Method for identifying Vietnam camellia oleifera, Camellia odorata, Camellia frutescens and common Camellia oleifera
A technology of Camellia oleifera and Camellia oleifera is applied in the fields of Camellia oleifera, common Camellia oleifera, Fragrant Camellia oleifera, and Camellia oleifera.
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[0033] Example 1: Molecular identification between different Camellia oleifera species germplasm resources
[0034] 1. Select experimental materials
[0035] The Camellia oleifera species from the Camellia oleifera group distributed in South China were taken. Among them, Camellia oleifera from Vietnam came from 7 populations in Hainan Province, 2 populations in Guangdong Province, and 3 populations in Guangxi Zhuang Autonomous Region. From 2 populations in Guangxi Zhuang Autonomous Region, common camellia came from 2 populations in Guangxi Zhuang Autonomous Region and 1 population in Jiangxi Province, each population sequenced 5 samples, a total of 90 samples. For SNP and Indel specific marker validation.
[0036] 2. SNP and Indel marker detection
[0037] 2.1 Using the improved CTAB method to extract the total DNA of the above experimental materials Camellia oleifera samples;
[0038] 2.2 Use the DNA of the Camellia oleifera sample to be tested extracted in step 2.1 as the...
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