24 results about "Botryotinia polyblastis" patented technology

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The present application provides a carvacrol tertiary amine derivative, a preparation method and an application in preparing a plant source fungicide, the carvacrol tertiary amine derivative contains a cyclic secondary amine substituent, the preparation method of the derivative is that an intermediate II is dissolved in ethanol, then a cyclic secondary amine is added, heating and stirring are carried out, reflux is kept, after TLC detection reaction is completed, ethanol is removed under reduced pressure, then extraction, water washing and purification are carried out, and the carvacrol tertiary amine derivative is prepared. The carvacrol tertiary amine derivative prepared by the present application exhibits obvious bacteriostatic activity on cucumber anthracnose fungus, tomato botrytis blight fungus, wheat scab fungus and wheat take-all fungus. The preliminary bacteriostatic activity of carvacrol tertiary amine derivatives 1, 3, 10 and 14 on wheat take-all fungus is 60.8%-83.2%, which is significantly better than that of positive control hymexazol (48.6%) and reference control carvacrol (45.0%).

The invention belongs to the technical field of agricultural biology, and particularly relates to application of ningnanmycin salt in inhibiting phytopathogen. Derivatives of ningnanmycin salt are developed on the basis of ningnanmycin, and the ningnanmycin salt is applied to resisting ascomycophyta and oomyceta phytopathogens including valsa mali, pyricularia grisea, phytophthora capsici, physalospora piricola, botrytis cinerea, fusarium graminearum and the like. Experiments show that the ningnanmycin salt significantly improves the inhibitory activity of ningnanmycin on phytopathogens, more effectively inhibits various phytopathogens, realizes a wider-spectrum bactericidal effect, and has a good application prospect.

The invention provides a heterocyclic ring-containing nicotinamide compound, a synthesis method and application of the heterocyclic ring-containing nicotinamide compound in prevention and treatment of plant fungal diseases, and relates to the technical field of pesticides. The heterocyclic ring-containing nicotinamide compound is synthesized by using easily available raw materials and a simple, practical and low-cost synthesis method. The compound has a synergistic effect on rhizoctonia solani, magnaporthe oryzae, mango anthracnose pathogen, rubber anthracnose pathogen, litchi anthracnose pathogen, banana anthracnose pathogen, coffee anthracnose pathogen, fusarium graminearum, wheat powdery mildew pathogen, valsa mali, botrytis cinerea, sclerotinia sclerotiorum, sigatoka musae, banana scab pathogen, apple ring spot pathogen, botrytis cinerea, botrytis cinerea, botrytis cinerea, botrytis cinerea and botrytis cinerea. The compound has an excellent inhibiting effect on plant fungal diseases caused by fungi such as tobacco alternaria alternata, cotton rhizoctonia solani, exserohilum turcicum and hevea brasiliensis, is novel in structure, and has huge potential for creation of novel pesticides.

The invention provides an RcERF98 gene and application of the RcERF98 gene to enhancement of gray mold resistance of Chinese roses, and belongs to the technical field of biological prevention and control. The nucleotide sequence of the RcERF98 gene is as shown in SEQ ID NO: 1. The RcERF98 gene is silent and overexpressed in petals which are good in growth state and free of botrytis cinerea infection of Chinese roses, and research results show that the petals treated by the silent RcERF98 gene are larger in scab area, higher in ion permeability, lower in hydrogen peroxide content and higher in ascorbic acid content, and the resistance of the petals of the Chinese roses to the botrytis cinerea is reduced; the petals treated by the overexpressed RcERF98 gene are smaller in scab area, lower in ion permeability, higher in hydrogen peroxide content and lower in ascorbic acid content, and the resistance of the Chinese rose petals to gray mold is enhanced.

The application discloses SlWRKY51 The application belongs to the technical field of bioengineering, and relates to application of a gene in improving disease resistance of a tomato plant. SlWRKY51 The application is achieved by constructing a gene overexpression vector, transforming a transgenic positive strain through mediation of agrobacterium, and performing a grey mold infection experiment. SlWRKY51 Compared with a wild type, the transgenic positive strain shows significantly enhanced disease resistance after being infected by the grey mold. Further real-time fluorescent quantitative PCR detection data show that the expression amount of PR family and defense-related genes in the transgenic positive strain is significantly up-regulated, and it is confirmed that the gene can enhance the disease resistance of the tomato by regulating multiple defense signal pathways, and important gene resources are provided for tomato disease resistance molecular breeding.

The application discloses application of RcARF8 genes in enhancing resistance of Chinese rose to botrytis blight, and a nucleotide sequence of the RcARF8 gene is shown as SEQ ID NO. 1. In the application, the RcARF8 gene is silenced and overexpressed in petals of Chinese rose in a good growth state and free from infection of botrytis blight, and it is found that petals treated by silencing the RcARF8 gene have a larger disease spot area and a reduced resistance to botrytis blight, and petals treated by overexpressing the RcARF8 gene have enhanced resistance to botrytis blight.

The invention relates to a novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound, a preparation method thereof and application of the novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound as an antifungal drug. The novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound has an inhibition effect on parasite sphaerospora, botrytis cinerea, rhizoctonia solani, fusarium graminearum and candida albicans in the antifungal activity of the novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound. An antifungal biological activity result shows that the compound shows remarkable antifungal activity, has comprehensive antifungal characteristics and is determined as a broad-spectrum antifungal candidate, the EC50 value of the compound to parasitic powdery spores is superior to that of a positive control drug chlorothalonil, and the compound shows about 12 times and 25 times of titer advantages compared with famoxadone and carbendazim respectively. The spiro structure and multiple active groups of the compound can significantly improve the antifungal activity of the compound, and in addition, the compound has protection and treatment effects on powdery parasitic spore infection in corn, which shows that the compound has agricultural application potential.

The invention discloses an N-pyrazole-4-yl benzamide derivative of a diphenyl ether fragment containing a halogen-mixed substituent group and a preparation method and application thereof, and relates to the field of pesticide bactericides, and the structural general formula is as shown in formula (I). A bacteriostatic activity test is carried out on plant pathogenic fungi by using the pyrazole amide derivative disclosed by the invention, and a test result shows that the compounds have a good bacteriostatic effect on the plant pathogenic fungi such as pyricularia oryzae, fusarium graminearum, cucumber target leaf spot bacteria, rhizoctonia solani and botrytis cinerea; the invention provides a direction for further development of a novel succinate dehydrogenase inhibitor.

The invention belongs to the technical field of fermentation engineering and plant disease prevention and control, and particularly relates to pseudomonas veronii and application thereof in prevention and control of plant fungal diseases. The invention discloses Pseudomonas veronii JD003 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36825. The test shows that the Pseudomonas veronii JD003 can effectively inhibit the growth of tomato root rot, grape botrytis cinerea, colletotrichum capsici and alternaria capsici, and can effectively inhibit the growth of the tomato root rot, the grape botrytis cinerea, the colletotrichum capsici and the alternaria capsici. The compound provided by the invention has the advantages that the inhibition effects on the corresponding pathogenic microorganisms such as fusarium solani, botrytis cinerea, colletotrichum oxysporum and alternaria hexabarzicola respectively reach 64.7%, 72.9%, 74.1% and 73.8%, the compound has a good inhibition effect on pepper anthracnose, the field prevention and control effect reaches 83.5%, and a new direction is found for preventing and controlling plant diseases.

The invention discloses a cinnamic acid butenolide compound and a preparation method and application thereof.The cinnamic acid butenolide compound has the advantages of being environmentally friendly and low in toxicity, shows excellent performance in the field of insect killing and sterilization, can be widely applied to the scenes of agricultural pest control, forestry insect prevention, daily chemical antibiosis and the like, and has a wide application prospect. The compound disclosed by the invention can be used for remarkably inhibiting strawberry botrytis cinerea and colletotrichum gloeosporioides, the highest inhibition rate reaches 78%, in addition, the compound has a super-strong inhibition effect on alfalfa aphids, the inhibition rate of most compounds reaches 80% or above, the highest inhibition rate reaches 95%, and the compound has an important application value in the fields of agricultural chemistry and biological control. Besides, the preparation method is simple to operate and easy to realize through a commercialized cinnamic acid compound, does not need intermediate separation and purification, can directly and quickly synthesize a target product, and has the advantages of environmental friendliness, low toxicity, high yield, simple steps, mild conditions, wide substrate application range, simple post-treatment and the like.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a method for preventing and treating plant diseases by using bacillus velezensis, which comprises the following steps: S1, strain culture and identification; the method comprises the following steps: taking a tomato greenhouse soil sample, separating bacterial strains by adopting a dilution method and a plate coating method, and screening bacterial strains with an inhibiting effect on pathogenic bacteria by adopting a plate confrontation method; the pathogenic bacteria comprise any one or more of fusarium graminearum, alternaria solani, cucumber botrytis cinerea, cucumber bacterial angular leaf spot bacteria, phytophthora capsici and peronophythora litchii. The screened strains with high bacteriostatic activity are identified as follows: morphological observation: the bacillus velezensis forms milk white and rough bacterial colonies with irregular edges on a TSA culture medium, and the thalli are gram-positive rod-shaped and are used for generating endophytic spores; the invention can alleviate the problems of environmental pollution, pesticide residue, pathogen resistance and the like caused by the existing chemical pesticide for preventing and treating plant diseases.

This invention discloses a method for detecting resistance to methoxyacrylate (QoIs) fungicides caused by single-base mutations in *Botrytis cinerea*, the causal agent of grape gray mold. This invention is based on the RPA-CRISPR / Cas12a system and designs a method targeting the fungicide. cytb This invention relates to a rapid, simple, and specific method for detecting resistance to Botrytis cinerea using a gene containing specific primer pairs with mutation sites and specific crRNA for activating Cas12a cleavage activity, combined with a fluorescent reporter probe. The method allows for real-time field detection without complex instruments, demonstrating promising application prospects and providing guidance for the healthy development of the grape industry and the scientific prevention of Botrytis cinerea.

The invention discloses a method for detecting single base mutation of botrytis cinerea on methoxy acrylate (QoIs) bactericides so as to generate drug resistance. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair which aims at a bactericide-targeted cytb gene and comprises a mutation site and specific crRNA for activating Cas12a cutting activity are designed, and a rapid, simple and specific naked-eye visual output detection method for producing drug resistance to botrytis cinerea is realized in combination with a fluorescent report probe. The detection method provided by the invention can realize field instant detection without complex instruments and equipment, has a good application prospect, and provides medication guidance for healthy development of the grape industry and scientific prevention of gray mold.

The application relates to the technical field of chemical synthesis, and particularly discloses a synthesis method of a triplet carbene-mediated C2-substituted bicyclo[1.1.1]pentane derivative, which successfully opens up a synthesis path of C2-substituted bicyclo[1.1.1]pentane (BCP) by inserting bicyclo[1.1.0]butane (BCB) under photosensitive conditions; the 1,4-dibasic radical species generated by the triplet carbene has a faster recombination rate; the method not only lays a solid foundation for the application of bicyclo[1.1.1]pentane in medicinal chemistry, but also overcomes the limitation of traditional benzene rings in metabolic stability and water solubility by replacing a bioisostere and using a three-dimensional bridged ring structure. In addition, the synthesized derivatives exhibit excellent biological activity, and experimental data prove that the derivatives have significant fungal inhibition activity in tests such as botrytis cinerea, thereby providing important technical support for the design and development of new drugs.

The invention belongs to the field of pesticides, and discloses a compound with a structure as shown in a formula I. A preparation method of the compound comprises the following steps: sequentially carrying out hydrolysis, hydrazide and cyclization on methyl o-aminonicotinate to obtain the pyridopyrimidone derivative. The pyridopyrimidone derivative is a novel compound, has an inhibiting effect on gibberellic disease bacteria, rhizoctonia solani and botrytis cinerea, and can be used for preventing and treating plant diseases caused by fungi.

The application belongs to the technical field of microorganisms, and particularly relates to a biological sterilization composition containing acid-resistant strains and application thereof. The biological sterilization composition containing acid-resistant strains is characterized in that an effective component is compounded from a microbial active component and a chemical active component; the microbial active component is prepared from acid-resistant strains TXB1-10, and the chemical active component is benzene ether carbendazol; the taxonomic name of the acid-resistant strain TXB1-10 is Sinomonas atrocyanea, and the strain is preserved in the China Center for Type Culture Collection on December 28, 2020, with a preservation number of CCTCC No: M2020984. When the strain TXB1-10 is compounded with benzene ether carbendazol after being prepared into a microbial active component, the strain exhibits a synergistic effect on inhibiting the growth of tomato botrytis cinerea mycelium, and compared with a single effective component, the strain can improve the prevention and treatment effect on tomato botrytis cinerea, and can provide support for developing biological pesticides for preventing and treating tomato botrytis cinerea.

The invention relates to a fluorine sulfonylation preparation method of alkenyl sulfide and application of the fluorine sulfonylation preparation method in plant bacteriostasis, and belongs to the technical field of plant bacteriostasis. According to the present invention, the compound is constructed by using FSO2Cl to carry out a fluorosulfonylation reaction on alkynyl sulfide, and a shuttling HAT process is adopted to achieve the regioselective and stereoselective hydrofluorosulfonation reaction of alkynyl sulfide, such that the compound is more competitive than the unpopular ATRA process; an antifungal test shows that the compound has a remarkable inhibition effect on botrytis cinerea, botrytis cinerea and colletotrichum gloeosporioides, and a good basis can be provided for subsequent related researches.

The invention discloses a maltol-based derivative and application thereof, and the maltol-based derivative has an excellent antibacterial effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali, apple ring spot virus and the like, and can be used for preventing and treating various plant fungal diseases.