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Kit for rapid detection of authenticity of edible sunflower hybrid sh338

A kit, sunflower technology, applied in the field of kits that can quickly detect the authenticity of the sunflower hybrid SH338, to achieve the effect of clear electrophoretic patterns, simple operation steps, and efficient species identification

Active Publication Date: 2019-11-08
三瑞农业科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of SSR molecular markers to identify the authenticity and purity of edible sunflower hybrids. It is of great significance to establish a set of SSR molecular markers suitable for the identification of the authenticity and purity of edible sunflower hybrids, which can overcome the traditional Deficiencies brought about by field plot planting identification

Method used

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  • Kit for rapid detection of authenticity of edible sunflower hybrid sh338
  • Kit for rapid detection of authenticity of edible sunflower hybrid sh338
  • Kit for rapid detection of authenticity of edible sunflower hybrid sh338

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH338 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0054] The primer in the primer solution part is primer SR-123, and the SR-123 primer is:

[0055] SR-123-F:5'-GAAAACCCATGCAGGCATAC-3';

[0056] SR-123-R: 5'-ACATCCATCACAGTCCATTTTG-3'.

[0057] The formula of each described kit is:

[0058] Primer solution, 0.2μM, 1μL;

[0059] Taq DNA polymerase, 2.5units, 0.5μL;

[0060] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0061] dNTPs, 2.5mM, 2μL;

[0062] wxya 2 O, 17 μL.

[0063] The method for rapidly detecting the authenticity of the edible sunflower hybrid SH338 by using the above-mentioned kit comprises the following steps:

[0064] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by edible sunflower hybrid SH338 and its parent standard sunflower seeds...

Embodiment 2

[0087] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH338 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0088] The primer in the primer solution part is primer SR-889, and the SR-889 primer:

[0089] SR-889-F:5'-ATCAACTACGTCACGATACTCC-3';

[0090] SR-889-R: 5'-GTTCTCATGGATTCTCACAACTC-3'.

[0091] The formula of each described kit is:

[0092] Primer solution, 0.2μM, 1μL;

[0093] Taq DNA polymerase, 2.5units, 0.5μL;

[0094] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0095] dNTPs, 2.5mM, 2μL;

[0096] wxya 2 O, 17 μL.

[0097] The method for rapidly detecting the authenticity of the edible sunflower hybrid SH338 by using the above-mentioned kit comprises the following steps:

[0098] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by edible sunflower hybrid SH338 and its parent standard sunflower seeds...

Embodiment 3

[0121] The kit for rapid detection of the authenticity of the edible sunflower hybrid SH338 described in this embodiment includes separately packaged primer solutions, reaction solutions and DNA polymerase parts, wherein,

[0122] The primer in the primer solution part is primer SR-1114, and the SR-1114 primer:

[0123] SR-1114-F: 5'-AGATGGTGGCAGGAGAGTTAAAG-3';

[0124] SR-1114-R: 5'-GCAGAAACAGATCAGGAGGGTAT-3'.

[0125] The formula of each described kit is:

[0126] Primer solution, 0.2μM, 1μL;

[0127] Taq DNA polymerase, 2.5units, 0.5μL;

[0128] 10× Contains Mg 2+ Amplification buffer, 2mM, 2.5μL;

[0129] dNTPs, 2.5mM, 2μL;

[0130] wxya 2 O, 17 μL.

[0131] The method for quickly identifying the authenticity of the edible sunflower hybrid SH338 using the kit comprises the following steps:

[0132] (1) Using the improved CTAB method to extract the genomic DNA of the sunflower grown by edible sunflower hybrid SH338 and its parent standard sunflower seeds A03-6 and ...

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Abstract

The invention provides a kit for rapid detection of authenticity of an edible sunflower hybrid SH338. The kit includes a primer liquid, a reaction liquid and a DNA polymerase portion, which are individually wrapped, wherein the primer liquid portion comprises at least one of the following primers: a primer SR-123, a primer SR-889 and a primer SR-1114. The prime kit can test a large number of sunflower in a short time, and the detection results are accurate, stable and reliable.

Description

technical field [0001] The invention belongs to the field of identifying the authenticity and variety purity of crop seeds, and in particular relates to a kit for rapidly detecting the authenticity of sunflower hybrid SH338 established based on SSR molecular marker technology. Background technique [0002] Sunflower (Helianthus annuus L.) is a dicotyledonous plant belonging to the genus Helianthus in the family Compositae. Sunflower is divided into oil-use type, edible type and dual-purpose sunflower. The base number of chromosomes is 17, and there are different levels of double, quadruple, and six-fold. Species have been found in different multiple levels such as double, quadruple, and sextuple. Since the 1960s, sunflower has developed rapidly all over the world, and its oil production is second only to soybean. Sunflower is a light-loving short-day plant. The effective accumulated temperature of the whole growth period is ≥1800-2600°C. It is drought-resistant, saline-alk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
Inventor 司立平张永平马德甯姚梅园万县贞
Owner 三瑞农业科技股份有限公司
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