Hanseniaspora vineae capable of effectively preventing and controlling postharvest diseases of fruits and vegetables and preparation and use method thereof

A technology for postharvest diseases and wine, applied in the field of biological control of postharvest diseases of fruits and vegetables, can solve the problems that the biocontrol effect is only verified on a small number of fruits, lack of strains with antibacterial spectrum, etc., and achieve significant social and ecological benefits and stable traits , the effect of reducing the incidence

Active Publication Date: 2018-04-03
山东凯普菲特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Hanseniaspora vineae capable of effectively preventing and controlling postharvest diseases of fruits and vegetables and preparation and use method thereof
  • Hanseniaspora vineae capable of effectively preventing and controlling postharvest diseases of fruits and vegetables and preparation and use method thereof
  • Hanseniaspora vineae capable of effectively preventing and controlling postharvest diseases of fruits and vegetables and preparation and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Biological characteristics of wine-sporulated yeast Hanseniaspora vineae BY19

[0020] 1. Morphological features

[0021] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0022] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0023] 2. Molecular biological identification

[0024] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to perform PCR amplification of yeast 26S rDNA D1 / D2 region nucleic acid sequence, and the PCR product The sequencing results were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downl...

Embodiment 2

[0026] Implementation example 2 Inhibitory effect of Hansula spp. BY19 on apple penicillium and botrytis cinerea

[0027] 1. Experimental protocol

[0028] Take Han. spp. BY19 out of the refrigerator at -80°C, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), Pick a single colony into the YPD liquid medium, culture it at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, and count on a hemocytometer to prepare Prepared to a concentration of 1 x 10 8 Cells / mL of Hansula sporogenes BY19 suspension in wine.

[0029]Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinere...

Embodiment 3

[0036] Implementation example 3 Inhibitory effect of Hansula sporogenes BY19 on pear fruit blue mold and gray mold

[0037] 1. Experimental protocol

[0038] Take Han. spp. BY19 out of the refrigerator at -80°C, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), Pick a single colony into the YPD liquid medium, culture it at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, and count on a hemocytometer to prepare Prepared to a concentration of 1 x 10 8 Cells / mL of Hansula sporogenes BY19 suspension in wine.

[0039] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cin...

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PUM

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Abstract

The invention discloses a hanseniaspora vineae BY19 for preventing and controlling postharvest diseases of fruits and vegetables and with wide antibacterial spectrum and stable effect and a use methodthereof. The collection number of the strain in China General Microbiological Culture Collection Center is CGMCC No. 14903. The use method of the hanseniaspora vineae comprises the following steps of: activating the strain, carrying out fermentation culture by using YPD, centrifuging, and preparing thalli into 1*108 cell/mL of bacterial suspension by using sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citruses or cherry tomatoes and the like into the bacterial suspension, soaking for 30 seconds, then taking out, and carrying out air drying; puttinginto a fresh-keeping box, and storing at normal temperature. The hanseniaspora vineae strain disclosed by the invention has the beneficial effects that penicilliosis and botrytis cinerea of the apples, the penicilliosis and the botrytis cinerea of the pears, botrytis, aspergillosis, black spot, fusaridiosis and anthracnose of the grapes, the botrytis of the strawberries and the botrytis and the aspergillosis of the cherry tomatoes can be controlled simultaneously, so that the loss caused by the postharvest diseases is reduced and the application prospect is good.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits and vegetables, in particular to a strain of Hanseniaspora vineae used for biological control of post-harvest diseases of fruits and vegetables. The main postharvest diseases of the female fruit have significant control effects. Background technique [0002] The quality deterioration of fresh fruit and vegetable products is affected by many factors, but the rot and deterioration caused by diseases, especially fungal diseases, is the most serious factor in the postharvest loss of fruits. Although postharvest diseases of fruits and vegetables can be controlled through many methods such as agricultural control, physical control, chemical control and biological control, the main measure to control postharvest diseases of fruits and vegetables is chemical control (Eckert & Ogawa, 1985, 1988). However, the long-term use of chemical pesticides not only leads to the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155C12N1/16C12N1/145C12R2001/645
Inventor 王友升姚婷陈滢范雅为黄津津王颖
Owner 山东凯普菲特生物科技有限公司
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