37results about How to "Short identification time" patented technology

The invention discloses a method for distinguishing green shell egg laying chicken and chicken green shell egg genotype as well as a special fragment and primers. The invention provides a method for identification or auxiliary identification of whether chicken to be detected is green shell egg chicken or not, in order to detect the genome DNA (deoxyribonucleic acid), if objective DNA molecules (sequence 4) are contained in the genome DNA, the chicken to be detected belongs to or is candidated as the green shell egg chicken, and when the objective DNA molecules are not contained in the genome DNA, the chicken to be detected does not belong to or is not candidated as the green shell egg chicken. Experiments prove that a 425bp short fragment is adopted, three primers are designed and synthesized according to the short fragment and are used for multiple PCR (polymerase chain reaction) amplification, whether the chicken to be detected lays green shell eggs or not can be distinguished through detecting whether amplification products contain the short fragment or not, and meanwhile, the chicken green shell egg genotype can be distinguished.

The invention discloses a bacteria identification kit, including indicator, culture medium and testing carbon sources. Thiazole blue is used as the indicator for indicating that whether bacteria can use the testing carbon sources. The invention is a kit which uses a 96-microtitor plate that is prearranged with the culture medium with 95 different carbon sources and is used for the rapid identification of carbon source utilization spectrum. One hole is not added with the carbon source for the comparison. The kit of the invention includes some carbon sources for detecting the specific physiological characteristics and common carbon sources. That using the thiazole blue as the indicator is convenient for naked eyes to observe that whether the bacteria grows and uses the carbon sources. And an eliasa can be used at the wavelength of 600nm to rapidly measure the growing condition of the bacteria. The kit of the invention has the advantages of low cost, short identification time, high sensitivity, convenient operation, accuracy, etc. the result can be made within 20-24 hours. And gram negative bacteria and positive bacteria can be identified.

The invention discloses a fingerprint spectrum of a butterfly-leaf platycladus orientalis SSR marker and a construction method and application thereof. The invention provides a set of primers, which are composed of a primer pair 1 to a primer pair 8; and the nucleotide sequences of the primer pair 1 to the primer pair 8 are respectively sequence 1 to sequence 16 in a sequence table. The SSR markersuitable for butterfly-leaf platycladus orientalis is developed based on platycladus orientalis transcriptome sequencing, and the construction method and application of the fingerprint spectrum of the butterfly-leaf platycladus orientalis SSR marker are established. Compared with morphological detection, the technology has the advantages that the identification result is not affected by climate,environment, human factors and tree age, identification and differentiation can be effectively carried out in the seedling stage, the identification time is short, the identification accuracy is high,the detection efficiency is high, the stability is good, and the technology has important significance and good application effect on genetic specificity identification of butterfly-leaf platycladusorientalis and other platycladus orientalis germplasm.

A process for preparing the seeds of multi-resistance transgenic rice includes transferring the anti-herbicide gene and the anti-borer gene to the restoring line of three-line or two-line crossed rice, transferring the plant hopper-resistant gene to the maintenance line of three-line crossed rice, hybridizing between sterile line and maintenance line, and hybridizing between transgenic restoring line and transgenic sterile line.

The invention discloses a method for quickly identifying the drought resistance of wheat by using the green recovering rate of leaves. Drought stress treatment is performed in a three-leaf period of wheat, the time from dryness to green recovering of the leaves and the ratio of the leaves which recover green are detected after rehydration, and the drought resistance of the wheat can be identifiedquickly through comparison and rating; the result of drought resistance identification is in good accordance with the result of a method for identifying drought resistance indexes. The method has theadvantages of rapidity, high efficiency, low cost, easy operation and the like, field water management of farmers can be guided during production, and through the cooperation with other drought resistance identifying methods in scientific researches, excellent drought-resistant genetic resources can be selected quickly in a batch of materials.

The invention discloses a method and a system for rapidly identifying the authenticity of seeds with seed coatings, and relates to the technical field of seed authenticity identification. The method comprises the steps of S1, acquiring a plurality of seeds to be identified, and cutting each seed to be identified into two equal halves, wherein each half is taken as a unit to be indentified; S2, acquiring near-infrared spectrums corresponding to the cut surfaces of the M units to be indentified every time, wherein the acquired near-infrared spectrums are taken as near-infrared spectrums of the seeds to be identified; and S3, carrying out feature extraction on the near-infrared spectrums of the seeds to be identified, comparing extracted features with a preset characteristic model, and determining the authenticity of the seeds to be identified according to the comparison results. According to the method and the system, the seeds to be identified are treated, the near-infrared spectrums of the cut surfaces of the seeds to be identified are acquired, and the seeds to be identified can be identified according to the near-infrared spectrums, so that the authenticity of the seeds can be identified by the near-infrared spectrums, and the identification time is short.

The invention provides a microbial identification system and method based on a Logistic four-parameter model and a MASCA algorithm. The system comprises a microbial culture part, an acquisition calculation part, a curve generating part and a microbial judgement part. The microbial culture part is provided with multiple incubation holes and used for culturing to-be-identified microorganisms, wherein the incubation holes are formed in an incubation plate, and a base liquid is placed in each incubation hole; the acquisition calculation part is provided with a CCD camera and a computer, wherein the CCD camera is used for acquiring an image of a bacterial liquid of the to-be-identified microorganisms in each incubation hole and obtaining the gray value of the image, and the computer is connected with the CCD camera and used for converting the gray values into microorganism bacterial liquid turbidity values on the basis of a predetermined rule; the curve generating part is used for generating a growth curve according to the turbidity values of the microorganism bacterial liquids during logarithmic growth period and the to-be-identified microorganisms in each incubation hole during corresponding growth time; the microbial judgement part is used for matching the growth curve with a standard growth curve of known microorganisms in a standard growth curve database and correspondingly obtaining the species of the to-be-identified microorganisms. According to the method, the microorganism identification system is adopted for identification, and finally the species of the to-be-identified microorganisms is obtained.

The invention discloses a DNA (deoxyribonucleic acid) bar code. The nucleotide sequence of the DNA bar code is as shown in SEQ ID NO.1. According to the DNA bar code, an ITS sequence of a muscadine isfirstly disclosed, available molecular markers are provided for species identification of the muscadine, and the DNA bar code further comprises parts of conserved sequences and has universality whenbeing used for species identification of grapes. The invention discloses a method and a primer for preparing the nucleotide sequence of the DNA bar code and can acquire sequences of an ITS area of themuscadine. The invention further discloses a molecular identification method of the muscadine and a detection primer and a kit for identifying the muscadine. PCR (polymerase chain reaction) amplification is performed by the aid of the detection primer or the kit to obtain sequences of ITS areas of the grapes, an amplification sequence and the sequence of the DNA bar code of the muscadine are compared or applied to construction of phylogenetic trees of the grapes, so that grape varieties are accurately identified, and identifying processes are simple to operate, high in efficiency and good inrepeatability.

Light (111) emitted from a light source (110) is transmitted through an objective lens (140), reflected from an optical disc (10), transmitted again through the objective lens (140), and thereafter divided into central light (111A) transmitted through a central part of the objective lens (140) and peripheral light (111P) transmitted through a peripheral part of the objective lens (140) by a hologram (150). A central light FE signal (S212A) is generated from the central light (111A) and a peripheral light FE signal (S212P) is generated from the peripheral light (111P). In a focus search operation, an S-letter detector (221A) detects a time point at a zero level of the central light FE signal (S212A), and an S-letter detector (221P) detects a time point at a zero level of the peripheral light FE signal (S212P). A time difference is measured by a timer counter (222) in terms of elapsed time. Disc identification means (300) identifies a type of the optical disc (10) based on the time difference.

The invention discloses an identification method of a Chinese rose botrytis cinerea physiological race. The method comprises the steps of: carrying out separation, purification and propagation expansion on botrytis cinerea; preparing Chinese rose botrytis cinerea spore suspension; adopting 6 parts of identification host materials; carrying out in-vitro petal identification; and grading petal disease conditions. According to the method, only by six parts of identification host materials, the Chinese rose botrytis cinerea physiological race can be efficiently and accurately identified in variousregions of China, the cost is saved, and labor and time are saved.

The invention belongs to the technical field of agricultural breeding, and provides a method for rapidly identifying tomato bacterial wilt seedling stage resistance by an injection inoculation method, wherein the method comprises the steps of tomato material seedling preparation, ralstonia solanacearum inoculation liquid preparation, injection inoculation and disease resistance observation and identification. The injection inoculation comprises the steps: when a tomato seedling grows to have 5-6 leaves, sucking ralstonia solanacearum inoculation bacterial liquid by using an injector, aligning a needle head of an injector to the middle of a first stem of the seedling, puncturing the stem along the inclined lower direction of the stem, returning the needle head to a position close to a puncturing point in the stem, and injecting the bacterial liquid until the bacterial liquid overflows from the stem. According to the method, the bacterial wilt resistance of a breeding population material can be rapidly identified in the seedling stage, the tomato bacterial wilt resistance breeding efficiency is improved, and a simple, convenient and rapid detection method can be provided for research on tomato bacterial wilt resistance germplasm resource screening, a disease-resistant molecular mechanism and the like.