Primer and method for identifying low phytic acid rice mutant zju-lpa2 filial generation genetypes

Abstract

The invention discloses a primer and a method for identifying low phytic acid rice mutant zju-lpa2 filial generation genetypes, wherein the primer is a primer ZHJ1, a primer ZHJ2 or a combination of the ZHJ1 with the primer ZHJ2. The method includes: (1) extracting total DNA (deoxyribonucleic acid) of leaves of rice samples at the seedling stage; (2) with the total DNA serving as a template, performing PCR (polymerase chain reaction) amplification by the aid of the primer in patent claim 1, subjecting amplified products to gel electrophoretic separation, and imaging after dyeing; and (3) judging the genetypes of the rice samples according to electrophoresis results. The primer co-separated from low phytic acid rice mutant zju-lpa2 can detect the leaves of the rice samples at the seedling stage, so that selection efficiency of LPA (low phytic acid) rice varieties can be greatly improved, and LPA rice breeding process is accelerated.

Description

technical field [0001] The invention belongs to the technical field of agricultural bioengineering, and in particular relates to a primer and a method for identifying the genotype of the rice low-phytic acid mutant zju-lpa2 hybrid progeny. Background technique [0002] Phytic acid is the main storage form of phosphorus in plant seeds, accounting for about 65-85% of the total phosphorus in seeds. Phytic acid is usually combined with Zn 2+ , Ca 2+ , Fe 3+ Such metal cations are chelated into phytate, and further form a spherical complex with protein, which is deposited in the aleurone layer of grass crop grains. Since humans and non-ruminant animals (pigs, chickens, fish, etc.) do not contain phytase, it is difficult to digest and utilize the ingested phytic acid, which greatly reduces the bioavailability of phosphorus, and at the same time, phytic acid also affects trace elements and protein. And starch bioavailability, is a typical anti-nutritional factor. In addition, ...

AI Technical Summary

Problems solved by technology

However, at present the main ways to obtain LPA mutant lines are physical and chemical mutagenesis and natural screening, and the inorganic phosphorus content of individual seeds can only be detected by micro-measurement methods after harvesting mature seeds of crops, and the high inorganic phosphorus strains are initially screened. The experimental conditions further measure the content of phytate phosphorus and total phosphorus by methods such as iron precipitation, the breeding cycle is longer, the cost is high, and the efficiency is low

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