Hanseniaspora vineae capable of effectively preventing and controlling postharvest diseases of fruits and vegetables and preparation and use method thereof
A technology for postharvest diseases and wine, applied in the field of biological control of postharvest diseases of fruits and vegetables, can solve the problems that the biocontrol effect is only verified on a small number of fruits, lack of strains with antibacterial spectrum, etc., and achieve significant social and ecological benefits and stable traits , the effect of reducing the incidence
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Example 1: Biological characteristics of wine-sporulated yeast Hanseniaspora vineae BY19
[0020] 1. Morphological features
[0021] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.
[0022] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.
[0023] 2. Molecular biological identification
[0024] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to perform PCR amplification of yeast 26S rDNA D1 / D2 region nucleic acid sequence, and the PCR product The sequencing results were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downl...
Embodiment 2
[0026] Implementation example 2 Inhibitory effect of Hansula spp. BY19 on apple penicillium and botrytis cinerea
[0027] 1. Experimental protocol
[0028] Take Han. spp. BY19 out of the refrigerator at -80°C, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), Pick a single colony into the YPD liquid medium, culture it at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, and count on a hemocytometer to prepare Prepared to a concentration of 1 x 10 8 Cells / mL of Hansula sporogenes BY19 suspension in wine.
[0029]Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinere...
Embodiment 3
[0036] Implementation example 3 Inhibitory effect of Hansula sporogenes BY19 on pear fruit blue mold and gray mold
[0037] 1. Experimental protocol
[0038] Take Han. spp. BY19 out of the refrigerator at -80°C, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), Pick a single colony into the YPD liquid medium, culture it at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, and count on a hemocytometer to prepare Prepared to a concentration of 1 x 10 8 Cells / mL of Hansula sporogenes BY19 suspension in wine.
[0039] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cin...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com