2, 3, 2, 3, 2, 2, 3, 3, 0 grades, and the aphid damage of R09-19 transgenic cotton varieties is calculated according to formula I. The aphid damage index was 57.5%, and the aphid damage index reduction rate of the R09-19 transgenic cot...

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Abstract

The invention relates to a method for identifying an aphid-resistant transgenic cotton. The method comprises the following steps: planting control varieties and checked varieties in a flower pot with a cover at room temperature; carrying out artificial aphid inoculation on each control variety and each checked variety; investigating the number of cotton aphids on each plant of the cotton the third day after the artificial aphid inoculation is carried out; and taking the blade of the cotton plate most severely damaged as a standard leaf to investigate aphid harm magnitude of the control varieties and the check varieties the seventh day after the artificial aphid inoculation is carried out, and identifying the transgenic cotton variety resistant to the aphids according to the aphid hazard index and aphid hazard index deceasing rate. The method provided by the invention features good practicability and operability and stable and reliable identification result.

Description

technical field [0001] The invention relates to an identification method of aphid-resistant transgenic cotton. Background technique [0002] Cotton suffers from various diseases and insects throughout its life, and the loss of cotton due to diseases and insects is 15% to 20% all the year round, and more than 50% is lost in serious occurrence years, or even no harvest. In the past 20 years, the development of integrated control strategies for crop diseases and insect pests in my country has gone through two stages. The first stage is an integrated control strategy targeting a single pest during the "Sixth Five-Year Plan" period, and the second stage is from the "Seventh Five-Year Plan". "To the "Ninth Five-Year Plan" comprehensive control strategy of multi-pest complex groups with crops as the main body. However, we have not achieved sustainable control of crop pests and diseases, especially since the 1990s, some major pest disasters are still occurring frequently, and new pe...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G13/00A01G7/00
Inventor 雒珺瑜崔金杰马艳王春义张帅吕丽敏辛惠江李春花吴冬梅
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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