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Efficient peanut rhizobiumleguminosarum strain and application thereof

A technology of rhizobacteria and nitrogen-fixing bacteria, applied in the field of microorganisms

Active Publication Date: 2014-02-12
河北浩海嘉农种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]In addition, the reasonable compounding of nitrogen-fixing bacterial strains and other beneficial strains can further enhance the nitrogen-fixing ability of plants, promote nutrient absorption and improve disease resistance and stress resistance, such as phosphorus dissolution Potassium microorganism Bacillus megaterium (ACCC10008 Bacillus megaterium) can secrete some growth-regulating substances to promote the absorption of nutrients and root growth in peanuts, but research in this area is still in the exploratory stage

Method used

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  • Efficient peanut rhizobiumleguminosarum strain and application thereof
  • Efficient peanut rhizobiumleguminosarum strain and application thereof
  • Efficient peanut rhizobiumleguminosarum strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, the separation and acquisition of nitrogen-fixing bacteria MSB1103 of the present invention

[0037] In July 2012, a peanut plant with nodules and abundant growth was collected at the peanut planting base in Weifang, Shandong, and the root nodules were isolated and purified: take fresh nodules, rinse them with water, and dry the surface water with filter paper; put the nodules in into 95% alcohol for 3 min, and then into 0.1% HgCl 2 medium, sterilize for 3-5 min, take it out and rinse it with sterile water for 5-6 times; place the sterilized root nodule on a sterilized glass slide and cut it in half, and clamp half of the nodule with sterile tweezers , the incision faces the surface of the YMA medium, and it is cultured in an inverted 28°C incubator. The composition of the YMA medium is: mannitol 10 g, K 2 HPO 4 0.25 g, MgSO 4 0.2 g, KH 2 PO 4 0.25 g, NaCl 0.1 g, CaCO 3 3 g, 3 g yeast powder, 15 g agar, 1000 mL distilled water.

Embodiment 2

[0038] Embodiment 2, inoculation identification

[0039] According to the description in Example 1, 11 strains of nitrogen-fixing bacteria were obtained through screening in the present invention, and the 11 strains of nitrogen-fixing bacteria were inoculated and identified. The test used a sterilized mixture of vermiculite and nutrient soil with a mass ratio of 1:1 as the culture substrate. Select plump peanut seeds, treat them in 75% alcohol for 1 min, wash them with water 5-6 times, then sterilize them with 0.1% mercuric chloride for 6 min, wash them with water 6-7 times, soak them in sterile water overnight until they imbibition. The imbibed seeds were germinated in sterilized Petri dishes at 25°C. When the radicle grows to 3-4 cm, transfer it to the sterilized culture medium. When the flower grows 4 cotyledons, add 2 mL of bacterial solution to each pot and place it in a light incubator for cultivation. Blank control. Three replicates were set up for each control and i...

Embodiment 3

[0042] Example 3, the colony morphology and physiological and biochemical identification of nitrogen-fixing bacteria MSB1103 of the present invention

[0043] Streak the nitrogen-fixing bacteria MSB1103 of the present invention on the YMA medium, and the resulting single colony form is as follows: figure 2 Shown: the colonies in the early stage of growth are translucent dots, and the colonies in the later stage are round, milky white, translucent, with neat edges. Gram tests such as image 3 As shown, the bacteria are Gram-negative bacteria without spores. Microscopic observation showed that the bacteria were rod-shaped, motile, and had flagella.

[0044] Physiological and biochemical identification of the obtained strains was carried out, including gelatin hydrolysis, contact enzyme test, nitrogen fixation ability and 5% NaCl, starch hydrolysis, indole production, citrate utilization, V-P determination, lecithin degradation and nitrate reduction experiments.

[0045] ...

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Abstract

The invention relates to a novel rhizobiumleguminosarum strain, wherein the strain has high biological nitrogen fixation activity, has certain preventive and therapeutic effects on fungal diseases of plants and has good saline-alkaline resistance and high temperature resistance, so that the strain has functions of nitrogen fixation, disease prevention, stress resistance and yield increase. The rhizobiumleguminosarum is preserved in the China General Microbiological Culture Collection Center Committee on July 15, 2013 with a preservation number of CGMCCNo.7924. As shown in researches, the strain can nodulate on peanut roots and the strain has a good inhibitory effect on botrytis cinerea and pythium aphanidermatum and has saline-alkaline resistance and high temperature resistance.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a nodule nitrogen-fixing bacterial strain with good nitrogen-fixing and antibacterial effects on peanuts and its application in preparing biological nitrogen-fixing bacterial fertilizers. Background technique [0002] Nitrogen is one of the most important nutrient elements for plant growth, and a large amount of nitrogen fertilizer is often required to increase crop yield. The growth of plants in the soil mainly relies on chemical fertilizers and biological nitrogen fixation to provide nitrogen sources. Due to the high production cost of chemical fertilizers, low utilization rate and huge impact on soil and environment, biological nitrogen fixation has become the focus of researchers. Biological nitrogen fixation is nitrogen-fixing microorganisms that convert nitrogen from the air to 2 Convert to NH 3 process that is efficient, environmentally friendly and cost-ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C05G3/00C12R1/41
Inventor 赵巍赵斌董金皋
Owner 河北浩海嘉农种业有限公司
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