66 results about "Botryotinia polyblastis" patented technology

The invention discloses an application of an RcWRKY31 gene in enhancing the resistance of Chinese rose to gray mold. The nucleotide sequence of the RcWRKY31 gene is as shown in SEQ ID NO. 1. The RcWRKY31 gene is silenced and overexpressed in petals which are good in Chinese rose growth state and free of botrytis cinerea infection, and research results show that the petals treated by the silent RcWRKY31 gene are larger in scab area and lower in resistance to botrytis cinerea; the resistance of petals treated by overexpression of the RcWRKY31 gene to gray mold is enhanced.

The invention discloses a method for detecting drug resistance of botrytis cinerea to succinate dehydrogenase (SDHI) bactericides. On the basis of an RPA-CRISPR / Cas12a system, a specific primer pair amplification target fragment and crRNA for activating Cas12a trans-cleavage activity are designed aiming at key mutation sites related to drug resistance in a bactericide action target SdhB gene, so that fluorescent light visible to naked eyes can be generated only when a resistant strain sequence is matched with the crRNA, and the fluorescent light is opposite to the fluorescent light generated when a sensitive strain sequence is matched with the crRNA. The method provided by the invention overcomes the dependence on laboratories and large instruments in the traditional drug resistance detection method, provides key technical support for accurate prevention and control and drug resistance management of grape gray mold, and provides direct drug use basis for scientific prevention and control in the field.

A preparation method of plant compound essential oil comprises the following steps: preparing mixed powder from dried lemon slices, mint and litsea coreana, carrying out carbon dioxide supercritical extraction, and collecting an extract; the preparation method comprises the following steps: dropwise adding a carboxymethyl-beta-cyclodextrin solution into a mixture of eugenol and thymol, carrying out ultrasonic treatment to obtain an ultrasonic mixed solution, standing, centrifuging, collecting supernate, and drying to obtain a white powdery inclusion compound; and adding lecithin, Tween 80, disodium pyrophosphate and deionized water into the extract, heating and stirring, continuing to add the inclusion compound, homogenizing to obtain a nano-emulsion, adding gelatin and propylene glycol, heating and preserving heat, and cooling to obtain the plant composite essential oil. The compound essential oil disclosed by the invention has antibacterial and antioxidant effects, and has relatively strong antibacterial ability on botrytis cinerea and penicillium germs, and the DPPH free radical scavenging rate is greater than or equal to 90%. According to the invention, the active ingredients of the essential oil are wrapped by carboxymethyl-beta-cyclodextrin and synergistically embedded by lipidosome through a nano-embedding technology to realize slow release, so that the antioxidant and antibacterial properties are effectively prolonged.

The invention discloses low-temperature-resistant rice endophytic streptomyces with biological prevention and control effects on common crop diseases and application of the low-temperature-resistant rice endophytic streptomyces, relates to the field of microorganisms, and aims to solve the problems that an existing rice blast biocontrol strain cannot play a stable effect under a low-temperature condition and is poor in rice blast control effect. The low-temperature-resistant rice endophytic streptomycete is alfalfa streptomycete F05 and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 17, 2025, and the preservation number is CGMCC No.33539. The streptomyces medicaginis F05 disclosed by the invention can stably play a biological prevention and control role at a low temperature of 5-15 DEG C. The compound has broad-spectrum antibacterial performance, is used for inhibiting corn stalk rot bacteria, green bean colletotrichum, rice bakania, botrytis cinerea and rhizoctonia solani, and can also be used for decomposing starch and cellulose.

The invention discloses an application of an SlWRKY51 gene in improvement of tomato plant disease resistance, and belongs to the technical field of bioengineering. A transgenic positive strain is obtained through construction of an SlWRKY51 gene overexpression vector and agrobacterium-mediated transformation. A botrytis cinerea infection experiment shows that compared with a wild type, the transgenic positive strain shows remarkably enhanced disease resistance after being infected by botrytis cinerea. The further real-time fluorescent quantitative PCR detection data shows that the expression quantities of the PR family and defense-related genes in the transgenic positive strain are remarkably increased, which proves that the SlWRKY51 gene can enhance the disease resistance of the tomato by regulating multiple defense signal pathways, and an important gene resource is provided for tomato disease-resistant molecular breeding.

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The present application provides a carvacrol tertiary amine derivative, a preparation method and an application in preparing a plant source fungicide, the carvacrol tertiary amine derivative contains a cyclic secondary amine substituent, the preparation method of the derivative is that an intermediate II is dissolved in ethanol, then a cyclic secondary amine is added, heating and stirring are carried out, reflux is kept, after TLC detection reaction is completed, ethanol is removed under reduced pressure, then extraction, water washing and purification are carried out, and the carvacrol tertiary amine derivative is prepared. The carvacrol tertiary amine derivative prepared by the present application exhibits obvious bacteriostatic activity on cucumber anthracnose fungus, tomato botrytis blight fungus, wheat scab fungus and wheat take-all fungus. The preliminary bacteriostatic activity of carvacrol tertiary amine derivatives 1, 3, 10 and 14 on wheat take-all fungus is 60.8%-83.2%, which is significantly better than that of positive control hymexazol (48.6%) and reference control carvacrol (45.0%).

The application discloses a preparation method and application of an indole oxadiazole aryl thiourea derivative, and has the structure shown in the following general formula: The compound has a good inhibiting effect on pathogenic bacteria, and has a good inhibiting effect on pathogenic bacteria such as Xanthomonas campestris, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. mori, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. viticola, Xanthomonas axonopodis pv. vesicatoria, Xanthomonas axonopodis pv. actinidiae, Xanthomonas axonopodis pv. apodrecedens, Botrytis cinerea, Fusarium oxysporum f. sp. cubense, Sclerotinia sclerotiorum, Gibberella zeae, Phytophthora infestans, Monilinia fructicola, and the like, and provides an important scientific basis for research and development of new pesticides.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of ningnanmycin salt in inhibiting phytopathogen. Derivatives of ningnanmycin salt are developed on the basis of ningnanmycin, and the ningnanmycin salt is applied to resisting ascomycophyta and oomyceta phytopathogens including valsa mali, pyricularia grisea, phytophthora capsici, physalospora piricola, botrytis cinerea, fusarium graminearum and the like. Experiments show that the ningnanmycin salt significantly improves the inhibitory activity of ningnanmycin on phytopathogens, more effectively inhibits various phytopathogens, realizes a wider-spectrum bactericidal effect, and has a good application prospect.

The invention provides a heterocyclic ring-containing nicotinamide compound, a synthesis method and application of the heterocyclic ring-containing nicotinamide compound in prevention and treatment of plant fungal diseases, and relates to the technical field of pesticides. The heterocyclic ring-containing nicotinamide compound is synthesized by using easily available raw materials and a simple, practical and low-cost synthesis method. The compound has a synergistic effect on rhizoctonia solani, magnaporthe oryzae, mango anthracnose pathogen, rubber anthracnose pathogen, litchi anthracnose pathogen, banana anthracnose pathogen, coffee anthracnose pathogen, fusarium graminearum, wheat powdery mildew pathogen, valsa mali, botrytis cinerea, sclerotinia sclerotiorum, sigatoka musae, banana scab pathogen, apple ring spot pathogen, botrytis cinerea, botrytis cinerea, botrytis cinerea, botrytis cinerea and botrytis cinerea. The compound has an excellent inhibiting effect on plant fungal diseases caused by fungi such as tobacco alternaria alternata, cotton rhizoctonia solani, exserohilum turcicum and hevea brasiliensis, is novel in structure, and has huge potential for creation of novel pesticides.

The invention provides an RcERF98 gene and application of the RcERF98 gene to enhancement of gray mold resistance of Chinese roses, and belongs to the technical field of biological prevention and control. The nucleotide sequence of the RcERF98 gene is as shown in SEQ ID NO: 1. The RcERF98 gene is silent and overexpressed in petals which are good in growth state and free of botrytis cinerea infection of Chinese roses, and research results show that the petals treated by the silent RcERF98 gene are larger in scab area, higher in ion permeability, lower in hydrogen peroxide content and higher in ascorbic acid content, and the resistance of the petals of the Chinese roses to the botrytis cinerea is reduced; the petals treated by the overexpressed RcERF98 gene are smaller in scab area, lower in ion permeability, higher in hydrogen peroxide content and lower in ascorbic acid content, and the resistance of the Chinese rose petals to gray mold is enhanced.

The application discloses SlWRKY51 The application belongs to the technical field of bioengineering, and relates to application of a gene in improving disease resistance of a tomato plant. SlWRKY51 The application is achieved by constructing a gene overexpression vector, transforming a transgenic positive strain through mediation of agrobacterium, and performing a grey mold infection experiment. SlWRKY51 Compared with a wild type, the transgenic positive strain shows significantly enhanced disease resistance after being infected by the grey mold. Further real-time fluorescent quantitative PCR detection data show that the expression amount of PR family and defense-related genes in the transgenic positive strain is significantly up-regulated, and it is confirmed that the gene can enhance the disease resistance of the tomato by regulating multiple defense signal pathways, and important gene resources are provided for tomato disease resistance molecular breeding.

The invention relates to novel acridine spiroisoxazole compounds, a preparation method thereof and application of the novel acridine spiroisoxazole compounds in antifungal drugs. The novel acridine spiroisoxazole compounds have the antibacterial activity of 36 compounds, and the 36 compounds have the inhibiting effect on botrytis cinerea, parasite scalaria, rhizoctonia solani, fusarium graminearum and candida albicans. The antifungal biological activity result shows that the compound series all show obvious antibacterial activity. The compound is determined as a broad-spectrum antibacterial candidate due to the comprehensive antibacterial characteristics of the compound, and the EC50 value (5.0 + / -0.4 mu g / mL) of H35 shows 1.5 times, 2.2 times and 3.2 times of titer advantages compared with positive control drugs famoxadone (7.6 mu g / mL), chlorothalonil (11.1 mu g / mL) and carbendazim (15.9 mu g / mL) respectively. The spiro structure of the compound can significantly improve the antibacterial activity of the compound, the preliminary mechanism research target shows that the compound is related to fungal membrane damage, and in addition, the compound H35 has protection and treatment effects on botrytis cinerea infection in corn, which shows that the compound H35 has agricultural application potential.

The invention discloses an RcWRKY31 gene and application of the RcWRKY31 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY31 gene is shown as SEQ ID NO. 1. The RcWRKY31 gene is silenced and overexpressed in petals which are good in Chinese rose growth state and free of botrytis cinerea infection, and research results show that the petals treated by the silent RcWRKY31 gene are larger in scab area and lower in resistance to botrytis cinerea; the resistance of petals treated by overexpression of the RcWRKY31 gene to gray mold is enhanced.

The invention discloses a flavonol derivative containing a benzimidazole active fragment and application of the flavonol derivative. The flavonol derivative has the advantages of being low in raw material cost, simple and convenient in synthetic route and the like. The obtained flavonol derivative has an excellent bacteriostatic effect on botrytis cinerea and can be used for preventing and treating botrytis cinerea diseases.

The invention provides application of banana root exudates or an ethanol extract of the banana root exudates in preparation of 10-hydroxydecanoic acid. The new streptomyces is used for inducing the change of banana root exudates, the key root exudates 10-hydroxydecanoic acid can be obtained through separation, gibberellic disease and the like can be effectively antagonized, but no inhibition effect is achieved on banana fusarium oxysporum fourth physiological race and the like; however, the 10-hydroxydecanoic acid can effectively inhibit the fourth physiological race of the banana wilt pathogen in the banana rhizosphere soil, effectively prevent and treat the banana wilt caused by the fourth physiological race of the banana wilt pathogen, improve the abundance of Sphingomonas bacteria, Bacillus bacteria, Pseudolaabrys bacteria and the like in the banana rhizosphere soil, and improve the yield of the banana wilt disease caused by the fourth physiological race of the banana wilt pathogen and the banana wilt disease caused by the fourth physiological race of the banana wilt pathogen. The invention provides a new method and idea for preventing and controlling fusarium wilt and preventing and controlling wheat gibberellic disease, strawberry colletotrichum gloeosporioides, banana colletotrichum gloeosporioides, litchi colletotrichum gloeosporioides, mango colletotrichum gloeosporioides, grape botrytis

The invention relates to the technical field of medicinal chemistry, and discloses a spiro [indene-indoline] compound, a preparation method thereof and an application of the spiro [indene-indoline] compound as a bactericide, in the air atmosphere, benzaldehyde compounds derived from the third position of indole and amine compounds are mixed, a solvent is added, under the action of a catalyst, stirring reaction is performed at the temperature of 25-60 DEG C, and the spiro [indene-indoline] compound is obtained. The structural formula of the spiro [indene-indoline] compound is shown as a formula I in the specification. The spiro [indene-indoline] compound prepared by the invention has excellent inhibitory activity on four common pathogenic fungi: strawberry botrytis cinerea, valsa mali, cucumber fusarium wilt and peanut root rot, and a new solution is provided for overcoming the drug resistance of fungi, so that the spiro [indene-indoline] compound has important application value in the field of agricultural germ control.

The application discloses application of RcARF8 genes in enhancing resistance of Chinese rose to botrytis blight, and a nucleotide sequence of the RcARF8 gene is shown as SEQ ID NO. 1. In the application, the RcARF8 gene is silenced and overexpressed in petals of Chinese rose in a good growth state and free from infection of botrytis blight, and it is found that petals treated by silencing the RcARF8 gene have a larger disease spot area and a reduced resistance to botrytis blight, and petals treated by overexpressing the RcARF8 gene have enhanced resistance to botrytis blight.

The invention relates to a sulfonamide compound containing thiazole heterocycle as well as a preparation method and application of the sulfonamide compound, and belongs to the technical field of agricultural chemicals and bactericides. According to the invention, substituted sulfonyl chloride and unsubstituted or substituted aminoacetophenone are used as main raw materials, the thiazole heterocyclic ring and a sulfonamide group are effectively combined through different reactions to prepare the novel sulfonamide compound containing the thiazole heterocyclic ring, and the structural general formula is shown as a formula I or a formula II. The compound provided by the invention has bacteriostatic activity on multiple plant pathogenic fungi such as sclerotinia sclerotiorum, rhizoctonia solani, botrytis cinerea, curvularia maydis, fusarium oxysporum, pyricularia grisea and the like, and an innovative solution is provided for agricultural sustainable disease prevention and control.

The invention discloses Cunninghamella elegans and application of the Cunninghamella elegans, the strain number of the Cunninghamella elegans is JSAFC 2203, the Cunninghamella elegans is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC No.41973. The Cunninghamella elegans has the advantages that the Cunninghamella elegans can be used for preparing the Cunninghamella elegans; according to the present invention, the soil around the blueberry is subjected to separation and purification so as to obtain the Cunninghamella elegans (Cunninghamella elegans); the bacterial strain can be used as a biocontrol bacterial strain and has inhibitory activity on Botrytis cinerea, and the inhibition rate of plate confrontation culture reaches 77.42%. Meanwhile, the compound has a growth promoting effect on blueberry plants.

The invention discloses an antibacterial and fresh-keeping methyl jasmonate compound fresh-keeping agent for passion fruits as well as a preparation method and application of the antibacterial and fresh-keeping methyl jasmonate compound fresh-keeping agent. The effective components of the methyl jasmonate compound fresh-keeping agent consist of methyl jasmonate, Tween 80 and chitosan. According to the present invention, the methyl jasmonate and the Tween 80 are uniformly fused through the special compounding process, and the chitosan is added to form the methyl jasmonate composite fresh-keeping agent, such that the experiment verification results show that the methyl jasmonate composite fresh-keeping agent has significant effects on maintaining the nutritional components of the fruits and delaying senescence, and can prolong the fresh-keeping period; common diseases in the storage period, such as botrytis cinerea, penicillium germs and the like, can be effectively inhibited. The methyl jasmonate compound preservative disclosed by the invention is simple in preparation process, convenient to use, safe and environment-friendly, is suitable for antibiosis and preservation of picked passion fruits, can effectively inhibit growth and reproduction of microorganisms of the picked passion fruits and prolong the preservation period of the passion fruits, and has the characteristics of being safe and environment-friendly at the same time.

The invention discloses an application of an RcARF8 gene in enhancing the resistance of Chinese rose to gray mold. The nucleotide sequence of the RcARF8 gene is shown as SEQ ID NO.1. The invention also discloses an application of the RcARF8 gene in enhancing the gray mold resistance of Chinese rose. The RcARF8 gene is silenced and overexpressed in petals which are good in growth state and free of botrytis cinerea infection of Chinese roses, and research results show that the petals treated by the silent RcARF8 gene are larger in scab area and lower in resistance to botrytis cinerea; the resistance of petals treated by overexpression of the RcARF8 gene to gray mold is enhanced.

The invention relates to a novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound, a preparation method thereof and application of the novel acridinyl-containing tetrahydronaphthalene spiro isoxazoline compound as an antifungal drug. The novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound has an inhibition effect on parasite sphaerospora, botrytis cinerea, rhizoctonia solani, fusarium graminearum and candida albicans in the antifungal activity of the novel acridinyl-containing tetrahydronaphthalene spiroisoxazoline compound. An antifungal biological activity result shows that the compound shows remarkable antifungal activity, has comprehensive antifungal characteristics and is determined as a broad-spectrum antifungal candidate, the EC50 value of the compound to parasitic powdery spores is superior to that of a positive control drug chlorothalonil, and the compound shows about 12 times and 25 times of titer advantages compared with famoxadone and carbendazim respectively. The spiro structure and multiple active groups of the compound can significantly improve the antifungal activity of the compound, and in addition, the compound has protection and treatment effects on powdery parasitic spore infection in corn, which shows that the compound has agricultural application potential.

The present invention relates to the use of a biological antimicrobial agent in antagonizing plant pathogenic fungi, preventing and controlling plant diseases, and preparing a plant disease control agent, and to the plant disease control agent, belonging to the technical field of microorganisms. The volatile metabolites of the fungus Rhacocystis albicans have a significant inhibitory effect on the growth of tomato gray mold, pepper alternating spot pathogen, and cucumber wilt pathogen, and can be used to antagonize plant pathogenic fungi (tomato gray mold, pepper alternating spot pathogen, and cucumber wilt pathogen), thereby avoiding the serious harm to human and animal health caused by pesticide residues when used.