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Identification method of Chinese rose botrytis cinerea physiological race

A technology for Botrytis cinerea and physiological races, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of time-consuming and laborious, long identification cycle, etc., and achieve the effect of strong identification ability and cost saving.

Inactive Publication Date: 2021-03-16
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

In view of the fact that our country still lacks a set of identification host system suitable for the identification of physiological races of Botrytis cinerea in various regions of the country, and the statistical method of host incidence used in the traditional resistance identification method is time-consuming, laborious and difficult to identify. Therefore, it is imminent to explore a system and method for identification of hosts that are accurate in identification and fast in screening, and are suitable for the identification of physiological races of Botrytis cinerea in China.

Method used

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  • Identification method of Chinese rose botrytis cinerea physiological race
  • Identification method of Chinese rose botrytis cinerea physiological race
  • Identification method of Chinese rose botrytis cinerea physiological race

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Embodiment 1

[0044] Embodiment 1 Identification of Physiological Races of Botrytis cinerea in my country

[0045] Since Yunnan Province is the distribution center of wild rose resources and the cultivation center of rose varieties in China, it is also the main occurrence place of gray mold in my country. Therefore, this implementation case mainly focuses on Yunnan Province, and covers other 6 provinces in China, with a total of 12 different regions as collection locations.

[0046] (1) Isolation, purification and propagation of Botrytis cinerea

[0047] ①On the ultra-clean workbench, use a sterilized dissecting needle (sterilization method: high temperature sterilization at 120°C for 20 minutes) to collect from rose petals infected with Botrytis cinerea from the following 12 different places in China, respectively Dip the botrytis cinerea and transfer it to the petals of the rose tissue culture seedlings in the tissue culture bottle cultivated according to the conventional plant tissue cu...

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Abstract

The invention discloses an identification method of a Chinese rose botrytis cinerea physiological race. The method comprises the steps of: carrying out separation, purification and propagation expansion on botrytis cinerea; preparing Chinese rose botrytis cinerea spore suspension; adopting 6 parts of identification host materials; carrying out in-vitro petal identification; and grading petal disease conditions. According to the method, only by six parts of identification host materials, the Chinese rose botrytis cinerea physiological race can be efficiently and accurately identified in variousregions of China, the cost is saved, and labor and time are saved.

Description

technical field [0001] The invention belongs to the technical field of disease-resistant breeding of woody flowers, and in particular relates to a method for identifying physiological races of Botrytis cinerea. Background technique [0002] Rose (Rosa hybrida) is a plant of the genus Rosa (Rosa L.) in the Rosaceae (Rosaceae), and it is currently the largest fresh-cut flower species in the world. The main production areas of my country's cut flower roses are concentrated in Kunming, Yunnan Province and central Yunnan. [0003] Botrytis cinerea is a fungal disease caused by Botrytis cinerea, and it is the most serious fungal disease in the transportation of cut roses. It not only infects stems and leaves, but is most harmful to flowers. At present, the occurrence of botrytis cinerea is mainly prevented and controlled by repeated application of chemical agents in production, which not only increases the production cost, but also causes pollution and damage to the environment,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 邱显钦张颢晏慧君蹇洪英陈敏唐开学王其刚张婷
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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