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Penicillium brefeldianum strain and application thereof

A technology of penicillium and bacterial strains, applied in the direction of fungi, fungicides, chemicals for biological control, etc., can solve the problems of unsatisfactory control effect of chemical pesticides, environmental pollution, etc., achieve good control effect and reduce the cost of use Effect

Active Publication Date: 2020-01-10
云南省农业科学院生物技术与种质资源研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the current serious occurrence of Panax notoginseng root rot and heavy fungus diseases, and the unsatisfactory control effect of chemical pesticides, and the problems of environmental pollution, we provide a plant that can effectively prevent and control Panax notoginseng root rot, Botrytis cinerea and leaf mold. Penicillium brefeldianum (Penicillium brefeldianum) bacterial strain of leaf spot and application thereof

Method used

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  • Penicillium brefeldianum strain and application thereof
  • Penicillium brefeldianum strain and application thereof
  • Penicillium brefeldianum strain and application thereof

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Embodiment 1

[0029] The Penicillium brefeldianum strain of the present invention, the Penicillium brefeldianum strain is PFLJ04, and the preservation name is Penicillium brefeldianum (Penicillium brefeldianum), which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the depository unit The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 15, 2019; Collection number: CGMCC No.18119.

[0030] The ITS gene sequence of the said Penicillium brazenii strain is the nucleotide sequence shown in SEQ ID No.1.

[0031] The Penicillium brescenium inoculum prepared by the Penicillium brescens strain of the present invention. Its active ingredient is at least one of the following (a) (b) (c):

[0032] (a) the fermented culture of described Penicillium breezii bacterial strain;

[0033] (b) the ultrasonic lysis supernatant of the obtained Penicillium brezhengii bacterial stra...

Embodiment 2

[0042] The difference between embodiment 2 and embodiment 1 is: the preparation method of the fermentation liquid of Penicillium brezhenii according to the present invention comprises the following steps: inoculating the described bacterial strain in the PDA culture medium dish, 28 ℃ upside-down culture for 7 days , activate the bacterial strain, use distilled water to prepare the activated bacterial strain into a spore suspension, count the spore concentration under a microscope, and the concentration of the spore suspension is: 1.0×10 6 cells / mL; inoculate 5% spore suspension in liquid medium PDB, culture at 28°C, 200r / min, for 7 days.

[0043] The PDA medium is composed of the following components: 200g of potatoes, 5g of glucose, 10g of agar, distilled to 1L of distilled water, natural pH, sterilized at 121°C for 30min; the PDB medium is composed of the following components: potatoes 200g, glucose 5g, dilute to 1L distilled water, pH 8, sterilize at 121°C for 30min.

[00...

Embodiment 3

[0046] The difference between embodiment 3 and embodiment 1 is: the preparation method of the fermentation liquid of Penicillium brezhenii according to the present invention comprises the following steps: inoculating the described bacterial strain in the PDA culture medium dish, 28 ℃ upside-down culture 7 days , activate the bacterial strain, use distilled water to prepare the activated bacterial strain into a spore suspension, count the spore concentration under a microscope, and the concentration of the spore suspension is: 8.0×10 4 Individuals / mL; 5% spore suspension was inoculated in liquid medium PDB, cultured at 28° C., 200 r / min, for 9 days.

[0047] The PDA medium is composed of the following components: 180g of potatoes, 15g of glucose, 20g of agar, distilled to 1L of distilled water, natural pH, sterilized at 121°C for 30min; the PDB medium is composed of the following components: potatoes 100g, glucose 20g, dilute to 1L distilled water, pH 7, sterilize at 121°C for ...

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Abstract

The invention discloses a penicillium brefeldianum strain and application thereof. The penicillium brefeldianum strain is PFLJ04, the preservation name of the strain is penicillium brefeldianum, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation date is August 15, 2019; and the preservation number is CGMCC No. 18119. Penicillium is separated from paris polyphylla var. yunnanensis endophyte, and has a good inhibiting effect on panax notoginseng root rot fungi (fusarium oxysporum, fusarium solani and phytophthoracactorum), paris polyphylla leaf spot fungi and paris polyphylla botrytis cinerea (botrytis cinerea, phoma sp. and pestalotiopsis sp.) simultaneously.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a strain of Penicillium brezenii and its application. Background technique [0002] In recent years, with the sharp increase in the demand for traditional Chinese medicine resources, the wild resources of traditional Chinese medicine have been severely damaged, and the transformation of wild resources into cultivation has become the only way to meet market demand. Diseases rarely occur in wild Chinese medicinal materials, and even if they do, they will not cause disasters. After changing from wild to artificial cultivation, their growth environment changes. Management measures such as seed transfer have created the conditions for the occurrence of diseases, and diseases are easy to spread within the population, between fields, and between regions, resulting in aggravated diseases of cultivated medicinal materials. [0003] Sanqi (Panax notoginseng (Burk.) F.H.Chen)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/06A01P3/00C12R1/80
CPCC12N1/06C12N1/14C12N1/145C12R2001/80
Inventor 张仲凯郑宽瑜陈永对张洁郑雪赵立华吴阔苏晓霞
Owner 云南省农业科学院生物技术与种质资源研究所
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